questionnaire and sampling are scientifically designed, middle and senior management staff of the enterprises are required to fill in the questionnaire, as to ensure the feedback questionnaire of high-quality, which shows that the key issues to Guangzhou enterprise’s innovation and venturing includes: talents, capital, technology, market, policy support and intellectual property protection. The main bottlenecks and its causes of each key issue are revealed in this article, countermeasures and suggestions are specifically proposed as well.