| 不同激素配比对铁皮石斛组织培养的影响研究 |
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| 引用本文： | 陈兆贵,谭俊.不同激素配比对铁皮石斛组织培养的影响研究[J].惠州学院学报,2006,26(3):11-14. |
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| 作者姓名： | 陈兆贵 谭俊 |
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| 作者单位： | 惠州学院,生命科学系,广东,惠州,516007 |
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| 基金项目： | 惠州学院校科研和教改项目;惠州学院校科研和教改项目 |
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| 摘 要： | 在铁皮石斛组织培养过程中,通过筛选适宜铁皮石斛原球茎增殖、分化、壮苗和生根的培养基,以提高增殖系数和试管苗的质量。结果表明:原球茎增殖以MS NAA1．0 mg／L 6-BA1．0 mg／L KT1．0 mg／L为最佳;原球茎分化以MS NAA1．0 mg／L 6-BA3．0mg／L KT1．0 mg／L为最佳;壮苗生根以1／2MS IBA2．0 mg／L 水解酪蛋白500mg／L 10％香蕉提取液为最佳,45天生根率达92％。
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| 关 键 词： | 铁皮石斛 组织培养 激素 原球茎 |
| 文章编号： | 1671-5934（2006）03-0011-04 |
| 收稿时间： | 2006-02-12 |
| 修稿时间： | 2006-02-12 |
Effects of Different Hormone Proportions on Tissue Culture of Dendrobium Officinale |
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| CHEN Zhao-gui,TAN Jun.Effects of Different Hormone Proportions on Tissue Culture of Dendrobium Officinale[J].Journal of Huizhou University,2006,26(3):11-14. |
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| Authors: | CHEN Zhao-gui TAN Jun |
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| Affiliation: | Department of Life Science, Huizhou University, Huizhou 516007, Guangdong China |
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| Abstract: | The study was aimed at selecting the optimum culture media for protocorm multiplication,differentiation, rooting and seedling strengthening of dendrobium officinale to raise the coefficient of succesivertansfer multiplication and the quality of plant. The result showed that MS plus (NAA 1.0 mg/L 6- BA 3.0 mg/ KT 1.0 mg/L)is the best media for protocorm multiplication. MS adding (NAA1.0 mg/L 6 - BA3.0 mg/1 KT1 .0 mg/L) is the best media for protocorm differentiation. 1/2 MS plus IBA2.0 mg/1 10% banana juice casein hydroly - sate is the best media for rooting and seedling strengthening, and the growth rate reached 92% after 45 days. |
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| Keywords: | dendrobium qfficinale tissue culture hormone protocorm |
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