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1.
Hemolysis of erythrocytes from human and porcine blood was studied at different calcium chloride (CaCl2) concentrations (0.04–1.83 mM) and at a constant bilirubin concentration (72 mM). Although, human erythrocytes showed significant
hemolysis (64%) at the highest CaCl2 concentration (1.83 mM) used in this study, remarkable resistance to this phenomenon was observed with porcine erythrocytes
as only 11% hemolysis was observed. A similar pattern in the behavior of both human and porcine erythrocytes was observed
when parameter such as bilirubin concentration or time of incubation with bilirubin was varied. Other divalent cations such
as Mn2+, Ba2+ and Mg2+ were either least effective or ineffective in inducing hemolysis in presence of bilirubin. Serum albumin played a protective
role in this phenomenon in a concentration dependent manner, as no hemolysis was observed at a bilirubin/albumin molar ratio
of 1:1 or less. Differences in the structural make-up of proteins and lipids in the erythrocyte membranes of the two species
may account for their different behavior. 相似文献
2.
为掌握百色市山羊布鲁氏菌病及衣原体病感染状况。在2013年对百色市辖12个县区的养羊场进行调查并采集血清进行布病及衣原体检测。调查发现,1732个羊场中有159个发生流产、死胎的现象。431个存栏100只以上的羊场中,有87个发生过流产、死胎现象。对76个羊场采集924份血清样品使用虎红平板凝集试验及试管凝集试验进行检测,未检出布鲁氏菌阳性血清。送检520份血清,检出衣原体阳性血清52份,阳性率10%;其中检测种母羊血清139份,检出衣原体阳性血清14份,阳性率10.07%;检测种公羊血清72份,检出衣原体阳性血清9份,阳性率12.50%。经分析,百色市山羊的布病防控达到广西稳定控制标准要求;羊衣原体病在本场乃至整个百色地区有蔓延流行的风险。 相似文献
3.
Gabriel Lima-Oliveira Gian Luca Salvagno Elisa Danese Giorgio Brocco Gian Cesare Guidi Giuseppe Lippi 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2014,24(3):359-367
Introduction:
The contamination of serum or lithium heparin blood with ethylenediaminetetraacetic acid (EDTA) salts may affect accuracy of some critical analytes and jeopardize patient safety. The aim of this study was to evaluate the effect of lithium heparin sample contamination with different amounts of K2EDTA.Materials and methods:
Fifteen volunteers were enrolled among the laboratory staff. Two lithium heparin tubes and one K2EDTA tube were collected from each subject. The lithium-heparin tubes of each subject were pooled and divided in 5 aliquots. The whole blood of K2EDTA tube was then added in scalar amount to autologous heparinised aliquots, to obtained different degrees of K2EDTA blood volume contamination (0%; 5%; 13%; 29%; 43%). The following clinical chemistry parameters were then measured in centrifuged aliquots: alanine aminotranspherase (ALT), bilirubin (total), calcium, chloride, creatinine, iron, lactate dehydrogenase (LD), lipase, magnesium, phosphate, potassium, sodium.Results:
A significant variation starting from 5% K2EDTA contamination was observed for calcium, chloride, iron, LD, magnesium (all decreased) and potassium (increased). The variation of phosphate and sodium (both increased) was significant after 13% and 29% K2EDTA contamination, respectively. The values of ALT, bilirubin, creatinine and lipase remained unchanged up to 43% K2EDTA contamination. When variations were compared with desirable quality specifications, the bias was significant for calcium, chloride, LD, magnesium and potassium (from 5% K2EDTA contamination), sodium, phosphate and iron (from 29% K2EDTA contamination).Conclusions:
The concentration of calcium, magnesium, potassium, chloride and LD appears to be dramatically biased by even modest K2EDTA contamination (i.e., 5%). The values of iron, phosphate, and sodium are still reliable up to 29% K2EDTA contamination, whereas ALT, bilirubin, creatinine and lipase appear overall less vulnerable towards K2EDTA contamination. 相似文献4.
Ca++ was not found to be essential for the activation of the latent elastase in normal lenses. However, the activities increased 70–100% in human, bovine, sheep and goat lenses at a concentration of 5.0×10?3M of CaCl2. On the other hand, in cataractous human lenses, Ca++ inhibited the process of activation. The activation process however, seems to be a divalent metal ion dependent process since ethylene diamine tetra acetate (2.0×10?3M) blocked the activation of the elastase-like enzyme in both normal and cataractous lenses. p-Hydroxy mercuribenzoate (10?4M) and benzamidine (1.65×10?2M) also blocked the activation process, indicating that a trypsin-like enzyme requiring sulfhydryl groups, is involved in the activation of latent elastase. 相似文献
5.
Hori Ghaneialvar Leila Soltani Hamid Reza Rahmani Abbas Sahebghadam Lotfi Masoud Soleimani 《Indian journal of clinical biochemistry : IJCB》2018,33(1):46-52
Mesenchymal stem cells are multipotent cells capable of replicating as undifferentiated cells, and have the potential of differentiating into mesenchymal tissue lineages such as osteocytes, adipocytes and chondrocytes. Such lineages can then be used in cell therapy. The aim of present study was to characterize bone marrow derived mesenchymal stem cells in four different species, including: sheep, goat, human and mouse. Human bone-marrow mesenchymal stem cells were purchased, those of sheep and goat were isolated from fetal bone marrow, and those of mouse were collected by washing bone cavity of femur and tibia with DMEM/F12. Using flow-cytometry, they were characterized by CD surface antigens. Furthermore, cells of third passage were examined for their osteogenic and adipogenic differentiation potential by oil red and alizarin red staining respectively. According to the results, CD markers studied in the four groups of mesenchymal stem cells showed a different expression. Goat and sheep expressed CD44 and CD166, and weakly expressed CD34, CD45, CD105 and CD90. Similarly, human and mouse mesenchymal cells expressed CD44, CD166, CD105 and CD90 whereas the expression of CD34 and CD45 was negative. In conclusion, although all mesenchymal stem cells display plastic adherence and tri-lineage differentiation, not all express the same panel of surface antigens described for human mesenchymal stem cells. Additional panel of CD markers are necessary to characterize regenerative potential and possible application of these stem cells in regenerative medicine and implantology. 相似文献
6.
Prasheeda Chandran Pradeep Garg Chandra S. Pundir 《Indian journal of clinical biochemistry : IJCB》2005,20(2):81-85
Total cholesterol, total bilirubin, calcium, oxalate, inorganic phosphate, magnesium, iron, copper, sodium and potassium were
analyzed quantitatively in gallstones, bile of gall bladder and sera of 200 patients of cholelithiasis (52 cholesterol, 76
mixed and 72 pigment stone patients) and their contents were correlated between calculi and bile and sera and bile in these
three type of stone patients. A significant positive correlation was observed between total cholesterol, total bilirubin of
calculi and bile, copper of bile and sera of cholesterol stone patients, copper of calculi and bile, total bilirubin, oxalate,
magnesium, potassium of sera and bile of pigment stone patients and oxalate and iron of stone and bile, total bilirubin, oxalate,
sodium of sera and bile of mixed stone patients. A significant negative correlation was found between magnesium of serum and
bile of cholesterol stone patients, oxalate of calculi and bile of pigment stone patients and magnesium of serum and bile
of mixed stone patients. 相似文献
7.
P. Chandran N. K. Kuchhal P. Garg C. S. Pundir 《Indian journal of clinical biochemistry : IJCB》2007,22(2):145-150
Chemical composition of gall stones is essential for aetiopathogensis of gallstone disease. We have reported quantitative
chemical analysis of total cholesterol bilirubin, calcium, iron and inorganic phosphate in 120 gallstones from haryana. To
extend this chemical analysis of gall stones by studying more cases and by analyzing more chemical constituents. A quantitative
chemical analysis of total cholesterol, total bilirubin, fatty acids, triglycerides, phospholipids, bile acids, soluble proteins,
sodium potassium, magnesium, copper, oxalate and chlorides of biliary calculi (52 cholesterol, 76 mixed and 72 pigment) retrieved
from surgical operation of 200 patients from Haryana state was carried out. Total cholesterol as the major component and total
bilirubin, phospholipids, triglycerides, bile acids, fatty acids (esterified), soluble protein, calcium, magnesium, iron,
copper, sodium, potassium, inorganic phosphate, oxalate and chloride as minor components were found in all types of calculi.
The cholesterol stones had higher content of total cholesterol, phospholipids, fatty acids (esterified), inorganic phosphate
and copper compared to mixed and pigment stones. The mixed stones had higher content of iron and triglycerides than to cholesterol
and pigment stones. The pigment stones were richer in total bilirubin, bile acids, calcium, oxalate, magnesium, sodium, potassium,
chloride and soluble protein compared to cholesterol and mixed stones. Although total cholesterol was a major component of
cholesterol, mixed and pigment gall stone in Haryana, the content of most of the other lipids, cations and anions was different
in different gall stones indicating their different mechanism of formation. 相似文献
8.
Pushpa Durgawale P. S. Shukla Sonali Mishra 《Indian journal of clinical biochemistry : IJCB》1999,14(2):241-244
An unexpected increase in erythrocyte osmotic resistance during viral hepatitis in two patients prompted study of effects
of hepatitis (in Karad) on osmotic resistance. The test was performed by placing erythrocytes in saline solutions of decreasing
osmolarity and osmotic fragility defined in terms of the saline concentration at which hemolysis begins. Study included 44
infected subjects (acute viral hepatitis non-A non-B). All forty four infected subjects showed abnormal results when compared
to normal subjects. increased erythrocyte osmotic resistance i.e. hemolysis begins between 0.45% to 0.40% of NaCl and is completed
between 0.25% to 0.20% NaCl, whereas in normal subjects. hemolysis begins between 0.50% to 0.45% NaCl and is completed between
0.35% to 0.30% NaCl. Osmotic resistance was observed with increase in total bilirubin (mean±SD) (4.6 ±3.6), direct bilirubin
(3.0±2.5), SGOT (58.8±55.5) SGPT (114.2±150.3) activity. Physiologic shifts in erythrocyte osmotic resistance may be due to
changes in membrane lipid ratio. 相似文献
9.
Chuanyin Li Wenli Lu Liguang Yang Zhengwei Li Xiaoyi Zhou Rong Guo Junqi Wang Zhebao Wu Zhiya Dong Guang Ning Yujiang Shi Yinmin Gu Peng Chen Zijian Hao Tianting Han Meiqiang Yang Wei Wang Xuehui Huang Yixue Li Shan Gao Ronggui Hu 《国家科学评论(英文版)》2020,7(3):671
Central precocious puberty (CPP) refers to a human syndrome of early puberty initiation with characteristic increase in hypothalamic production and release of gonadotropin-releasing hormone (GnRH). Previously, loss-of-function mutations in human MKRN3, encoding a putative E3 ubiquitin ligase, were found to contribute to about 30% of cases of familial CPP. MKRN3 was thereby suggested to serve as a ‘brake’ of mammalian puberty onset, but the underlying mechanisms remain as yet unknown. Here, we report that genetic ablation of Mkrn3 did accelerate mouse puberty onset with increased production of hypothalamic GnRH1. MKRN3 interacts with and ubiquitinates MBD3, which epigenetically silences GNRH1 through disrupting the MBD3 binding to the GNRH1 promoter and recruitment of DNA demethylase TET2. Our findings have thus delineated a molecular mechanism through which the MKRN3–MBD3 axis controls the epigenetic switch in the onset of mammalian puberty. 相似文献
10.
《Electronic Journal of Biotechnology》2014,17(4):168-173
BackgroundGenetic diversity of sheep in Jordan was investigated using microsatellite markers (MS). Six ovine and bovine MS located on chromosomes 2 and 6 of sheep genome were genotyped on 294 individual from ten geographical regions.ResultsThe number of alleles per locus (A), the expected heterozygosity (He) and observed heterozygosity (Ho) were measured. Overall A, He and Ho were 12.67, 0.820 and 0.684, respectively. On the other hand, genetic distances undoubtedly revealed the expected degree of differentiation among the studied populations. The finding showed closeness of three populations from south (Maan, Showbak and Tafeilah) to each other. Populations from the middle regions of Jordan (Karak, Madaba, Amman, AzZarqa and Mafraq) were found to be in one cluster. Only two populations of the middle region were an exception: AlSalt and Dead Sea. Finally, sheep populations from Irbid were located in separated cluster. It was clear that the studied predefined populations were subdivided from four populations and would be most probably accounted as ancestral populations. These results indicate that number of population is less than the predefined population as ten based on geographical sampling areas.ConclusionsThe possible inference might be that geographical location, genetic migration, similar selection forces, and common ancestor account for population admixture and subdivision of Awassi sheep breed in Jordan. Finally, the present study sheds new light on the molecular and population genetics of Awassi sheep from different regions of Jordan and to utilize the possible findings for future management of genetic conservation under conditions of climate changes and crossbreeding policy. 相似文献
11.
CH. V. Ramana Devi M. P. J. S. Anandaraj 《Indian journal of clinical biochemistry : IJCB》1994,9(1):21-23
The enhanced presence of calcium in the erythrocytes of thalassemia homozygotes along with an increase in cytosolic and membrane
bound CANP, and a decrease in membrane glycoprotein and total membrane sulfhydryl groups are statistically significant changes.In vitro loading of erythrocytes with calcium in the presence of lonophore A23187 caused similar changes pointing to the significance of calcium mediated proteolysis, especially by CANP in preparing erythrocytes
for the phagocytic event. 相似文献
12.
Tingting Wang Dong Wei Xien Chang Zhiyong Yu Xinyu Zhang Changsui Wang Yaowu Hu Benjamin T Fuller 《国家科学评论(英文版)》2019,6(5):1024
The westward expansion of human millet consumption from north China has important implications for understanding early interactions between the East and West. However, few studies have focused on the Xinjiang Uyghur Autonomous Region, the vast geographical area directly linking the ancient cultures of the Eurasian Steppe and the Gansu Corridor of China. In this study, we present the largest isotopic investigation of Bronze Age China (n = 110) on material from the key site of Tianshanbeilu, in eastern Xinjiang. The large range of δ13C values (–17.6‰ to –7.2‰; –15.5 ± 1.2‰) provides direct evidence of unique dietary diversity and consumption of significant C4 resources (millets). The high δ15N results (10.3‰ to 16.7‰; 14.7 ± 0.8‰) likely reflect sheep/goat and wild game consumption and the arid climate of the Taklamakan Desert. Radiocarbon dates from four individuals indicate Tianshanbeilu was in use between 1940 and 1215 cal bc. The Tianshanbeilu results are then analysed with respect to 52 Bronze Age sites from across Eurasia, to investigate the spread and chronology of significant human millet consumption and human migration. This isotopic survey finds novel evidence that the second millennium bc was a dynamic period, with significant dietary interconnectivity occurring between north China, Central Asia and Siberia. Further, we argue that this ‘Isotopic Millet Road’ extended all the way to the Mediterranean and Central Europe, and conclude that these C4 dietary signatures of millet consumption reflect early links (migration and/or resource transfer) between the Bronze Age inhabitants of modern-day China and Europe. 相似文献
13.
Palani Damotharan Anguchamy Veeruraj Muthuvel Arumugam Thangavel Balasubramanian 《Indian journal of clinical biochemistry : IJCB》2016,31(1):57-67
This study is designed to isolate and purify a novel anti-clotting protein component from the venom of Enhydrina schistosa, and explore its biochemical and biological activities. The active protein was purified from the venom of E. schistosa by ion-exchange chromatography using DEAE-cellulose. The venom protein was tested by various parameters such as, proteolytic, haemolytic, phospholipase and anti-coagulant activities. 80 % purity was obtained in the final stage of purification and the purity level of venom was revealed as a single protein band of about 44 kDa in SDS-polyacrylamide electrophoresis under reducing conditions. The results showed that the Potent hemolytic activity was observed against cow, goat, chicken and human (A, B and O positive) erythrocytes. Furthermore, the clotting assays showed that the venom of E. schistosa significantly prolonged in activated partial thromboplastin time, thrombin time, and prothrombin time. Venomous enzymes which hydrolyzed casein and gelatin substrate were found in this venom protein. Gelatinolytic activity was optimal at pH 5–9 and 1H NMR analysis of purified venom was the base line information for the structural determination. These results suggested that the E. schistosa venom holds good promise for the development of novel lead compounds for pharmacological applications in near future. 相似文献
14.
The structure and function of biological systems, for example, cells and proteins, depend strongly on their chemical environment. To investigate such dependence, we design a polydimethylsiloxane-based microfluidic device to encapsulate biological systems in picoliter-sized drops. The content of each individual drop is tuned in a defined manner. As a key feature of our method, the individual chemical composition is determined and related to the drop content. In our case, the drop content is imaged using microscopy methods, while the drops are immobilized to allow for long-time studies. As an application of our device, we study the influence of divalent ions on vimentin intermediate filament networks in a quantitative way by tuning the magnesium concentration from drop to drop. This way we are able to directly image the effect of magnesium on the fluorescently tagged protein in a few hundreds of drops. Our study shows that with increasing magnesium concentration in the drops, the compaction of the networks becomes more pronounced. The degree of compaction is characterized by different morphologies; freely fluctuating networks are observed at comparatively low magnesium concentrations of 5–10 mM, while with increasing magnesium concentration reaching 16 mM they develop into fully aggregated networks. Our approach demonstrates how a systematic study of interactions in biological systems can benefit from the exceptional controllability of microfluidic methods. 相似文献
15.
16.
Chethana Chetan Ashok Prabhu Vivian D'Souza Shrinivas B. Rao 《Indian journal of clinical biochemistry : IJCB》2000,15(1):17-21
Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against
the peroxidative action of H2O2 which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations
used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all
the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress
haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines
may have independent mechanism of action. 相似文献
17.
Kaushik Kar Anindya Dasgupta M. Vijaya Bhaskar K. Sudhakar 《Indian journal of clinical biochemistry : IJCB》2014,29(2):232-237
Decompensation followed by death is the most serious outcome in patients suffering from cirrhosis of the liver. Alteration of trace elements may play a vital role in the process of decompensation. To examine the change in status of trace elements during the decompensation process, we analysed the zinc, copper, iron, magnesium, bilirubin and albumin levels in the serum of compensated (n = 34) and decompensated (n = 31) liver cirrhosis patients and compared them with healthy control group (n = 36) by post hoc ANOVA. We observed significant alteration in the selected micronutrients in the diseased group relative to healthy controls (P < 0.05). Moreover, mean serum zinc and iron levels were significantly lower with a higher level of serum copper in decompensated cirrhosis group than in compensated group (P < 0.05). However, no significant decrease of serum magnesium was found between the two diseased groups. Our findings imply that the trace elements like zinc, copper and iron might exert important contributory roles in decompensation process in liver cirrhosis and hence, may be utilized as important biomarkers for these patients. Furthermore, we propose that replacements of those micronutrients at an early stage can delay or prevent the severe outcomes like hepatic encephalopathy, gastrointestinal bleeding, severe jaundice or ascites in these patients. 相似文献
18.
Prabhat Kumar Nigam 《Indian journal of clinical biochemistry : IJCB》2016,31(2):237-239
The negative interference by bilirubin in serum creatinine estimation by Jaffe’s kinetic method is well known. Several approaches have been suggested to overcome this interference. In this article three different creatinine kits (Jaffe’s kinetic method) have been tested for bilirubin interference and its rectification using two simple approaches. The performance of three kits (A, B and C) supplied by three different manufacturers was tested using IQC and EQAS sera and pooled serum with added bilirubin. To overcome the bilirubin interference two approaches viz. NaOH preincubation and TCA precipitation were used. Bilirubin did interfere in creatinine estimation after a certain level (2.3 mg/dl). However, both NaOH preincubation and TCA precipitation approach rectified this interference. The performance of kit A was better than kit B and C. All the three kits showed bilirubin interference upon increasing the bilirubin concentration but kit A performed better than kit B and C. However, NaOH incubation and TCA precipitation methods overcame this interference to a great extent. 相似文献
19.
Rufino Mondejar María Mayor Reyes Enrique Melguizo Madrid Consuelo Caavate Solano Santiago Prez Ramos 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2021,31(2)
IntroductionTotal bilirubin tests are highly demanded in clinical laboratories. Since icteric index (I-index) has zero cost, we aimed to evaluate its clinical utility and cost-effectiveness to determine if total bilirubin is necessary to be tested. We took into account if haemolysis could interfere to icteric index determination.Material and methodsRetrospectively we reviewed I-index results in two cohorts (43,372 and 8507 non-haemolysed and haemolysed samples, respectively). All determinations were done using Alinity c chemistry analysers (Abbott Diagnostics). Receiver operating characteristic (ROC) curve was used to determine the optimal index cut-off to discriminate between normal and abnormal bilirubin concentration (20.5 µmol/L).ResultsThe ROC curve analysis suggested 21.4 µmol/L as the optimal I-index cut-off but differences in sensitivity and specificity were detected between patient derivation. For rejecting purpose, 15.4 µmol/L and 17.1 µmol/L I-index thresholds were selected based on patient derivation (inpatients and emergency room; and primary care and outpatients, respectively) with 97% sensitivity and 0.25% false negative results. Sensitivity was much lower in haemolysed samples. We selected 34.2 µmol/L I-index as threshold to detect hyperbilirubinemia with 99.7% specificity and 0.26% false positive results, independent of haemolysis. With the icteric index cut-offs proposed, we would save 66% of total bilirubin requested and analyse total bilirubin in around 2% of samples without total bilirubin requested.ConclusionsThis study supports the use of I-index to avoid bilirubin determination and to identify patients with hyperbilirubinemia. This work considers that the economic and test savings could help to increase the efficiency in clinical laboratories. 相似文献
20.
M. H. Meshkibaf A. Ebrahimi R. Ghodsi A. Ahmadi 《Indian journal of clinical biochemistry : IJCB》2006,21(1):161-164
A number of newly developed antiepileptic drugs are currently in use, among them Lamotrigine (LTG) is more common. Despite
the extensive use of this drug, it has not been possible to predict the side effects especially the hepatotoxic reactions
after long-term treatment. The present study was designed to find out alterations in the activities of liver enzymes after
chronic exposure of rats to different dose of LTG. Adults male (Wistar) rats were treated orally with LTG [5 mg/kg body weight
or 25 mg/kg body wt.] for 60 days. After the experimental period, auto analyzer carried out liver function tests. The liver
histopathology was obtained after scarifying the rats. There was a significant increase in the level of ALP, AST, ALT and
bilirubin at therapeutic dose of LTG. The increase level of these enzymes and bilirubin at toxic dose were much higher and
significant. However, the total protein and albumin significantly decreased at toxic dose of LTG. Elevation of liver enzymes
and bilirubin after chronic exposure of rats to high dose of LTG reflects hepatocellular damage that may lead to hepatitis.
It is concluded that regular liver function and drug monitoring should follow the treatment with LTG. 相似文献