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1.
通过对里氏木霉ACCC30449分泌的纤维素酶酶解条件比较,初步发现该菌株产生的纤维素酶的最适作用pH值为5,利用菌株产纤维素酶分解处理不同比例搭配的玉米秸杆和稻草,结果表明玉米秸杆:稻草= 3:1的时候,纤维素的分解速率最高。  相似文献   

2.
同步固态发酵玉米秸秆生产单细胞蛋白的研究   总被引:2,自引:0,他引:2  
同步固态发酵中,采用单因素及正交试验对发酵条件和工艺参数进行了研究,研究表明:在纤维素酶曲用量511U/g(FPA),加水比1:2~1:3的情况下,接种产朊假丝酵母10%,30C发酵48h粗蛋白含量由原料秸秆的0.86%提高到7.40%。  相似文献   

3.
利用选择性培养基从土壤中分离具有产壳聚糖酶能力的菌株,获得两株产壳聚糖酶菌株,对其中的一株非芽孢杆菌Yg菌株进行产酶试验并对其酶活性以及酶解最适条件进行测定.结果显示:Yg菌株所产壳聚糖酶具有较强的降解壳聚糖的能力,不同产酶时间所得酶液中,产酶36h所得酶液的酶活力最强;用产酶36h所得酶液降解3%壳聚糖,酶解最适pH值为5.5—6.0,最适温度为50—55℃,10%的酶量完全降解3%的壳聚糖所需时间为30h.  相似文献   

4.
以虎眼万年青总皂苷得率为指标,采用正交试验法得出纤维素酶酶解最佳提取工艺.结果为酶解时料液比1:15、用酶量1%、酶解温度50℃、酶解时间30min,得率为0.67%.  相似文献   

5.
球孢白僵菌产几丁质酶液态发酵研究   总被引:1,自引:0,他引:1  
对球孢白僵菌Bb174产几丁质酶的液态发酵条件进行了试验研究。选择麸皮和蚕蛹粉作为培养基的基本成分。以不同比例的麸皮和蚕蛹粉进行试验,结果显示:麸皮和蚕蛹粉分别为5%,培养温度28℃,接种量10%,装液量10mL,起始pH6.0,添加0.3%NaNO3对产几丁质酶有利,最高酶活力为4664U/mL。  相似文献   

6.
以高温预处理稻草秸秆为原料,分别进行纤维素酶、木聚糖酶单因子和正交试验,探索秸秆适宜工艺条件。结果表明:在1∶8的酶解固液比条件下,添加纤维素酶15 FPU/g底物和木聚糖酶200 IU/g底物,于50℃温度下酶解48 h,将获得82.13%的还原糖得率。说明以纤维素酶及木聚糖酶处理稻草秸秆是可行方法之一。  相似文献   

7.
优化纤维素降解菌株CY10产酶条件,为该菌株在纤维素的生物降解方面提供实验依据.通过单因素试验对CY10菌株产酶条件进行优化,依据纤维素酶(CMCase)和滤纸糖化酶(FPase)活力大小确定最适培养时间、初始p H、碳源、氮源、温度、接种量及装液量等.结果表明:菌株CY10产酶的最适初始p H为6.5,碳源为麦麸(2.0%),氮源为酵母粉(0.5%),在30℃培养42 h;该菌株所产CMC酶和滤纸酶的酶活性最适温度为45℃,最适p H为6.5.在此条件下,CY10菌株的CMCase和FPase活力分别达到21.26 U/m L和23.57 U/m L.为此,菌株CY10在纤维素的生物降解方面具有较好的应用前景.  相似文献   

8.
通过N^+离子束诱变斜卧青霉A10,经透明圈法初筛,摇瓶发酵复筛,测定酶活,获得一株高产纤维素酶的突变菌株LA17,酶活达5.48IU/ml.与出发株相比,其产酶能力提高44.20%.  相似文献   

9.
紫外线复合Nd:YAG激光对Bacillus sp.产脂肪酶的诱变   总被引:3,自引:0,他引:3  
以Bacillus sp.为出发菌株,经过紫外线诱变,筛选出较稳定的菌株UV-4,其产脂肪酶活力较出发菌株提高了28%;UV-4经过YAG激光诱变处理,得到变株Y-1,其产酶能力在此基础上又提高了41%,且产酶水平较为稳定。  相似文献   

10.
对自行筛选的碱性果胶酶生产菌培养条件进行了优化。考查了碳源、氮源浓度和接种量对发酵产酶的影响。经单因素实验和正交试验研究,该菌株最佳产酶条件组合为:果胶添加量0.3%、NaNO。添加量0.6%、接种量6%。在此条件下,碱陛果胶酶酶活力达到6079U/mL。  相似文献   

11.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

12.
《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.  相似文献   

13.
王菲 《华章》2007,(12):273-273
Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.  相似文献   

14.
裴水妹 《华章》2007,(11):196
Sister Carrie is one of the most controversial characters in American literature.Thought as a "fallen woman" firstly,she was defined as a "new woman" by some critics later. However, by digging into the motivaton behind the whole process of Carrie's "success", the relationship between Carrie and her creator (the author), the social conditions of then American, it can be found that Carrie has never been free-standing on her thought and she has never found her real-sdf even after becoming a famous actress. In a society dominated by mass consumerism Carrie is only an adherent of her own desires. She also is a representative of all those country girls flooded into cities, a symbol and a sacrifice of the urbanization of America in a time countryside was overcome by cities.  相似文献   

15.
16.
1.IntroductionOne-cyclecontrolmethod,whichwasproposedaboutonedecadeago[1],hasbecomeanattractivemethodinspecialfieldssuchaspowerfactorcorrection[2-6],switchingamplifiers[7,8],etc.Themainideaofthiscontrollerisbasedonintegrationofdiodevoltageinone-cycleandforcesittobeexactlyequaltothereferencevalue.Themainadvantageofthiscontrollerisitsrealtimeabilitytorejectthevariationofinputvoltage[1].Despitethisgreatability,ithasnogoodperformancesinrejectingofloaddisturbanceandfollowingreferencecommands.Espec…  相似文献   

17.
Today, Sunday morning, I sit thinking and typing at my computer. My thoughts are in my homeland, India and my body is in China. The body is here and mind there for a good reason. Mr. Hu is president and I am resident and both are in each other's countries (what a difference the absence of one small letter makes!). Mr. Hu will return in a few days and I will stay on longer.  相似文献   

18.
STORIES     
正A One day a pig went to the stable(马棚)to see his good friend,an oldhorse,and was going to stay there forthenight.Night came and it was time forsleep.The pig went into the straw heap(稻草堆)and lay comfortably.A longtime passed,but the horse was stillstanding there and did not move.Thepig asked the horse why he did not go tosleep."Standing like this is the begin-ning ofsleeping,"answered the horse.  相似文献   

19.
正1.Any fool can count the seeds in an apple.Only God can count all the apples in one seed.2.Happy families are all alike;every unhappy family is unhappy in its own way.3.Memories are the key not to the past,but to the future.4.Sometimes in the dark you see what you want to see.5.There is a great difference between  相似文献   

20.
正Scope and Policy The Journal of Chongqing University-English Edition(JCQU-E)is a peerreviewed journal publishing research articles in multiple disciplines of science and technology,covering civil and environmental engineering,chemical engineering,biotechnology,mechanical and electrical engineering,computer and data processing,mining,metallurgy,materials science,and interdisciplines thereof.  相似文献   

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