共查询到20条相似文献,搜索用时 15 毫秒
1.
K. Poornima M. Cariappa K. Asha H. P. Kedilaya M. Nandini 《Indian journal of clinical biochemistry : IJCB》2003,18(2):197-205
An adequate antioxidant reserve which is usually present in a vegetarian diet is associated with higher life expectancy. On
the other hand habitual consumption of fish is associated with lower risk of cardiovascular diseases which is attributed to
the polyunsaturated fatty acids (PUFA) present in it. However the PUFA are readily susceptible to oxidation leading to the
formation of free radicals which is known to be involved in the causation of several diseases.
The oxidant-antioxidant status of 23 vegetarians and 22 fish eaters was studied by determining the plasma lipid peroxides
measured as malondialdehyde (MDA) and the antioxidants, viz. glutathione (GSH), ascorbic acid, ceruloplasmin and uric acid.
Results show that the ascorbic acid values were significantly higher in vegetarians. In addition, MDA correlated negatively
with ascorbic acid only in vegetarians. However, correlation of MDA with glutathione showed a significant negative correlation
only in fish eaters. There was no statistically significant difference in the MDA, GSH, ceruloplasmin and uric acid levels.
The mechanism behind these findings are not clear and needs to be explored. 相似文献
2.
D. C. Sharma Raj Kiran Vijaywantee Ramnath Krishna Khushiani P. P. Singh 《Indian journal of clinical biochemistry : IJCB》1994,9(2):100-102
Blood haemoglobin, serum iron, iron binding capacity, transferrin saturation and ferritin levels were determined in two groups of mothers as well as their cords—strict vegetarians (lactovegetarians) and non-vegetarians (omnivores), closely comparable in age, weight, parity and gestation period but differing in their diet and food habits. All these parameters, except total iron binding capacity, were found to be significantly lower in vegetarian mothers and their cords as compared to nonvegetarian mothers and their cords, respectively, despite receiving supplemental iron for six months. Further, there was a greater incidence of anemia and iron deficiency in mothers consuming only vegetarian diet. Moreover, a significant correlation existed between mother's ferritin to cord ferritin confirming that maternal iron deficiency does affect neonatal iron status. All these observations suggest that strict vegetarian mothers as well as their newborns have a greater incidence and risk of anemia and iron deficiency. 相似文献
3.
R. K. Singh A. A. Mahdi A. K. Singh S. K. Bansal J. Wu S. Zhou F. Halberg 《Indian journal of clinical biochemistry : IJCB》1992,7(2):185-192
Circadian periodicity of human circulating total (T), high-density lipoprotein (HDL) and low-density lipoprotein (LDL + VLDL; briefly LDL) cholesterol (C) was studied in 30 clinically healthy, diurnally active, norturnally resting young volunteers divided into two groups of 15 each (10 men; 5 women) taking vegetarian and non-vegetarian diets. A marked circadian variation in T-C, HDL-C and LDL-C was recorded in all volunteers irrespective of the diety habits. However, total mean values and the acrophase differed between the two groups. Lower MESOR of studied variables for the vegetarians and higher MESOR for the omnivores in both the sexes exhibited endogeneous rhythmic changes as well as lower values of cholesterol components in vegetarians as compared to omnivores. Fasting unmasks a presumably endogeneous change around a lower MESOR with a smaller circadian amplitude both of HDL and LDL cholecterol metabolism. The recognition that the human circulating cholesterol components oscillate physiologically, in the peripheral blood with a shift of acrophase according to a circadian rhtyhm in two dietry schedules may prove to be of significance in the clinical interpretation of the laboratory result under tropical conditions. 相似文献
4.
R. B. Bhogade A. N. Suryakar N. G. Joshi R. Y. Patil 《Indian journal of clinical biochemistry : IJCB》2008,23(3):233-237
Hemodialysis represents a chronic stress status for its recipients. Many hypotheses state that this may be associated with
oxidative stress. Thus, there may be deficiency of antioxidants like erythrocytic superoxide dismutase, catalase, vitamin
E or increased generation of free radicals like superoxide anions. A study was carried out to investigate oxidant and antioxidant
status in chronic renal failure patients undergoing hemodialysis and effect of vitamin E supplementation on these two status.
Blood samples were collected from patients before and after hemodialysis and from controls. The samples were analyzed for
quantitation of MDA as index of lipid peroxide, nitric oxide, vitamin E, vitamin C and enzymatic antioxidants namely erythrocyte
SOD and catalase. As compared to controls, the levels of serum MDA were significantly increased and activities of erythrocyte
SOD and catalase, levels of serum nitric oxide, serum vitamin E and plasma vitamin C were significantly decreased both before
and after hemodialysis. The efficiency of vitamin E therapy in hemodialysis patients was assessed by re-evaluating oxidant
and antioxidant status of same patients after supplementation of vitamin E. Vitamin E supplementation caused decrease in serum
MDA and increase in levels of serum nitric oxide, vitamin E, vitamin C and activities of erythrocytic SOD and catalase. Our
results suggest the presence of oxidative stress and the possible preventive role of vitamin E therapy in hemodialysis patients. 相似文献
5.
S. Kumari S. Panda M. Mangaraj M. K. Mandal P. C. Mahapatra 《Indian journal of clinical biochemistry : IJCB》2008,23(2):158-162
Hyperglycemia and dyslipidemia in diabetes mellitus induce increased lipid peroxidation and peroxyl radical formation, an
important mechanism in genesis of microangiopathy. We took up a study on oxidative stress, measured by plasma MDA and antioxidant
vitamin status in type − 2 DM patients with and without retinopathy and compared them with a control non-diabetic group. Lipid
peroxidation marker MDA was significantly elevated (p < 0.001) in both the diabetic groups whereas, serum vitamin E and vitamin
C registered a significant fall (p<0.001) as compared to controls. Our correlation study revealed a significant positive association
between plasma MDA with both fasting and 2hr post prandial plasma glucose (r=0.81, p < 0.001, r=0.92, p <0.001) suggesting
the role of hyperglycemia in free radical production. Plasma MDA also depicted significant positive relation (p< 0.001) with
all lipid parameters except serum HDLc pointing the role of dyslipidemia towards lipid peroxidation. Plasma MDA level was
also found to be negatively correlated with both the vitamins (p<0.001, p<0.001) in the study group explaining their protective
consumption in the oxidative process prevailing in diabetic retinopathy. 相似文献
6.
Hem Lata G. K. Ahuja A. P. S. Narang Lily Walia 《Indian journal of clinical biochemistry : IJCB》2004,19(2):1-4
Stress is one of the basic factors in the aetiology of a number of diseases such as atherosclerosis, coronary heart disease,
aging, liver disease etc. Hence this work was designed to study the effect of stress in the form of forced immobilisation
stress on lipid peroxidation and lipid profile in rabbits. The study was conducted in 25 healthy rabbits of either sex. Rabbits
were subjected to forced immobilisation for two hours everyday for consecutive seven days. Blood samples were collected on
day 1, 3 and 7 after immobilisation period. Malondialdehyde (MDA), a marker of lipid peroxidation was estimated by TBA assay
method. Lipid profile was analysed by multichannel autoanalyser. There was statistically significant increase in MDA, total
cholesterol, LDL, VLDL and triglycerides with immobilisation. These findings indicate that stress in the form of forced immobilisation
increases lipid peroxidation and alter lipid profile, which may be responsible for pathophysiology of various diseases. 相似文献
7.
D. Sreeramulu B. A. Ramalakshmi N. Balakrishna N. Raghuramulu 《Indian journal of clinical biochemistry : IJCB》2004,19(1):79-82
The objective of the present study was to evaluate the changes in serum dehydroepiandrosterone (DHEA) and lipid peroxide levels
during ageing in human subjects. Random blood samples were collected from a total of 128 apparently normal human volunteers
of both sexes, whose age ranged between 21–70 years. The subjects were divided into groups of a decade years of age difference.
Serum levels of dehydroepiandrosterone (DHEA), lipid peroxides as malondialdehyde (MDA), and insulin were analysed in all
the subjects recruited in the study. In the present study there was decrease in serum DHEA levels with age (11.30 to 7.99
ng/ml). However the differences were significant only after 50 years up to 70 years. Serum MDA levels of these subjects were
higher (3.91 to 4.74 ng/ml) as compared to the values reported earlier (2.64 to 3.94 ng/ml). The serum MDA levels also showed
an increasing trend with age but the increase was significant only in the 40–50 years age group and values plateaud off at
later ages. The MDA and DHEA levels in women in general were lower than in men and this could be due to female hormones, which
are known to protect lipid against peroxidation. There was a significant negative correlation between age and DHEA (r=−0.311
P<0.05) and positive correction between MDA and age (r=+0.405 P<0.01). No significant differences were seen in serum insulin,
albumin and total protein levels. These preliminary findings support the possible utility of DHEA and MDA as markers for chronological
ageing. 相似文献
8.
Benedicta D’Souza Vivian D’Souza S. Sowmya G. Seema C. N. Hemalatha M. Anu G. Sajana 《Indian journal of clinical biochemistry : IJCB》2008,23(3):218-222
The study was conducted to find out the extent of lipid peroxidation and antioxidant status in ischemic stroke patients (ISPs)
with and without diabetes. Malondialdehyde (MDA) was studied as a marker of lipid peroxidation. Glutathione (GSH), uric acid
and ceruloplasmin were estimated to study the antioxidant potential of ISPs. Significantly higher levels of MDA were found
in both the groups of ISPs and the increase in MDA was more in ISPs without diabetes. GSH levels were decreased significantly
in both the groups of ISPs and maximum decline was found in ISPs with diabetes. Uric acid levels were significantly increased
in both the groups of ISPs. Ceruloplasmin levels were increased significantly in ISPs without diabetes, whereas its levels
were slightly decreased in ISPs with diabetes. A negative correlation was found between MDA and the antioxidants GSH, uric
acid and ceruloplasmin in ISPs with diabetes. This study suggests that there is an association between ischemic stroke and
increased oxidative stress and the antioxidant potential is impaired in both the groups of ISPs with and without diabetes. 相似文献
9.
Anjali Sharma Simmi Kharb Vineeta Nirmal Gulati 《Indian journal of clinical biochemistry : IJCB》1998,13(2):123-125
The present study revealed significantly lower levels of serum magnesium in patients with preterm labour without any apparent
cause as compared to normal term pregnant women in labour. The values were also lower in women with low socioeconomic status,
older age and consuming vegetarian diet. Hence, estimation of serum magnesium levels in pregnancy may be a useful parameter
in preterm labour. 相似文献
10.
N. Unni C. Sumithra R. Lakshman Lakshmi N. Leela Menon K. N. Subhakumari V. S. Sheejamol 《Indian journal of clinical biochemistry : IJCB》2015,30(2):161-166
PCOS is a heterogeneous endocrine disorder with diverse clinical presentation. Oxidative stress plays a key role in the pathophysiology of this disease. Serumhigh sensitive C-reactive protein (hsCRP), a marker of chronic low grade inflammation, is indicative of future development of cardiovascular disease. Our aim is to evaluate the oxidant status and hsCRP levels in PCOS. The study involved 61 cases and 61 controls in the age group of 18–40 years diagnosed with PCOS. Erythrocyte malondialdehyde (MDA), superoxide dismutase (SOD), serum hsCRP, gonadotrophins, thyroid stimulating hormone, prolactin, glycemic status and lipid profile were estimated. Erythrocyte MDA (p < 0.001), SOD (p = 0.007) and serum hsCRP (p < 0.001) were significantly elevated in PCOS patients than controls. Oxidative stress is present in women with PCOS along with elevated hsCRP. 相似文献
11.
Krishna Mohan Surapneni V. S. Chandrasada Gopan 《Indian journal of clinical biochemistry : IJCB》2008,23(1):41-44
The changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin
E (non enzymatic antioxidant parameters) and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase
(GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) activity were estimated in patients with rheumatoid
arthritis. This work was undertaken to assess oxidative stress and anti oxidant status in patients with rheumatoid arthritis.
It was observed that there was a significant increase in erythrocyte MDA levels, activities of SOD, GPX, plasma GST and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalse activity in
patients with rheumatoid arthritis when compared to controls. The results of our study suggests higher oxygen free radical
production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and Catalase activity, support to the oxidative
stress in rheumatoid arthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response
to increased oxidative stress. 相似文献
12.
Lipid peroxidation (MDA) was studied spectrophotometrically in relation to lipidemic status and atherogenic index in IHD and IHD risk subjects. It was found to be significantly elevated compared to control subjects (P<0.005) in both the groups, showing a linear correlation with cholesterolemic status. The values were found to run parallel to atherogenic index. In 10% cases with borderline atherogenic index the lipid peroxidation was found to be highly elevated. It is speculated that it may have a future role as a marker for IHD risk specially in premature group. 相似文献
13.
N. P. Suryawanshi A. K. Bhutey A. N. Nagdeote A. A. Jadhav G. S. Manoorkar 《Indian journal of clinical biochemistry : IJCB》2006,21(1):126-130
The study was designed to find out the correlation between lipid peroxidation, lipoprotein levels to severity and complication
of diabetes mellitus. Degree of lipid peroxidation was measured in terms of malondialdehyde (MDA) along with lipid profile
and blood glucose in diabetes mellitus. It is categorised into insulin dependent diabetes mellitus (IDDM), non insulin dependent
diabetes mellitus (NIDDM) and diabetes mellitus(DM) with complication.
Total 112 known diabetic patients and 52 non-diabetic controls were studied. These patients were grouped as per the concentration
of fasting blood glucose level i.e. controlled, poorly controlled, and uncontrolled group. There are significant increase
in the lipid peroxide (MDA) and lipid profile except HDL cholesterol which is decreased, has been found in all groups as compared
to controls In NIDDM group lipid peroxidation was markedly increased than IDDM group and it was higher in DM with complications.
Other finding observed was that the level of lipid peroxide increased as per the increase in concentration of blood glucose.
The increase lipid peroxidation in the hyperglycemic condition may be explained, as the superoxide dismutase enzyme which
is antioxidant becomes inactive due the formation of superoxide radical within the cell. Maximum lipid peroxidation leads
to the damage of the tissue and organs which results into complication in diabetic patients. High levels of total cholesterol
appear due to increased cholesterol synthesis. The triglyceride levels changes according to the glycemic, control. The increase
may be due to overproduction of VLDL-TG.
It is concluded that good metabolic control of hyperglycemia will prevent in alteration in peroxidation and the lipid metabolism,
which may help in good prognosis and preventing manifestation of vascular and secondary complication in diabetes mellitus. 相似文献
14.
Sarama Saha Chandan Sarkar Subhash Chandra Biswas R. Karim 《Indian journal of clinical biochemistry : IJCB》2008,23(3):262-266
Abnormal lipid profile is often found in women with Polycystic Ovary Syndrome. To assess the impact of abnormal lipid profile
on atherosclerosis in young Polycystic Ovary Syndrome women, carotid intima-media thickness as judged by B-mode ultrasonography
were done in 30 young (18–35 yrs) Polycystic Ovary Syndrome women and in similarly age-matched 30 apparently healthy controls.
Compared to controls, young Polycystic Ovary Syndrome women had significantly elevated serum total cholesterol, triglyceride
and LDL-C levels and carotid intima-media thickness. HDL-C level did not differ significantly between two groups of women.
In Polycystic Ovary Syndrome women carotid intima-media thickness was positively correlated with serum total cholesterol,
triglyceride and LDL-C and negatively correlated with serum HDL-C. Our study suggests that even young Polycystic Ovary Syndrome
women are prone to atherosclerosis from early age. 相似文献
15.
Maternal and fetal indicators of oxidative stress during intrauterine growth retardation (IUGR) 总被引:1,自引:0,他引:1
Ullas Kamath Guruprasad Rao Shobha U Kamath Lavanya Rai 《Indian journal of clinical biochemistry : IJCB》2006,21(1):111-115
The present study demonstrates the possibility of increased lipid peroxidation and protein oxidation in both maternal and
fetal erythrocytes as markers of oxygen radical activity during intrauterine growth retardation. The erythrocyte MDA levels
were significantly elevated in mothers of IUGR babies when compared to controls (p<0.01). The endogenous protein damage due
to oxidative stress was significantly higher in IUGR mothers when compared to controls (p<0.05). Similarly the proteolytic
activity in erythrocyte lysates against oxidatively damaged hemoglobin was significantly increased in mothers of IUGR babies
compared to controls (p<0.001).
In fetuses born with IUGR, both lipid peroxidation and proteolytic activity were significantly increased when compared to
normal newborns (p<0.01).
The result of this study indicates that oxidative stress was induced both in IUGR babies and their mothers which is manifested
as increased lipid peroxidation and protein oxidant damage. 相似文献
16.
Nosratollah Zarghami Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):86-91
It has been proposed that oxidative stress plays an important role in male infertility. The aims of this study were to compare
seminal plasma levels of 15-F2t-isoprostane (8-iso-PGF2α), malondialdehyde (MDA), and total (sum of free and bound) homocysteine
(tHcy) from normozoospermic vs. asthenozoospermic men, and to examine the relationships between tHcy and lipid peroxidation
products. The study was a case-control study with a simple random sampling. The case group was consisted of 15 asthenozoospermic
males. This group was compared with 15 normozoospermic men. Seminal plasma levels of 15-F2α-isoprostane and tHcy were measured
using commercially available enzyme immunoassay (EIA) kits. MDA levels were determined by the thiobarbituric acid (TBA) assay.
The Mann-Whitney U test was used to compare two groups. Coefficients of correlation were calculated using Spearman’s correlation
analysis. All hypothesis tests were two-tailed with statistical significance assessed at the p value <0.05 level. MDA levels
were higher in asthenozoospermic subjects than in control subjects (0.72±0.06 μM vs. 0.40±0.06 μM; p<0.05). No differences
were seen in 15-F2α-isoprostane levels in asthenozoospermic subjects and controls (65.00±3.20 pg/ml vs. 58.17±4.12 pg/ml;
p>0.05). Interestingly, tHcy levels were to be slightly higher in asthenozoospermic subjects than in controls (6.18±1.17 μM
vs. 4.8±0.52 μM). Sperm motility was inversely correlated with seminal plasma 15-F2α-isoprostane and MDA levels, respectively
(p<0.05). In summary, seminal plasma levels of 15-F2α-isoprostane and tHcy showed no significant difference between normozoospermic
and asthenozoospermic men. Sperm motility was not correlated with seminal plasma levels of tHcy. No relationship was found
between tHcy and lipid peroxidation. 相似文献
17.
Upma Garg Anju Jain Parul Singla Sarita Beri Rajiv Garg Arvind Saili 《Indian journal of clinical biochemistry : IJCB》2012,27(2):196-199
Retinopathy of prematurity (ROP) is a major cause of blindness in children. Free radicals are implicated in the development of this retinopathy. We studied the role of free radicals in ROP and enrolled 60 preterm neonates at 30–32 weeks age. Thirty neonates predisposed to development of ROP, were placed in study group and 30 normal neonates in control group. Malondialdehyde and antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) were measured in blood spectrophotometrically. Both the groups were followed-up to 40–42 weeks age. Serum MDA levels, erythrocyte SOD and plasma GPX were significantly high in study group at 30–32 weeks as compared to control group. At follow up visit significant increase in MDA level and decrease in SOD and GPX level among the study group was seen. This disturbance in equilibrium of oxidant and antioxidant status initiates an inflammatory process in retinal tissue leading to development of ROP. 相似文献
18.
P. Sabitha Kannan Vaidyanathan D. M. Vasudevan Prakash Kamath 《Indian journal of clinical biochemistry : IJCB》2009,24(1):76-81
In this study, we compared the lipid profile and antioxidant enzymes of normal and diabetic subjects consuming two different
types of oil as cooking medium. 70 normal, healthy subjects were taken as controls and 70 subjects with Type 2 diabetes were
recruited in patient group. Each group was further subdivided into two subgroups of 35 subjects each, consuming coconut oil
and sunflower oil respectively as cooking medium. Samples of blood were collected and analyzed for serum total cholesterol,
triacylglycerols, and cholesterol in lipoprotein fractions. Total glutathione and glutathione peroxidase were measured in
erythrocytes and superoxide dismutase in serum. Triacylglycerols, LDL and VLDL cholesterol levels were high in the diabetic
subjects compared to the controls. Total glutathione and glutathione peroxidase values showed significant decrease in diabetic
subjects as compared to the controls, while superoxide dismutase values showed significant difference between coconut oil
consuming groups. Though lipid profile parameters and oxidative stress were high in Type 2 diabetic subjects compared to controls,
no pronounced changes for these parameters were observed between the subgroups (coconut oil vs. sunflower oil). 相似文献
19.
Jyoti Titus Suresh Chari Madhur Gupta Nitin Parekh 《Indian journal of clinical biochemistry : IJCB》2004,19(2):168-172
The role of oxidant damage to red cells in sickle cell anaemia has been of interest in recent years. Although, available reports
suggest that sickle cell erythrocytes are susceptible to endogenous free radical mediated oxidant damage there remains discrepancy
in the status of antioxidant enzymes and antioxidant vitamins in these patients. In view of this, 107 cases of sickle cell
anaemia (36 ‘SS’ and 71 ‘AS’ pattern—as confirmed by haemoglobin electrophoresis) were subjected to analysis of malondialdehyde,
ascorbic acid, superoxide dismutase and albumin. The results were compared with 54 age and sex matched healthy controls. The
results indicate a marked increase in lipid peroxidation and superoxide dismutase levels in both ‘SS’ and ‘AS’ types of sickle
cell anaemia as compared to controls. Although no difference was observed in the levels of albumin in these groups the levels
of ascorbic acid were significantly depleted in sickle cell anaemia patients. The results are indicative of enhanced lipid
peroxidation along with imbalance in the pro-oxidant and antioxidant status in patients of sickle cell anaemia. 相似文献
20.
The present study was carried out to assess the biochemical profile and outcome of pregnancy and study the adverse consequences
if any, among normal and high risk pregnant women. The study group included 182 normal and 168 high risk cases attending to
private and Government Hospitals in Bangalore. The high risk groups were: Pregnancy induced hypertension (PIH), gestational
diabetes mellitus (GDM), Adolescents and anemic cases. Lipid peroxidation was enhanced in PIH and GDM groups (5.56 nmol/ml
and 3.98 nmol/ml) MDA values as compared to other groups. Vitamin E levels were significantly (p< 0.05) lower in PIH group
(0.38 mg/dl) as compared to other groups. Caesarean as a mode of delivery indicating more number of complications were higher
among GDM (61.9%) followed by PIH group. Incidences of low birth weight were observed more in PIH group. The study revealed
occurrence of oxidative stress and adverse outcome among high risk pregnancy groups. 相似文献