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1.
Seasonal dynamics of total phenolics (TP),extractable condensed tannins (ECT),protein-bound condensed tannins (PBCT),fiber-bound condensed tannins (FBCT),total condensed tannins (TCT),and protein precipitation capacity (PPC) in young,mature and senescent branehlets of Casuarina equisetifolia were studied at Chishan Forestry Center of Dongshan County,Fujian Province,China.In addition,nitrogen contents of branehlets at the different developmental stages were also determined.The contents of TP and ECT,and PPC in young branchlets were significantly higher than those in mature and senescent branehlets through the season.However,PBCT contents were significantly higher in senescent branchlets than those in young and mature branchlets;FBCT fluctuated with season.Young branchlets had the highest N content,which decreased during branch maturity and senescence.The highest contents of TP and the lowest contents of TCT and N in young and mature branchlets were observed in summer.There was a significant negative correlation between TP and N contents.In contrast,TCT contents were positively correlated to N contents.Nutrient resorption during senescence and high TCT:N ratios in senescent branchlets are the important nutrient conservation strategies for C.equisetifolia.  相似文献   

2.
Tannins from Canarium album with potent antioxidant activity   总被引：1，自引：0，他引：1  
The contents of total phenolics and extractable condensed tannins in the leaves,twigs and stem bark of Canarium album were determined.The structural heterogeneity of condensed tannins from stem bark was characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) analyses.The results show the predominance of signals representative of procyanidins and prodelphinidins.In addition,epicatechin and epigallocatechin polymers with galloylated procyanidin or prodelphinidin were also observed.The tannins were screened for their potential antioxidant activities using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) model systems.Tannins extracted from leaves,twigs and stem bark all showed a very good DPPH radical scavenging activity and ferric reducing power.  相似文献   

3.
杂交水稻离体叶片衰老与蛋白质代谢的关系   总被引：3，自引：0，他引：3  
杂交水稻离体叶片衰老时，叶片中的叶绿素和蛋白质含量下降．游离氨基酸含量增加．氨肽酶和内肽酶的活性显著上升，说明在衰老叶片中氨肽醇和内肽酶的作用是蛋白质降解的主要原因．  相似文献   

4.
A rice pse(t) (premature senescence, tentatively) mutant line, was isolated from 4500 independent T-DNA inserted transgenic lines. The symptoms of premature senescence appeared more severely than those of the control plants (Zhonghua 11,japonica) at the last development stage. To characterize the mutant and provide basic information on the candidate genes by mapping to a physical region of 220-kb, experiments were carried out in two phytotrons under controlled temperature of 24 ℃ and28 ℃, respectively. The content of chlorophyll, soluble protein and MDA (malondialdehyde), net photosynthesis, the antioxidant enzyme activities of SOD (superoxide dismuase) (EC 1.15.1.1) and POD (peroxidase) (EC 1.11.1.7) and the peptidase activities of leaves were measured from top to bottom according to the leaf positions at the flowering stage. Compared with the control plant,the mutant showed the following characteristics: (1) Higher net photosynthesis rate (Pn) appeared in the 1 st and 2nd leaves, contents of chlorophyll and soluble protein were also higher in the 1 st leaf; (2) The activities of SOD, POD and peptidase were higher according to the leaf position from top to bottom; (3) The symptom of premature senescence was accelerated in the mutant at 28℃treatment. The MDA content and the SOD and POD activities between the 24 ℃ and 28 ℃ treatment mutants were not significantly different. Content of chlorophyll and soluble protein of leaves mutant decreased rapidly at 28 ℃ treatment. The results show that pse(t) is sensitive to high temperature. The probable function of PSE(T) is discussed.  相似文献   

5.
Although the biochemical dissection of parasitoid-host interactions is becoming well characterized, the molecular knowledge concerning them is minimal. In order to understand the molecular bases of the host immune response to parasitoid attack, we explored the response of Papilio xuthus parasitized by the endoparasitic wasp Pteromalus puparum using proteomic approach. By examining the differential expression of plasma proteins in the parasitized and unparasitized host pupae by two-dimensional (2D) electrophoresis, 16 proteins were found to vary in relation to parasitization compared with unparasitized control samples. All of them were submitted to identification by mass spectrometry coupled with a database search. The modulated proteins were found to fall into the following functional groups: humoral or cellular immunity, detoxification, energy metabolism, and others. This study contributes insights into the molecular mechanism of the relationships between parasitoids and their host insects. Project supported by the National Basic Research Program (973) of China (No. 2006CB102005), the National Natural Science Foundation of China (Nos. 30571251 and 30170626), the Program for New Century Excellent Talents in University of the Ministry of Education of China (No. NCET-05-0513), and the Innovation Research Team Program of the Ministry of Education of China (No. IRT0535)  相似文献   

6.
Leaf senescence is often caused by water deficit and the chimeric gene PSA612-1PT is an auto-regulated gene delaying leaf senescence. Using in vitro leaf discs culture system, the changes of contents of chlorophylls, carotenoids, soluble protein and thiobarbituric acid reactive substance （TBARS） and antioxidant enzymes activities were investigated during leaf senescence of PSA612-1PT modified gerbera induced by osmotic stress compared with the control plant （wild type）. Leaf discs were incubated in 20%, 40% （w/v） polyethylene glycol （PEG） 6 000 nutrient solution for 20 h under continuous light [ 130 μmol/（m^2·s）]. The results showed that the contents of chlorophylls, carotenoids and soluble protein were decreased by osmotic stress with the decrease being more pronounced at 40% PEG, but that, at the same PEG concentration the decrease in the transgenic plants was significantly lower than that in the control plant. The activities of superoxide dismutase （SOD）, catalases （CAT）, ascorbate peroxidase （APX）, guaiacol peroxidase （GPX） and dehydroascorbate reductase （DHAR） were stimulated by PEG treatment. However, the increases were higher in PSA612-IPT transgenic plants than in the control plants, particularly at 40% PEG treatment. Lipid peroxidation （TBARS content） was increased by PEG treatment with the increase being much lower in transgenic plant than in the control plant. It could be concluded that the increases in the activities ofantioxidant enzymes including SOD, CAT, APX, GPX and DHAR were responsible for the delay of leaf senescence induced by osmotic stress.  相似文献   

7.
The definition of J-integral of interfacial crack was introduced. The three-point bending tests were carried out to obtain the critical loading values when the interfacial crack initiation occurred between coatings and substrates. The finite element analysis (FEA) was adopted to analyze the stress distribution in the specimens and compute the J-integral of the interfacial crack between LX88A coating and Chinese Q345 steel. The results showed that the average value of critical J-integral is 0.70 N/m, which can be taken as the fracture parameter to evaluate the interfacial fracture behavior for the three-point bending specimens of LX88A coating/Q345 steel system. Supported by Tianjin Natural Science Foundation (No.08JCYBJC09100), New Teacher Research Fund for the Doctoral Program of Higher Education of China (No.20070056096) and New Century Outstanding Talented Person Plan of China. XU Lianyong, born in 1975, male, Dr.  相似文献   

8.
Objective: To investigate the factors favoring a positive prognosis for advanced primary peritoneal carcinoma (PPC). Methods: Twenty-four cases meeting the criteria for PPC were analyzed retrospectively for the clinicopathologic profiles. Immunohistochemistry was used to determine the expressions of p53, Top2α, Ki-67 and Her-2/neu. Then all these clinicopathological factors and molecular markers were correlated with the prognosis. Results: There were 15 cases of primary peritoneal serous papillary carcinoma (PPSPC), 6 cases of mixed epithelial carcinoma (MEC) and 3 cases of malignant mixed Mullerian tumor (MMMT). All patients underwent cytoreductive surgery with optimal debulking achieved in 3 cases. Among those receiving first-line chemotherapy, 13 patients received the TP regimen (paclitaxel-cisplatin or carboplatin) and 7 patients received the PAC regimen (cisplatin-doxorubicin-cyclophosphamide). The median overall survival of all patients was 42 months, while the breakdown for survival time for patients with PPSPC, MMT and MEC was 44, 13 and 19 months, respectively. The expressions of p53, Top2a and Ki-67 were all demonstrated in 11 cases respectively. None showed the expression of Her-2/neu. There were significant differences in the median survival between patients with PPSPC and those with MMMT (44 months vs 13 months, P<0.05), also between patients receiving TP combination and those receiving the PAC regimen (75 months vs 28 months, P<0.05). Another significant difference in the median progression-free survival (PFS) was identified between patients with positive p53 immunostaining and those with negative p53 immunostaining (15 months vs 47 months, P<0.05), whereas age, menopausal status, residual tumor size and the other molecular factors did not significantly impact survival. Conclusion: Patients with PPC should be treated with a comprehensive management plan including appropriate cytoreductive surgery and responsive chemotherapy. Overestimating an optimal debulking surgery may not benefit survival. The pathologic subtype, chemotherapy regimen and p53 overexpression were significant prognostic factors.  相似文献   

9.
The effects of CO2 enrichment on the growth and glucosinolate (GS) concentrations in the bolting stem of Chinese kale (Brassica alboglabra L.) treated with three nitrogen (N) concentrations (5, 10, and 20 mmol/L) were investigated. Height, stem thickness, and dry weights of the total aerial parts, bolting stems, and roots, as well as the root to shoot ratio, significantly increased as CO2 concentration was elevated from 350 to 800 μl/L at each N concentration. In the edible part of the bolting stem, 11 individual GSs were identified, including 7 aliphatic and 4 indolyl GSs. GS concentration was affected by the elevated CO2 concentration, N concentration, and CO2×N interaction. At 5 and 10 mmol N/L, the concentrations of aliphatic GSs and total GSs significantly increased, whereas those of indolyl GSs were not affected, by elevated atmospheric CO2. However, at 20 mmol N/L, elevated CO2 had no significant effects on the concentrations of total GSs and total indolyl GSs, but the concentrations of total aliphatic GSs significantly increased. Moreover, the bolting stem carbon (C) content increased, whereas the N and sulfur (S) contents decreased under elevated CO2 concentration in the three N treatments, resulting in changes in the C/N and N/S ratios. Also the C/N ratio is not a reliable predictor of change of GS concentration, while the changes in N and S contents and the N/S ratio at the elevated CO2 concentration may influence the GS concentration in Chinese kale bolting stems. The results demonstrate that high nitrogen supply is beneficial for the growth of Chinese kale, but not for the GS concentration in bolting stems, under elevated CO2 condition. Project (No. 2007CB109305) supported by the National Basic Research Program (973) of China  相似文献   

10.
Recently there has been an increasing interest in applying random walk based methods to recommender systems. We employ a Gaussian random field to model the top-N recommendation task as a semi-supervised learning problem, taking into account the degree of each node on the user-item bipartite graph, and induce an effective absorbing random walk （ARW） algorithm for the top-N recommendation task. Our random walk approach directly generates the top-N recommendations for individuals, rather than predicting the ratings of the recommendations. Experimental results on the two real data sets show that our random walk algorithm significantly outperforms the state-of-the-art random walk based personalized ranking algorithm as well as the popular item-based collaborative filtering method.  相似文献   

11.
The rice water weevil, Lissorhoptrus oryzophilus Kuschel, has two generations in southern Zhejiang, China. To determine oogenesis in first-generation females （summer females） and its relations to temperature, females were collected from a rice field in early and mid-July and reared on young rice plants at 28, 31 and 34 ℃ in the laboratory. Percentage of females having oocytes, number of oocytes of different stages （stage-Ⅰ, from early previtellogenesis to middle vitellogenesis; stage-Ⅱ, late vitellogenesis; and matute-oocyte stage）, and length of ovarioles were determined every 10 d of feeding. At each temperature, oogenesis took place in over 40% of females after 20-40 d of feeding, but only 0.0-3.3 stage-Ⅰ, 0.0-0.8 stage-Ⅱ and 0.0-1.1 mature oocytes were observed at each observation date. Temperature had significant effect on number of stage-Ⅰ oocytes but not on number of stage-Ⅱ and mature oocytes in early July females; temperature had no significant effect on number of oocytes of either stage in mid-July females. Conclusively, in southern Zhejiang, summer L. oryzophilus females have great potential to become reproductive on rice, but their oogenesis activity is very low, with the overall procedures little affected by temperature.  相似文献   

12.
Background: Sevoflurane and propofol are effective cardioprotective anaesthetic agents, though the cardioprotection of propofol has not been shown in humans. Their roles and underlying mechanisms in anesthetic postconditioning are unclear.Mitochondrial permeability transition pore (MPTP) opening is a major cause of ischemia-reperfusion injury. Here we investigated sevoflurane- and propofol-induced postconditioning and their relationship with MPTP. Methods: Isolated perfused rat hearts were exposed to 40 min of ischemia followed by 1 h of reperfusion. During the first 15 min of reperfusion, hearts were treated with either control buffer (CTRL group) or buffer containing 20 μmol/L atractyloside (ATR group), 3% (v/v) sevoflurane (SPC group),50 μmol/L propofol (PPC group), or the combination of atractyloside with respective anesthetics (SPC ATR and PPC ATR groups). Infarct size was determined by dividing the total necrotic area of the left ventricle by the total left ventricular slice area (percent necrotic area). Results: Hearts treated with sevoflurane or propofol showed significantly better recovery of coronary flow,end-diastolic pressures, left ventricular developed pressure and derivatives compared with controls. Sevoflurane resulted in more protective alteration of hemodynamics at most time point of reperfusion than propofol. These improvements were paralleled with the reduction of lactate dehydrogenase release and the decrease of infarct size (SPC vs CTRL: (17.48±2.70)% vs (48.47±6.03)%,P＜0.05; PPC vs CTRL: (35.60±2.10)% vs (48.47±6.03)%, P＜0.05). SPC group had less infarct size than PPC group (SPC vs PPC:(17.48±2.70)% vs (35.60±2.10)%, P＜0.05). Atractyloside coadministration attenuated or completely blocked the cardioprotective effect of postconditioning of sevoflurane and propofol. Conclusion: Postconditioning of sevoflurane and propofol has cardioprotective effect against ischemia-reperfusion injury of heart, which is associated with inhibition of MPTP opening. Compared to propofol, sevoflurane provides superior protection of functional recovery and infarct size.  相似文献   

13.
研究了23种红树植物（15种真红树植物,8种半红树植物）叶片衰老过程中氮（N）、磷（P）内吸收率.结果表明：（1）真红树植物与半红树植物相比,其叶片营养元素含量相对较低（Nmass=17.3mg/g;Pmass=1.4mg/g）,N、P含量随叶片衰老而减少;（2）大部分红树植物叶片氮磷比小于31,说明红树植物存在氮限制;（3）真红树植物氮内吸收率平均为63.6%,远高于半红树植物（52.2%）以及一般常绿植物（47.0%）,但真红树植物磷内吸收（53.4%）和半红树植物（49.4%）不存在显著的差异.因此,真红树植物拥有更高的氮保存能力,使其更适应于在缺氮环境下生存.  相似文献   

14.
A known species, Physarum melleum, was found fruiting on living leaves of Dendrobium candidum, which was collected in China in 2004. Its morphological characters were revealed by light microscopy (LM), environmental scanning electron microscopy (ESEM) and scanning electron microscopy (SEM). Character variations were distinguished by its olive-yellow peridium and its always thinner capillitium containing globulose granular material between the large calcareous nodes. The calcium carbonate granules, deposited on stalks, peridium and hypothallus as well as within stalks, were globose and smooth. Project (No. 2001BA744C) supported by the 10th Five-year National Key Technologies R & D Program, China  相似文献   

15.
采用定期取样杀青烘干化验分析的方法，探索在不同N，P，K营养条件下白肋烟生长发育过程中的糖、氮、烟碱等化学物质变化规律，结果表明：总糖、还原糖随生长期推进呈增加趋势，而总氮、蛋白质呈下降趋势；随施N量增加各生长期的总氮、蛋白质、烟碱增加，而旺长、成熟期总糖和伸根、旺长前期、成熟期的还原糖下降；随施P量增加各生长期的总氮和伸根、旺长前期、成熟期的蛋白质、烟碱增加，而旺长期的总糖、旺长前期的还原糖和旺长后期的蛋白质、烟碱下降；随施K量增加各生长期的总氮、烟碱、蛋白质下降；N，K对总糖和还原糖，N，P对烟碱、总氛、蛋白质影响较大．  相似文献   

16.
The increasingly serious problem of acid rain is leading to increased potassium (K) loss from soils, and in our field investigation, we found that even congenerically relative Mosla species show different tolerance to K-deficiency. A hydroponic study was conducted on the growth of two Mosla species and their morphological, physiological and stoichiometric traits in response to limited (0.35 mmol K/L), normal (3.25 mmol K/L) and excessive (6.50 mmol K/L) K concentrations. Mosla hangchowensis is an endangered plant, whereas Mosla dianthera a widespread weed. In the case of M. hangchowensis, in comparison with normal K concentration, K-limitation induced a significant reduction in net photosynthetic rate (P n), soluble protein content, and superoxide dismutase (SOD) activity, but an increase in malondialdehyde (MDA) concentration. However, leaf mass ratio (LMR) and root mass ratio (RMR) were changed little by K-limitation. In contrast, for M. dianthera, K-limitation had little effect on P n, soluble protein content, SOD activity, and MDA concentration, but increased LMR and RMR. Critical values of N (nitrogen): K and K:P (phosphorus) ratios in the shoots indicated that limitation in acquiring K occurred under K-limited conditions for M. hangchowensis but not for M. dianthera. We found that low K content in natural habitats was a restrictive factor in the growth and distribution of M. hangchowensis, and soil K-deficiency caused by acid rain worsened the situation of M. hangchowensis, while M. dianthera could well acclimate to the increasing K-deficiency. We suggest that controlling the acid rain and applying K fertilizers may be an effective way to rescue the endangered M. hangchowensis. Project supported by the National Natural Science Foundation of China (No. 30570113) and the Research Fund for the Doctoral Program of Higher Education (No. 20060335008), China  相似文献   

17.
选取79名闽南地区幼儿(年龄4-6岁)作为研究被试,采用"匹配伪装实验"调查他们对普通话、英语、闽南语三种语言的态度。研究表明:闽南地区幼儿对普通话和英语的评价明显高于闽南语,幼儿对普通话掌握水平高,对闽南语掌握水平低,闽南语的发展存在危机;不同类型的幼儿对普通话评价差异不大,对英语和闽南语的评价存在显著差异。幼儿语言态度受到学校语言教育、家庭经济水平、社会地位等方面的影响。幼儿对闽南语存在态度与行为上的矛盾。  相似文献   

18.
Time-management skills are acknowledged to be important but there has been little actual research on this topic with students. In this study we examined the scores obtained from 293 first-year students of psychology on a British version of an American time-management scale. The students were divided into three age groups: traditional-entry students - aged less than 21 years (N = 172); borderline mature students - aged 21–25 years (N = 50) and older mature students - aged more than 25 years (N = 71). Our analyses indicated (i) that women students in general reported significantly greater time-management skills than did men students, and (ii) that our older mature students reported significantly better time-management skills than did the other two groups. Academic performance, however, was only modestly predicted by age and scores on one component of the time-management scale.  相似文献   

19.
The goal of this study was to explore links among shyness, receptive vocabulary, and indices of socioemotional functioning in a sample of young children in mainland urban China. In particular, we sought to examine the potential moderating role of receptive language in these relations. Participants were N = 360 young children (200 boys, 160 girls, Mage = 4.72 years, SD = 0.63) attending preschool/kindergartens in Shanghai, People’s Republic of China. Multisource assessments were employed, including maternal ratings (child shyness), standardized tests (receptive vocabulary), child self-reports collected during individual interviews (loneliness), teacher ratings (child internalizing problems), and peer nominations (peer liking). Results indicated that receptive vocabulary moderated (buffering effect) the relations between shyness and several indices of socioemotional functioning. Results are discussed in terms of the implications of language skills for early school adjustment of young shy Chinese children.  相似文献   

20.
Previously we have designed and implemented new image browsing facilities to support effective offiine image contents on mobile devices with limited capabilities： low bandwidth, small display, and slow processing. In this letter, we fulfill the automatic production of cartoon contents fitting small-screen display, and introduce a clustering method useful for various types of cartoon images as a prerequisite stage for preserving semantic meaning. The usage of neural networks is to properly cut the various forms of pages. Texture information that is useful for grayscale image segmentation gives us a good clue for page layout analysis using the multilayer perceptron （MLP） based x-y recursive algorithm. We also automatically frame the segment MLP using agglomerative segmentation. Our experimental results show that the combined approaches yield good results of segmentation for several cartoons.  相似文献   

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