共查询到20条相似文献，搜索用时 187 毫秒
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萤火虫荧光素酶基因cDNA真核表达载体pGL2可在原核细胞中高效表达。该载体中含有SV40早期启动子及荧光素酶基因cDNA5'端总长共758bp的DNA碱基序列，经计算机分析发现，在SV40早期启动子中含有SV40基因组HindⅢB片断中一段长为49bp的DNA序列，且荧光素酶基因cDNA编码区的5'端含有一个原核细胞基因表达调控区相似序列。将pGL2中的荧光素酶基因cDNA（Luc）克隆入真核表达载体pED中，构建成pED-Luc。该载体经表达测定后，只在真核细胞中表达，在原核细胞不中表达。这说明荧光素酶基因cDNA编码区的5'端的原核细胞基因表达调控区相似序列不具表达有活性的荧光 素酶的功能。经DNA序列对比研究，认为pGL2含有的SV40基因组Hind ⅢB片段5123bp-5171bp长49bp的DNA序列可能就是Hind ⅢB片段的原核增强子功能的决定序列。 相似文献
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基因线路是生命体对自身生命过程控制的动态调控系统。在工程化的设计原理的指导下,人工基因线路是对天然基因调控线路进行简单化处理和重新编程,以及引入自然界不存在的人造法则。人工基因线路由遗传开关、生物振荡器、逻辑门等组成,以执行诸多调控功能。多种多样的人工基因线路设计与构建,不仅极大地促进了人们对生命调控基本规律的认识,也进一步丰富了人们对天然的生物系统进行改造、再创的手段,并为医药健康、农业环境和工业发酵等领域的实际需求提供了全新解决方案。虽然在过去20年里,人们在人工基因线路领域取得了丰硕的研究成果,但是细胞体内蕴含着的众多复杂生化反应和信号传导途径,为设计和组装具有更加高级功能的基因线路带来了挑战。相应地,如何实现微小细胞内复杂基因线路的可预测设计组装,如何保障基因线路在复杂的体内外环境下发挥稳健的功能,将成为未来几年人工基因线路研究的关键核心问题和势必克服的重大挑战。 相似文献
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中国已获得了一批具有知识产权的新基因、新表达系统，生物工程药物进入了创制阶段，建立了一系列关键平台技术、动、植物转基因技术已经成熟，具备了大规模基因测序和生物芯片、生物信息的研究条件。 相似文献
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人类基因组今天已经扩大到对单个个人的基因组的描绘。与此同时,人类对其他动物的基因组也有了较多的发现,包括破译它们的基因组和发现各种功能基因。这不仅对生物保护、生物多样性有帮助,而且有助于人类从动物获得启示,为自己的健康、治病和长寿服务。 相似文献
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生命科学研究的进展日新月异。科技信息的交流和成果的沟通是开展科学研究的支柱之一。本文概述了分子生物学、植物细胞分化以及基因导入等三个相互渗透的学科进展的动态,以启发我们如何根据自己的国情合理布局研究课题,有效使用科研经费。分子生物学是研究组成生物本身的重要物质:核酸、蛋白质和酶的结构和功能,合成和代谢,表达和调控以及它们之间的相互作用,以此来揭示生命的本质,是生命科学研究的前沿和核心。细胞分化是生物发育的依据,它始终作为生命之谜为世人瞩目。归根结底是基因表达的调控,外源基因的导入则是研究遗传工程的重要手段,只有外源基因被受体整合后,才有可能实现其获得性状的表达和遗传。 相似文献
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Dengue fever is one of the major health problems in India. Interaction with specific receptor(s) at the cell surface is one
of the first events in the pathogenesis of Dengue virus. However, relatively little is known about these receptors. Cellular
receptors in human monocytes and mouse neural cells are main target for the viral infection. The envelope protein of the virus
(E-protein) plays important role in attachment of virus to target cells and their interaction with cellular receptors. The
modulation of receptor gene(s) and/or protein(s) can be used as a method for interfering with virus entry and can thus become
a new method for disease prevention. The receptors can be purified by affinity chromatography using E-protein as ligand. It
has been reported that addition of highly sulfated heparan sulfate prevents E-protein binding to target cells suggesting that
heparan sulfate is utilized by dengue envelope protein to bind to target cells. 相似文献
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《Information processing & management》1999,35(2):181-191
The automatic text summary concerns the language industries. This work proposes a system automatically and directly transforming a source text into a reduced target text. The system deals exclusively with scientific and technical texts. It is based on the identification of specific expressions allowing an evaluation of the relevance of the sentence concerned, which can then be selected for the elaboration of the summary. The procedure consists in attributing a score to each sentence of the text and then eliminating those having the lowest scores. To produce the RAFI system (automatic summary based on indicative fragments), we resorted to the linguistic means of discourse analysis and the computing capacity of data processing instruments. This system could be adapted to Internet. 相似文献
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Boolean logic performs a logical operation on one or more logic input and produces a single logic output. Here, we describe a microfluidic DNA computing processor performing Boolean logic operations for gene expression analysis and gene drug synthesis. Multiple cancer-related genes were used as input molecules. Their expression levels were identified by interacting with the computing related DNA strands, which were designed according to the sequences of cancer-related genes and the suicide gene. When all the expressions of the cancer-related genes fit in with the diagnostic criteria, positive diagnosis would be confirmed and then a complete suicide gene (gene drug) could be synthesized as an output molecule. Microfluidic chip was employed as an effective platform to realize the computing process by integrating multistep biochemical reactions involving hybridization, displacement, denaturalization, and ligation. By combining the specific design of the computing related molecules and the integrated functions of the microfluidics, the microfluidic DNA computing processor is able to analyze the multiple gene expressions simultaneously and realize the corresponding gene drug synthesis with simplicity and fast speed, which demonstrates the potential of this platform for DNA computing in biomedical applications. 相似文献
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基因芯片又称DNA微阵列,该技术将大量的核苷酸探针以点阵列的方式排布于固相支持物上,与荧光标记的DNA样品杂交,通过激光共聚焦等方法对样品DNA进行定量检测。基因芯片技术具有自动化,高通量的特点,是基因表达谱研究的一种重要手段,在农业,医学,药物学等方面也有着广阔的应用前景。如何对基因芯片实验中所产生的大量数据进行有效的生物学分析,已成为芯片研究中的一个热点。基因芯片数据分析的流程通常包括了表达谱数据的获取与标准化,差异表达基因的筛选,基因的功能性分类及生物学意义的分析。 相似文献
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[目的/意义]随着信息的爆炸性增长,科研人员摄取信息的途径越来越多,但对知识的控制能力越来越弱。因此,不论信息如何变化,科研人员需要抓住知识基因的进化过程(科技创新路径)寻找有价值知识,极大提高知识利用效率。[方法/过程]在前期充分辨析科技文献知识基因的基础上,根据科技文献知识基因的特征将科技文献知识基因分为通用知识基因与特定知识基因两种类型。提出利用知识基因表征科技创新路径的节点,根据知识流动的特点,将科技创新路径划分为基于知识基因扩散与知识基因流动的科技创新路径。通过计算知识基因之间遗传或变异的关系,嫁接引用路径构建出知识基因表达的科技创新路径,并通过知识基因间的连线实现可视化表达。[结果/结论]通过阿尔茨海默病领域实验证明,基于知识基因表达的科技创新路径能够从科技文献核心知识价值传承与发展的角度直观地展示出科技创新发展与演化过程。 相似文献
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BackgroundMolecular brain therapies require the development of molecular switches to control gene expression in a limited and regulated manner in time and space. Light-switchable gene systems allow precise control of gene expression with an enhanced spatio-temporal resolution compared to chemical inducers. In this work, we adapted the existing light-switchable Light-On system into a lentiviral platform, which consists of two modules: (i) one for the expression of the blue light-switchable trans-activator GAVPO and (ii) a second module containing an inducible-UAS promoter (UAS) modulated by a light-activated GAVPO.ResultsIn the HEK293-T cell line transfected with this lentiviral plasmids system, the expression of the reporter mCherry increased between 4 to 5 fold after light induction. A time expression analysis after light induction during 24 h revealed that mRNA levels continuously increased up to 9 h, while protein levels increased throughout the experiment. Finally, transduction of cultured rat hippocampal neurons with this dual Light-On lentiviral system showed that CDNF, a potential therapeutic trophic factor, was induced only in cells exposed to blue light.ConclusionsIn conclusion, the optimized lentiviral platform of the Light-On system provides an efficient way to control gene expression in neurons, suggesting that this platform could potentially be used in biomedical and neuroscience research, and eventually in brain therapies for neurodegenerative diseases.How to cite: Zárate RV, Arancibia D, Fernández A, et al. Optimization of the Light-On system in a lentiviral platform to a light-controlled expression of genes in neurons. Electron J Biotechnol 2021;51. https://doi.org/10.1016/j.ejbt.2021.03.006 相似文献