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1.
通过研究乙烯对盐胁迫中玉米种子活力、幼苗生长及SOD活性的影响,发现外源乙烯可提高盐胁迫下两品种玉米种子活力和SOD活性,但幼苗鲜重和高度低于对照,表明盐胁迫中乙烯通过提高玉米种子活力和SOD活性,且使生长受到抑制来增加玉米的抗盐性.  相似文献   

2.
钙浸种对辣木种子萌发和幼苗生长的影响   总被引:3,自引:0,他引:3  
研究了不同浓度Ca^2+溶液浸种对辣木种子萌发和幼苗生长的影响。结果表明,Ca^2+浓度为10mmol·L^-1最有利于种子萌发和幼苗生长,其种子萌发率、发芽势、简化活力指数、根长、根数、苗高、茎粗、叶片数等均显著高于对照,随着浓度升高作用减弱,20mmol·L^-1Ca^2+的处理对种子萌发率和发芽势有明显的抑制作用。Ca^2+浸种虽然增加了平均萌发天数,但显著提高了出苗率。  相似文献   

3.
本以玉米品种大黄和晴3为材料,研究NaCl胁迫过程中玉米幼苗胚芽鞘的谷胱甘肽还原酶活性及可溶性蛋白质的变化.玉米幼苗分别用700nmol/L的NaCl及蒸馏水处理(对照)5d后,发现NaCl处理过的幼苗的存活率比对照低,存活幼苗的生长也受到影响,表明此胁迫处理已影响了玉米幼苗的生长,而使存活率降低.在用此胁迫强度的NaCl盐处理的过程中抗氧化酶GR在处理初期活性有所上升,而后期下降,而且NaCl处理过的酶液中的可溶性蛋白质的含量比对照低.以上结果表明,在胁迫初期玉米通过GR活性的增加来清除活性氧以适应盐胁迫;随着胁迫强度的加大,酶液中的可溶性蛋白质的含量逐渐减少,使酶液中GR的含量减少,导致酶活性降低.通过研究玉米幼苗盐胁迫中的GR活性及可溶性蛋白质的变化,证实GR在盐胁迫中所起的作用,从而揭示抗氧化系统与植物抗逆性的关系.  相似文献   

4.
镉胁迫对玉米萌发的影响   总被引:1,自引:0,他引:1  
以7个不同品种的玉米为材料,通过水培实验研究了不同浓度的Cd2+ (0.5 mg/L、1 mg/L、5mg/L、10 mg/L)对玉米种子萌发的影响.结果表明,低浓度Cd2+ (0.5 mg/L、1mg/L)对所有品种玉米种子的萌发表现为促进作用,而高浓度Cd2+ (5 mg/L、10mg/L)表现为抑制作用.在7个玉米品种中,迪卡656和长城1142在所有浓度的Cd2+胁迫下的萌发率较好.  相似文献   

5.
混合盐碱胁迫对玉米种子萌发的影响   总被引:14,自引:0,他引:14  
分别用不同浓度的NaCl、Na2CO3以及二者的混合溶液处理玉米种子,模拟中性盐、酸性盐和混合盐碱对种子萌发及一些生理指标的影响.结果表明,用单纯盐、碱、混合盐碱溶液处理的玉米种子,对其萌发均有抑制作用,种子的发芽率、发芽指数、活力指数下降,根冠比降低,丙二醛含量升高,α-淀粉酶活性降低.危害程度随着处理浓度的增大而加剧.3种处理对种子萌发的抑制程度为Na2CO3>混合盐碱>NaCl.  相似文献   

6.
吴诗光 《商丘师专学报》2007,23(12):109-112
以两个大豆品种(周豆11号和豫豆24号)为材料,研究了不同浓度Ni^2+处理对大豆种子萌发和幼苗生长中膜脂过氧化水平、细胞膜透性及保护酶(SOD、POD、CAT)活性的影响.结果表明,(1)Ni^2+浸种处理对大豆种子萌发具有低浓度下的激活效应和高浓度下的抑制效应;(2)细胞内丙二醛(MDA)含量和细胞膜透性在低浓度Ni^2+处理下略有降低,尔后随着镍浓度的提高其质膜透性和MDA含量逐渐增大,体内保护酶系统中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化表现为先升后降的趋势.不同大豆品种对NP的反应存在霉鼻与抗氢化系统的整体活力有关.  相似文献   

7.
钙和青霉素对老化棉花种子幼苗生长的影响   总被引:2,自引:0,他引:2  
研究了Ca^2 和青霉素处理液对老化棉花种子幼苗生长的影响,结果表明:0.03%的青霉素和0.07%的Ca^2 能显著提高其过氧化物酶(POD)活性,幼苗长度,幼苗根系活力,但对幼苗叶片中叶绿素含量的影响不显著。  相似文献   

8.
玉米幼苗干旱过程中钙的效应   总被引:3,自引:0,他引:3  
CaCl2处理明显地提高了玉米幼苗的抗旱性,而且能使热激诱导抗旱性过程中的抗氧化酶GR的活性、热稳定蛋白质及游离脯氨酸的含量升高,而降低质膜相对透性,表明CaCl2可能是通过GR、热稳定蛋白质和脯氨酸的抗氧化作用保护细胞膜免受破坏或破坏程度受到削弱而提高植物抗旱性。  相似文献   

9.
玉米种子经较高浓度(1~5mmol/L)的水杨酸预处理后,萌发都受到抑制,发芽种子中的可溶性蛋白质含量和可溶性糖含量及过氧化物酶(POD)活性降低,淀粉酶活性升高;若用较低浓度(0.01-0.1mmol/L)的水杨酸处理.则促进种子的萌发,降低淀粉酶活性,可增加过氧化物酶活性、可溶性糖和可溶性蛋白质含量.  相似文献   

10.
以两个大豆品种(周豆11号和豫豆24号)为材料,研究了不同浓度Ni2 处理对大豆种子萌发和幼苗生长中膜脂过氧化水平、细胞膜透性及保护酶(SOD、POD、CAT)活性的影响.结果表明,(1)Ni2 浸种处理对大豆种子萌发具有低浓度下的激活效应和高浓度下的抑制效应;(2)细胞内丙二醛(MDA)含量和细胞膜透性在低浓度Ni2 处理下略有降低,尔后随着镍浓度的提高其质膜透性和MDA含量逐渐增大,体内保护酶系统中超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性的变化表现为先升后降的趋势.不同大豆品种对Ni2 的反应存在差异,与抗氧化系统的整体活力有关.  相似文献   

11.
Low temperature stress during germination and early seedling growth is an important constraint of global production of maize. The effects of seed priming with 0.25%, 0.50%, and 0.75% (w/v) chitosan solutions at 15 ℃ on the growth and physiological changes were investigated using two maize (Zea mays L.) inbred lines, HuangC (chilling-tolerant) and Mo17 (chilling-sensitive). While seed priming with chitosan had no significant effect on germination percentage under low temperature stress, it enhanced germination index, reduced the mean germination time (MGT), and increased shoot height, root length, and shoot and root dry weights in both maize lines. The decline of malondialdehyde (MDA) content and relative permeability of the plasma membrane and the increase of the concentrations of soluble sugars and proline, peroxidase (POD) activity, and catalase (CAT) activity were detected both in the chilling-sensitive and chilling-tolerant maize seedlings after priming with the three concentrations of chitosan. HuangC was less sensitive to responding to different concentrations of chitosan. Priming with 0.50% chitosan for about 60~64 h seemed to have the best effects. Thus, it suggests that seed priming with chitosan may improve the speed of germination of maize seed and benefit for seedling growth under low temperature stress.  相似文献   

12.
Objective:To evaluate the effect of chitosan(CH) and hydrogen peroxide(H2O2) seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology,the H2O2 presence,the catalase(CAT) activity,and the protein quantity.Methods:Seven groups of ten seeds for each maize variety were treated with CH(2%(20 g/L) and 0.2%(2 g/L)) or H2O2(8 mmol/L) by coating,sprinkling,or both.Germination and seedling growth were measured.One month after germination,the presence of H2O2 in seedlings in the coated seed treatments was evaluated.Protein content and CAT activity were determined under all treatments.Results:H2O2 seed coating enhanced the germination rate and increased seedling and stem length in the quality protein maize(QPM) variety.Seedlings had a higher emergence velocity under this treatment in both varieties.CH and H2O2 sprinklings did not have an effect on seedling phenology.Exogenous application of H2O2 promoted an increase of endogenous H2O2.CH and H2O2 seedling sprinkling increased the protein content in both maize varieties,while there was no significant effect on the CAT activity of treated seeds and seedlings.Conclusions:CH and H2O2 enhance some phenological and biochemical features of maize depending on their method of application.  相似文献   

13.
以不同浓度骆驼蓬提取液浸泡燕麦种子,通过室内培养研究对种子活力、幼苗生长、呼吸速率及淀粉酶、脂肪酶和转氨酶活力的影响.结果表明,骆驼蓬提取物明显降低种子活力,抑制幼苗生长和呼吸速率及种子萌发过程中胚乳淀粉酶、脂肪酶和转氨酶活性,抑制作用随骆驼蓬提取液浓度提高而增强.  相似文献   

14.
铜对玉米种子萌发和生长的影响   总被引:5,自引:0,他引:5  
通过不同浓度的Cu~(2+)对玉米萌发和生长影响的研究,结果表明:当Cu~(2+)浓度为0.5~1mg/L时Cu~(2+)可以促进幼芽的生长和生物量的提高,而超过该浓度时则由促进逐渐转为抑制作用。Cu~(2+)对玉米幼苗根生长的影响大于对芽生长的影响。  相似文献   

15.
以玉米种子为试验材料,采用砂培法研究了水杨酸(SA)对铅(Pb2+)胁迫下玉米种子的萌发、叶绿素含量、MDA含量、POD活性、等方面的影响。结果表明:低浓度的SA≤80mg/L时,能促进铅胁迫下玉米幼苗的生长,表现为玉米种子的发芽率增大,幼苗根长的增加,叶绿素含量增大,MDA的含量降低,POD活性升高,等。当SA浓度为60mg/L~80mg/L,Pb2+≤1mg/L时,SA对Pb2+的解毒效应最明显,当SA浓度为100mg/L,Pb2+为8mg/L时,SA与Pb2+协同迫害幼苗的生长。  相似文献   

16.
A number of studies have shown the existence of cross-tolerance in plants, but the physiological mechanism is poorly understood. In this study, we used the germination of barley seeds as a system to investigate the cross-tolerance of low-temperature pretreatment to high-temperature stress and the possible involvement of reactive oxygen species (ROS) scavenging enzymes in the cross-tolerance. After pretreatment at 0 °C for different periods of time, barley seeds were germinated at 35 °C, and the content of malondialdehyde (MDA) and the activities of ROS scavenging enzymes were measured by a spectrophotometer analysis. The results showed that barley seed germinated very poorly at 35 °C, and this inhibitive effect could be overcome by pretreatment at 0 °C. The MDA content varied, depending on the temperature at which seeds germinated, while barley seeds pretreated at 0 °C did not change the MDA content. Compared with seeds germinated directly at 35 °C, the seeds pretreated first at 0 °C and then germinated at 35 °C had markedly increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR). The SOD and APX activities of seeds germinated at 35 °C after 0 °C-pretreatment were even substantially higher than those at 25 °C, and GR activity was similar to that at 25 °C, at which the highest germination performance of barley seeds was achieved. These results indicate that low-temperature pretreatment can markedly increase the tolerance of barley seed to high temperature during germination, this being related to the increase in ROS scavenging enzyme activity. This may provide a new method for increasing seed germination under stress environments, and may be an excellent model system for the study of cross-tolerance.  相似文献   

17.
种子是生命力的载体 ,其传热传质的研究已经从宏观迈向微观 ,从整体迈向细胞、原生质、细胞器、分子膜的水平 .用透射电镜对未干与干燥的白菜种子进行了子叶切片观察 ,发现干燥后的种子子叶细胞仅在细胞壁有皱褶 ,细胞器无明显变化 .种子发芽过程中酶活性检测表明 ,与未干种子相比 ,萌发 48h,经 45℃干燥 2 h的种子α-酶的相对活性下降了 5 .8% ;经 67℃干燥 2 h的种子α-酶的相对活性下降了 3 0 .1% .可见 ,干燥参数影响种子的微观结构与酶的活性 ,与种子干燥的临界安全温度相同 ,酶的活性是干燥温度、初含水率与加热时间的函数  相似文献   

18.
Coating seeds with water absorbent materials can improve their survival, especially for those planted in drought or barren areas. In this study, effects of five kinds of super absorbent polymers (SAPs) on seed germination and seedling growth of Caragana korshinskii under drought conditions were investigated. Our results showed that SAP coatings could significantly improve the percentage and energy of seed germination, as well as reduce the relative electrical conductivity (REC), proline, malondialdehyde (MDA), H2O2 content, and peroxidase (POD) activity during germination. These results implied that seeds could uptake moisture from SAP coatings to alleviate drought-induced oxidative stress and membrane damage, thus exhibiting a better vigor and germination performance. After coating C. korshinskii seeds with SAPs, more seedlings emerged and grew better. Under the combined influence of the water absorption capacity of SAP and other factors, the efficiencies of five SAP coatings are in the sequence D>E>B>A>C. The function of the SAP coating on promoting seedling survival was confirmed in Mu Us Sandy Land in Ordos, Inner Mongolia Autonomous Region, China. The average seedling number of SAP D-coated seeds increased twofold on that of naked seeds. Our results are expected to be helpful in understanding and utilizing SAP seed coatings in improving plant survival under drought conditions.  相似文献   

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