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【目的】:探讨沙参麦冬汤对混合烟雾所致慢性支气管炎模型大鼠肺脏组织的抗氧化能力及对肺脏组织结构的影响。【方法】:60只SD大鼠随机分为正常对照组、慢支模型组和沙参麦冬汤组。采用混合烟雾吸入法复制慢性支气管炎模型大鼠,烟熏期40d。正常对照组与慢支模型组灌胃生理盐水10mL/kg.d,沙参麦冬汤组灌服沙参麦冬汤10mL/kg.d(2g生药/mL),灌胃期30d。灌胃第30d,剖杀大鼠,测定右肺组织匀浆的超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢(CAT)的活力和丙二醛(MDA)的含量。左肺组织进行病理切片光镜观察。【结果】:沙参麦冬汤显著提高烟熏大鼠肺脏组织SOD活性和GSH-Px活性,对肺脏组织CAT活性无显著影响,可降低肺脏组织中MDA含量。正常对照组无明显病理形态学改变,慢支模型组肺泡腔不规则扩大,肺泡间隔破裂,融合成大泡,炎性细胞浸润,沙参麦冬汤组肺泡壁增厚,肺间质炎症细胞浸润。【结论】:沙参麦冬汤可以提高烟熏大鼠肺脏的抗氧化功能,减少氧化应激损伤。沙参麦冬汤能改善烟熏大鼠肺脏组织结构的损伤程度,但对肺组织和支气管的抗炎作用不明显。 相似文献
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观察大枣多糖对酒精肝大鼠组织抗氧化功能的影响。方法:选择SD大鼠50只,随机分成5组,每组10只。Ⅰ为空白对照组,Ⅱ为白酒对照组,Ⅲ为大枣多糖多糖低剂量组(4g/kg体重),Ⅳ为大枣多糖多糖中剂量组(8g/kg体重),Ⅴ为大枣多糖高剂量组(16g/kg体重)。除空白对照组外其余各组每天上午用56%白酒(8mL/kg)灌胃。六周后,处死各组大鼠,采取心、肝、脾、肺、肾组织0.5g,加生理盐水制成10%匀浆液,2500r/min离心(15min)取其上清液,测定SOD、CAT、GSH-Px的活性和MDA的含量。结果表明大枣多糖能提高酒精肝大鼠心、肝、脾、肺、肾的SOD、CAT、GSH-Px活力(P0.05或P0.01),同时降低其MDA含量(P0.05或P0.01)。说明大枣多糖对酒精肝大鼠心、肝、脾、肺、肾的损伤有一定的保护作用,这与大枣多糖能够清除氧自由基、增强抗脂质过氧化反应有关。 相似文献
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目的:研究滁菊总黄酮(TFCJ)抗大鼠局灶性脑缺血再灌注损伤(FCIRI)作用.方法:取120只雄性SD大鼠,随机分为6组,即假手术组、模型组、T FCJ高中低组和血塞通组,其中假手术组每日灌胃10 mL/kg0.5％ 羧甲基纤维素(CMC)溶液,手术仅分离血管;模型组每日灌胃10 mL/kg 0.5％CMC溶液,复制FCIRI模型;T FCJ高中低剂量组和血塞通组每日灌胃40 mg/kg、20 mg/kg、10 mg/kg T FCJ和25 mg/kg血塞通,用0.5％CM C溶液配制,复制FCIRI模型.以上全部大鼠每日灌胃1次,周期为7 d.使用神经评分系统、TTC染色和脑指数评估大鼠神经系统缺陷程度,SOD、GSH-Px、CAT和MDA评估氧化应激程度,H E染色用于评估脑组织病理变化.结果:取120只雄性S D大鼠,随机分为6组,假手术组每日灌胃10 mL/kg 0.5％ 羧甲基纤维素(CMC)溶液,手术仅分离血管;模型组每日灌胃10 mL/kg 0.5％CM C溶液,复制FCIRI模型;T FCJ高中低剂量组和血塞通组每日灌胃40 mg/kg、20 mg/kg、10 mg/kg TFCJ和25 mg/kg血塞通,用0.5％CMC溶液配制,复制FCIRI模型.以上全部大鼠每日灌胃1次,周期为7 d.使用神经评分系统、TTC染色和脑指数评估大鼠神经系统缺陷程度,SOD、GSH-Px、CAT和MDA评估氧化应激程度,HE染色用于评估脑组织病理变化.结论:TFCJ对大鼠RCIRI具有保护作用. 相似文献
4.
目的:观察康脑液对脑缺血再灌注大鼠脑含水量、梗死体积及神经功能评分的影响.方法:线栓法制备大鼠右侧大脑中动脉闭塞(Middle cerebral artery occlusion,MCAO)模型.120只雄性SD大鼠随机分为假手术组、模型组、康脑液大剂量组、康脑液中剂量组、康脑液低剂量组,大鼠缺血2h,分别再灌1d(即24h)、3d、7d,于大鼠苏醒后及再灌24h观察神经功能评分,再灌注24h后测大鼠脑梗死体积,再灌1d、3d、7d分别测各组大鼠脑含水量.结果:与模型组相比,康脑液治疗组大鼠脑梗死体积明显减小、脑含水量明显降低、大鼠神经功能评分显著改善(P＜0.05或P＜0.01).结论:康脑液对脑缺血再灌注大鼠神经细胞有保护作用,并能抑制脑水肿形成,减轻脑水肿程度,减轻神经功能损害. 相似文献
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山药多糖对糖尿病大鼠胰岛素及血小板数的影响 总被引：4，自引：0，他引：4
目的:观察山药多糖对四氧嘧啶诱导的糖尿病大鼠胰岛素以及血小板数的影响.方法:将四氧嘧啶糖尿病大鼠分成正常大鼠对照组、糖尿病模型对照组及治疗组,其中治疗组包括二甲双胍组和山药多糖小、中、大三个剂量组,二甲双胍组大鼠每日每只灌服二甲双胍剂量为0.8mg/kg,山药多糖小、中、大剂量组大鼠每日每只分别灌服50mg/kg、75mg/kg、100mg/kg的山药多糖精品.正常大鼠对照组、糖尿病模型大鼠对照组每日每只灌服等体积的生理盐水.灌服15d后进入实验程序的大鼠进行血糖测量.结果:与正常对照组比较,模型对照组大鼠血清胰岛素水平显著降低(P<0.01);山药多糖各剂量组的胰岛素水平无明显变化;与二甲双胍组比较,山药多糖各组胰岛素水平明显增高.山药多糖三个剂量组的血小板数与模型对照组比较有显著差异(P<0.01),与正常对照组比较无明显差异(P>0.05).结论:山药多糖对糖尿病大鼠的胰岛功能具有保护作用,能抑制血小板的异常激活和聚集. 相似文献
7.
目的：研究羊肚菌对大强度耐力运动大鼠肝组织自由基代谢与糖储备的影响.方法：24只雄性SD大鼠随机均分为安静对照组、运动对照组、运动加药组.后两组在动物实验跑台上进行8周大强度耐力运动,运动加药组每日按350 mg/kg体重的剂量灌胃羊肚菌粉混悬液,而前两组灌胃同等体积蒸馏水.8周后,分别检测各组大鼠肝组织中SOD、GSH-Px、CAT、MDA、血清酶GPT、血糖、肌糖原、肝糖原等指标.结果：大强度耐力运动引起大鼠肝组织抗氧化酶SOD活性下降（P<0.05）,GSH-Px、CAT活性下降（P<0.01）,脂质过氧化产物MDA含量和血清酶GPT活性上升（P<0.01）,血糖浓度与肌糖原含量降低（P<0.01）,肝糖原含量降低（P<0.05）;与运动对照组相比较,补充羊肚菌使大强度耐力运动大鼠肝组织抗氧化酶SOD、GSH-Px活性上升（P<0.05）,CAT活性上升（P<0.01）,脂质过氧化产物MDA含量和血清酶GPT活性下降（P<0.01）,血糖浓度、肌糖原含量上升（P<0.01）,肝糖原含量上升（P<0.05）.结论：补充羊肚... 相似文献
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实验结果表明:唐宫烟酒醒饮品属实际无毒级,30d灌服对大鼠无毒、无副作用;对灌服乙醇或静注尼古丁大鼠体内乙醇、尼古丁具加速代谢排泄作用;减轻乙醇对小鼠、大鼠、家鸽的中毒致死、麻醉及兴奋作用;缓解尼古丁对小鼠的毒性作用. 相似文献
10.
目的:本实验观察复心汤灌胃对大鼠心肌缺血模型的影响.方法:Wistar大鼠60只,雌雄各半,随机分成6组,每组10只.正常对照组:生理盐水灌胃;模型组:生理盐水灌胃;地奥心血康组:等效剂量地奥心血康灌胃(0.12g/kg/d);复心汤高剂量组:6倍等效剂量灌胃(81g/kg/d);复心汤中剂量组:3倍等效剂量灌胃(40.5g/kg/d);复心汤低剂量组:等效剂量灌胃(13.5g/kg/d).每日早晚各一次,连续7天.第8天除正常对照组外,其余各组于给药1小时后,用垂体后叶素(Pituitrinum,pit)制备心肌缺血模型.观察大鼠的心电图(ECG)、心肌组织乳酸脱氢酶(LDH)和肌酸激酶(CK)含量的改变、左室压力下降最大速率(?dp/dtmax)、左室舒张末压(LVEDP)的变化.结果:与模型组比较,复心汤各剂量组和地奥心血康组表现出显著对抗作用(P<0.05,P<0.01).与正常组比较,模型组大鼠CK及LDH活力显著升高,具有极显著性差异(P<0.001).结论:复心汤有治疗和预防心肌缺血的作用,对于心脏舒张功能的防治亦有明显的作用. 相似文献
11.
Betul Apaydin Yildirim Saban Kordali Kubra Asena Terim Kapakin Fatih Yildirim Esra Aktas Senocak Serdar Altun 《Journal of Zhejiang University. Science. B》2017,18(6):501-511
The aim of this study was to evaluate the possible therapeutic or protective effects of Helichrysum plicatum DC. subsp. plicatum ethanol extract (HPE) against gentamicin-induced nephrotoxicity. Thirty-six Sprague Dawley male rats weighing between 200 and 250 g were used as live material. They were formed into six groups containing 6 rats each and were allowed to adapt to laboratory conditions for 7 d. Group I: control, 5% DMSO intraperitoneal (i.p.); Group II: HPE 100 mg/(kg·d) i.p.; Group III: HPE 200 mg/(kg·d) i.p.; Group IV: gentamicin as 80 mg/(kg·d) i.p.; Group V: gentamicin as 80 mg/(kg·d) i.p.+HPE 100 mg/(kg·d) i.p.; and Group VI: gentamicin as 80 mg/(kg·d) i.p.+HPE 200 mg/(kg·d) i.p. for 8 d. Following treatment, serum, liver, and kidney tissues were used to assess blood urea nitrogen (BUN), creatinine, enzymatic and non-enzymatic antioxidants, and lipid peroxidation. Gentamicin significantly increased serum BUN, creatinin, and liver and kidney levels of malondialdehyde (MDA). It also decreased the activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD). Treatment with the HPE 100 mg/kg reversed gentamicin-induced alterations as evidenced by decreased serum BUN and creatinin, liver and kidney oxidant marker, and tubular necrosis as well as by an increase in antioxidant enzymes. It was found that HPE 200 mg/kg significantly increased liver and kidney tissue MDA levels in nephrotoxicity in rats. As a result, these findings support the proposition that HPE in 100 mg/kg dose demonstrates in the kidney and liver as free radicals and scavenger to prevent the toxic effects of gentamicin in both the biochemical and histopathology parameters. 相似文献
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INTRODUCTION Left ventricular hypertrophy has been thought to be the principal predicators of predisposing risk factor of cardiac morbidity and mortality (Devereux, 1995; Levy et al., 1990). The pathogenesis that mediates cardiac hypertrophy is poorly understood. Cardiachypertrophy can be induced by hemodynamic over-load, ischemic disease, neurohumoral factors and intrinsic defects in cardiac structural protein genes (Sadoshima and Izumo, 1997; Vikstrom and Lein-wand, 1996). Another in… 相似文献
13.
Effect of matrine and carvedilol on collagen and MMPs activity of hypertrophy myocardium induced by pressure overload 总被引：3，自引：0，他引：3
Zhang YJ Xiang MX San J Cheng G Wang SS 《Journal of Zhejiang University. Science. B》2006,7(3):245-250
INTRODUCTION Matrine, a monomer of traditional Chinese medi-cine, comes from leguminosae plants such as Kusheng, is quinoilizidine with four-loop and molecular formula of C15H24N20. Matrine has been proved to have anti-arrhythmia (Xu et al., 2004), anti-hypoxia and decreasing heart rate effects (Zhang et al., 1990a; 1990b) in many animal experiments, and has the role of inducing calmness (Luo et al., 2001) and lowering body temperature (Tao and Wan, 1992). Traditional Chinese medicin… 相似文献
14.
Jie Cui Zhi Li Ling-bo Qian Qin Gao Jue Wang Meng Xue Xiao-e Lou Iain C. Bruce Qiang Xia Hui-ping Wang 《Journal of Zhejiang University. Science. B》2013,14(6):487-495
Objective
To investigate the beneficial effect of bicyclol on rat hearts subjected to ischemia-reperfusion (IR) injuries and its possible mechanism.Methods
Male Sprague-Dawley rats were intragastrically administered with bicyclol (25, 50 or 100 mg/(kg·d)) for 3 d. Myocardial IR was produced by occlusion of the coronary artery for 1 h and reperfusion for 3 h. Left ventricular hemodynamics was continuously monitored. At the end of reperfusion, myocardial infarct was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining, and serum lactate dehydrogenase (LDH) level and myocardial superoxide dismutase (SOD) activity were determined by spectrophotometry. Isolated ventricular myocytes from adult rats were exposed to 60 min anoxia and 30 min reoxygenation to simulate IR injuries. After reperfusion, cell viability was determined with trypan blue; reactive oxygen species (ROS) and mitochondrial membrane potential of the cardiomyocytes were measured with the fluorescent probe. The mitochondrial permeability transition pore (mPTP) opening induced by Ca2+ (200 μmol/L) was measured with the absorbance at 520 nm in the isolated myocardial mitochondria.Results
Low dose of bicyclol (25 mg/(kg·d)) had no significant improving effect on all cardiac parameters, whereas pretreatment with high bicyclol markedly reduced the myocardial infarct and improved the left ventricular contractility in the myocardium exposed to IR (P<0.05). Medium dose of bicyclol (50 mg/(kg·d)) markedly improved the myocardial contractility, left ventricular myocyte viability, and SOD activity, as well decreased infarct size, serum LDH level, ROS production, and mitochondrial membrane potential in rat myocardium exposed to IR. The reduction of ventricular myocyte viability in IR group was inhibited by pretreatment with 50 and 100 mg/(kg·d) bicyclol (P<0.05 vs. IR), but not by 25 mg/(kg·d) bicyclol. The opening of mPTP evoked by Ca2+ was significantly inhibited by medium bicyclol.Conclusions
Bicyclol exerts cardioprotection against IR injury, at least, via reducing oxidative stress and its subsequent mPTP opening. 相似文献15.
丹参联合胞二磷胆碱治疗大鼠脑挫伤的机理研究 总被引：2，自引：0，他引：2
目的：探讨丹参与胞二磷胆碱联合应用治疗脑损伤的机制。方法：取成年wistar大鼠，以自由落体法致大鼠脑挫裂伤．采用721分光光度计检测伤后24h、7d和14d时．治疗组与对照组脑组织中SOD、MDA、GSH—PX和Ca^2＋含量。结果：治疗组SOD和GSH—PX活性提高．而且与模型组及对照组比较．SOD有显著性差异（P〈0．05）。治疗组脑组织Ca^2＋含量明显降低。结论：丹参在治疗脑损伤时，可以提高SOD活性，拮抗自由基和钙离子超载对脑的损伤。 相似文献
16.
Research on the protection effect of pioglitazone for non-alcoholic fatty liver disease （NAFLD） in rats 总被引：4，自引：0，他引：4
Objective： The prevalence of non-alcoholic fatty liver disease （NAFLD） has markedly increased. Insulin resistance has been implicated in the pathogenesis of NAFLD. This study was aimed at observing the relationship between insulin resistance and NAFLD, and evaluating the role of pioglitazone （PGZ） acting as insulin-sensitizing agents in the prevention and treatment of rat fatty liver induced by high fat feeding. Methods： The rats were separated randomly into 6 groups： model group Ⅰ were fed high fat diet for 8 weeks, PGZ prevention group were given PGZ 4 mg/（kg.d） simultaneously, while control group Ⅰ were fed normal food for 8 weeks; model group Ⅱ were fed high fat diet for 16 weeks, PGZ treatment group were given PGZ 4 mg/（kg.d） orally simultaneous with high fat diet for 8 weeks after high fat feeding for 8 weeks, control group Ⅱ were fed normal food for 16 weeks. The rats were sacrificed after 8 weeks and 16 weeks respectively. Liver weight, body weight, serum activities of alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, alkaline phosphatase （ALP）, tumor necrosis factor alpha （TNF-α）, fasting blood glucose （FBG）, fasting plasma insulin （FINS）, HOMA （homeostasis model assessment） insulin resistance index （HOMA-IR）, and the liver histology of rats of all groups were assayed. Results： After 8 weeks, the liver in model group Ⅰ showed typical steatosis, accompanied with mild to moderate lobular inflammatory cell infiltration, liver indexes and serum levels of ALT, AST, ALP, TNF-α were significantly increased （P〈0.05） compared with control group Ⅰ. Whereas, the degree of hepatic injury was attenuated in PGZ prevention group, liver indexes and serum levels of ALT, ALP were significantly decreased （P〈0.05） compared with model group Ⅰ. After 16 weeks, notable steatosis, and lobular inflammation were observed in model group Ⅱ rat liver, while the degree of hepatic injury was attenuated in the PGZ treatment group. Liver index, serum levels ofALT, AST, ALP, FINS and HOMA-IR were significantly increased （P〈0.05） in model group Ⅱ compared with control group Ⅱ. Whereas, in PGZ treatment group, serum levels of AST and FINS showed decreasing tendency, liver indexes, serum levels of ALT, ALP, TNF-α and HOMA-IR were significantly decreased compared with model group Ⅱ. Conclusion： Insulin resistance plays a role in the pathogenesis of NAFLD in rats. Pioglitazone can attenuate insulin resistance and biochemical and histological injury in high fat-induced fatty liver in rats. 相似文献
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贾文娟 《思茅师范高等专科学校学报》2013,(6):13-18
目的：通过实验观察甲醛对运动大鼠心肌组织抗氧化系统的影响及服用刺五加后的作用,探讨甲醛对运动大鼠的心肌组织毒性及刺五加的保护作用。方法：采用人为模拟不同程度甲醛染毒环境进行动式染毒,甲醛剂量分别为0.8 mg/m3,2.4 mg/m3。每天染毒30 min,每周6次,连续4周,刺五加的灌胃剂量为104.17 mg/kg,测定运动大鼠心肌组织的超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-PX）、过氧化氢酶（CAT）的活力和丙二醛（MDA）的含量。结果：染毒组与对照组相比,2.4 mg/m3剂量染毒运动组大鼠心肌组织 CAT、GSH-PX、SOD活性明显低于对照组（P〈0.01）,MDA含量明显高于对照组（P〈0.01）。服药染毒运动组与染毒运动组相比,0.8mg/m3剂量染毒服药组大鼠心肌组织CAT、GSH-PX、SOD活性明显高于染毒运动组（P〈0.01）,MDA含量明显低于染毒运动组（P〈0.01）。结论：甲醛可以降低染毒大鼠心肌组织抗氧化酶的活性,造成心肌组织氧化性损伤,而灌服中药刺五加可以有效缓解其毒性。 相似文献
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Weng Xiao-chuan Zhou Liang Fu Yin-yan Zhu Sheng-mei He Hui-liang Wu Jian 《浙江大学学报(A卷英文版)》2005,6(9):869-872
Objective: To compare the dose requirements of continuous infusion of rocuronium and atracurium throughout orthotopic liver
transplantation (OLT) in humans. Methods: Twenty male patients undergoing liver transplantation were randomly assigned to
two comparable groups of 10 patients each to receive a continuous infusion of rocuronium or atracurium under intravenous balanced
anesthesia. The response of adductor pollicis to train-of-four (TOF) stimulation of unlar nerve was monitored. The infusion
rates of rocuronium and atracurium were adjusted to maintain T1/Tc ratio of 2%–10%. The total dose of each drug given during
each of the three phases of OLT was recorded. Results: Rocuronium requirement, which were (0.468±0.167) mg/(kg·h) during the
paleohepatic phase, decreased significantly during the anhepatic phase to (0.303±0.134) mg/(kg·h) and returned to the initial
values at the neohepatic period ((0.429±0.130) mg/(kg·h)); whereas atracuruim requirements remained unchanged during orthotopic
liver transplantation. Conclusions: This study showed that the exclusion of the liver from the circulation results in the
significantly reduced requirement of rocuronium while the requirement of atracurium was not changed, which suggests that the
liver is of major importance in the clearance of rocuronium. A continuous infusion of atracurium with constant rate can provide
stable neuromuscular blockade during the three stages of OLT. 相似文献
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Chuan Zuo Xi-sheng Xie Hong-yu Qiu Yao Deng Da Zhu Jun-ming Fan 《Journal of Zhejiang University. Science. B》2009,10(5):380-390
Astragalus mongholicus (AM) derived from the dry root of Astragalus membranaceus Bge. var. mongolicus (Bge.) Hsiao is a widely used traditional Chinese medicine. The present study investigated the potential
role of AM on renal fibrosis on a rat model of unilateral ureteral obstruction (UUO). We divided 48 Sprague-Dawley rats randomly
into 4 groups: sham-operated group (Sham), untreated UUO group, AM-treated (10 g/(kg·d)) UUO group, and losartan-treated (20
mg/(kg·d)) UUO group as positive control. Haematoxylin & eosin (HE) and Masson staining were used to study the dynamic histological
changes of the kidneys 7 and 14 d after operation. The expressions of fibronectin (FN), type I collagen (colI), hepatocyte
growth factor (HGF), transforming growth factor-β1 (TGF-β1), and α-smooth muscle actin (α-SMA) were analyzed by real-time
polymerase chain reaction (PCR), immunohistochemistry staining, and Western blot. Results show that, similar to losartan,
AM alleviated the renal damage and decreased the deposition of FN and colI from UUO by reducing the expressions of TGF-β1
and α-SMA (P<0.05), whereas HGF increased greatly with AM treatment (P<0.05). Our findings reveal that AM could retard the progression of renal fibrosis. The renoprotective effect of AM might
be related to inhibition of myofibroblast activation, inducing of HGF and reducing of TGF-β1 expression.
Project (No. 30170437) supported by the National Natural Science Foundation of China 相似文献