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1.
甘蔗糖蜜酒精发酵污染问题   总被引:1,自引:0,他引:1  
在甘蔗糖蜜酒精生产中,由于杂菌污染而导致寄生发酵的现象普遍存在,严重时导致生产瘫痪。本文在对污染及其控制问题进行分析的基础上,指出控制污染的关键在于“抑菌”。  相似文献   

2.
温度对浓香型白酒发酵生酸的影响   总被引:1,自引:0,他引:1  
通过对控温发酵与传统发酵方法进行对照试验, 检测不同发酵期糟醅中酵母菌、兼性厌氧细菌的数量和乳酸、已酸的生成量. 发现在发酵初期控制 较低温度, 可以使酵母菌成为优势菌群, 抑制乳酸生成. 在发酵中后期控制较低温度, 可以使兼性厌氧细菌的数量出现两个峰值, 促进已酸的生成. 初步 解析了发酵温度对酵母菌、兼性厌氧细菌的影响, 以及酵母菌、兼性厌氧细菌的消长与乳酸、已酸的生成量的关系. 表明用换热方法实现对浓香型白酒发 酵温度进行控制可以降低乳酸生成量, 提高已酸生成量.  相似文献   

3.
酒精粗馏废液返回用作糖蜜稀释水,重新参与二次发酵是酒精生产废水治理减排的有效途径之一。但该项技术虽有国外的成功案件和国内的实验基础,但理论依据不足,人们心存疑虑,难以在实际生产中应用推广。为了消除人们的思想顾虑,早日实现该技术的应用推广。对酒精发酵系统的生化反应进行了分析,并利用平衡移动的观点,从理论上说明酒精粗馏废液回用后,不会对正常的酒精发酵产生不良影响。以期为该项技术的使用提供理论支持。  相似文献   

4.
以高粱为主料,香菇、豆豉为辅料生产食用醋的酒精发酵过程中,通过测定发酵产物酒精的生成量筛选适宜的发酵条件.实验结果表明:温度、接种量和发酵时间等环境因子对酒精发酵产物酒精产量影响明显.香菇保健醋酒精发酵的适宜条件为接种量10%,发酵培养温度28℃,发酵时间120 h.  相似文献   

5.
目的:研究不同有机碳源对灵芝液体发酵灵芝酸生产的影响。方法:研究不同浓度的玉米粉、小麦粉、荞麦粉下灵芝液体发酵菌丝体干重、灵芝酸含量及产量。结果:培养基玉米粉浓度4g/L时,菌丝体干重和灵芝酸产量分别达到最大值,较对照组提高了1.11和1.26倍;培养基小麦粉浓度10g/L时,干重和产量分别比对照组提高了0.73和0.87倍;培养基荞麦粉浓度7g/L时,干重和产量分别比对照组提高了0.97和1.15倍。培养基玉米粉浓度7g/L时,灵芝酸含量达到最大值23.538mg/g。培养基中玉米粉浓度为4、5、7、10g/L时,灵芝酸产量差异不显著。结论:从生产实际考虑,应使用4g/L的玉米粉优化培养基。  相似文献   

6.
通过对L脯氨酸生产菌株—嗜醋酸棒杆菌(出发菌株为谷氨酸生产菌ATCC13870)产酸机制及生理生化特点分析,掌握了该菌株高产L脯氨酸的相关条件,成功地在20m3发酵罐中将单批发酵产酸率、糖酸转化率从43%和170%分别提高到66%和250%,发酵周期缩短20%  相似文献   

7.
进行了固定化酵母与游离酵母产酒精发酵实验.通过测其酒精体积分数、酸度、糖度进行对照比较,分析固定化酵母与游离酵母在产酒精等方面的差异.结果表明,在酒精体积分数方面,固定化酵母发酵比游离酵母发酵酒精体积分数高40%左右;在酸度方面.固定化酵母发酵液比游离酵母发酵液酸度更稳定,变化更小;在糖度方面,固定化酵母发酵液比游离酵母发酵液糖度低15%左右.固定化酵母细胞在发酵产酒精方面与游离细胞相比,具有很大的优势.  相似文献   

8.
苹果营养醋的生产工艺研究   总被引:1,自引:0,他引:1  
目的:研究苹果汁的最适发酵工艺条件及醋酸发酵过程中总酸和总酯的变化。方法:分别选择糖度为8°Bx、10°Bx、12°Bx、15°Bx的苹果汁进行酒精发酵,对发酵产品的气味和酒精含量进行测定,并以影响醋酸发酵过程的重要因素接种量、发酵温度、发酵时间进行三因素三水平正交试验,测定了醋酸发酵过程中的总酸及总酯的变化。结果:采用糖度为12°Bx的苹果汁进行酒精发酵,酒精含量高,风味好,成本低;醋酸发酵的醋酸菌接种量为10%,发酵温度为32~34℃,发酵时间为72h,发酵后采用1~2个月陈酿,所得产品的有机酸含量高,风味好。结论:苹果汁酒精发酵和醋酸发酵采用适宜的糖度和发酵条件与陈酿工艺,产品的总酸含量较高,风味较好。  相似文献   

9.
青霉素发酵属单一纯菌种发酵,其发酵工艺过程为:青霉素生产菌制备→种子罐种子液制备→发酵培养→放罐.青霉素生产菌制备是青霉素发酵过程的关键环节,生产菌种的质量是影响发酵生产水平的重要因素.在生产过程中既要有优良的菌种,又要有良好的培养条件才能获得高质量的种子.  相似文献   

10.
为了实现普通生酮基古龙酸菌的单独培养和产酸,采用伴生菌巨大芽孢杆菌生长时分泌的胞外液、胞内液混合的培养方法,通过测定普通生酮基古龙酸菌单独培养时活菌数及摇瓶发酵中2-酮基-L-古龙酸(2-KGA)生成情况,研究添加伴生菌胞内液和胞外液对普通生酮基古龙酸菌生长和产酸的影响。结果表明:普通生酮基古龙酸菌利用一定量的伴生菌胞外液和胞内液可以实现普通生酮基古龙酸菌的单独生长和正常产酸。  相似文献   

11.
为了更好的推进《抗生素发酵工艺》课程改革,提高人才培养质量,结合我院课程改革经验,以工作任务为导向,从课程内容、教学方法和手段以及师资队伍建设三个方面入手,强化《抗生素发酵工艺》课程改革,体现高等职业教育的特色。  相似文献   

12.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to  相似文献   

13.
从商丘市不同环境的土壤中分离筛选到几株产脂肪酶的霉菌菌株,作者对其中的一株根霉(Rhizopus delemar)进行了液体发酵的研究,使用发酵产酶培养基组成(%):豆粉6.0,蛋白胨4.0,蔗糖1.0,K2HSO4 0.2,MgSO4·7H2O0.1,pH6.5.产酶最适温度28 ℃,接种量为1 ml,培养周期48 h,转速200 r/min.  相似文献   

14.
INTRODUCTION Saccharomycete, closely linked with human life, is the only microbe that has been utilized to the extent of over a million tons in the world, and is one of the modelled biological bacteria mostly used in modern genetic engineering. Since there are no effective online instruments for measuring the production or resultant concentration, which is a very important quality index in fermentation process, soft sensors (Martin, 1997; Tham et al., 1991; Assis and Filho, 2000; Salgad…  相似文献   

15.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9-25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9-25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation withA. mediterranei XC 9-25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation.By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentaion in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up ofrifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to a 60 m3 fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m3 fermentor and in the 60 m3 fermentor reached 17.25 g/L and 19.11 g/L, respectively.  相似文献   

16.
Study of the effect of dissolved oxygen and shear stress on rifamycin B fermentation with A. mediterranei XC 9–25 showed that rifamycin B fermentation with Amycolatoposis mediterranei XC 9–25 needs high dissolved oxygen and is not very sensitive to shearing stress. The scale-up of rifamycin B fermentation with A. mediterranei XC 9–25 from a shaking flask to a 15 L fermentor was realized by controlling the dissolved oxygen to above 25% of saturation in the fermentation process, and the potency of rifamycin B fermentation in the 15 L fermentor reached 10 g/L after 6-day batch fermentation. By continuously feeding glucose and ammonia in the fermentation process, the potency of rifamycin B fermentation in the 15 L fermentor reached 18.67 g/L, which was 86.65% higher than that of batch fermentation. Based on the scale-up principle of constantly aerated agitation power per unit volume, the scale-up of rifamycin B fed-batch fermentation with continuous feed from a 15 L fermentor to a 7 m3 fermentor and further to a 60 m3 fermentor was realized successfully. The potency of rifamycin B fermentation in the 7 m3 fermentor and in the 60 m3 fermentor reached 17.25 g/L and 19.11 g/L, respectively.  相似文献   

17.
本文通过对大枣不同处理条件的单因素试验,确定出制备枣汁的最佳工艺;用正交试验设计出合理的发酵工艺条件;最终大枣酒经发酵、沉淀后富含营养成分、具有美容养颜,促进新陈代谢等功效。  相似文献   

18.
发酵制药废水具有成分高度复杂、色度过深等特性,并且发酵制药废水属于高浓度的有机废水,只有通过处理使其达到排放标准才能避免对环境造成影响,利用三维电解对发酵制药废水进行处理可以确保其满足《发酵类制药工业污染物排放标准》(GB21903-2008)的污水排放标准要求。  相似文献   

19.
研究发酵基质对混菌固体发酵产酶的影响.采用正交实验法优化发酵培养基配方.结果表明:产CMC酶的最优培养基配方是:麸皮60 g、豆粕3 g、玉米粉7 g、稻草粉3 g和硝酸铵1 g、营养盐10%.  相似文献   

20.
The presence of yeast cells could stimulate hydrogen utilization of acetogens and enhance acetogenesis. To understand the roles of acetogens in rumen fermentation, an in vitro rumen fermentation experiment was conducted with addition of acetogen strain (TWA4) and/or Saccharomyces cerevisiae fermentation product (XP). A 2×2 factorial design with two levels of TWA4 (0 or 2×107 cells/ml) and XP (0 or 2 g/L) was performed. Volatile fatty acids (VFAs) were increased (P<0.05) in XP and TWA4XP, while methane was increased only in TWA4XP (P<0.05). The increase rate of microorganisms with formyltetrahydrofolate synthetase, especially acetogens, was higher than that of methanogens under all treatments. Lachnospiraceae was predominant in all acetogen communities, but without close acetyl-CoA synthase (ACS) amino acid sequences from cultured isolates. Low-Acetitomaculum ruminis-like ACS was predominant in all acetogen communities, while four unique phylotypes in XP treatment were all amino acid identified low-Eubacterium limosum-like acetogens. It differs to XP treatment that more low-A. ruminis-like and less low-E. limosum-like sequences were identified in TWA4 and TWA4XP treatments. Enhancing acetogenesis by supplementation with an acetogen strain and/or yeast cells may be an approach to mitigate methane, by targeting proper acetogens such as uncultured low-E. limosum-like acetogens.  相似文献   

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