共查询到20条相似文献，搜索用时 46 毫秒
1.
Li-na Zhang Yun-mei Yang Zhe-rong Xu Qi-feng Gui Qin-qing Hu 《Journal of Zhejiang University. Science. B》2010,11(9):681-689
Objective
To assess whether people who ever use any form of chewing substance in Asia are at increased risk of cardiovascular disease (CVD). 相似文献2.
3.
Shi Z Bai R Fu ZX Zhu YL Wang RF Zheng S 《Journal of Zhejiang University. Science. B》2012,13(1):11-19
Objective
We aimed to perform a preliminary study of the association between induced pluripotent stem cell (iPS)-related genes and biological behavior of human colorectal cancer (CRC) cells, and the potential for developing anti-cancer drugs targeting these genes. 相似文献4.
Objective
To investigate gene mutations associated with three different types of corneal dystrophies (CDs), and to establish a phenotype-genotype correlation. 相似文献5.
Ning ZHANG Jing JIANG Yan-li YANG Zhi-he WANG 《Journal of Zhejiang University. Science. B》2015,16(10):845-856
In this study, we produced tomato plants overexpressing an invertase inhibitor gene (Sly-INH) from tomato, using a simple and efficient transient transformation system. Compared with control plants, the expression of Sly-INH was highly upregulated in Sly-INH overexpressing plants, as indicated by real-time polymerase chain reaction (PCR). Physiological analysis revealed that Sly-INH inhibited the activity of cell wall invertase (CWIN), which increased sugar accumulation in tomato fruit. Furthermore, Sly-INH mediated sucrose metabolism by regulating CWIN activity. Our results suggest that invertase activity is potentially regulated by the Sly-INH inhibitor at the post-translational level, and they demonstrate that the transient transformation system is an effective method for determining the functions of genes in tomato. 相似文献
6.
Zhu R Chen YP Deng YY Zheng R Zhong YF Wang L Du LP 《Journal of Zhejiang University. Science. B》2011,12(12):1024-1033
Background and Objectives
Chronic kidney disease (CKD) is a growing public health problem with an urgent need for new pharmacological agents. Cordyceps cicadae is widely used in traditional Chinese medicine (TCM) and has potential renoprotective benefits. The current study aimed to determine any scientific evidence to support its clinical use. 相似文献7.
Yan-ju Li Neng Zhu Hai-quan Jia Jin-hui Wu Ying Yi Jian-cheng Qi 《Journal of Zhejiang University. Science. B》2012,13(4):254-260
Objective
Chlorine dioxide (CD) gas has been used as a fumigant in the disinfection of biosafety laboratories. In this study, some experiments were conducted to assess the inactivation of spores inoculated on six materials [stainless steel (SS), painted steel (PS), polyvinyl chlorid (PVC), polyurethane (PU), glass (GS), and cotton cloth (CC)] by CD gas. The main aims of the study were to determine the sporicidal efficacy of CD gas and the effect of prehumidification before decontamination on sporicidal efficacy. 相似文献8.
Objective
The gene for mast cell chymase (CMA1) is an ideal candidate for investigating the genetic predisposition to coronary heart disease (CHD), as activated mast cells have been found to be present in a greater proportion in the shoulder region of atheroma than in normal coronary intimae. Previous studies have indicated that CMA1 promoter polymorphism rs1800875 may be involved in regulating immunoglobulin E (IgE) levels in patients with eczema, and it is associated with the progression of immunoglobulin A nephropathy. 相似文献9.
Wang LR Zhang GB Chen J Li J Li MW Xu N Wang Y Shen Tu JZ 《Journal of Zhejiang University. Science. B》2011,12(3):174-179
Objective
To evaluate the predictive values of gene expressions of ribonucleotide reductase M1 (RRM1) and breast cancer susceptibility gene 1 (BRCA1) in peripheral blood from Chinese patients with non-small-cell lung cancer (NSCLC) treated with gemcitabine plus platinum. 相似文献10.
11.
Eva-Guadalupe LIZRRAGA-PAULíN Susana-Patricia MIRANDA-CASTRO Ernesto MORENO-MARTíNEZ Alma-Virginia LARA-SAGAHóN Irineo TORRES-PACHECO 《Journal of Zhejiang University. Science. B》2013,14(2):87-96
Objective:To evaluate the effect of chitosan(CH) and hydrogen peroxide(H2O2) seed coatings and seedling sprinklings on two different maize varieties by measuring their phenology,the H2O2 presence,the catalase(CAT) activity,and the protein quantity.Methods:Seven groups of ten seeds for each maize variety were treated with CH(2%(20 g/L) and 0.2%(2 g/L)) or H2O2(8 mmol/L) by coating,sprinkling,or both.Germination and seedling growth were measured.One month after germination,the presence of H2O2 in seedlings in the coated seed treatments was evaluated.Protein content and CAT activity were determined under all treatments.Results:H2O2 seed coating enhanced the germination rate and increased seedling and stem length in the quality protein maize(QPM) variety.Seedlings had a higher emergence velocity under this treatment in both varieties.CH and H2O2 sprinklings did not have an effect on seedling phenology.Exogenous application of H2O2 promoted an increase of endogenous H2O2.CH and H2O2 seedling sprinkling increased the protein content in both maize varieties,while there was no significant effect on the CAT activity of treated seeds and seedlings.Conclusions:CH and H2O2 enhance some phenological and biochemical features of maize depending on their method of application. 相似文献
12.
Objective
In order to overcome the defects of chemical hydrolysis approach to prepare glucosamine, an enzymatic hydrolysis method was developed. 相似文献13.
14.
Hai-qin Zhong Shun Zhang Hong Pan Ting Cai 《Journal of Zhejiang University. Science. B》2013,14(9):837-843
Objective
The efflux pump (EP) is one of the major mechanisms of antibiotic resistance in Klebsiella pneumoniae. However, there are few reports on the effect of the abuse of antibiotic use on the activity of EPs. To determine whether the use of low efficacy antibiotics has any effect on the activity of EPs and induces drug resistance in K. pneumoniae, we investigated the effect of ciprofloxacin on the activity of EPs in K. pneumoniae strains.Methods
Sixteen susceptible K. pneumoniae strains were isolated from patients and their minimum inhibitory concentrations (MICs) of ciprofloxacin were measured in the absence and presence of the pump inhibitor carbonyl cyanide m-chlorophenyl hydrazone (CCCP). The strains were then induced with a gradient of ciprofloxacin until the MICs of the strains showed no further increase, to obtain induced resistant strains. The EP activities of the strains before and after induction were compared using EP inhibition and ethidium bromide (EtBr) accumulation assays.Results
The MIC values of the strains were 16–256 times higher after induction than before induction. In the presence of CCCP, the MIC values of 50% of the induced strains were 2–4-fold lower than that in the absence of this inhibitor. The EtBr accumulation assay showed that the fluorescence of EtBr in the induced cells was lower than that in the cells before induction.Conclusions
EPs are widespread in susceptible and drug-resistant K. pneumoniae strains. Induction with ciprofloxacin may increase the activity of EPs in K. pneumoniae. The EtBr accumulation assay is more sensitive than the EP inhibition assay in evaluating the activity of EPs in K. pneumoniae. 相似文献15.
Shuang LIANG Xue-lai WANG Ming-yang ZOU Han WANG Xue ZHOU Cai-hong SUN Wei XIA Li-jie WU Takashi X. FUJISAWA Akemi TOMODA 《Journal of Zhejiang University. Science. B》2014,15(3):264-271
Objective
A study in a Caucasian population has identified two single-nucleotide polymorphisms (SNPs) in ZNF533, one in DOCK4, and two in IMMP2L, which were all significantly associated with autism. They are located in AUTS1 and AUTS5, which have been identified as autism susceptibility loci in several genome-wide screens. The present study aimed to investigate whether ZNF533, DOCK4, and IMMP2L genes are also associated with autism in a northeastern Chinese Han population.Methods
We performed a similar association study using families with three individuals (one autistic child and two unaffected parents). A family-based transmission disequilibrium test (TDT) was used to analyze the results.Results
There were significant associations between autism and the two SNPs of ZNF533 gene (rs11885327: χ 2=4.5200, P=0.0335; rs1964081: χ 2=4.2610, P=0.0390) and the SNP of DOCK4 gene (rs2217262: χ 2=5.3430, P=0.0208).Conclusions
Our data suggest that ZNF533 and DOCK4 genes are linked to a predisposition to autism in the northeastern Chinese Han population. 相似文献16.
Anna Pick Kiong LING Kinn Poay TAN Sobri HUSSEIN 《Journal of Zhejiang University. Science. B》2013,14(7):621-631
Objective: Labisia pumila var. alata, commonly known as ’Kacip Fatimah’ or ’Selusuh Fatimah’ in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs. 相似文献
17.
Ning Zhou Wei-xing Chen Wei Zhang Lan Li Xi Jin You-ming Li 《Journal of Zhejiang University. Science. B》2010,11(9):690-701
Objective:The aim of our study was to perform a systematic review and meta-analysis of the efficacy of short-term protocols for Helicobacter pylori eradication and to review the safety and adverse profiles of these eradication protocols.Methods:Literatures were located through electronic searches by PubMed,Medline,ISI Web of Knowledge,and Cochrane Library using the relevant terms.Abstracts of important meetings were searched manually in some journal supplements.Additional bibliographies were identified from the reference lists of identified studies.Three independent reviewers systemically identified randomized controlled trials(RCTs) comparing short-duration protocols vs.7-d proton pump inhibitor(PPI)-based triple protocols,as well as studies reporting eradication rates of short-duration protocols for H.pylori.Summary effect size was calculated as relative risk(RR) and 95% confidence intervals(CI) using Review Manager 4.2,and P0.05 was defined as statistically significant in all analyses.Results:Among 90 abstracts retrieved,15 studies were analyzed,including a total of 30 treatment regimens with 1856 subjects.Mean intention-to-treat(ITT) cure rates of 63.2% and 81.3% were achieved with short-term protocols and 7-d PPI-containing protocols,respectively.Per-protocol(PP)-based overall cure rates were 66.6% and 86.1%,respectively.Short-term therapy was inferior to 7-d triple regimen(P0.00001).After sub-analysis,however,comparing the effects of ≥3-d protocols and 7-d triple protocols,the cumulative ITT RR was 0.95(P=0.26),and PP RR was 0.95(P=0.10),without significant heterogeneity.Moreover,slightly fewer adverse-effects were found in short-term protocols.Conclusions:Although more economical,short-duration protocols are inferior to 7-d PPI-based triple protocols with regarding to eradication rate of H.pylori.Protocols of more than 3 d,however,may be equivalent to 7-d protocols. 相似文献
18.
Guo-dong HAO Yi-wu ZHENG Birgitte GJESING Xing-ai KONG Jing-yuan WANG Zhi-jing SONG Xu-xin LAI Nan-shan ZHONG Michael D.SPANGFORT 《Journal of Zhejiang University. Science. B》2013,14(3):240-246
Objective:Weed pollens are common sources of allergens worldwide.The prevalence of weed pollen sensitization is not yet fully known in China.The purpose of this study was to investigate the prevalence of sensitization to weed allergens from Artemisia,Ambrosia,and Humulus in northern China.Methods:A total of 1144 subjects(aged from 5 to 68 years) visiting our clinic from June to October 2011 underwent intradermal testing using a panel of 25 allergen sources.Subjects with positive skin responses to any pollen were further tested for their serum concentrations of IgE antibodies against Artemisia vulgaris,Ambrosia artemisiifolia,and Humulus scandens,and against the purified allergens,Art v 1 and Amb a 1.Results:Of 1144 subjects,170 had positive intradermal reactions to pollen and 144 donated serum for IgE testing.The prevalence of positive intradermal responses to pollens of Artemisia sieversiana,Artemisia annua,A.artemisiifolia,and H.scandens was 11.0%,10.2%,3.7%,and 6.6%,respectively.Among the intradermal positive subjects,the prevalence of specific IgE antigens to A.vulgaris was 58.3%,to A.artemisiifolia 14.7%,and to H.scandens 41.0%.The prevalence of specific IgE antigens to the allergen Art v 1 was 46.9%,and to Amb a 1 was 11.2%.The correlation between the presence of IgE antibodies specific to A.vulgaris and to the Art v 1 antigen was very high.Subjects with A.artemisiifolia specific IgE also had A.vulgaris specific IgE,but with relatively high levels of A.vulgaris IgE antibodies.There were no correlations between the presence of IgE antibodies to H.scandens and A.vulgaris or to H.scandens and A.artemisiifolia.Conclusions:The intradermal prevalence of weed pollen sensitization among allergic subjects in northern China is about 13.5%.Correlations of specific IgE antibodies suggest that pollen allergens from Artemisia and Humulus are independent sources for primary sensitization. 相似文献
19.
Feng-wei Song Bin-bin Chen Zhao-hui Sun Li-ping Wu Su-juan Zhao Qi Miao Xia-jing Tang 《Journal of Zhejiang University. Science. B》2013,14(6):479-486
Objective
To screen mutations in FERM domain-containing protein 7 (FRMD7) gene in two Chinese families with X-linked idiopathic congenital nystagmus (XLICN).Methods
Common ophthalmic data and peripheral blood of two Chinese XLICN families (families A and B) were collected after informed consent. Genomic DNA was prepared from the peripheral blood of members of the two families and from 100 normal controls. Mutations in the FRMD7 gene were determined by directly sequencing polymerase chain reaction (PCR) products.Results
We identified a novel mutation c.980_983delATTA compound with c.986C>A mutation in the 11th exon of FRMD7 in family B, and a previously reported splicing mutation c.782G>C (p.R261G) in family A. The mutations were detected in patients and female carriers, while they were absent in other relatives or in the 100 normal controls.Conclusions
Our results expand the spectrum of FRMD7 mutations in association with XLICN, and further confirm that the mutations of FRMD7 are the underlying molecular mechanism for XLICN. 相似文献20.
Shiau Mei WOON Yew Wei SENG Anna Pick Kiong LING Soi Moi CHYE Rhun Yian KOH 《Journal of Zhejiang University. Science. B》2014,15(3):295-302