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1.
BackgroundBiomineralization is a significant process performed by living organisms in which minerals are produced through the hardening of biological tissues. Herein, we focus on calcium carbonate precipitation, as part of biomineralization, to be used in applications for environmental protection, material technology, and other fields. A strain GM-1, Microbacterium sp. GM-1, isolated from active sludge, was investigated for its ability to produce urease and induce calcium carbonate precipitation in a metabolic process.ResultsIt was discovered that Microbacterium sp. GM-1 resisted high concentrations of urea up to 60 g/L. In order to optimize the calcification process of Microbacterium sp. GM-1, the concentrations of Ni2 + and urea, pH value, and culture time were analyzed through orthogonal tests. The favored calcite precipitation culture conditions were as follows: the concentration of Ni2 + and urea were 50 μM and 60 g/L, respectively, pH of 10, and culture time of 96 h. Using X-ray diffraction analysis, the calcium carbonate polymorphs produced by Microbacterium sp. GM-1 were proven to be mainly calcite.ConclusionsThe results of this research provide evidence that Microbacterium sp. GM-1 can biologically induce calcification and suggest that strain GM-1 may play a potential role in the synthesis of new biominerals and in bioremediation or biorecovery.  相似文献   

2.
The essential content of a recent paper by the present writer comprises a comprehensive discussion of the physical bases underlying derivation of formulas for calculating the temperature distribution T, maximum temperature Tm and average temperature Ta in a toroidal electrical coil of rectangular cross section, internally generated heat and change of wire resistance with temperature being taken into account. Illustratively, the solution for the boundary value condition of constant surface temperature and uniform equivalent thermal conductivity was obtained.For the most part, however, problems that arise in practice are not encompassed in the comparatively simple boundary conditions of constant temperature. Experiment shows that in general the boundary condition is T ? T′ = ? K??n; whereof n denotes the outward drawn normal to the coil surface, K = (knh) the ratio of the equivalent thermal conductivity in the direction of n to the emissivity of the boundary surface, and T and T′ are the corresponding temperatures in the coil surface and the immediately adjacent ambient medium. Again, it frequently ensues in practice that the thermal conductivity is substantially different in the directions of the two principal axes of the cross section.In the present paper formulas for T, Tm, and Ta are obtained for electrical coils of ratio of external to internal radius greater than (roughly) two whereof (i) the thermal conductivity is different in the directions of the two principal axes of the cross section, (ii) K is different on but constant over each of the four faces of the coil, and (iii) no restriction is made as to T′ except that over each face it be expressible in a generalized Fourier series. Determination of T is posed as a boundary-value problem in the mathematical theory of heat; the formal solution of T effected by expansions in orthogonal functions; and Tm and Ta then determined through use of their known relationships with T. The resulting formulas are in the form of rapidly-converging singly-infinite trigonometric-hyperbolic series. Illustrative of application of these general formulas, the maximum temperatures in a coil of given dimensions subject to two different sets of surface conditions are calculated and found to be in excellent agreement with the known measured values.The just-mentioned formulas encompass practically all cases encountered in practice except those coils which do not satisfy the restriction as to ratio of radii. For these latter formulas for T, Tm, and Ta are obtained pursuant to conditions of (i) equivalent thermal conductivity different in the directions of the two principal axes of the cross section, (ii) K, and likewise T′, different on but constant over each of the four faces of the coil. These formulas are in the form of rapidly-converging singly-infinite trigonometric- Bessel function (of zero order) series: Illustratively, the maximum temperature in a coil of given dimensions is calculated and found to be in excellent agreement with the known measured value.  相似文献   

3.
For the diagnosis of biochemical reactions, the investigation of microflow behavior, and the confirmation of simulation results in microfluidics, experimentally quantitative measurements are indispensable. To characterize the mixing and reaction of fluids in microchannel devices, we propose a mixing quality index (Mqi) to quantify the cross-sectional patterns (also called mixing patterns) of fluids, captured with a confocal-fluorescence microscope (CFM). The operating parameters of the CFM for quantification were carefully tested. We analyzed mixing patterns, flow advection, and mass exchange of fluids in the devices with overlapping channels of two kinds. The mixing length of the two devices derived from the analysis of Mqi is demonstrated to be more precise than that estimated with a commonly applied method of blending dye liquors. By means of fluorescence resonance-energy transfer (FRET), we monitored the hybridization of two complementary oligonucleotides (a FRET pair) in the devices. The captured patterns reveal that hybridization is a progressive process along the downstream channel. The FRET reaction and the hybridization period were characterized through quantification of the reaction patterns. This analytical approach is a promising diagnostic tool that is applicable to the real-time analysis of biochemical and chemical reactions such as polymerase chain reaction (PCR), catalytic, or synthetic processes in microfluidic devices.  相似文献   

4.
The supramolecular chemistry of nanoclusters is a flourishing area of nano-research; however, the controllable assembly of cluster nano-building blocks in different arrays remains challenging. In this work, we report the hierarchical structural complexity of atomically precise nanoclusters in micrometric linear chains (1D array), grid networks (2D array) and superstructures (3D array). In the crystal lattice, the Ag29(SSR)12(PPh3)4 nanoclusters can be viewed as unassembled cluster dots (Ag29–0D). In the presence of Cs+ cations, the Ag29(SSR)12 nano-building blocks are selectively assembled into distinct arrays with different oxygen-carrying solvent molecules―Cs@Ag29(SSR)12(DMF)x as 1D linear chains (Ag29–1D), Cs@Ag29(SSR)12(NMP)x as 2D grid networks (Ag29–2D), and Cs@Ag29(SSR)12(TMS)x as 3D superstructures (Ag29–3D). Such self-assemblies of these Ag29(SSR)12 units have not only been observed in their crystalline state, but also in their amorphous state. Due to the diverse surface structures and crystalline packing modes, these Ag29-based assemblies manifest distinguishable optical absorptions and emissions in both solutions and crystallized films. Furthermore, the surface areas of the nanocluster crystals are evaluated, the maximum value of which occurs when the cluster nano-building blocks are assembled into 2D arrays (i.e. Ag29–2D). Overall, this work presents an exciting example of the hierarchical assembly of atomically precise nanoclusters by simply controlling the adsorbed molecules on the cluster surface.  相似文献   

5.
Control problems in Hilbert spaces are treated in a measure-theoretical framework; instead of dealing with a set of admissible trajectory-control pairs, a set of measures defined by the boundary conditions and the differential equations of the problem are considered. The concept of weak controllability is introduced; a system has this property if every pair of initial and final points, (ta,xa) and (tb,xb) can be weakly joined; this is possible if a set of linear equalities involving measures has a solution. In turn, this is shown to be equivalent to the possibility of extending a linear functional in a positive manner. Necessary and sufficient conditions for controllability are derived, and applied to the study of a finite-dimensional system with the control appearing linearly.  相似文献   

6.
Huang SH  Hsueh HJ  Jiang YL 《Biomicrofluidics》2011,5(3):34109-3410910
This paper describes a light-addressable electrolytic system used to perform an electrodeposition of calcium alginate hydrogels using a digital micromirror device (DMD). In this system, a patterned light illumination is projected onto a photoconductive substrate serving as a photo-anode to electrolytically produce protons, which can lead to a decreased pH gradient. The low pH generated at the anode can locally release calcium ions from insoluble calcium carbonate (CaCO3) to cause gelation of calcium alginate through sol-gel transition. By controlling the illumination pattern on the DMD, a light-addressable electrodeposition of calcium alginate hydrogels with different shapes and sizes, as well as multiplexed micropatterning was performed. The effects of the concentration of the alginate and CaCO3 solutions on the dimensional resolution of alginate hydrogel formation were experimentally examined. A 3 × 3 array of cell-encapsulated alginate hydrogels was also successfully demonstrated through light-addressable electrodeposition. Our proposed method provides a programmable method for the spatiotemporally controllable assembly of cell populations into cellular microarrays and could have a wide range of biological applications in cell-based biosensing, toxicology, and drug discovery.  相似文献   

7.
Gold nanoparticles (Au NPs) were directly synthesized on the surface of polyvinylsilazane (PVSZ, -[(vinyl)SiH-NH2]-) without use of extra reductive additives. The reductive Si-H functional groups on the surface of cured PVSZ acted as surface bound reducing agents to form gold metal when contacted with an aqueous Au precursor (HAuCl4) solution, leading to formation of Au NPs adhered to silicate glass surface. The Au NPs-silicate platforms were preliminarily tested to detect Rhodamine B (1 μM) by surface enhanced Raman scattering. Furthermore, gold microelectrode obtained by post-chemical plating was used as an integrated amperometric detection element in the polydimethylsilane-glass hybrid microfluidic chip.  相似文献   

8.
镉(Cd)是一种剧毒重金属元素,可引起呼吸系统、肾损伤等多种疾病,对人体毒性极高,可通过多种途径暴露于人体,危害人体健康。土壤-植物-食品-人的迁移途径在人类对环境镉的暴露中占主导地位。近年来,Cd通过饮食途径对人体健康造成的风险日益引起人们的关注和重视。本文在对研究区不同土地利用类型下土壤和作物进行大规模采样调查的基础上,对其蔬菜、水稻、旱地、果园等4种土地类型土壤及其种植作物中镉含量积累进行了研究。结果表明,菜地和水稻地有明显的污染超标现象,其中菜地土壤镉含量最高。根据不同土地利用类型下各作物镉富集系数,进行聚类分析,比较其对镉富集能力的差异,结果表明水稻地稻米镉的富集能力最高,单独划为一类。利用定性风险分析技术,对不同土地利用类型下土壤镉对人体健康的风险商值进行了计算,结果表明不同土地类型下,作物镉风险商值为水稻>深色蔬菜>浅色蔬菜>水果>玉米。镉对个人致癌年风险的计算结果表明,水稻个人年致癌风险为0.6270×10-4/年,高于防护委员会(ICRP)推荐的最大可接受年风险水平0.5×10-4/年,为不可接受水平,居民面临较大的致癌风险,应采取积极措施对研究区土壤镉污染进行治理和修复。  相似文献   

9.
Anomalies occurring in electron diffraction patterns of etched An leaf films were studied. The anomalies were of two kinds, extra lines and orientation effects. The extra lines, in agreement with the report of Thomson and Cochrane, were attributed to contamination of the films by some long chain compound. The orientation effects consisted of a variation of the intensities of several of the lines of the An patterns obtained from the intensity of these same lines as observed in standard patterns of randomly-oriented Au. These abnormal intensities evidenced the existence of the [100] crystal direction as an axis of orientation perpendicular to the face of the film. A second orientation effect, the arcing of some of the lines of the Au patterns, indicated a second axis of orientation parallel to the face of the film but insufficient evidence prevented the identification of this axis of orientation.Results of the orientation study confirm the findings of other investigators, particularly those of Trillat and Hirsch.  相似文献   

10.
Mechanically exfoliated two-dimensional ferromagnetic materials (2D FMs) possess long-range ferromagnetic order and topologically nontrivial skyrmions in few layers. However, because of the dimensionality effect, such few-layer systems usually exhibit much lower Curie temperature (TC) compared to their bulk counterparts. It is therefore of great interest to explore effective approaches to enhance their TC, particularly in wafer-scale for practical applications. Here, we report an interfacial proximity-induced high-TC 2D FM Fe3GeTe2 (FGT) via A-type antiferromagnetic material CrSb (CS) which strongly couples to FGT. A superlattice structure of (FGT/CS)n, where n stands for the period of FGT/CS heterostructure, has been successfully produced with sharp interfaces by molecular-beam epitaxy on 2-inch wafers. By performing elemental specific X-ray magnetic circular dichroism (XMCD) measurements, we have unequivocally discovered that TC of 4-layer Fe3GeTe2 can be significantly enhanced from 140 K to 230 K because of the interfacial ferromagnetic coupling. Meanwhile, an inverse proximity effect occurs in the FGT/CS interface, driving the interfacial antiferromagnetic CrSb into a ferrimagnetic state as evidenced by double-switching behavior in hysteresis loops and the XMCD spectra. Density functional theory calculations show that the Fe-Te/Cr-Sb interface is strongly FM coupled and doping of the spin-polarized electrons by the interfacial Cr layer gives rise to the TC enhancement of the Fe3GeTe2 films, in accordance with our XMCD measurements. Strikingly, by introducing rich Fe in a 4-layer FGT/CS superlattice, TC can be further enhanced to near room temperature. Our results provide a feasible approach for enhancing the magnetic order of few-layer 2D FMs in wafer-scale and render opportunities for realizing realistic ultra-thin spintronic devices.  相似文献   

11.
The rate of evaporation of electrons, νe, from a thoriated tungsten filament depends on the temperature T and on θ, the fraction of the surface covered by thorium atoms. The relation of νe to θ and T has been given by Brattain and Becker. From νe the change in contact potential V of the filament surface produced by the adsorbed thorium can be calculated by the Boltzmann equation. Knowing σ the number of thorium atoms per cm.3, the dipole moment M of each is given by V = 2τσM. By an equation already used for Cs films on tungsten, the 2-dimensional equation of state of the adsorbed film can be calculated from M. Then by Gibbs' adsorption equation the relation of the atom evaporation rate νa to θ and T can be determined. It is thus calculated that in the range from θ = 0.2 to θ = 0.6, νa varies in proportion to ?Hθ where H = 8.1 in good agreement with the value H = 7.8 given by Brattain and Becker's measurements. A recalculation of the data of numerous experiments in 1921–1923, using the new relations of νe to θ1 gives data on νa as a function of T and θ in good agreement with the values of νa calculated from νe.The diffusion coefficients of Th through tungsten crystals, along grain boundaries and over the free filament surface, are calculated. A theory is given for the cause of the variation in the surface diffusion coefficient with σ. The probable mechanism of the production of the metallic thorium within the filament is discussed. At 2400° the thorium which arrives at the surface along grain boundaries, for some unknown reason, does not spread out over the surface as it does at lower temperatures (1900–2100°).  相似文献   

12.
A numerical modeling of natural convection under the influence of either axial (Bz) or radial (Br) magnetic field in a cylindrical configuration filled with a low-Prandtl number electrically conducting fluid, is studied. The finite volume method is used to discretize the equations of continuity, Navier Stokes and energy. A computer program based on the SIMPLER algorithm is developed. The flow and temperature fields are presented by stream function and isotherms, respectively. Stability diagrams are established according to the numerical results of this investigation. These diagrams put in evidence the dependence of the critical Grashof number, Grcr with the increase of the Hartmann number, Ha. The strongest stabilization of the convective flows occurs when the magnetic field is applied in the radial direction. This study confirms the possibility of stabilization of a liquid metal flow in natural convection by application of a radial magnetic field.  相似文献   

13.
In this paper we study boundary value problems for anisotropic partial differential-operator equations with parameters. The principal part of the appropriate differential operators are not self-adjoint. Several conditions for the uniform separability in weighted Banach-valued Lp-spaces are given. Sharp estimates for the resolvent of the corresponding differential operator are obtained. In particular the positivity and R-positivity of these operators are established. As an application we study the separability of degenerate DOEs, maximal regularity for degenerate abstract parabolic problem with parameters, the uniform separability of finite and infinite systems for degenerate anisotropic partial differential equations with parameters.  相似文献   

14.
The six-membered ring (SMR) is a common structure unit for numerous material systems. These materials include, but are not limited to, the typical two-dimensional materials such as graphene, h-BN, and transition metal dichalcogenides, as well as three-dimensional materials such as beryllium, magnesium, MgB2 and Bi2Se3. Although many of these materials have already become ‘stars’ in materials science and condensed-matter physics, little attention has been paid to the roles of the SMR unit across a wide range of compositions and structures. In this article, we systematically analyze these materials with respect to their very basic SMR structural unit, which has been found to play a deterministic role in the occurrence of many intriguing properties and phenomena, such as Dirac electronic and phononic spectra, superconductivity and topology. As a result, we have defined this group of materials as SMR inorganic materials, opening up a new perspective on materials research and development. With their unique properties, SMR materials deserve wide attention and in-depth investigation from materials design, new physical discoveries to target-wizard applications. It is expected that SMR materials will find niche applications in next-generation information technology, renewable energy, space, etc.  相似文献   

15.
Heavy metals are natural elements in the Earth’s crust that can enter human food through industrial or agricultural processing, in the form of fertilizers and pesticides. These elements are not biodegradable. Some heavy metals are known as pollutants and are toxic, and their bioaccumulation in plant and animal tissues can cause undesirable effects for humans; therefore, their amount in water and food should always be under control. The aim of this study is to investigate the conditions for the bioremediation of heavy metals in foods. Various physical, chemical, and biological methods have been used to reduce the heavy metal content in the environment. During the last decades, bioremediation methods using plants and microorganisms have created interest to researchers for their advantages such as being more specific and environmentally friendly. The main pollutant elements in foods and beverages are lead, cadmium, arsenic, and mercury, which have their own permissible limits. Among the microorganisms that are capable of bioremediation of heavy metals, Saccharomyces cerevisiae is an interesting choice for its special characteristics and being safe for humans, which make it quite common and useful in the food industry. Its mass production as the byproduct of the fermentation industry and the low cost of culture media are the other advantages. The ability of this yeast to remove an individual separated element has also been widely investigated. In countries with high heavy metal pollution in wheat, the use of S. cerevisiae is a native solution for overcoming the problem of solution.This article summarizes the main conditions for heavy metal absorption by S. cerevisiae.How to cite: Massoud R, Hadiani MR, Khosravi Darani K, et al. Bioremediation of heavy metals in food industry: Application of Saccharomyces cerevisiae. Electron J Biotechnol 2019;37. https://doi.org/10.1016/j.ejbt.2018.11.003.  相似文献   

16.
Couple-stress solutions are obtained near the hole boundary of an infinite elastic plane subjected to a radial concentrated force at x = b, y = 0,under the restriction of r/b<-0.2 and b/l?;-10. Here r and l are the distance from the center of the hole to the point of interest and a characteristic length (couple-stress constant). At r = a, θ = ±π2, the stress ratio of the couple-stress solution to the classical one for the current problem is slightly lower than one of the Mindlin problem (1) for some values of v, except for v = 0.5 where they are equal. The dimensionless stress σ&#x0302;θθ is found along the hole boundary.  相似文献   

17.
Synthetic lethality was proposed nearly a century ago by geneticists and recently applied to develop precision anti-cancer therapies. To exploit the synthetic lethality concept in the design of chemical anti-cancer agents, we developed a bio-orthogonally catalyzed lethality (BCL) strategy to generate targeting anti-tumor metallodrugs both in vitro and in vivo. Metallodrug Ru-rhein was generated from two non-toxic species Ru-N3 and rhein-alkyne via exclusive endogenous copper-catalyzed azide alkyne cycloaddition (CuAAC) reaction without the need of an external copper catalyst. The non-toxic species Ru-arene complex Ru-N3 and rhein-alkyne were designed to perform this strategy, and the mitochondrial targeting product Ru-rhein was generated in high yield (>83%) and showed high anti-tumor efficacy in vitro. This BCL strategy achieved a remarkable tumor suppression effect on the tumor-bearing mice models. It is interesting that the combination of metal-arene complexes with rhein via CuAAC reaction could transform two non-toxic species into a targeting anti-cancer metallodrug both in vitro and in vivo, while the product Ru-rhein was non-toxic towards normal cells. This is the first example that exclusive endogenous copper was used to generate metal-based anti-cancer drugs for cancer treatment. The anti-cancer mechanism of Ru-rhein was studied and autophagy was induced by increased reactive oxygen species and mitochondrial damage. The generality of this BCL strategy was also studied and it could be extended to other metal complexes such as Os-arene and Ir-arene complexes. Compared with the traditional methods for cancer treatment, this work presented a new approach to generating targeting metallodrugs in vivo via the BCL strategy from non-toxic species in metal-based chemotherapy.  相似文献   

18.
In recent works several authors have considered the L1 fidelity term, the L2 fidelity term and the combined L1 and L2 fidelity term for denoising models, and they used the fast Fourier transform (FFT) algorithm which can only use periodic boundary conditions (BCs). In this paper, we combine the augmented Lagrangian method (ALM) and the symmetric Red–Black Gauss–Seidel (SRBGS) method to propose three algorithms that are suitable for different BCs. Experimental results show that the proposed algorithms are effective and the model with the combined L1 and L2 fidelity term demonstrates more advantages in efficiency and accuracy than other models with the L1 fidelity term or the L2 fidelity term.  相似文献   

19.
As the reaction product of subducted water and the iron core, FeO2 with more oxygen than hematite (Fe2O3) has been recently recognized as an important component in the D” layer just above the Earth''s core-mantle boundary. Here, we report a new oxygen-excess phase (Mg, Fe)2O3+δ (0 < δ < 1, denoted as ‘OE-phase’). It forms at pressures greater than 40 gigapascal when (Mg, Fe)-bearing hydrous materials are heated over 1500 kelvin. The OE-phase is fully recoverable to ambient conditions for ex situ investigation using transmission electron microscopy, which indicates that the OE-phase contains ferric iron (Fe3+) as in Fe2O3 but holds excess oxygen through interactions between oxygen atoms. The new OE-phase provides strong evidence that H2O has extraordinary oxidation power at high pressure. Unlike the formation of pyrite-type FeO2Hx which usually requires saturated water, the OE-phase can be formed with under-saturated water at mid-mantle conditions, and is expected to be more ubiquitous at depths greater than 1000 km in the Earth''s mantle. The emergence of oxygen-excess reservoirs out of primordial or subducted (Mg, Fe)-bearing hydrous materials may revise our view on the deep-mantle redox chemistry.  相似文献   

20.
Geophysical observations suggest that the transition zone is wet locally. Continental and oceanic sediment components together with the basaltic and peridotitic components might be transported and accumulated in the transition zone. Low-velocity anomalies at the upper mantle–transition zone boundary might be caused by the existence of dense hydrous magmas. Water can be carried farther into the lower mantle by the slabs. The anomalous Q and shear wave regions locating at the uppermost part of the lower mantle could be caused by the existence of fluid or wet magmas in this region because of the water-solubility contrast between the minerals in the transition zone and those in the lower mantle. δ-H solid solution AlO2H–MgSiO4H2 carries water into the lower mantle. Hydrogen-bond symmetrization exists in high-pressure hydrous phases and thus they are stable at the high pressures of the lower mantle. Thus, the δ-H solid solution in subducting slabs carries water farther into the bottom of the lower mantle. Pyrite FeO2Hx is formed due to a reaction between the core and hydrated slabs. This phase could be a candidate for the anomalous regions at the core–mantle boundary.  相似文献   

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