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由O’Farrel等1975年建立的双向电泳技术是目前唯一能将数千种蛋白质同时分离与展示的分离技术。双向电泳的原理是，第一向进行等电聚焦(isoelectric focusing，IEF)，蛋白质沿pH梯度分离至各自的等电点；随后，再沿垂直的方向进行SDS—PAGE电泳，按分子量大小进行分离。样品经过电荷和质量两次分 相似文献
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现代生物学表明，所有生物性状的变化是基因表达变化的结果．双向电泳是目前蛋白质常规分析技术中分辨率较高的方法．采用双向电泳技术分析了正常大鼠、心肌缺血模型大鼠、药物处理后的大鼠的血清蛋白组的差异，从而探讨利用双向电泳技术分析药物处理的有效性． 相似文献
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双向电泳技术 总被引：5，自引：0，他引：5
由O'Farrel等1975年建立的双向电泳技术是目前唯一能将数千种蛋白质同时分离与展示的分离技术。双向电泳的原理是,第一向进行等电聚焦(isoelectric focusing,IEF),蛋白质沿pH梯度分离至各自的等电点;随后,再沿垂直的方向进行SDS-PAGE电泳,按分子量大小进行分离。样品经过电荷和质量两次分离后,得到等电点和分子量的信息。电泳的结果不是蛋白质条带,而是点。在第一向等电聚焦电泳中,具有pH梯度的介质其分布是从阳极到阴极,pH逐渐增大,由于蛋白质具有两性解离和等电点的特征,在碱性区域蛋白质分子带负电荷向阳极移动,直至某一pH位点时失去电荷而停止移动,此处介质的pH恰好等于聚焦蛋白质分子的等电点。同理,位于酸性区域的蛋白质分子带正电荷向阴极移动,直到在它们的等电点 相似文献
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夏亚：今天为大家带来的是在IEF上举行的一场比赛，在这场来自MV的比赛上，mouz.TH000跟nGize．Check会给我们带来怎样的龙争虎斗呢？ 相似文献
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双向电泳技术是目前最常用的能够从植物组织中分离出上千种蛋白的技术．因此,需对蛋白样品制备过程、等电聚焦、第二相电泳、染色等进行分析探讨,结果表明,样品制备需根据样品的成分选择不同的裂解液成分（如：CHAPS,Triton X-100和 DTT 等）;胶条越长要求上样量越大（考染上样量约为银染上样的5倍）,等电聚焦电压越大,聚焦过程也越长;SDS-PAGE 胶体积分数为10％～15％,常用12％;第二相电泳需选择恒定电流,且先以10 mA ／胶,跑0．5～1．0 h,再以大电流30 mA ／胶至第二相电泳完成． 相似文献
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《实验室研究与探索》2017,(1)
双向电泳技术是当前蛋白质组学研究中的重要技术。设计"双向电泳检测热休克处理对细菌蛋白表达谱的影响"的实验,以PGEX-4T-2大肠杆菌中可溶性总蛋白为研究对象,采用双向电泳技术筛选热休克处理后有差异表达的蛋白。实验获得了稳定性较高、分辨率和重复性较好的电泳图谱,建立了一种适于本科实验教学的双向电泳技术体系。通过该实验,学生可以掌握双向电泳技术的基本原理、实验方法以及蛋白提取的一般思路与方法。 相似文献
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杂交节瓜种子的几种同工酶分析 总被引：1，自引：0，他引：1
肖望 《广东教育学院学报》2001,21(2):71-73
用聚丙烯酰胺凝胶等电聚焦电泳(IEF)方法对杂交节瓜种子的过氧化物酶同工酶(PER)、酸性磷酸酶同工酶(ACP)、酯酶同工酶(EST)进行了电泳分析，发现几种节瓜种子的ACP没有显的差异，EST有一定的差异，而PER具有显的品种间的差异． 相似文献
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本文研究的主要目的是建立大肠杆菌可溶性蛋白质组的双向电泳技术,以等点聚焦为第一向,SDS—PAGE为第二向进行双向电泳,并对样品处理方式、蛋白上样量和染色方法等关键因素和环节进行了比较研究,通过实验条件的筛选和优化获得了较满意的双向电泳图谱。采取的方法具有较高的分辨率和重复性,为进一步研究其蛋白质组学奠定了基础,成功地用于了本科实验教学中。 相似文献
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罗艳萍 《读与写:教育教学刊》2008,5(6)
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity. 相似文献
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Richard Denner 《海外英语》2007,(4):14-15
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose. 相似文献
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Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F. 相似文献
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《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help? 相似文献
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陈美曦 《山东教育学院学报》2008,23(2):103-107
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language. 相似文献
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邱崇丙 《唐山师范学院学报》2014,(1):21-23
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。 相似文献
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《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love. 相似文献