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我科于1994年以来,应用淋病快速诊断试剂盒,对疑似淋病奈瑟氏球菌感染的104例患者进行检查,并用涂片法进行比较,现将结果报告如下: 一、材料和方法 (1)检查对象:104名门诊和临床疑诊为淋病奈瑟氏双球菌感染患者。 相似文献
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建立直接从肉中检测志贺氏菌的快速、敏感、特异的PCR检测方法．根据GenBank公布的志贺氏菌属的侵袭性质粒抗原基因IpaH的保守序列设计特异性引物．经过PCR扩增得到393bp的产物．经过DNA测序证实该产物为目的扩增产物．使用FTA滤膜处理样品．再通过PCR方法检测志贺氏菌．猪肉、牛肉、羊肉检测的检出限均为10CFU／mL匀浆,可在6h内完成对肉中志贺氏菌的检测．FTA滤膜用于PCR检测肉中志贺氏菌灵敏度高,耗时短,为肉中志贺氏菌的快速检测提供了新的方法． 相似文献
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刘北陆 《河北北方学院学报(医学版)》2001,18(2):52-52
近年来,性传播疾病(STD)日益增多,以淋病奈瑟菌(NG)、沙眼衣原体(CT)和解脲脲原体(UU)感染最为多见。本文对我院门诊1996年～2000年拟诊为STD患者1036例,采用PCR方法检测尿道(或宫颈)拭子标本NG、CT和UU,现将结果报告如下。 相似文献
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酶联免疫吸附反应的技术进展及应用 总被引：3，自引：0，他引：3
李文敏 《孝感职业技术学院学报》2003,6(4):65-69
以常规的酶联免疫吸附法为基础，不断改进，形成了多种酶联免疫吸附法，如模块化ELISA，双位点一步法，捕获法测IgM抗体，生物素、亲和素系统，斑点酶链免疫吸附试验，硝酸纤维素膜微粒ELISA等方法。酶链免疫吸附法在医药，食品加工业，农牧渔业等领域有着广泛的应用：如乙型肝炎，莱姆病，急性心肌梗死的早期诊断，定量检测内毒素，HIV抗体初筛，SARS病毒的快速检测；检测食品中的毒素，残留农药，微生物及其它成分；检测香蕉有关病毒，小麦黄花叶病毒，棉花黄萎病菌毒素，转Bt基因抗虫棉，检测水产品中氯霉素的残留量，猪细小病毒(PPV)血清抗体，禽脑脊髓抗体等。 相似文献
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目的：为了证实扁平苔藓特异性抗原的存在．方法：作者对35例扁平苔藓(LP)进行间接免疫荧光(IIF)研究．结果：发现77．14％的病例在皮损处的颓粒层、棘细胞上层显示荧光沉积，但对照组14种其它皮肤病的30例病人也有46．7％的病例在同样部位显示荧光沉积．4例LP特异性抗原(LPSA)阳性的病人治疗前后分别做IIF法检查其结果均为阳性.结论：对以上结果分析，作者认为用IFF法检查不能证实LP有特异性抗原的存在，而所谓的“LPSA”也不能反映病情的变化． 相似文献
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李家明 《四川教育学院学报》2004,20(Z2):187-188
将妇科白带标本涂片后分别作瑞氏、革兰氏以及快速染色检查霉菌、滴虫、加德纳氏菌(线索细胞)、观察清洁度,并与悬滴法比较,结果表明快速染色法查霉菌、滴虫的阳性检出率为35%(85/317)和9%(29/317)与瑞氏和革兰氏染色法无差异,但与悬滴法查霉菌、滴虫的检出率24%( 57/317)和6%(19/317)相比有较明显提高(P<0.01).由于快速染色较其他染色法更具操作简单快速、成本低廉的优点,特别适合门诊和基层医疗单位应用. 相似文献
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为了解大肠埃希氏菌对常用抗生素的敏感性及耐药性,以指导临床选择最有效的抗生素治疗细菌感染,本文应用10种抗生素对从各种标本中分离的349株大肠埃希氏作了药物敏感性测定和分折。结果表明:①大肠埃希氏菌对10种抗生素大都敏感,尤以卡那霉最好,敏感性为66.19％(231株),居各药之首。庆大霉素、四环素、先锋霉素Ⅴ等敏感者均超过50％以上,而对其他部分抗生素已不同程度地产生了耐药。②虽然大肠埃希氏菌对卡那霉素、庆大霉素、四环素等均呈较高的敏感性,但也存在着不同程度的耐药菌株。③各标本间大肠埃希氏菌对药物敏感性经方差分析,349株不同标本的菌株间有显著性差异,F值=52. 53(P<0.01)。大肠埃希氏菌菌株对各种药物的敏感性也有显著性差异,F值=7.07(P<0.01)。因此,常用抗生素在治疗大肠埃希氏菌感染的疾病中仍然是主要的药物。药物敏感减低无疑将会给临床治疗和药物研究工作者带来一定困难。根据上述结果分析,对于大肠埃希氏菌菌株在培养、鉴定之后,选择特定的药敏试验,为指导临床正确并合理地使用抗生素,减少菌株抗药性提供了可靠的依据。 相似文献
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目的：了解我院临床分离出的铜绿假单胞菌感染情况及耐药性,为临床合理应用抗菌药物和医院内感染监控提供依据。方法：对我院2007年1月至2009年12月从临床标本中分离出的铜绿假单胞菌采用肉汤稀释法进行药敏检测,采用WHONET5.3软件进行统计分析。结果：3年共分离出281株铜绿假单胞菌,敏感性较高的分别是亚胺培南（89.4%）、哌拉西林-他唑巴坦（86.5%）、头孢他啶（86.1%）和头孢吡肟（82.8%）,亚胺培南有耐药增加的趋势。其来源主要是痰液和创面分泌物（脓液）标本,科室分布主要以骨科、内科和脑外科为主。结论：铜绿假单胞菌存在较严重的交叉耐药及多重耐药现象。加强对铜绿假单胞菌的耐药性监测,规范抗菌药物的应用,及早进行菌株培养和药敏,是控制铜绿假单胞菌感染和耐药上升的有效措施。 相似文献
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淋球菌孔蛋白对淋球菌的存活和致病起着重要作用。它能促进淋球菌入侵宿主细胞,调控细胞凋亡,影响补体激活途径,充当免疫佐剂。深入研究孔蛋白的免疫功能,对阐明淋球菌的致病机制及宿主抗感染免疫机制具有重要意义。 相似文献
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Keith F. Widaman 《Structural equation modeling》2018,25(6):829-847
Common factor analysis (FA) and principal component analysis (PCA) are commonly used to obtain lower-dimensional representations of matrices of correlations among manifest variables. Whereas some experts argue that differences in results from use of FA and PCA are small and relatively unimportant in empirical studies, the fundamental rationales for the two methods are very different. Here, FA and PCA are contrasted on four key issues: the range of possible dimensional loadings, the range of potential correlations among dimensions, the structure of residual covariances and correlations, and the relation between population parameters and the correlational structures with which they are associated. For decades, experts have emphasized indeterminacies of the FA model, particularly indeterminacy of common factor scores. Determinate in most respects, a heretofore unacknowledged, pernicious indeterminacy of PCA is demonstrated: the indeterminacy between PCA structural representations and the correlational structures from which they are derived. Researchers are often advised to use either FA or PCA in exploratory rounds of data analysis to understand and refine the dimensional structure of a domain before moving to Structural Equation Modeling in later theory-testing, confirmatory, replication studies. Results from the current study suggest that PCA is an unreliable method to use for such purposes and may lead to serious misrepresentation of the structure of a domain. Hence, PCA should never be used if the goal is to understand and represent the latent structure of a domain; only FA techniques should be used for this purpose, as only FA provides reliable structural representations as the basis for confirmatory tests in future studies. 相似文献
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This study examines teacher and student perceptions of formative assessment (FA) activities used by teachers in the classroom. These activities are divided into five phases that together comprise the FA cycle: (1) clarifying expectations, (2) eliciting responses, (3) analysing and interpreting responses, (4) communicating about responses and (5) adjusting teaching and learning. Reliable questionnaires were used to measure the perceptions of 96 teachers and 1,095 students with regard to FA practice. Paired t-tests indicated no differences between the perceptions of teachers and students, except for with regard to clarifying expectations. Teachers used FA activities primarily to clarify expectations and elicit student responses, and they were least likely to apply them to adjust teaching and learning. The results suggest that the framework of the FA cycle could be used as an analytical lens for the reliable evaluation of the FA activities of teachers. 相似文献
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This multiple-case study investigated the experience of three preservice teachers who attempted formative assessment (FA) strategies in their English as a second language writing class during their teaching practicum in a secondary school in Hong Kong. Based on data from 17 lesson observations and 24 interviews, the study examined the three teachers’ implementation of FA strategies during cycles of pre-, while- and post-writing stages. Also analyzed are the teachers’ reflections on and their mentors’ and students’ evaluation of their FA implementation. The study found the teachers could translate their understanding of FA principles into teaching and assessment strategies appropriately, but the school’s accountability culture worked against their innovation. School mentors’ open-mindedness and support to their FA attempts were found to be crucial. The findings are discussed centering on key issues in the relational, developmental and contextual dimensions of the teaching practicum and their impact on preservice teachers’ practice and attitude. 相似文献
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目的：了解HIV性途径感染女性高危人群STD病原体的感染情况,为更好地实施预防治疗及干预活动提供理论基础。方法：采用真菌培养、淋球菌（NG）培养、支原体培养及提取HPV的DNA进行检测。对120例HIV高危人群及73例女性健康体检者泌尿生殖道STD的4种病原体进行检查。结果：HIV高危人群真菌阳性率35．1％,支原体的阳性率74．6％,HPV阳性率为35％。健康体检者真菌阳性率为22．5％,支原体的阳性率52．1％,HPV阳性率为13.3％。两组人群均未分离到淋球菌。结论：除淋球菌外HIV高危人群STD阳性率均高于健康人群,经Х^2检验,支原体及HPV差异有统计学意义。 相似文献
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戊戌文化是生存于维新变法时期,以维新文化为核心内容,以突出救亡图存为主题的,可与当时居统治地位的儒家正统文化相对抗的具有资产阶级文化萌芽性质的一种新文化,属于中国文化由传统到现代的一种转型文化。作为转型文化,它的价值表现在:以西方文化为参照,完成了中国文化由传统的学术价值定位到社会政治价值定位的再造;采用激进的反传统手法,在变法图强的旗帜下推进中国新知识阶层的群体政治意识和权利意识的觉醒。但由于种种因素和原因,戊戌文化也存在着诸如粗陋与肤浅间杂、恋旧与求新并存、过重的尊君色彩等无法克服的缺憾。 相似文献
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This article discusses the opportunity that the Common Core State Standards (CCSS) present for enhancing formative assessment (FA) in history and social studies classrooms. There is evidence that FA can enhance learning for students if implemented well. Unfortunately, teachers continue to be challenged in implementing FA in their classrooms. We examined reading standards for literacy in history and social studies in lesson plans created by preservice teachers in order to discuss opportunities presented by the CCSS for enhancing FA. 相似文献
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Linear factor analysis (FA) models can be reliably tested using test statistics based on residual covariances. We show that the same statistics can be used to reliably test the fit of item response theory (IRT) models for ordinal data (under some conditions). Hence, the fit of an FA model and of an IRT model to the same data set can now be compared. When applied to a binary data set, our experience suggests that IRT and FA models yield similar fits. However, when the data are polytomous ordinal, IRT models yield a better fit because they involve a higher number of parameters. But when fit is assessed using the root mean square error of approximation (RMSEA), similar fits are obtained again. We explain why. These test statistics have little power to distinguish between FA and IRT models; they are unable to detect that linear FA is misspecified when applied to ordinal data generated under an IRT model. 相似文献
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通过对51名调查对象进行血清尿素酶抗体检测，同时进行胃镜检查，采取胃粘膜进行幽门螺杆菌培养，探索出一种更适于临床检测幽门螺杆菌感染的方法。 相似文献