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1.
对存于中山医科大学寄生虫教研室的在定种时使用的8种正副模标本及从虎肺中取出的并殖吸虫标本进行主成分析及聚类分析,结果表明并殖吸虫成虫的6项主要特征中,体形因素及卵巢类型是重要的分类依据。在被分析的9个种中,怡乐村并殖吸虫、斯氏狸殖吸虫、巨睾狸殖吸虫、三平正并殖吸虫、异盘并殖吸虫、陈氏并殖吸虫与卫氏并殖吸虫7个种具有独立地位,而泡囊狸殖吸虫和扁囊并殖吸虫未能分别从斯氏狸殖和卫氏并殖吸虫分开。建立具有独立地位的7种并殖虫吸虫鉴别模型。对中国10个省地区的虫种进行鉴定,结果表明:广东(梅县)、湖南、河南、四川、陕西的吸虫同为斯氏狸殖吸虫;广东(广宁)、江西、浙江、安徽、吉林的并殖吸虫同为卫氏并殖,但各种内不同居群存在形态差别。对16个种的并殖吸虫进行聚类分析,认为16种并殖吸虫可分为两个大群,它们的基本特征符合陈心陶提出的狸殖属与并殖属,但属中所含虫种不尽相同。  相似文献   

2.
二十二年前作者中的一个(Tang,1940)曾从事福建省两种肺吸虫发育史的比较,一种称“林氏型”(Ringeri type)的是卫氏并殖吸虫(Paragonimus westermani),另一种当时称为“鼠型”(Rodent type)的是寄生于本省福清山区沟鼠及野鼠的肺吸虫。在陈心陶教授的怡乐村并殖吸虫专著(Chen,1940)发表之后,福建鼠体的肺吸虫被认为属于怡乐村并殖吸虫(P. iloktsuenensis)而該区“鼠型”的虫种区别的問题便认为已經解决了。近年来国內肺吸虫研究有不少新的发展,在云南,四川,江西以及东北省份新病区的发現,包括新种并殖吸虫的发現要求进一步精  相似文献   

3.
肺吸虫病主要流行于许多发展中国家,是人、兽共患的一种寄生虫病,其病原随地区不同而异。在我国主要为卫氏并殖吸虫(Paragonimus westermani),成虫寄生在人和兽类的肺部,  相似文献   

4.
感染伊氏锥虫(10~5/只)后24小时的NIH小鼠,用不同浓度的DL—a—二氟甲基鸟氨酸(DFMO)水溶液饲喂治疗,连续3天,结果表明,以1%,2%和4%DFMO治疗的小鼠,疗后30天未见虫血症,用其血液及脑组织感染健康的NIH小鼠,也没有出现虫血症,治愈率均达100%。实验证明:DFMO是一种很有发展前途的新型抗锥虫药。  相似文献   

5.
实验选择12头、2-3岁、体重300-500kg雄性去势水牛,经粪便检查和DotELISA检查肝片吸虫阴性,随机分成感染组(n=9)和对照组(n=3),感染组每头一次口服1600个囊蚴.每周定时分别从水牛颈静脉采集感染前0周和感染后25周血液一次,分离血清,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量,旨在研究感染肝片吸虫对水牛机体无机盐含量的影响.结果表明感染后水牛血清中的钾、钠、钙、镁、磷、氯的含量均成波状线变化,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化,这与虫体感染后机体的代谢发生改变有关.  相似文献   

6.
选择 9只 6 - 10月龄的健康雄性白山羊 ,经粪便检查和DotBLISA检测 ,确认无肝片吸虫感染 ,随机分成感染组 (n =5 )和对照组 (n =4 ) ,试验组每只一次口服接种 15 0个肝片吸虫囊蚴。每周定时从颈静脉采集感染前 0周和感染后 14周血液一次 ,分离血清 ,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量 ,旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化 ,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化 ,这与虫体感染后机体的代谢发生改变有关。  相似文献   

7.
在完善的水循环人工生态系统养殖华支睾吸虫的第一、第二中间宿主淡水螺类和鱼类,并进行人工感染实验,为教学提供生物材料。解剖动物收集华支睾吸虫卵,放入培养皿和养殖缸内感染第一中间宿主纹沼螺、长角涵螺;取阳性螺逸尾蚴感染第二中间宿主鱼类;采用鱼肉压片法观察寄生在鱼肉中的活囊蚴;采用人工消化法分离鱼体内的活囊蚴感染实验动物;解剖动物模型。学生观察到了华支睾吸虫生活史发育过程中的各时期活虫体,并获得生态室内鱼类的感染数据。在完善的水循环生态环境条件下,感染华支睾吸虫的第一、二中间宿主生长繁殖良好,其体内的尾蚴和囊蚴为实验教学提供了丰富的生物材料。  相似文献   

8.
目的:评估复方中草药对小鼠抗弓形虫的保护力,以期找到新型防治弓形虫病的药物。创新点:首次在小鼠模型中研究威灵仙、芜荑、白术、刘寄奴和甘草组成的复方中草药的抗弓形虫功效,并发现500 mg的药物能提供较好的保护力。方法:将ICR雌性小鼠随机分为7组:空白组不攻虫不给药;PBS组攻虫;药物对照组攻虫并口服磺胺嘧啶10 mg/d;实验组攻虫并分别灌胃300、400、500和600 mg/d中药煎剂;攻虫组每只小鼠腹腔注射弓形虫RH株1000个。每日观察各组小鼠死亡情况,在感染后3和7 d采集抗凝血检测血常规,并采集血液、肝、脾和肺组织,提取基因组DNA,通过绝对荧光定量PCR检测各组织样品中的虫荷量,采集感染后7 d的肝、肺和脾组织,制作组织切片,通过HE染色观察组织病变情况。结论:试验结果表明,500 mg中药组及磺胺嘧啶药物对照组的小鼠显著地延长了存活时间(图1),且这两组小鼠组织及血液中的虫荷数显著低于其他组(图2),这与小鼠的肝、肺和脾组织病理染色结果一致(图4)。另外,与300、400和600 mg中药处理组相比,500 mg处理组小鼠的单核细胞和淋巴细胞比例均较低(图3),这在一定程度上阻碍了弓形虫在血细胞中的传播。综上所述,500 mg中草药处理组能降低弓形虫的胞内增殖速率,延长小鼠的存活时间,证明该浓度的复方中药具有一定的抗弓形虫效果。  相似文献   

9.
探讨胃癌根治术后腹腔感染对患者远期预后的影响。收集莆田学院附属医院胃肠外科2007年1月至2011年12月入组的胃癌根治患者的临床病理资料,分析胃癌根治术后腹腔感染与预后的关系。结果显示,全组342例患者中,发生腹腔感染患者26例,发生率为7. 6%;腹腔感染死亡患者1例,死亡比率为3. 8%。腹腔感染患者和无感染患者术后5年总体生存率分别为60. 2%和58. 1%(P=0. 089),差异无统计学意义。进一步根据TNM分期,Ⅰ、Ⅱ、Ⅲ期腹腔感染患者的5年总体生存率分别为92. 2%、62. 9%、45. 8%,与无感染组差异均无统计学意义(P>0. 05)。结果表明:胃癌根治术后腹腔感染的发生与术前合并症和长时间手术密切相关,但是腹腔感染并不会降低患者的长期生存率。  相似文献   

10.
选择9只6-10月龄的健康雄性白山羊,经粪便检查和DotBLISA检测,确认无肝片吸虫感染,随机分成感染组(n=5)和对照组(n=4),试验组每只一次口服接种150个肝片吸虫囊蚴。每周定时从颈静静脉集感染前0周和感染后14周血液一次,分离血清,测定血清中的钾、钙、钠、镁、磷、氯等无机盐的含量,旨在研究感染肝片吸虫对山羊机体无机盐含量的影响。结果表明感染后山羊血清中的钾、钠、钙、镁、磷、氯的含量均成波动变化,说明肝片吸虫感染后机体无机盐含量发生了不同程度的变化,这与虫体感染后机体的代谢发生改变有关。  相似文献   

11.
免疫抗毒散为纯中药制剂,经动物试验,其对20日龄罗曼母鸡的最大耐受量在35g/lg体质量以上,灌胃最大耐受量为32g/kg体质量,20%免疫抗毒散小白鼠腹腔LD50=0.3mL/20g体质量,LD50的95%的可信限为0.240~0.376 mL/20g体质量;以20%的浓度给小白鼠和雏鸡连续饮水20d,用药期间小白鼠和雏鸡的精神、食欲、生长发育均正常,剖解时组织器官无任何形态学变化;给孕鼠饮水20d,产仔数和仔鼠的发育与对照组无差异,表明使用免疫抗毒散非常安全。  相似文献   

12.
黄粉甲(Tenebrio moliter)又称黄粉虫、面包虫,是人工养殖最理想的饲料昆虫,温度对黄粉甲的生长和繁殖有明显影响.将黄粉甲在不同的温度条件培养,测定其平均化蛹率;平均死亡率;平均化蛹时间.结果表明黄粉甲最佳培养温度为30℃.  相似文献   

13.
A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus, Zoophthora anhuiensis (Li) Humber, on the green peach aphid, Myzus persicae (Sulzer). Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial dosages (0.4-10.4 conidia/mm2) of Z. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, bioassay 2 was conducted by simultaneously exposing six ≤4-day-old nymphal colonies to a shower of Z. anhuiensis conidia at each of four dosages (resulting from exposures of 0.3-8.0 min). Subsequently, the colonies were separately immersed in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1-12 h after exposure under temperature treatments of 15 and 20℃, respectively. The infection rate during a specific period from the end of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability at a particular dosage. The results showed that the infection of M. persicae from Z. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20℃ before being immersed in the fungicidal solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with the infection rate greatly depending on conidial dosages. It took ≤1 h to infect>50% of the aphids at a dosage of >1.5 conida/mm2 and >90% at >50 conidia/mm2.  相似文献   

14.
Objective: To evaluate the predictive performance of ‘Diprifusor‘ TCI (target-controlled infusion) system for its better application in clinical anesthesia. Methods: The predictive performance of a ‘Diprifusor‘ TCI system was investigated in 27 Chinese patients (16 males and 11 females) during upper abdominal surgery under total intravenous anesthesia (TIVA) with propofol/fentanyl. Measured arterial propofol concentrations were compared with the values predicted by the TCI infusion system.Performance was determined by the median performance error (MDPE), the median absolute performance error (MDAPE), the divergence (the percentage change of the absolute PE with time), and the wobble (the median absolute deviation of each PE from the MDPE). Results: The median (range) values of 14.9% (-21.6%~42.9%) for MDPE, 23.3% (6.9%~62.5%) for MDAPE, -1.9% h^-1(-32.7%~23.0% h^-1) for divergence, and 18.9% (4.2%~59.6%) for wobble were obtained from 227 samples from all patients.For the studied population, the PE did not increase with time but with increasing target propofol concentration, particularly following induction. Conclusions: The control of depth of anaesthesia was good in all patients undergoing upper abdominal surgical operation and the predictive performance of the ‘Diprifusor‘ target controlled infusion system was considered acceptable for clinical purposes. But the relatively bigger wobble showed that the pharmacokinetic model is not so suitable and requires improvement.  相似文献   

15.
本文从气息和听觉两个方面论述单簧管音色训练的方法.气息方面,分别从掌握胸腹式联合呼吸气息要领、气息训练、高音与低音运用气息区别进行论述.听觉方面,通过依靠“头脑”练习、结合不同时期的音乐作品和不同器乐演奏家演奏、结合个人训练与合作训练等方法,培养准确的音准.力度以及音响把握能力.  相似文献   

16.
应用随机分组将600例手术病人两组,每组3000人。一组从手术开始即吸入纯氧,流量4-6升,至麻醉结束为止;另一组术中不给氧。两组术前术后抗生素应用、术中的麻醉方式及用药均基本相同。对两组病人性别、年龄、疾病构成、手术时间、术后肠道功能恢复、伤口感染、白细胞计数、住院时间和费用等多项变量进行统计学处理,两组的感染数、住院时间以及感染病人和非感染病人的白细胞计数、平均住院时间均有显性差异。术中给氧是预防外科伤口感染的使捷方法。  相似文献   

17.
Objective: McKeown esophagectomy followed by cervical and abdominal procedures has been commonly used for invasive esophageal carcinoma. This minimally-invasive operative procedure in the lateral prone position has been considered to be the most appropriate method. We describe our experiences in minimally invasive McKeown esophagectomy (MIME) for esophageal cancer. Methods: Between March 2016 and February 2018, a total of 82 patients underwent MIME by a single group in our department (a single center). All procedure, operation, oncology, and complication data were reviewed. Results: All MIME procedures were completed successfully, with no conversions to open surgery. The median operative time was 260 min, and median blood loss was 100 ml. The average number of total harvested lymph nodes was 20.1 in the chest and 13.5 in the abdomen. There were no deaths within 30 postoperative days. Twenty cases (24.4%) developed postoperative complications, including anastomotic leak in 4 (4.9%), single lateral recurrent nerve palsy in 4 (4.9%), bilateral recurrent nerve palsy in 1 (1.2%), pulmonary problems in 3 (3.7%), chyle leak in 1 (1.2%), and other complications in 7 (including pleural effusions in 4, incomplete ileus in 2, and neck incision infection in 1; 8.54%). Average postoperative hospitalization time was 12 d. Blood loss, operation time, morbidity rate, and the number of harvested lymph nodes were analyzed by evaluating learning curves in different periods. Significant differences were found in operative time (P=0.006), postoperative hospitalization days (P=0.015), total harvested lymph nodes (P=0.003), harvested thoracic lymph nodes (P=0.006), and harvested abdominal lymph nodes (P=0.022) among different periods. Conclusions: Surgical outcomes following MIME for esophageal cancer are safe and acceptable. The MIME procedure for stages I and II could be performed proficiently and reached an experience plateau after approximately 25 cases.  相似文献   

18.
目的:研究人群特异性免疫力对日本血吸虫感染率的影响。方法:选择粪检血吸虫阳性91人(A组)和粪检血吸虫阴性223人(B组)作为研究对象。两组人群经吡喹酮治疗后10d,将观察对象同时暴露在水稻栽插期,栽插期结束后30d用粪检查病,观察两组感染率是否有差异,以此推断特异性免疫力对人群血吸虫感染率的影响。结果:A组感染率(再感染率)为38.46%(35/91),其中6—14岁儿童感染率(再感染率)为10.53%(2/19),15~60岁人群感染率(再感染率)为45.83%(33/72)。B组的感染率为29.60%(66/223),其中6~14岁儿童感染率为20.27%(15/74),15~60岁人群感染率为34.23%(51/149)。结论:人体感染日本血吸虫后产生的特异性免疫力较弱,对降低人群再感染无明显作用。  相似文献   

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