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1.
目的：探讨高原缺氧环境对藏族儿童心脏功能及血流动力学的影响。方法：应用ＨＤＩ－３０００型彩色多普勒血流成像技术检测１００例茂族正常儿童心内血流速度参数及心脏功能、形态。结果：「１」肺动脉峰值流带与主动脉峰值流带基本相等；「２」ＡＴ、ＤＴ：二尖瓣〈三尖瓣；「３」ＰＧＲ：主动态〉肺动脉〉二尖瓣〉三尖瓣；「４」ＬＶＥＴ均值２５０Ｍｓ，ＬＶＭＡＳＳ均值。４１．９５ｇ、ＥＦ６２．０６％、结论：「１」拉萨地区 相似文献
2.
茶多酚抗突变和消除自由基作用的研究 总被引：6，自引：0，他引：6
采用Ｅ．ＣｏｌｉＳＯＳ显色法，小鼠骨髓细胞微核及染色体畸变试验和体外自由基消除实验等方法测定了茶多酚复合物（ＴＰ）的抗突变和消除自由基作用．结果表明：（１）ＴＰ的终浓度在２．５和５ｍｇ／ｍｌ时，对由阳性致突变物ＭＮＮＧ，ＥＭＳ和ＮａＮ＿３诱导的ＳＯＳ应答反应均具有明显的抑制作用；（２）小鼠每日ｉｇ４０、８０和１２０ｍｇ／ｋｇ剂量的ＴＰ能明显抑制由环磷酰胺（ＣＰ）诱发的骨髓ＰＣＥ的ＭＮＣＦ，三种剂量ＴＰ的抑制率分别为４０．９８％、６６．７６％和６５．９４％；（３）ＴＰ对ＭＭＣ诱发的骨髓细胞染色体畸变（ＣＡ）的抑制率分别为６２．５６％、５５．７１％和４７．９５％；（４）在Ｘ／ＸＯ－ＥＳＲ、Ｘ／ＸＯ／Ｌｕｍｉｎｏｌ－ＢＣＬ和ＩＲ／ＮＢＴ－ＳＰＭ三种模型中ＴＰ对超氧阴离子Ｏ＿２自由基均具有极强的消除作用，消除率分别为９８±６％、９８±１．２％和９５±１．５％，对Ｏ＿２的消除效率明显强于维生素Ｃ和Ｅ，对不同工艺制备的ＴＰ消除活性氧自由基作用的测定结果显示：脱咖啡碱程度高，ＥＧＣ和Ｌ－ＥＧＣＧ含量比例高的ＴＰ－３制剂消除Ｏ＿２的效率最高．因此，ＴＰ消除有害自由基，中断或终止自由基的氧化反应是抗突变，抗肿瘤作用的主要机制 相似文献
3.
观察了力平脂对高脂血症者的治疗作用和安全性．确诊高脂血症者６８例，力平脂，０．１，Ｔｉｄ，服一个月为一疗程后，血清总胆固醇（ＴＣ）平均降低２０．９８％，甘油三酯平均降低５１．８８％，ＨＤＬ－Ｃ平均升高１０．２６％，ＬＤＬ－Ｃ平均降低１２．５７％，ＡｐｏＡ１平均升高２．２４％，ＡｐｏＢ平均降低２１．１０％，从调血脂的个体疗效分析，力平脂降低ＴＣ的总有效率８４．６１％，降低ＴＧ的总有效率９５．２４％，升高ＨＤＬ－Ｃ的总有效率６１．７７％，降低ＬＤＬ－Ｃ的总有效率为５５．８８％，升高ＡｐｏＡ１的总有效率３８．２４％，降低ＡｐｏＢ的总有效率７７．９４％．提示：力平脂治疗高脂血症者，对降低ＴＣ，ＴＧ，ＬＤＬ－Ｃ，ＡｐｏＢ和升高ＨＤＬ－Ｃ，ＡｐｏＡ１均有明显效果，其中降低ＴＧ的疗效尤为突出，副作用轻微．临床实践表明：力平脂是一个疗效高、安全、服用方便的调血脂新药 相似文献
4.
自Ｔｓｕｎｏｄａ等对日本山羊碱性磷酸酶（Ｓｃｒｕｍａｌｋａｌｉｎｅ ｐｌｏｓｐｇａｔａｓｅ，ＡＬＰ，Ｅｃ３．１．３）同工酶进行研究，提出有５种同工酶（Ａ，Ｂ，Ｃ，Ｄ，Ｅ）后，有关山羊ＡＬＰ的报道还见于王杰等。Ｔ．Ｓｈｏｔａｋｅ等、郭春华等，认为山羊ＡＬＰＢ具有多态性。本文将叙述柴达木山羊ＡＬＰ同工酶及其多态性的研究结果。１ 材料和方法１．１ 实验动物 随机选取柴达木山羊１２０只作为试验羊。试验羊为终年放牧饲养，放牧地的海拔为２８００－３２００ｍ。１．２ 血样采集 于出牧前从山羊颈静脉… 相似文献
5.
急性有机磷农药中毒（ＡＯＩＰ）病人１６１例，根据入院时病情分三组：Ａ）经典大剂量阿托品组（ＨＤＡＴ）；Ｂ）解磷注射液（简称解磷针）组；Ｃ）解磷加小剂量阿托品组。抢救成功率、轻度三组均为１００％；中度三组分别为９５．５％、９５％、１００％，Ｐ〉０．０５，但ＡＢ二组各死亡１例，原因分别为阿托品过量与不足；重度三组分别为７８．２％、６０．８％、９１．７％，三组比较Ｐ〈０．０５，说明解磷针可单独应用于轻度 相似文献
6.
利用双标图分析基因型和环境交互作用 总被引：28，自引：0，他引：28
在作物的品种区域化试验中，品种的基因型和环境（Ｇ×Ｅ）交互作用越来越受到重视．一种新的非常有效的分析Ｇ×Ｅ交互作用模型是加性主效应和乘积交互作用模型（简称ＡＭＭＩ模型），而双标图（ｂｉｐｌｏｔ）是解释ＡＭＭＩ分析结果的一种直观有效的图形工具．本文对ＡＭＭＩ模型及双标图作一扼要介绍，并选用１９９５年南方水稻区试单季晚粳组数据作为实例，给出了两种新的双标图，以帮助我们更深入地了解Ｇ×Ｅ交互作用． 相似文献
7.
采用聚丙烯酰胺凝胺电泳法对９２头德令哈地区的柴达木黄牛血清淀粉酶同工酶进行了分析。结果发现：（１）柴达木黄牛的血清淀粉酶有ＡＭＹ１,ＡＭＹ２和ＡＭＹ３三种同工酶;（２）ＡＭＹ１同工酶因存在ＡＭＹ１ＢＢ,ＡＭＹ１ＢＣ和ＡＭＹ１ＣＣ三种基因型而表现出多态性;（３）公健牛组与母牛组ＡＭＹ１基因频率和基因型分布没有显著差异。 相似文献
8.
应用聚合酶链反应（ＰＣＲ）检测了１５３例各型乙型肝炎患者的血清ＨＢＶＤＮＡ，并与ＥＬＩＳＡ法检测的血清ＨＢｅＡｇ和抗ＨＢｅ结果比较．结果ＨＢｅＡｇ阳性８９例，ＰＣＲ法ＨＢＶＤＮＡ检出率为９２．１３％；抗ＨＢｅ阳性２７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为５１．８５％；ｅ系统用性３７例，ＰＣＲ法ＨＢＶＤＮＡ检出率为４０．５４％．提示抗ＨＢｅ血清学转换不能作为乙型肝炎病毒复制与非复制、病变活动与静止的唯一指标，而应该用或同时用ＨＢＶＤＮＡ作为指标才可靠． 相似文献
9.
氧化镧对Cu—O／γ—Al2O3催化剂活性和结构的影响 总被引：1，自引：0，他引：1
在色谱－微反流动法实验装置上考察了三种催化剂对ＣＯ和ＣＨ４的氧化活性，并运用ＢＥＴ、ＸＲＤ及ＴＰＲ等技术研究了Ｌａ２Ｏ３的添加对Ｃｕ－Ｏ／γ－Ａｌ２Ｏ３催化剂性能及催化剂氧化还原特性的影响．结果表明，Ｌａ２Ｏ３的添加不仅使Ｌａ２Ｏ３与ＣｕＯ之间产生相互作用，且也与载体γ－Ａｌ２Ｏ３产生相互作用；Ｌａ２Ｏ３的添加促进了ＣｕＯ在γ－Ａｌ２Ｏ３上的分散，并使ＣｕＯ均以非晶相形式存在，从而改善了Ｃｕ－Ｏ／γ－Ａｌ２Ｏ３催化剂对ＣＯ和ＣＨ４的氧化活性，还使Ｌａ－Ｃｕ－Ｏ／γ－Ａｌ２Ｏ３催化剂的ＴＰＲ峰温降低了７５℃． 相似文献
10.
老年期痴呆患者血清中钙镁锌铝含量的研究 总被引：1，自引：0，他引：1
用等离子体直读光谱仪测定了５３例Ａｌｚｈｅｉｍｅｒ型老年痴呆（ＳＤＡＴ）和２５例梗塞性痴呆（ＭＩＤ）患者血清中钙、镁、锌、铝的含量并以４９例同龄的健康老人作为对照,结果显示：ＳＤＡＴ组和ＭＩＤ组血清中钙、锌、铝含量均低于对照组（Ｐ〈０．０１）,而镁在患者组和对照组间无差异,ＳＤＡＴ组与ＭＩＤ组间各元素均无差异,提示这些元素在酶与受体功能调节中起着关键的作用,它们的减少可能是损害中枢神经系统调节功能 相似文献
11.
Detection of lgE and lgG antibodies in Aspergillosis is of diagnostic significance. The serological methods, such as agglutination, gel diffusion and counter immuno electrophoresis that are commonly used in the laboratories for diagnosis of Aspergillus induced infections, are less sensitive and high cross reactivity is often encountered. We carried out work on characterization and identification of diagnostically relevant antigens ofA. fumigatus. Well characterized antigens were used to develop an ELISA with 92% sensitivity and 89% specificity for detection of specific lgE and lgG in the sera of patients of allergic bronchopulmonary aspergillosis (ABPA), aspergilloma and invasive aspergillosis. Subsequently, a sample kit having “ready to use type” of dry reagents (powder/tableted buffers and lyophilized antigen, conjugate and reference sera) was formulated. The kit was validated with sera from patients of ABPA, related allergic disorders, tuberculosis, post-Kochs cases and thalassemic children receiving repeated blood transfusions. The performance of the kit was found to be satisfactory with coded sera. 相似文献
12.
Abolfazl Nasiri Nasrin Ziamajidi Roghayeh Abbasalipourkabir Mohammad Taghi Goodarzi Massoud Saidijam Hamid Behrouj Sara Solemani Asl 《Indian journal of clinical biochemistry : IJCB》2017,32(3):329-336
One of the most important complications of diabetes is nephropathy. This study investigates the effects of aqueous garlic extract on inflammation and oxidative stress status in the kidneys of diabetic rats. Male rats were divided into four groups- control rats, diabetic rats, garlic extract-treated diabetic rats, garlic extract-treated normal rats. The glucose, urea, uric acid, and creatinine levels were measured in sera using colorimetric methods. To determine the oxidative stress condition in the kidney tissues, total antioxidant capacity (TAC), malondialdehyde (MDA), and total oxidant status (TOS) were measured using colorimetric methods. Inflammation status was evaluated by the determination of tumor necrosis factor-alpha (TNF-α) gene and protein expression using qRT-PCR and ELISA respectively, while nitric oxide (NO) level in these tissues was measured using the Griess method. Histological examination of Kidneys was carried out by H&E staining. The levels of glucose, urea, and uric acid were found to increase in the serum of diabetic rats and decrease in that of diabetic rats after treatment with garlic. Measurement of MDA, TOS, and TAC revealed oxidative stress in diabetic rats, which improved after receiving the extract. The NO and TNF-α protein levels in diabetic rats were higher than those in control rats. After treatment with garlic, the levels of TNF-α protein and NO became close to the normal levels. Histological results confirmed certain other data as well. Garlic has antioxidant properties; therefore, it can reduce oxidative stress, which plays an important role in the development of diabetic nephropathy. Reduction in oxidative stress has beneficial effects on inflammation because it leads to a decrease in the level of TNF-α. 相似文献
13.
E. Raji Nair Satish Kumar M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》1998,13(2):98-105
A mycobacterial excretory-secretory protein fraction ESAS-7 purified by 50% ammonium sulphate precipitation followed by SDS-PAGE
fractionation was evaluated by penicillinase enzyme linked immuno-sorbent assay (ELISA) for its sensitivity and specificity
in the diagnosis of pulmonary tuberculosis. At a “cut off” serum dilution of 600, 38 (90%) of 42 sera from bacteriologically
confirmed tuberculosis cases, 15 (100%) of 15 sera from bacteriogically negative but anti tubercular therapy (ATT) responded
cases, 3 (7%) of 43 sera from normal healthy subjects and 4 (8%) of 48 sera from non tuberculous disease control cases gave
positive reaction for tubercular antibody to ESAS-7 antigen fraction containing predominantly 33-kDa protein with a sensitivity
of 90% in bacteriologically confirmed cases and specificity of 92%. Further, this diagnostic assay using the ESAS-7 antigen
is more sensitive requiring as little as one nanogram antigen per test compared to use of 100 nanogram EST-6 antigen reported
earlier. Thus use of ESAS-7 antigen for antibody detection has good diagnostic potential with improved specificity in pulmonary
tuberculosis. 相似文献
14.
本文对伤寒LPS-PHA诊断试剂盒进行现场应用评价.来自各医院和不同地区977份不同类型的血清标本作LPS-PHA检测的同时与Widal试验作比较,结果伤寒沙门氏菌血培养阳性46份血清标本前者检出率(86.96％)显著高于后者(67.39％),两者在统计学上有显著差异(X~2=4.999,P<0.025),95份Widal试验阳性血清LPS-PHA阳性率为97.89％,漏检2份系乙型副伤寒抗体阳性者.607份对照血清标本LPS-PHA的假阳性率1.48％,低于Widal试验(4.84％).伤寒LPS-PHA的敏感度和特异度(94.33％和98.52％)高于Widal试验(89. 36％和95.72％).其他5项评价指标(阳性和阴性预示值,粗一致性,调整一致性和约登指数)亦都较Widal试验好.因此,LPS-PHA诊断试剂盒能满足目前临床上早期快速诊断伤寒的要求.但不适用于追溯诊断和血清流行病学调查. 相似文献
15.
Pradeep Naik S. Indira R. M. Pitchappan T. Malati 《Indian journal of clinical biochemistry : IJCB》1991,6(2):119-123
Antibodies against human leucocyte antigens (HLA) in sera from uni and multiparous women are the potential source of HLA reagent.
The present study was undertaken to screen 169 sera from pregnant women for the presence of HLA antibodies employing 26 panel
cells (Peripheral blood lymphocytes) having known HLA phenotypes. 20.7% (35/169) sera were found to be positive for HLA class-1
antibodies. Present study generated one monospecific, (r=0.6 for A32) the duospecific sera (r=0.5 for A2 B35, r=0.47 A1 DR6
and r=0.7 A28 B51), and rest multispecific sera (r=below 0.4). These positive sera will be utilized as HLA reagents in future
studies for tissue typing. 相似文献
16.
文章实验采用电感耦合等离子体质谱法(ICP-MS)测定广西南宁、北海、桂林、柳州、百色不同地区木薯产地土壤中重金属元素Pb、Cd、As、Hg、Cr、Cu的含量;通过优化消解条件和ICP-MS仪器的分析参数,从而达到有效的检测结果,各元素检出限(ng/ml)分别为:Pb(0.47),Cd(0.083),As(0.13),Hg(0.22),Cr(0.34),Cu(0.22),方法精密度:3.24%~7.24%,加标回收率:95.3%~106.8%;实验方法适用于土壤中Pb、Cd、AS、Hg、Cr、Cu重金属元素含量的检测,为科学的种植提供有效的实验依据。 相似文献
17.
Swati Banerjee E Raji Nair Satish Kumar M. V. R. Reddy Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2001,16(2):203-206
Analysis of tubercular antibody, circulating free and immune complexed antigen (CIC-Ag) was done in confimed pulmonary tuberculosis
sera by ELISA, using ES-31 antigen and affinity purified anti ES-31 antibody. Twenty three of 25 (92%) tuberculosis sera were
positive for IgG antibody to ES-31 antigen. Using anti ES-31 antibody, free tubercular antigen could be detected in 20 of
25 (80%) cases whereas circulating immune complexed antigen (CIC-Ag) in 18 of 25 (72%) cases by sandwich ELISA. Of the two
sera showing absence of antibody, one showed presence of free and CIC-Ag whereas the other showed the presence of free antigen.
Thus antigen assay may be used as an adjunct tool for confirmation of pulmonary tuberculosis. 相似文献
18.
锡渣生产锡酸钠中除锑的研究 总被引：1，自引：0，他引：1
利用生产硫酸亚锡、氯化亚锡、锡粉的含锡废料生产锡酸钠,生产成本低,但在这过程中需要除去的杂质也相对比较多。文章对除锑的工艺参数和条件进行了研究,重点探讨了除锑温度、除锑时间、除锑后溶液静置沉降时间等因素对锑效果的影响。结果表明,采用该工艺,Sb的脱除率为95.3%,产品质量符合Sn-42等级标准。 相似文献
19.
目的了解温州医学院4种期刊论文中统计学应用的状况,并针对存在的问题提出意见和建议。方法选择《温州医学院学报》、《中华眼视光学与视觉科学杂志》、《肝胆胰外科杂志》、《浙江创伤外科》2008-2009年发表的论文进行系统分析,了解其统计学方法的应用情况。结果 4种期刊2008-2009年期间共发表论文2010篇,其中885篇(44.0%)使用了统计学方法。应用的统计学方法包含方差分析443篇(33.2%)、χ2检验387篇(29.0%)、t检验371篇(27.8%)和相关分析84篇(6.3%)等。分析发现应用上的主要问题是统计方法不正确使用、不注明所用的统计学方法和不具体写统计值等。结论作者和编辑人员要充分认识统计学的重要性,学习统计学知识,并加强统计审查。 相似文献
20.
Immunological sensitivity and specificity properties of isolated Plasmodium falciparum (GPL) antigen from culture supernatant
have been measured and compared with malarial antigens and non malarial filtered paper blood sera for potency and efficacy.
Latex bead coded GPL, Pf and RESA antigens immunoreaction properties of human filter paper blood samples (FPB) were studied
by laser light scattering immunoassay (LIA) and Enzyme linked immunoabsorbent assay (ELISA) techniques. Results of GP. antigen
sensitivity study by LIA method showed a very high malaria antibody binding response (MABR) i.e. 6% compared with 78% with
RESA and 88% Pf antigens. Malaria detection by ELISA method also found similar results. Specificity study of GPL antigen for
different non malarial filter paper blood sera (NMFS) showed no immunoreaction however Pf and RESA antigen showed few positive
immunological responses. These results suggest that sensitivity and specificity properties of isolated GPL antigen is better
than other antigens. 相似文献