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1.
以中华芦荟萌蘖芽为外植体,进行了丛生芽的诱导、增殖、生根试验及试管苗的移栽,结果如下:芦荟丛生芽诱导的最佳培养基为MS+6-BA4.0mg.L-1;增殖的较佳培养基为MS+6-BA4.0mg.L-1+NAAO.1mg.L-1,增殖系数为2.1;试管苗生根较好地培养基为MS+NAA0.5mg.L-1和MS+IBA0.5mg.L-1,培养基中加入5g.L-1活性炭不利于根系的生长.  相似文献   

2.
以金边瑞香茎段为外植体,探讨了不同培养基对愈伤组织诱导、愈伤组织分化的影响。结果表明:1)金边瑞香茎段愈伤组织诱导以培养基MS 2,4-D2.0 mg.L-1 6-BA0.2 mg.L-1效果最佳;2)愈伤组织增殖宜采用培养基MS 2,4-D2.0 mg.L-1,并且增殖培养不宜超过1代;3)愈伤组织分化成苗宜采用培养基1/2MS 6-BA2.0 mg.L-1 NAA0.2 mg.L-1,对愈伤组织进行脱水处理能提高分化率。  相似文献   

3.
以青苹果竹芋带节的茎段作为外植体材料进行离体快繁研究,结果表明:芽启动诱导培养基以MS+6-BA(2.00 mg·L-1)+NAA(0.20 mg·L-1)为宜,增殖培养基以MS+6-BA(2.00 mg·L-1)+NAA(0.02 mg·L-1)为宜,青苹果竹芋无需生根培养即可获得较为健壮的小苗,移栽成活率可达95%以上.  相似文献   

4.
通过小白菜的茎尖增殖分化培养、试管苗的生根和移栽培养等试验,研究了小白菜愈伤组织的诱导分化,试管苗的增殖、生根以及移栽条件,并建立了它的无性系。结果表明,不定芽诱导和增殖的最适取材部位为茎尖;最适培养基组成为MS+6-BA2mg/L+NAA0.5mg/L+G30g/L;试管苗生根的最适培养基为:1/2MS+IAA0.2mg/L+G15g/L;试管苗移栽最适培养条件为:散射光下以炉灰渣为基质,并保持湿度为90%。  相似文献   

5.
雷公藤愈伤组织诱导与悬浮培养不定芽诱导   总被引:1,自引:0,他引:1  
以雷公藤茎段为材料,进行了细胞悬浮培养及植株再生诱导的研究.结果表明:愈伤组织诱导培养实验中MS+2,4-D2mg·L-1+KT1.5mg·L-1+NAA0.02 mg·L-1这个处理组中对愈伤组织的诱导培养较为合适,将获得的愈伤组织转置于1/2MS+6-BA1.0 mg·L-1+NAA0.2 mg·L-1液体培养基中,黑暗振荡培养,建立细胞悬浮培养体系,更换6次培养液后,分化出芽:分化芽诱导生根后形成植株.  相似文献   

6.
盾叶薯蓣1、2、4mm茎尖均可诱导出芽,带腋芽幼嫩茎段以MS+BA5mg/L+NAA0.2mg/L培养基诱导率高,增殖培养以BA2mg/L+NAA0.2mg/L培养基增殖率高,生根培养以1/2MS+NAA0.2mg/L培养基生根率最高,移栽成活率在腐叶土及黄沙中均可达90%以上.  相似文献   

7.
以绞股蓝茎尖、茎段、叶片为外植体,研究了不同种类、不同浓度配比的外源激素对绞股蓝愈伤组织诱导的影响。结果表明:茎尖在培养基MS+6- BA 1.0 mg·L-1+NAA 0.02 mg·L-1中的诱导效果最好,其诱导率为75.0%;茎段在培养基MS+6- BA 2.0 mg·L-1+NAA 0.2 mg·L-1中的诱导效果最好,其诱导率为81.8%;叶片在培养基MS+6- BA 1.0 mg·L-1+NAA 0.15 mg·L-1中的诱导效果较好,其诱导率为69.2%。  相似文献   

8.
中华芦荟的组织培养   总被引:4,自引:0,他引:4  
以中华芦荟的芽为外植体进行组织培养,经实验得出中华芦荟组织培养的最佳培养基配方为:1)诱导分化及增殖培养基MS+6-BA3.0mg/L+NAA0.2mg/L;2)生根培养基Ms+NAA0.2mg/L-0.3mg/L.  相似文献   

9.
为了在短期内大量扩繁芦荟优良种苗,满足规格化生产的需要,本文从芦荟外植体的处理、芽体诱导、试管苗的增殖、生根以及试管苗移栽等几方面进行研究,结果表明:升汞处理外植体以8 min效果最好,灭菌成活率达76.8%;茎段在含有6-BA3 mg/L+NAA0.2 mg/L+3%白糖+0.4%琼脂的MS培养基中芽的诱导率达90%;芽体在含有6-BA2mg/L+NAA0.1 mg/L+3%白糖+0.4%琼脂的MS培养基中增殖倍数达6;试管苗在1/2MS+IBA1mg/L+0.3%活性炭+3%白糖+0.4%琼脂中生根率达100%;试管苗移栽基质为河沙或肥沃的沙壤土,移栽成活率达98%以上.  相似文献   

10.
为了在短期内大量扩繁芦荟优良种苗,满足规格化生产的需要,本文从芦荟外植体的处理、芽体诱导、试管苗的增殖、生根以及试管苗移栽等几方面进行研究,结果表明:升汞处理外植体以8 min效果最好,灭菌成活率达76.8%;茎段在含有6-BA3 mg/L+NAA0.2 mg/L+3%白糖+0.4%琼脂的MS培养基中芽的诱导率达90%;芽体在含有6-BA2mg/L+NAA0.1 mg/L+3%白糖+0.4%琼脂的MS培养基中增殖倍数达6;试管苗在1/2MS+IBA1mg/L+0.3%活性炭+3%白糖+0.4%琼脂中生根率达100%;试管苗移栽基质为河沙或肥沃的沙壤土,移栽成活率达98%以上.  相似文献   

11.
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity.  相似文献   

12.
风的曲线     
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose.  相似文献   

13.
Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F.  相似文献   

14.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

15.
一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/  相似文献   

16.
《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?  相似文献   

17.
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language.  相似文献   

18.
汉字倒说     
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。  相似文献   

19.
《海外英语》2007,(4):36
There are numbers of crossroads on our long and unpredictable life journey where we totally have no idea about which direction to choose. No matter what our decision is, we should not turn back, but face the music and go ahead instead. I am this kind of girl who always does try without regretting, one example is how I dealt with my love.  相似文献   

20.
王菲 《华章》2007,(12):273-273
Migration occurs behind a variety of reasons and has a great effect on the whole world. People may migrate in order to improve their economic situation, or in order to escape civil strife, persecution, and environmental disasters. The impact of migration is complex, bringing both benefits anddisadvantages. This paper briefly talks about the causes of migration, the allocation of benefits, and the ways in which individual countries and the international community deal with this important subject.  相似文献   

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