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Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI. 相似文献
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Jun-xian CAO Lu FU Qian-ping GAO Rong-sheng XIE Fan QU 《Journal of Zhejiang University. Science. B》2014,15(6):515-521
Objective
To investigate stretch-induced electrophysiological changes in chronically infarcted hearts and the effect of streptomycin (SM) on these changes in vivo.Methods
Sixty Wistar rats were divided randomly into four groups: a control group (n=15), an SM group (n=15), a myocardial infarction (MI) group (n=15), and an MI+SM group (n=15). Chronic MI was obtained by ligating the left anterior descending branch (LAD) of rat hearts for eight weeks. The in vivo blockade of stretch-activated ion channels (SACs) was achieved by intramuscular injection of SM (180 mg/(kg·d)) for seven days after operation. The hearts were stretched for 5 s by occlusion of the aortic arch. Suction electrodes were placed on the anterior wall of left ventricle to record the monophasic action potential (MAP). The effect of stretching was examined by assessing the 90% monophasic action potential duration (MAPD90), premature ventricular beats (PVBs), and ventricular tachycardia (VT).Results
The MAPD90 decreased during stretching in both the control (from (50.27±5.61) ms to (46.27±4.51) ms, P<0.05) and MI groups (from (65.47±6.38) ms to (57.47±5.76 ms), P<0.01). SM inhibited the decrease in MAPD90 during inflation ((46.27±4.51) ms vs. (49.53±3.52) ms, P<0.05 in normal hearts; (57.47±5.76) ms vs. (61.87±5.33) ms, P<0.05 in MI hearts). The occurrence of PVBs and VT in the MI group increased compared with that in the control group (PVB: 7.93±1.66 vs. 1.80±0.86, P<0.01; VT: 7 vs. 1, P<0.05). SM decreased the occurrence of PVBs in both normal and MI hearts (0.93±0.59 vs. 1.80±0.86 in normal hearts, P<0.05; 5.40±1.18 vs. 7.93±1.66 in MI hearts, P<0.01).Conclusions
Stretch-induced MAPD90 changes and arrhythmias were observed in chronically infarcted myocardium. The use of SM in vivo decreased the incidence of PVBs but not of VT. This suggests that SACs may be involved in mechanoelectric feedback (MEF), but that there might be other mechanisms involved in causing VT in chronic MI. 相似文献4.
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Carl A. Blashko John G. Paterson 《International journal for the advancement of counseling》1990,13(1):49-59
This article is focussed on a heart disease prevention program which used a mass media approach to facilitate changes in lifestyle of the population. A radio station, 930 CJCA, in Edmonton, Alberta, Canada aired five consecutive 1 1/2 hour open-line programs in which several aspects of heart disease were addressed by health experts. A public health meeting was held afterwards. Approximately 500 persons attended, 430 of whom filled out questionnaires and were interviewed by telephone six months later. Over half of the respondents made substantive lifestyle changes to reduce heart disease; approximately one-quarter had checked for evidence of diabetes; two-thirds had their blood pressure checked within the six month follow-up period. People in this group seemed to exercise more and smoke less. The importance of utilizing the mass media to alter lifestyles of the public is documented. Most authors agree that primary prevention programs are needed to enhance the general health of the population. 相似文献
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《中学生读写(初中)》2010,(3)
传递小纸条的方法认真填写读者调查表,同时写下你对生活、对朋友想说的话,寄回爱小说编辑部。亲爱的i迷们.请用最特别的方式传递自己的心声吧!徐洁,你一定要幸福:陈文,你一定要幸福——我们所有人都一定要幸福,很幸福。河北邯郸徐安安我愿意为美好的未来而努力,我愿意和善意的人们去交谈,我愿意积极地去生活——让一切重新开始吧。湖南长沙陈愿 相似文献
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王丽霞 《湖北广播电视大学学报》2009,29(8):82-83
祥林嫂心中的死结是由一对对的矛盾构成的。解开祥林嫂无法解开的结,就能深刻地领会文章的主旨,就能进一步揭露封建礼教的欺骗性、虚伪性和残酷性,有力地抨击中国传统文化在民族心理性格方面形成的种种病态,进而唤醒民众的觉悟。 相似文献
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李志杰 《宁波职业技术学院学报》2010,14(5):26-28
介绍一种脉搏智能控制系统的设计方案。以单片机（AT89C51）为核心器件实现对系统进行自动控制,采用脉搏传感器对人体的脉搏进行实时的检测,通过复位按钮和LED七段码提供显示来实现人机操作界面。该系统能够及时显示采集到的数据值,并进行处理,从而引入了脉搏智能控制系统的新概念。 相似文献
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Human bone marrow-derived mesenchymal stem cells transplanted into damaged rabbit heart to improve heart function 总被引：18，自引：0，他引：18
INTRODUCTION Congestive heart failure is the end stage of manycardiovascular diseases. Myocardial infarction (MI)is a life-threatening event that may cause suddencardiac death and heart failure. Despite considerableadvances in diagnosis and treatment of heart disease,cardiac dysfunction after MI is still the majorworldwide cardiovascular disorder. Damaged myo-cardium after acute MI is gradually replaced by fi-brotic noncontractile cells to form scar tissue. Thedeveloping ventricul… 相似文献
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Cell therapy in congestive heart failure 总被引：5，自引：0，他引：5
Congestive heart failure （CHF） has emerged as a major worldwide epidemic and its main causes seem to be the aging of the population and the survival of patients with post-myocardial infarction. Cardiomyocyte dropout （necrosis and apoptosis） plays a critical role in the progress of CHF; thus treatment of CHF by exogenous cell implantation will be a promising medical approach. In the acute phase of cardiac damage cardiac stem cells （CSCs） within the heart divide symmetrically and/or asymmetrically in response to the change of heart homeostasis, and at the same time homing of bone marrow stem cells （BMCs） to injured area is thought to occur, which not only reconstitutes CSC population to normal levels but also repairs the heart by differentiation into cardiac tissue. So far, basic studies by using potential sources such as BMCs and CSCs to treat animat CHF have shown improved ventricular remodelling and heart function. Recently, however, a few of randomized, double-blind, placebo-controlled clinical trials demonstrated mixed results in heart failure with BMC therapy during acute myocardial infarction. 相似文献
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Although the management of chronic heart failure (CHF) has made enormous progress over the past decades, CHF is still a tremendous medical and societal burden. Metabolic remodeling might play a crucial role in the pathophysiology of CHF. The characteristics and mechanisms of metabolic remodeling remained unclear, and the main hypothesis might include the changes in the availability of metabolic substrate and the decline of metabolic capability. In the early phases of the disease, metabolism shifts toward carbohydrate utilization from fatty acids (FAs) oxidation. Along with the progress of the disease, the increasing level of the hyperadrenergic state and insulin resistance cause the changes that shift back to a greater FA uptake and oxidation. In addition, a growing body of experimental and clinical evidence suggests that the improvement in the metabolic capability is likely to be more significant than the selection of the substrate. 相似文献