共查询到20条相似文献，搜索用时 31 毫秒
1.
Objective: to explore a new serological method for detecting Helicobac ter pylori ( H. pylori ) infection. Methods: Serum soluble antigen of H. p ylor i was detected by using avidin-biotin ELISA technique to evaluate the status of H. pylori infection and for comparison with rapid urease test ( RUT ), histo logi c examination and serology. Results: The sensitivity, specificity, positive pred ictive value and negative predictive value were 77.46%, 91.07%, 91.67% a nd 76.12 %, respectively. The prevalence rate of serum H. pylori soluble antigen in 138 patients undergoing endoscopy was similar to the rate obtained by 14 C-UBT met hods ( P>0.05 ). Conclusions: The detection of serum H. pylori solub le antigen( HpSAg) could be used as a new serological method which is accurate, and convenie nt, not affected by the memorizing reaction of serum antibody; is more sensitive , m ore specific and suitable for clinical diagnosis, and evaluation of eradication and for follow-up of H. pylori as well as for detection in children and pre gnant women. 相似文献
2.
Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) andHelicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with
chronic gastric diseases were detected by ELISA method and the status ofH. pylori was determined by histologic examination, RUT,14C-UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1
were significantly higher in patients withH. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml,P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection ofH. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml, respectively (P<0.05). The serum levels of sICAM-1, proved to be significantly correlated with the density ofH. pylori colonization in gastric mucosa (r
s=0.316,P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those
in healthy controls (P<0.05). Conclusions: These results indicated thatH. pylori infection up-regulates the expression of sICAM-1. 相似文献
3.
Objective: To investigate the distribution ofH. pylori antigens in the gastric mucosa in patients withH. pylori infection, and the relationship between the distribution and gastric cancer. Methods: Of 112 patients confirmed by pathological
study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical
hyperplasia) and gastric cancer, 28 wereH. pylori negative and 84 wereH. pylori positive.H. pylori antigens in the gastric mucosa were detected by immunohistochemistry. Results: TheH. pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous
changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates ofH. pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12), 63.6% (14/22) and 84.6% (11/13) for the same
groups (χ
2=19.76,P=0.000);H. pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial
gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the
isthmus in 9 of 13 (69.2%) cases with gastric cancer (χ
2=25.30,P=0.000). In theH. pylori negative group, noH. pylori antigen was observed. Conclusion: With the progression of chronic superficial gastritis→precancerous changes→gastric cancer,H. pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric
mucosa. 相似文献
4.
Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein ofH. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases.
Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion
from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic
ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western-blot using
the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric
point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44),
atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7), gastric cancer 100% (2/2). Compared with each other, the
difference was significant (χ2=13.327,P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (r
s=0.442,P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases.
Project supported by the China Medical Board (96-628) and Zhejiang Province Hygiene Bureau (2000 A055) 相似文献
5.
The relationship between ulcer recurrence andHelicobacter pylori: A prospective one-year follow-up study in China 总被引：1，自引：0，他引：1
To study the relationship between ulcer recurrence andHelicobacter Pylori. 147 patients with peptic ulcer were divided intoH. Pylori infected group andH. pylori uninfected group by rapid urease test serum antibody examination and histopathology of gastric biopsy specimens. We assigned
all patients to receive dual oral therapy with omeprazole 40 mg/day for 4 weeks and amoxicillin 2000 mg/day for 2 weeks. To
all patients, repeated gastroscope was performed at 8 weeks and one-year after beginning therapy to record whether the ulcer
was cured. Endoscopic biopsy was also performed to determineH. pylori status. One year later,H. pylori positive patients had a higher ulcer recurrent rate thanH. pylori negative patients (23. 33% vs 2. 70%; P=0.001). Ulcer recurrence was closely related toH. pylori infection.H. pylori was the risk factor for ulcer relapse. 相似文献
6.
Objective: To determine the existence of genus-specific antigens in outer membrane proteins (OMPs) ofleptospira with different virulence. Methods: Microscope agglutination test (MAT) was applied to detect the agglutination between commercial
rabbit antiserum against leptospiral genus-specific TR/Patoc I antigen and 17 strains ofLeptospira interrongans belonging to 15 serogroups and 2 strains ofLeptospira biflexa belonging to 2 serogroups. The outer envelopes (OEs) ofLinterrogans serogroupIcterohaemorrhagiae serovarlai strainlai (56601) with strong virulence and serogroupPomona serovarpomona strainLuo (56608) with low virulence, andL. biflexa serogroupSemaranga serovarpatoc strainPatoc I without virulence were prepared by using the method reported in Auranet al. (1972). OMPs in the OEs were obtained by treatment with sodium deoxycholate. SDS-PAGE and western blot were used for analyzing
the features of the OMPs on electrophoretic pattern and the immunoreactivity to the antiserum against TR/Patoc I antigen,
respectively. Results: All the tested strains belonging to different leptospiral serogroups agglutinated to the antiserum
against leptospiral genus-specific TR/Patoc I antigen with agglutination titers ranging from 1:256–1:512. A similar SDS-PAGE
pattern of the OMPs from the three strains ofleptospira with different virulence was shown and the molecular weight of a major protein fragment in the OMPs was found to be approximately
60 Kda. A positive protein fragment with approximately 32 KDa confirmed by Western blot, was able to react with the antiserum
against leptospiral genus-specific TR/Patoc I antigen, and was found in each the OMPs of the three stains ofleptospira. Conclusion: There are genus-specific antigens on the surface ofL.interrogans andL.biflexa. The OMP with molecular weight of 32 KDa may be one of the genus-specific protein antigens ofleptospira.
Project (No. 39970678) supported by the National Natural Science Foundation of China 相似文献
7.
A two-step method was developed to quantitatively assess the infection rate of the entomophthoraceous fungus,Zoophthora anhuiensis (Li) Humber, on the green peach aphid,Myzus persicae (Sulzer). Firstly, a standard time-dose-mortality relationship, established by modeling data from bioassay 1 at varying conidial
dosages (0.4–10.4 conidia/mm2) ofZ. anhuiensis F97028, was used to yield an estimate of expected mortality probability at a given dosage. Secondly, biossay 2 was conducted
by simultaneously exposing six ≤4-day-old nymphal colonies to a shower ofZ. anhueinsis conidia at each of four dosages (resulting from exposures of 0.3–8.0 min). Subsequently, the colonies were separately immersed
in a 0.1% chlorothalonil solution for 0.5 min to disinfect all surviving conidia on the host integument from 1–12 h after
exposure under temperature treatments of 15 and 20°C, respectively. The infection rate during a specific period from the end
of the exposure to the immersion was then estimated as the ratio of the observed mortality over the expected mortality probability
at a particular dosage. The results showed that the infection ofM. persicae fromZ. anhuiensis was highly rapid with little difference between aphid colonies maintained at 15 and 20°C before being immersed in the fungicidal
solution after exposure. The first 6-hour period after exposure was most crucial to successful infection of the fungus with
the infection rate greatly depending on conidial dosages. It took ≤1 h to infect >50% of the aphids at a dosage of >1.5 conida/mm2 and >90% at >50 conidia/mm2.
Project supported by the National Natural Science Foundation of China (No. 39870513) and the Cheong Kong Scholars Programme,
State Education Ministry of China 相似文献
8.
Strain improvement and medium optimization to increase the productivity of spiramycin were carried out. Of oil tolerant mutant
strains screened, one mutant,Streptomyces ambofaciens XC 2–37, produced 9% more spiramycin than the parent strainS. ambofaciens XC 1–29. The effects of soybean oil and propyl alcohol on spiramycin production withS. ambofaciens XC 2–37 were studied. The potency ofS. ambofaciens XC 2–37 was improved by 61.8% with addition of 2% soybean oil in the fermentation medium and 0.4% propyl alcohol at 24 hours
after incubation. The suitable time for feeding propyl alcohol is at 24 hours after incubation in flask fermentation and at
20 hours after incubation in fermentor fermentation. The new process withS. ambofaciens XC 2–37 was scaled up for industrial scale production of spiramycin in a 60 m3 fermentor in Xinchang Pharmaceutical Factory, Zhejiang Medicine Company, Ltd., China, and the potency and productivity of
fermentation were improved by 42.9%. 相似文献
9.
Yan-yan Shi Jing Zhang Ting Zhang Man Zhou Ye Wang He-jun Zhang Shi-gang Ding 《Journal of Zhejiang University. Science. B》2018,19(10):750-763
Helicobacter pylori infection is related to the development of gastric diseases. Our previous studies showed that high thioredoxin-1 (Trx1) expression in H. pylori can promote gastric carcinogenesis. To explore the underlying molecular mechanisms, we performed an isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomic analysis of stomach tissues from Mongolian gerbil infected with H. pylori expressing high and low Trx1. Differences in the profiles of the expressed proteins were analyzed by bioinformatics and verified using Western blot analysis. We found three candidate proteins, 14-3-3α/β, glutathione-S-transferase (GST), and heat shock protein 70 (HSP70), in high Trx1 tissues compared with low Trx1 tissues and concluded that cellular stress and redox activityrelated proteins were involved in the pathogenesis of gastric cancer associated with H. pylori Trx1. 相似文献
10.
Insertion mutagenesis has become one of the most popular methods for gene functions analysis. Here we report a two-elementAc/Ds transposon system containing enhancer trap and gene trap for gene tagging in rice. The excision ofDs element was examined by PCR amplification. The excision frequency ofDs element varied from 0% to 40% among 20 F2 populations derived from 11 differentDs parents. Southern blot analysis revealed that more than 70% of excisedDs elements reinserted into rice genome and above 70% of the reinsertedDs elements were located at different positions of the chromosome in rice. The result of histochemical GUS analysis indicated
that 28% of enhancer trap and 22% of gene trap tagging plants displayed GUS activity in leaves, roots, flowers or seeds. The
GUS positive lines will be useful for identifying gene function in rice.
Project supported by the Hi-Tech Research and Development Program (863) of China (No. 2002AA2Z1003) 相似文献
11.
Zheng Hu-zhe Cui Chun-lan Zhang Yu-ting Wang Dan Jing Yu Kim Kil Yong 《Journal of Zhejiang University. Science. B》2005,6(8):778-786
The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus
ofGlomus intraradices and/or infection with pathogenic strain ofPhytophthora capsici, and the biological control effect ofG. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated
withG. intraradices (Gi), (2) plants pre-inoculated withG. intraradices and then infected withP. capsici (Gi+Pc). (3) plants infected withP. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in
pathogen challenged plants. Root mortality caused by infection ofP. capsici was completely eliminated by pre-inoculation with antagonisticG. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only
21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase
(PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased
in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9%
in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major
POD isozymes (45000, 53000 and 114000) were present in Pc-treated roots, while two major bands (53000 and 114000) and one
minor band (45000) were present in spectra of Gi+Pc-treated roots, the 45000 POD isozyme was significantly suppressed byG. intraradices, suggesting that the 45000 POD isozyme was induced by the pathogen infection but not induced by the antagonisticG. intraradices. A 60000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the
inoculation of antagonisticG. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper
plants infected byP. capsici. The results suggested thatG. intraradices is a potentially effective protection agent againstP. capsici.
Project supported by Korea Science and Engineering Foundation (KOSEF) through the Agricultural Plants Stress Research Center
(APSRC) at Chonnam National University, Korea 相似文献
12.
INTRODUCTION Helicobacter pylori (H.pylori) was grouped as a class I carcinogen by the International Agency for Research on Cancer in 1994. A direct relation be-tween H.pylori infection and gastric carcinogenesis was demonstrated in 1998 in an experimental animal model (Watanabe et al., 1998). However, the role of H.pylori in human gastric carcinogenesis is sup-ported almost exclusively by epidemiological data and prospective histopathological studies. So far the mechanism of H.pylor… 相似文献
13.
Objective: To investigate the distribution of H. pylori antigens in the gastric mucosa in patients with H. pylori infection, and the relationship between the distribution and gastric cancer. Methods: Of 112 patients confirmed by pathological study to have chronic superficial gastritis, precancerous changes (chronic atrophic gastritis, intestinal metaplasia or atypical hyperplasia) and gastric cancer, 28 were H. pylori negative and 84 were H. pylori positive. H. pylori antigens in the gastric mucosa were detected by immunohistochemistry. Results: The H. pylori positive group, comprised 12 of 22 (50.0%) in the chronic superficial gastritis group, 22 of 25 (88.0%) in the precancerous changes group and 13 of 35 (37.1%) in the gastric cancer group. The positive rates of H. pylori antigens in the cytoplasm progressively increased, respectively at 0.0% (0/12),63.6% (14/22) and 84.6% (11/13) for the same groups (χ2=19.76, P=0.000); H.pylori antigens were located in the mucus layer and above the neck of the mucosal gland in 9 of 12 (75.0%) cases with chronic superficial gastritis, at the neck of the mucosal gland and the isthmus in 12 of 22 (54.5%) cases with precancerous changes, below the isthmus in 9 of 13 (69.2%) cases with gastric cancer (x2=25.30, P=0.000). In the H. pylori negative group, no H.pylori antigen was observed. Conclusion: With the progression of chronic superficial gastritis→precancerous changes→gastric cancer, H. pylori antigens progressively migrated from the outer part to the inner part of the cell, and from the superficial to the deep gastric mucosa. 相似文献
14.
Objective: To evaluate the interaction between serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) and Helicobacter pylori (H. pylori) infection in patients with chronic gastritis and peptic ulcer. Methods: The serum levels of sICAM-1 in 205 patients with chronic gastric diseases were detected by ELISA method and the status of H. pylori was determined by histologic examination, RUT, 14C - UBT, and serology. The sera obtained from 18 healthy volunteers served as controls. Results: The serum levels of sICAM-1 were significantly higher in patients with H. pylori positive than those of H. pylori negative (889.43±32.52 ng/ml vs. 747.07±30.45 ng/ml, P<0.05). The serum levels of sICAM-1 in patients with mild, moderate and severe infection of H. pylori were 841.68±72.36 ng/ml, 905.43±37.59 ng/ml and 1012.54±49.34 ng/ml,respectively (P<0.05). The serum levels of sICAM-1 proved to be significantly correlated with the density of H. pylori colonization in gastric mucosa (rs =0.316, P<0.001). The serum levels of sICAM-1 in patients with chronic gastritis and peptic ulcer were significantly higher than those in healthy controls (P<0.05). Conclusions: These results indicated that H. pylori infection up-regulates the expression of sICAM-1. 相似文献
15.
Bradley T. Erford Donna L. Bagley James A. Hopper Ramona M. Lee Kathleen A. Panagopulos Denise B. Preller 《Psychology in the schools》1998,35(2):127-135
The Math Essential Skill Screener–Elementary Version (MESS-E) is a screener devised to identify primary grade students at risk for math difficulties. Item analysis, interitem consistency, test–retest reliability, decision efficiency, and construct validity of the MESS-E were studied using four independent samples of boys and girls grades 1–3 (aged 6–8). Item analysis revealed median item difficulty of .64 and median item discrimination of .75. Interitem consistency was .92 (n = 171) and .94 (n = 711), while 30-day test–retest reliability was .86 (n = 125). Exploratory factor analysis indicated a one-factor solution accounting for 37% of observed variance. LISREL 7 confirmatory factor analysis procedures determined that the one-factor model fit the standardization sample data poorly (goodness-of-fit index = .729, χ2 to df ratio = 9.91). The MESS-E yielded concurrent validity coefficients (n = 171) of .74 with the Woodcock–Johnson: Tests of Achievement–Revised (WJ-R) Math Cluster, .80 with the Wide-Range Achievement Test–Revised (WRAT-R) Arithmetic subtest and .73 with the KeyMath-R Operations Area standard scores. A diagnostic efficiency study yielded a total predictive value (TPV) of .93, sensitivity = .98, specificity = .88, positive predictive power (PPP) = .89, negative predictive power (NPP) = .98, and incremental validity = 39%. The MESS-E displayed a slight tendency to overidentify children potentially at risk for math difficulties. © 1998 John Wiley & Sons, Inc. 相似文献
16.
Study of the morphology, aggregation structure and properties ofBombyx mori silk treated by low temperature oxygen plasma showed that slight flutes appeared on the surface ofBombyx mori silk fiber and that its surface structure changed after plasma treatment. The conformation also changed and crystalline degree
decreased. The stannic filling rate of treated fiber was improved. Because of etching, the weight of the fiber decreased but
the breaking strength changed little after short-time treatment.
Project (No. 50073013) supported by the National Natural Science Foundation of China 相似文献
17.
Objective: To observe the effect of Yangxueqingnao particles on rat vascular smooth muscle cell (VSMC) proliferation induced
by lysophosphatidic acid (LPA). Methods: The amount of3H-TdR (3H-thymidine) admixed in cultured rat VSMC was measured and mitogen-activated protein kinase (MAPK) activity and lipid peroxidation
end product malondialdehyde (MDA) content of the VSMC were assayed. Results: 1×10−9, 1×10−8, 1×10−7 mol/L LPA in a concentration dependent manner, induced the amount of3H-TdR admixed, MAP kinase activity, and MDA content of the cultured rat VSMC to increase. However, 5%, 10%, and 15% Yangxueqingnao
serum preincubation resulted in a decrease of 23.0%, 42.0%, and 52.0% (P<0.01) respectively in the amount of3H-TdR admixed, a decline in VSMC MAP kinase activity of 13.9% (P<0.05), 29.6% (P<0.01), and 48.9% (P<0.01) respectively, and also, a decrease in MDA content of VSMC of 19.4%, 24.7%, and 43.2% (P<0.01) respectively, in the 1×10−7 mol/L LPA-treated VSMC. Conclusions: LPA activates the proliferation and lipid peroxidation of VSMC in a concentration dependent
manner. The LPA-induced VSMC proliferation is related to the activity of MAP kinases, enzymes involved in an intracellular
signalling pathway. The results of the present study showed that Yangxueqingnao particles can effectively inhibit LPA-induced
VSMC proliferation, MAP kinase activation, and reduce lipid peroxidative lesion.
Project (No. 491010-W50339) supported by Chinese Traditional Medicine Administration Bureau of Zhejiang Province, China 相似文献
18.
Leonardo Martín Pérez Francesc Codony Daniela López Leyton Mariana Fittipaldi Bárbara Adrados Jordi Morató 《Journal of Zhejiang University. Science. B》2010,11(1):27-29
Helicobacter pylori are ubiquitous Gram-negative bacteria with a high estimated level of infection in the world populations, but a majority of
the infected persons are asymptomatic. This pathogen has been classified by the World Health Organization as a class I carcinogen
and recognized as the causal agent of most peptic ulcers and chronic gastritis that might lead to stomach cancer. Although
not all the transmission pathways of these bacteria into humans have been properly identified, enough data have suggested
that the oral-oral or fecal-oral ones are the main infection routes. Helicobacter pylori have been detected in non-treated water and in drinking water, which suggested that water might be an important infection
source. As childhood is the critical period of infection, the aim of the present work was to examine the presence of Helicobacter pylori in soil samples from public playing areas of Spanish parks. 相似文献
19.
20.
Rui Chen Shao-hong Lu Qun-bo Tong Di Lou Dong-yan Shi Bing-bing Jia Guo-ping Huang Jin-fu Wang 《Journal of Zhejiang University. Science. B》2009,10(7):512-521
The dense granule protein 4 (GRA4) is a granular protein from Toxoplasma gondii, and is a candidate for vaccination against this parasite. In this study, the plasmid pcDNA3.1-GRA4 (pGRA4), encoding for
the GRA4 antigen, was incorporated by the dehydration-rehydration method into liposomes composed of 16 mmol/L egg phosphatidylcholine
(PC), 8 mmol/L dioleoyl phosphatidylethanolamine (DOPE), and 4 mmol/L 1,2-diodeoyl-3-(trimethylammonium) propane (DOTAP).
C57BL/6 mice and BALB/c mice were immunized intramuscularly three times with liposome-encapsulated pGRA4 to determine whether
DNA immunization could elicit a protective immune response to T. gondii. Enzyme-linked immunosorbent assay (ELISA) of sera from immunized mice showed that liposome-encapsulated pGRA4 generated
high levels of IgG antibodies to GRA4. Production of primary interferon (IFN)-γ and interleukin (IL)-2 in GRA4-stimulated
splenocytes from vaccinated mice suggested a modulated Th1-type response. 72.7% of C57BL/6 mice immunized with liposome-encapsulated
pGRA4 survived the challenge with 80 tissue cysts of ME49 strain, whereas C57BL/6 mice immunized with pGRA4 had only a survival
rate of 54.5%. When immunized BALB/c mice were intraperitoneally challenged with 103 tachyzoites of the highly virulent RH strain, the survival time of mice immunized with liposome-encapsulated pGRA4 was markedly
longer than that of other groups. Our observations show that liposome-encapsulated pGRA4 enhanced the protective effect against
infection of T. gondii.
Project supported by the Science Foundation of the Health Bureau of Zhejiang Province, China (Nos. 2003QN003 and 2005A001)
and the Science Foundation of the Science and Technology Department of Zhejiang Province, China (No. 2006C13022) 相似文献