共查询到19条相似文献，搜索用时 109 毫秒
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李宏 《重庆第二师范学院学报》2002,15(3):64-66
传统的蛋白质结构模型要首先将蛋白质纯化并结晶，再进行X－射线衍射和核磁共振分析才能得出；结构基因组学应用基因和基因组信息，通过计算机程序建立蛋白质结构模型，其速度快，精确度高。利用建立的蛋白质模型，可以有目的地合成人类需要的药物和推动生物学理论研究。本介绍了建立蛋白质结构的同源模型计算机模拟技术。 相似文献
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基因组学是研究生物基因组结构与功能、基因组网络及其相互作用的遗传学分支学科,涉及基因作图、测序和整个基因组功能分析,提供基因组信息以及相关数据系统利用,试图解决生物、医学、工业领域的重大问题。近年来,基因组学技术在生物多样性保护研究中取得了令人瞩目的应用成效。文章在概述基因组学技术类型的基础上,综合分析了基因组学技术在种群遗传学研究、物种鉴定和保护、克隆与保护濒危物种和生态系统功能稳定性研究中的应用状况,并针对基因组学技术在生物多样性保护研究中面临的挑战,提出了若干针对性应对建议。 相似文献
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植物基因组的研究已经由以全基因组测序为目标的结构基因组学转向以基因功能鉴定为目标的功能基因组学研究.植物功能基因组学研究是利用结构基因组学积累的数据,从中得到有价值的信息,阐述DNA序列的功能,从而对所有基因如何行使其职能并控制各种生命现象的问题作出回答.近年来植物功能基因组学的研究技术主要包括表达序列标签、基因表达的系列分析、DNA微阵列和反向遗传学等.对植物功能基因组学的研究将有利于我们对基因功能的理解和对植物形状的定性改造和利用. 相似文献
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木质素结构在聚丙烯中抗氧化作用的影响 总被引：1，自引：0，他引：1
采用氧化诱导温度测试方法,研究了添加0.2%～1.0%桦木木质素、松木木质素和玉米秆木质素的聚丙烯的抗氧化性能。结果表明,3种结构不同的木质素都有一定的抗氧化效果,其中针叶材木质素的抗氧化效果最好。通过红外光谱和核磁共振氢谱分析了各种木质素的结构,发现针叶材木质素中含有大量的愈创木基丙烷结构,而没有紫丁香基丙烷结构的存在;阔叶材木质素和草本木质素中既有愈创木基丙烷结构也有紫丁香基丙烷结构,从结构上解释了来源不同的木质素抗氧化效果不同的原因。结合不同木质素样品的红外光谱和核磁共振氢谱分析得知,羟基对木质素的抗氧化性有较大贡献。 相似文献
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本文介绍了转座子的基本结构和转座子标签技术分离基因的基本原理、方法;并就转座子标签技术的研究新进展,转座子标签技术在水稻功能基因组学研究中的应用等方面进行了综述. 相似文献
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功能基因组学研究方法 总被引：5，自引：0，他引：5
随着后基因组时代的到来，基因组学的研究从结构基因组学转向了功能基因组学，基因功能的研究也从单一基因转向了大规模、批量分析。本文对功能基因组学研究方法进行了综述，主要包括：微阵列分析、基因表达系列分析、基因剔除和转基因技术、RNA 干涉、蛋白质组学和生物信息学等新方法。 相似文献
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由于基因组计划的进展 ,基因组学的研究已从结构基因组学转向功能基因组学。文章介绍了几种大规模分析基因功能的新技术和新方法 ,包括微点阵、基因表达系列分析 (SAGE)、蛋白质组、生物信息学等 相似文献
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Ying Zhang Rajini Haraksingh Fabian Grubert Alexej Abyzov Mark Gerstein Sherman Weissman Alexander E. Urban 《Child development》2013,84(1):34-48
Structural variation of the human genome sequence is the insertion, deletion, or rearrangement of stretches of DNA sequence sized from around 1,000 to millions of base pairs. Over the past few years, structural variation has been shown to be far more common in human genomes than previously thought. Very little is currently known about the effects of structural variation on normal child development, but such effects could be of considerable significance. This review provides an overview of the phenomenon of structural variation in the human genome sequence, describing the novel genomics technologies that are revolutionizing the way structural variation is studied and giving examples of genomic structural variations that affect child development. 相似文献
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植物功能基因组学研究 总被引：5，自引：0，他引：5
基因组研究已进入了以功能基因组研究为特征的后基因组时代.本文介绍了基因组及基因组学的概念及功能基因组学的研究方法,并综述了植物功能基因组学的研究进展. 相似文献
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介绍了用于晶体衍射研究和NMR研究的蛋白质自动化生产的步骤,这些步骤包括建立克隆和表达载体,蛋白质的表达,蛋白质纯化,蛋白质质量控制和NMR或晶体衍射方法的选择. 相似文献
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本论文通过核磁共振实验技术,对吐昔烯盘状液晶化合物的结构特征进行了详细的研究.通一维的1HNMR、13CNMR、DEPT,二维核磁共振gHMBC、gHSQC等实验技术对化合物的碳氢进行了归属;为该化合物结构的解析提供了有利的依据. 相似文献
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Shuster M 《CBE life sciences education》2011,10(2):216-221
In recognition of the entry into the era of personalized medicine, a new set of genetics and genomics competencies for nurses was introduced in 2006. Since then, there have been a number of reports about the critical importance of these competencies for nursing practices and about the challenges of addressing these competencies in the preservice (basic science) nursing curriculum. At least one suggestion has been made to infuse genetics and genomics throughout the basic science curriculum for prenursing students. Based on this call and a review of the competencies, this study sought to assess the impact of incorporation of genetics and genomics content into a prenursing microbiology course. Broadly, two areas that address the competencies were incorporated into the course: 1) the biological basis and implications of genetic diversity and 2) the technological aspects of assessing genetic diversity in bacteria and viruses. These areas address how genetics and genomics contribute to healthcare, including diagnostics and selection of treatment. Analysis of learning gains suggests that genetics and genomics content can be learned as effectively as microbiology content in this setting. Future studies are needed to explore the most effective ways to introduce genetics and genomics technology into the prenursing curriculum. 相似文献
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A series of photochromic diarylethene derivatives containing different fluorophores was synthesized by the Sonogashira coupling reaction and characterized by ^1H nuclear magnetic resonance （NMR）, ^13C NMR and mass spectroscopy. Photochromic conversion of Cz-MS was observed and its structural change was confirmed by proton NMR, Switchable absorption and emission phenomena were observed for these molecules. These phenomena were attributed to the reversible photochromic fluorescence resonance energy transfer （pcFRET） process. The design of this novel photochromic system of Py-4MS enables information to be processed in a non-destructive manner. This overcomes the problem of the destructive nature of tracking photochromism using ultraviolet （UV）-vis spectroscopy. 相似文献
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水稻T-DNA插入突变技术研究 总被引：1，自引：0，他引：1
随着水稻基因组全序列的测定完成,功能基因组学已成为重点研究内容.农杆菌介导的T-DNA标签法是近年发展起来的一种有效的分子生物学技术.它具有程序简便,转化效率高,大规模转化等特点.阐述了T-DNA标签法及其改进后的激活标签技术和增强子技术的特点,并介绍了T-DNA插入突变在水稻突变体库的建立中所取得的研究进展,从而表明T-DNA插入突变技术是水稻功能基因组研究的一个有效途径. 相似文献
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Campbell AM Ledbetter ML Hoopes LL Eckdahl TT Heyer LJ Rosenwald A Fowlks E Tonidandel S Bucholtz B Gottfried G 《CBE life sciences education》2007,6(2):109-118
The Genome Consortium for Active Teaching (GCAT) facilitates the use of modern genomics methods in undergraduate education. Initially focused on microarray technology, but with an eye toward diversification, GCAT is a community working to improve the education of tomorrow's life science professionals. GCAT participants have access to affordable microarrays, microarray scanners, free software for data analysis, and faculty workshops. Microarrays provided by GCAT have been used by 141 faculty on 134 campuses, including 21 faculty that serve large numbers of underrepresented minority students. An estimated 9480 undergraduates a year will have access to microarrays by 2009 as a direct result of GCAT faculty workshops. Gains for students include significantly improved comprehension of topics in functional genomics and increased interest in research. Faculty reported improved access to new technology and gains in understanding thanks to their involvement with GCAT. GCAT's network of supportive colleagues encourages faculty to explore genomics through student research and to learn a new and complex method with their undergraduates. GCAT is meeting important goals of BIO2010 by making research methods accessible to undergraduates, training faculty in genomics and bioinformatics, integrating mathematics into the biology curriculum, and increasing participation by underrepresented minority students. 相似文献