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1.
A known species, Physarum melleum, was found fruiting on living leaves of Dendrobium candidum, which was collected in China in 2004. Its morphological characters were revealed by light microscopy (LM), environmental scanning electron microscopy (ESEM) and scanning electron microscopy (SEM). Character variations were distinguished by its olive-yellow peridium and its always thinner capillitium containing globulose granular material between the large calcareous nodes. The calcium carbonate granules, deposited on stalks, peridium and hypothallus as well as within stalks, were globose and smooth.  相似文献   

2.
In this paper, the Tφ-convex functions were introduced as a generalizations of convex functions. Then the characteristics of the Tφ-convex functions were discussed. Furthermore, some new inequalities for the Tφ-convex functions were derived.  相似文献   

3.
《宝剑记》主要取材于我国古典小说《水浒传》中"林冲落草"这段故事。相较来看,《宝剑记》继承了《水浒传》的很多方面,包括主要人物设置,故事情节内容,"忠奸"主题思想。除此,《宝剑记》在人物形象、情节脉络、中心矛盾、起因结局等方面又有所创新。《宝剑记》对《水浒传》的改编凸显出明中叶反封建专制统治的社会思潮和人的自我意识的觉醒,也是作者李开先个人心灵世界的直接映现,具有明显的进步性与现实意义。  相似文献   

4.
Fusarium head blight (FHB) caused byFusarium graminearum is a devastating disease that results in extensive yield losses to wheat and barley. A green fluorescent protein (GFP) expressing plasmid pRP22-GFP was constructed for monitoring the colonization of two biocontrol agents,Brevibacillus brevis ZJY-116, on the spikes of barley and their effect on suppression of FHB. Survival and colonization of theBrevibacillus brevis ZJY-1 andBacillus subtilis ZJY-116 strains on spikes of barley were observed by tracking the bacterial transformants with GFP expression. Our field study revealed that plasmid pRP22-GFP was stably maintained in the bacterial strains without selective pressure. The retrieved GFP-tagged strains showed that the bacterial population fluctuation accorded with that of the rain events. Furthermore, both biocontrol strains gave significant protection against FHB on spikes of barley in fields. The greater suppression of barley FHB disease was resulted from the treatment of barley spikes with biocontrol agents before inoculation withF. graminearum. Project supported by the National Natural Science Foundation of China (No. 30230250) and Science and Technology Committee of Zhejiang Province (No. 2003C22029), China  相似文献   

5.
Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)  相似文献   

6.
《我的马克思主义观》是李大钊成为马克思主义者的标志,是五四新文化运动的重要转折点,也是马克思主义在中国开始广泛传播的重要标志。有关论著对它的研究和提及很多,《李大钊全集》的编注者对其注解也较多。进一步研究起来,该文中需要探究的问题仍有不少。总起来看,李大钊的《我的马克思主义观》以日本社会主义学者河上肇的《马克思的社会主义理论体系》为重要蓝本,其关于马克思主义三个组成部分与一条“金线”的核心论断也出于河上肇此文;写作和定稿时间,应为1919年7月底到8月上旬,而不可能在1919年5月之前。  相似文献   

7.
Insecticidal effects of different doses of the dust and methanol extracts of Garcinia kolae on Collosobruchus maculatus and Sitophilus zeamais were tested. The dust had no significant effect on the two insects; none of them died even at 3 d after treatment. The methanol extracts, however, had rapid lethal effects on both C. maculatus and S. zeamais. The mortality of C. maculatus by the lowest concentration of methanol extracts ranged from 95%∼100% whereas in S. zeamais, the mortality ranged from 87.5%∼100% and 70%∼100% in concentrations of 1 g extract+3 ml methanol and 1 g extract+5 ml methanol, respectively, from 24 to 48 h. The least concentration of 1 g extract+15 ml methanol had no significant lethal effect on Sitophilus zeamais.   相似文献   

8.
The activities of enzymes responsible for lignification in pepper, pre-inoculation with arbuscular mycorrhizal (AM) fungus ofGlomus intraradices and/or infection with pathogenic strain ofPhytophthora capsici, and the biological control effect ofG. intraradices on Phytophthora blight in pepper were investigated. The experiment was carried out with four treatments: (1) plants pre-inoculated withG. intraradices (Gi), (2) plants pre-inoculated withG. intraradices and then infected withP. capsici (Gi+Pc). (3) plants infected withP. capsici (Pc), and (4) plants without any of the two microorganisms (C). Mycorrhizal colonization rate was reduced by about 10% in pathogen challenged plants. Root mortality caused by infection ofP. capsici was completely eliminated by pre-inoculation with antagonisticG. intraradices. On the ninth day after pathogen infection, Peroxidase (POD) activity increased by 116.9% in Pc-treated roots but by only 21.2% in Gi+Pc-treated roots, compared with the control, respectively. Polyphenol oxidase (PPO) and Phenylalanine ammonia-lyase (PAL) activities gradually increased during the first 3 d and dramatically decreased in Pc-treated roots but slightly decreased in Gi+Pc-treated roots, respectively. On the ninth day after pathogen infection, PPO and PAL decreased by 62.8% and 73.9% in Pc-treated roots but by only 19.8% and 19.5% in Gi+Pc-treated roots, compared with the control, respectively. Three major POD isozymes (45000, 53000 and 114000) were present in Pc-treated roots, while two major bands (53000 and 114000) and one minor band (45000) were present in spectra of Gi+Pc-treated roots, the 45000 POD isozyme was significantly suppressed byG. intraradices, suggesting that the 45000 POD isozyme was induced by the pathogen infection but not induced by the antagonisticG. intraradices. A 60000 PPO isozyme was induced in Pc-treated roots but not induced in Gi+Pc-treated roots. All these results showed the inoculation of antagonisticG. intraradices alleviates root mortality, activates changes of lignification-related enzymes and induces some of the isozymes in pepper plants infected byP. capsici. The results suggested thatG. intraradices is a potentially effective protection agent againstP. capsici. Project supported by Korea Science and Engineering Foundation (KOSEF) through the Agricultural Plants Stress Research Center (APSRC) at Chonnam National University, Korea  相似文献   

9.
Aquilaria agallocha can produce fragrant agarwood used for incense, traditional medicine and other products. An efficient plant regeneration system was established via organogenesis from shoots developed from seedlings ofAquilaria agallocha. Shoots generated many buds on MS medium supplemented with 1.3 μmol/L BA (6-benzylaminopurine) in the first 7 weeks, and the buds elongated on MS medium with 1.3 μmol/L BA+0.5 μmol/L NAA (naphthaleneacetic acid) in another 7 weeks, 2.3 shoots 2 cm in length per explant were obtained within 14 weeks. Plantlets were rooted on 1/2 MS medium after being immersed in 5 μmol/L NAA for 48 h, 96.7% of the roots grew up two weeks later. All plantlets that survived acclimatization grew well in the pots. Project supported by the National Natural Science Foundation of China (No. 30070066) and the Science and Technology Project of Guangzhou City (No. 2003J1-C0241), China  相似文献   

10.
《沧浪诗话》今传"通行本"与"玉屑本"两种版本,其中《诗辩》的文本顺序存在较大差异。文章在综述已有研究成果的基础上,进一步就《诗辩》的文本顺序问题提出四点补论:其一,《沧浪诗话》原非"诗话体"著作,《诗辩》当为"辩"体论说文,而《诗人玉屑》作为诗话著作摘录《诗辩》,并不承担保存其原貌的责任;其二,若依"玉屑本",则《诗辩》开篇即蹈袭北宋《潜溪诗眼》旧话;其三,从两个"识"字可以看出被"玉屑本"腰斩为两段的文字原本存在紧密的逻辑承接关系,且"通行本"在行文脉络上更加明晰;其四,论法论品相关文字或为"通行本"编校者据"玉屑本"补入,是以此两段文字依"玉屑本"当更为合理。  相似文献   

11.
The morphology and ultrastructure of Bullacta exarata spermatozoa observed by light and transmission electron microscopy are presented in this paper. The spermatozoon is composed of head with a simple acrosomal complex and an elongated nucleus, and tail with a midpiece, principal piece and an end piece. The midpiece consists of a mitochondrial ring, and the principal piece is composed of axoneme and lateral fin. The structure of B. exarata spermatozoa differs significantly from that of other gastropods, especially in the lateral fin and the principal piece, which was described scarcely before. A comparison is made between B. exarata and other gastropods, and its significance on reproductive evolution and physio-ecological adaptation is preliminarily discussed. Project supported by the Committee of Natural Science of Zhejiang Province (No. 398081) and Wenzhou Normal College (No. 2001Y15), China  相似文献   

12.
A commercial AA2618 alloy was treated through melt spinning at rotating speeds of 20 and 40 m·s−1. The as-melt spun ribbons were characterized by a combination of optical microscopy (OPM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray diffraction (XRD). The microstructural evolution of the ribbons in a continuous heating process was investigated, and the microhardness was also measured under different conditions. It was found that Al x FeNi is prone to precipitate in AA2618 alloy due to the minimal solubility of iron and nickel. Fine Al x FeNi particles appeared along the grain boundaries at the chilling sides of as-melt spun ribbons, and at both the grain boundaries and in the interior of grains at the free sides. On continuous heating Al x FeNi precipitated steadily and uniformly throughout the matrix until melting. The microhardness of as-melt spun ribbons decreased significantly from the chilling surfaces to free surfaces. Precipitation of Al x FeNi lowered the hardness of the alloy. Project supported by Special Research Foundation of High-Education Ph. D. Subjects, Education Ministry of P. R. China.  相似文献   

13.
Studies were conducted under pot conditions to determine the comparative efficacy of carbofuran at 1 mg a.i./kg soil, bavistin at 1 mg a.i./kg soil, neem (Azadirachta indica) seed powder at 50 mg/kg soil, green mould (Trichoderma harzianum) at 50.0 ml/kg soil, rhizobacteria (Pseudomonas fluorescens) at 50.0 ml/kg soil against root-knot nematode,Meloidogyne incognita-wilt fungus,Fusarium oxysporum disease complex on green gram,Vigna radiata cv ML-1108. All the treatments significantly improved the growth of the plants as compared to untreated inoculated plants. Analysis of data showed that carbofuran andA. indica seed powder increased plant growth and yield significantly more in comparison to bavistin andP. fluorescens. Carbofuran was highly effective against nematode, bavistin against fungus,A. indica seed powder against both the pathogens and both the bioagents were moderately effective against both the pathogens.  相似文献   

14.
Effects of dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus inMiichthys miiuy fries were investigated. Nine hundredMiichthys miiuy fries were divided into 3 groups, each with triplicates. The basal diet and the basal diet supplemented with carnitine groups were considered as the negative and positive controls respectively. Results showed that the nitrogen concentration in excreted feces decreased significantly in fries fed the diet supplementation with 1000×10−6 fructooligosaccharides and 200×10−6 carnitine (P<0.05). The ammonic-nitrogen concentration decreased significantly in the carnitine group only (P<0.05), indicating the decreasing tendency caused by the supplementation with fructooligosaccharides. Supplementation with both did not have significant effects on the concentration of phosphorus in feces ofMiichthys miiuy fries. Project supported by Public Bidding Item in Key Research of Zhejiang Province (No. 02110281-2) and Ningbo Tackle Key Problem for Agricultural Development (No. 2004C-100030), China  相似文献   

15.
The study was conducted to investigate fasting effects on flesh composition and antioxidant defenses of market-size Sparus macrocephalus. Two hundred fish (main initial weight 580 g) were divided into two groups (control and fasted) and reared in 6 cages. After two weeks of adaptation, group I fasted for 28 d; group II was fed normally as a control. In 3, 7, 14, 21 and 28 d, 6 fish per group were sampled for proximate flesh composition, liver antioxidant enzyme activities and malondialdehyde flesh content analyses. In fasted fish, the reduction of lipid content in muscle occurred after day 3, and, compared to controls, the content of protein decreased from day 14, the activities of liver antioxidative enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPX) increased from day 3, and flesh malondialdehyde levels increased from day 21. Flesh fat reduction shows that fasting may be used as a technique to reduce flesh lipid content in Sparus macrocephalus. However, considering flesh protein loss and the subsequent oxidative stress, the fasting technique should be used with precautions. Project (No. 2006C12098) supported by the Science and Technology Department of Zhejiang Province, China  相似文献   

16.
Fasciclin family proteins have been identified as cell adhesion molecules in various organisms. In this study, a novel Magnaporthe oryzae fasciclin-like protein encoding gene, named MoFLP1, was isolated from a subtractive suppressive cDNA library and functionally analyzed. Sequence analysis showed that the MoFLP1 gene contains an open reading frame (ORF) of 1050 nucleotides encoding 349 amino acids with a calculated molecular weight of 35.85 kDa and a pI of 7.76. The deduced MoFLP1 protein contains a 17-amino acid secretion signal sequence and an 18-amino acid sequence with the characteristics of a glycosylphosphotidylinositol (GPI) anchor additional signal at its N- and C-terminuses, respectively. Potential N-glycosylation sites and domains involving cell adhesion were also identified in MoFLP1. Sequence analysis and subcellular localization by the expression of MoFLP1-GFP fusion construct in M. oryzae indicated that the MoFLP1 protein is probably localized on the vacuole membrane. Two MoFLP1 null mutants generated by targeted gene disruption exhibited marked reduction of conidiation, conidial adhesion, appressorium turgor, and pathogenicity. Our results indicate that fasciclin proteins play important roles in fungal development and pathogenicity in M. oryzae. Project supported by the National Natural Science Foundation of China (No. 30870101) and the Public Welfare Profession (Agriculture) Research Project (No. 200803008), China  相似文献   

17.
1H-NMR and13C-NMR assignments of 12-oleanene-3,11-dione (compound 1) were completely described for the first time through conventional 1D NMR and 2D shift-correlated NMR experiments using1H-1HCOSY, HMQC, HMBC techniques. Based on its NMR data, the assignments of 28-hydroxyolean-12-ene-3,11-dione (compound 2) were partially revised. Project (Nos. 20375036 and 20472073) supported by the National Natural Science Foundation of China  相似文献   

18.
Surface morphologies of Zr52.5 Al10 Ni10 Cu15 Be12.5 bulk metallic glass after being rolled at both a temperature around T9 and near （ Tx - 50） K were investigated with a scanning electron microscopy. Macroscopic and microscopic observation results show that squamae, cracks, steps and wedges exist on the surface when the samples were rolled at temperatures around Ty. However, a smooth and fiat surface appears when the samples were rolled at temperatures near （ Tx - 50） K. These results indicate that the mode of deformation in the supercooled liquid region is a partially homogeneous flow at a temperature around T9, and a fully homogeneous one at temperatures near （ Tx - 50） K. According to the results, it is more feasible to roll the amorphous alloys at temperatures near （ Tx - 50） K to obtain parts with smooth and fiat surface.  相似文献   

19.
恩斯特·布洛赫在《希望的原理》一书中,对马克思主义的纲领性文件《关于费尔巴哈的提纲》展开了全新解读。在他看来,马克思主义并非简单的政治经济学批判,而是一种以劳动、异化和实践概念为核心的具体的人本主义思想。布洛赫的解读一方面恢复了"人"的主体地位,克服了正统马克思主义的机械经济决定论倾向,另一方面由于过度关注伦理价值,这种解读也背离了马克思主义立足现实追求人类解放的基本精神,其思想局限也是较为明显的。  相似文献   

20.
The endangered medicinal herb,Changium smyrnioides can only be found in deciduous forest gaps within the middle to northern subtropical broad-leaved evergreen forest zone of China. The considerable plasticity of its shoot and root structure helps it to capture light more effectively in winter and early spring, and to adapt to the soil moisture conditions in its narrow habitat. Another medicinal plant,Anthriscus sylvestris, is of similar economic importance but commonly distributed widely. In contrast toC. smyrnioides, it has low structural plasticity. It is also specialized to adapt to the moist and sunny environment, where habitat, such as the banks of creeks and rivers, is abundant. Project supported by the National Basic Research Program (973) of China (No. G2000046805) and the National Natural Science Foundation of China (No. 39970058)  相似文献   

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