共查询到17条相似文献，搜索用时 875 毫秒
1.
《常熟理工学院学报》2017,(2)
以黑皮鸡枞菌深层液体发酵菌丝体生物量为指标,首先通过单因素试验确定了最佳的培养温度、摇床转速、pH值、发酵周期,然后经过响应面试验的优化,得出最佳发酵条件:培养温度为26℃,摇床转速为175 r/min,pH值为7.6,发酵周期为25 d.通过验证培养后,黑皮鸡枞菌丝体干重达6.91 mg/mL. 相似文献
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通过单因子实验,筛选得到了适合大豆疫霉拮抗细菌B048液体发酵的最佳碳源为玉米粉,最佳氮源为豆粕,最佳无机盐离子为氯化钠。通过正交实验确定了该培养基的最佳配比为：玉米粉5g／L、豆粕15g／L、氯化钠10g／L。在确定适合该菌株的培养基组分基础上,对其培养条件进行优化,结果表明：初始pH值为6．0,培养时间为48h,转速为180r／min时为该菌株的最佳培养条件。 相似文献
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通过改变传统的冬虫夏草菌种制备的方法,利用其菌索来制备,并对其菌丝体的液体培养基的配方进行优化.结果表明,冬虫夏草生长最适宜液体培养基的配方为葡萄糖7.5%、蔗糖7.5%、蛋白胨0.12%、酵母粉0.375%、KH2PO40.15%、MgSO4·7H2O0.075%、VB10.015%、pH自然,20℃条件下培养菌丝体192 h,菌丝的量达到4.242g/L,并且在以后的二、三级菌种培养过程中,菌丝的性状表现优良,生长速度较快. 相似文献
4.
研究了羊肚菌深层发酵培养基中玉米粉和黄豆粉配比和发酵条件,确定了羊肚菌深层发酵最佳配比的培养基以及最佳的发酵温度、起始pH和摇床转速.通过单因素确定培养基中玉米粉和黄豆粉的最佳配比,在单因素试验的基础上采用三因素三水平的响应面分析法确定羊肚菌液体发酵的培养条件.结果以羊肚菌菌丝体生物量和胞外多糖浓度为响应面和等高线,在分析各个因素的显著性和交互作用后,得出羊肚菌液体发酵的最佳温度25.33℃、起始pH 6.13、摇床转速154.33r/min.在最佳发酵条件下,发酵144h,羊肚菌的菌丝体生物量和胞外多糖浓度达到最高,分别为7.78g/L和130.97mg/L. 相似文献
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通过正交交互设计试验优化灰树花的液体培养基配方,确定玉米粉和葡萄糖有交互作用,其比例为3∶2时为最优搭配。获得灰树花液体培养的最优配方为黄豆粉2%,玉米粉3%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.2%,豆油0.05%。将灰树花在50L发酵罐中进行深层培养,转速140r/min和通气量为300L/min,菌丝体产量为14.69g/L。 相似文献
6.
南韩灵芝母种培养基的筛选研究 总被引：1，自引：0，他引：1
通过对南韩灵芝菌株在不同碳源、氮源及不同配方的母种培养基的生长实验,筛选出适合其生长的碳源、氮源和最佳培养基配方．试验结果表明：南韩灵芝菌丝体在麸皮、酵母膏,蛋白胨3种氮源中,以麸皮为最适氮源;在葡萄糖、蔗糖和玉米粉3种碳源中,以玉米粉为最适碳源．在5种不同的培养基试验中,加富PDA培养基是最适合南韩灵芝生长的培养基配方． 相似文献
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实验采用单因素试验优化纳豆菌液体发酵条件。通过蛋白凝块溶解时间法测定纳豆激酶活力,筛选出最佳培养条件。液态发酵选用甘油、乳糖以及木糖与葡萄糖的混合糖代替基础培养基中的麦芽糖;用酵母膏、干酪素、胰蛋白胨和黄豆汁代替基础培养基中的麸皮进行发酵产酶试验,筛选出最佳碳氮源,并在此基础上变换不同的碳、氮源浓度,筛选出最佳的碳、氮比。试验结果表明：液态发酵最佳条件为,甘油10％,酵母膏2％,明胶0．5％,NaCl 0．5％,KH2PO4 0．1％,K2HPO4 0．4％,MgSO4 0．05％,初始pH7．0。在此条件下培养,测得的纳豆激酶活力相当于尿激酶1081．22IU／mL。与此前报道结果800．50IU／mL相比有了明显的提高。 相似文献
9.
以平菇为材料,分析了不同浓度的Fe^2＋在其菌丝体和子实体中的富集及其生长的影响．试验表明：在液体培养基中添加150mg／L的Fe^2＋,平菇菌丝体中铁的含量最高为1．4mg／100g干重;在液体培养基中添加100mg／L和150mg／L的Fe^2＋,平菇菌丝体吸收铁的效率最高为2．0％;固体培养基Fe^2＋浓度为125mg／L时能明显促进菌丝体的生长;子实体中添加5gFeSO4．7H2O时能明显提高其产量,子实体中添加2．5gFeSO4．7H2O时吸收Fe^2＋的效率最高,为1．19％． 相似文献
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通过对突变株Bacillus subtilis spp.产β-葡聚糖酶的研究,得出其最佳培养基配方为（/100mL）：最佳培养基配方为（g/100ml）：大麦粉3.0,玉米粉2.0,豆粕粉4.0,MgSO40.03,CaCO30.05;在最佳培养基条件下进行摇瓶培养（35mL培养基/250mL三角瓶）,发酵液酶活力达56.53U/ml,是初始产酶条件的1.8倍。 相似文献
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研究了大豆根瘤菌AWCS13-4菌株在YMA、TY、PA、BSE 4种培养基中的生长情况,并通过单因素和正交试验确定最佳培养条件。结果表明,AWCS13-4菌株在YMA培养基中生长较好,蔗糖为最佳碳源,酵母粉为最佳氮源,最佳p H为7.0,最佳接种量为3%,最佳培养温度为30℃。在单因素的基础上,采用正交试验,对4个因素进行优化,其最佳培养组合为:每升培养基中添加蔗糖10 g,酵母粉3 g,Mg SO4·7H_2O 0.2 g,K2HPO40.5 g,NaCl 0.1 g,CaCl_2·5H_2O 0.05 g,Rh微量元素液4 m L;p H为7.0,接种量为3%,培养温度为30℃。 相似文献
12.
双孢蘑菇液体菌种培养基的优化 总被引：1，自引：0，他引：1
采用正交试验优化双孢蘑菇的液体培养基配方,发现黄豆粉、葡萄糖、硫酸镁、以及玉米粉和葡萄糖的交互作用对双孢蘑菇的液体培养有显著影响,磷酸二氢钾、麦麸汁对实验结果无显著影响。双孢蘑菇最佳液体培养基配方为:黄豆粉2%,玉米粉2%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.20%,麦麸0.20%。把双孢蘑菇液体菌种接入小麦栽培种中,菌丝表现出生长快的优势,生长速度较为一致,满瓶时间短,证明利用液体菌种制备栽培种比固体菌种具有更大的优势。 相似文献
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以饲料为原料筛选出一株既产乳酸又产芽孢的益生菌,经过分子生物学鉴定为凝结芽孢杆菌(Bacillus coagulans),命名为T-8。从培养基碳源、氮源、有机物浸出液、培养基pH、培养温度、培养时间等方面对菌株T-8展开发酵特性研究,结果表明,最优培养基配方为:玉米粉6 g/L、豆粕粉15 g/L、小麦麸皮浸出液60%、酵母粉5 g/L、蛋白胨10 g/L、氯化钠10 g/L,pH 5.0。以5%的接种量将种子液转接至最优培养基,在47℃的摇床中以200 r/min的转速振荡培养28 h,菌株T-8的细胞密度达到4.8×109 CFU/mL,芽孢率达到95.2%。本试验可为益生菌凝结芽孢杆菌应用于饲用微生物菌剂的工业化生产提供参考。 相似文献
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Xu YX Li YL Xu SC Liu Y Wang X Tang JW 《Journal of Zhejiang University. Science. B》2008,9(7):558-566
Objective: To study the optimal medium composition for xylanase production by Aspergillus niger XY-1 in solid-state fermentation (SSF). Methods: Statistical methodology including the Plackett-Burman design (PBD) and the central composite design (CCD) was employed to investigate the individual crucial component of the medium that significantly affected the enzyme yield. Results: Firstly, NaNO3, yeast extract, urea, Na2CO3, MgSO4, peptone and (NH4)2SO4 were screened as the significant factors positively affecting the xylanase production by PBD. Secondly, by valuating the nitrogen sources effect, urea was proved to be the most effective and economic nitrogen source for xylanase production and used for further optimization.Finally, the CCD and response surface methodology (RSM) were applied to determine the optimal concentration of each sig-nificant variable, which included urea, Na2CO3 and MgSO4. Subsequently a second-order polynomial was determined by mul-tiple regression analysis. The optimum values of the critical components for maximum xylanase production were obtained as follows: x1 (urea)=0.163 (41.63 g/L), x2 (Na2CO3)=-1.68 (2.64 g/L), x3 (MGSO4)=1.338 (10.68 g/L) and the predicted xylanase value was 14374.6 U/g dry substrate. Using the optimized condition, xylanase production by Aspergillus niger XY-1 after 48 h fermentation reached 14637 U/g dry substrate with wheat bran in the shake flask. Conclusion: By using PBD and CCD, we obtained the optimal composition for xylanase production by Aspergillus niger XY-1 in SSF, and the results of no additional expensive medium and shortened fermentation time for higher xylanase production show the potential for industrial utilization. 相似文献
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A newly isolated strain EL31410, producing elastase (E. C3. 4. 4. 7) with high elastolytic activity was identified asBacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen
source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production.
A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite
experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran
and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but
soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4% (w/v) wheat bran and 9.4%
(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase
production in the optimized medium composition was also described.
Project (No. 300024) supported by the Zhejiang Provincial Natural Science Foundation of China 相似文献
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A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production. A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4%(w/v) wheat bran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase production in the optimized medium composition was also described. 相似文献
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采用溶胶凝胶法制备了稀土铈掺杂的纳米氧化锌.通过X射线衍射(XRD)、紫外-可见光谱(UV-Vis)和红外光谱(FTIR)分析等对样品的微观结构和性能进行了表征.结果表明:Ce4+成功掺入ZnO中,结晶质量良好,粒径约为10.7 nm;UV-Vis曲线有红移现象.产品的光催化活性以亚甲基蓝(MB)为评价模型,对掺杂比例、表面活性剂种类、煅烧温度、煅烧时间等条件进行了实验研究,得出了制备纳米氧化锌的最优条件.即最佳的掺杂比例为n(Ce):n(Zn)=0.05、表面活性剂为十二烷基磺酸钠、煅烧温度为550℃、煅烧时间为3.0 h.同时考察了染料溶液起始pH值以及催化剂的用量,光照时间等对催化效率的影响.结果表明:当MB溶液浓度为10 mg/L,溶液pH值为7~8、催化剂的用量为5 g/L、光照时间2 h后降解率可达85%以上.在上述条件下,催化剂对实际染料脱色率达87.67%,CODCr去除率为63.5%. 相似文献