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1.
以怀山药为材料,研究了不同外植体(叶片、茎段、零余子)对愈伤组织形成和植株再生的影响。结果表明:叶片、茎段、零余子在KT2(mg/L,单位下同)+NAA2激素组合培养基上均能产生愈伤组织,且出愈率最高,分别为47.7%、79.5%和100%。零余子和茎段均能培养一次成苗。零余子KT2+NAA2激素组合培养基上成苗率最高,达到50%;而茎段成苗则以6-BA2+NAA0.02效果最好,其成苗率为25%。叶片不能成苗。  相似文献   

2.
野葛愈伤组织的诱导和异黄酮的产生   总被引:1,自引:0,他引:1  
野葛叶片、茎段在适宜激素诱导下均能形成愈伤组织,但不同的激素组合其愈伤组织诱导率不同。4因子3水平的正交实验表明叶片愈伤组织诱导的最适培养基为MS 2,4-D1.0 6-BA0.5 NAA0.5 IBA0.5mg/l,茎段愈伤组织诱导的最适培养基为MS 6-BA1.5 NAA1.0 IBA0.5mg/l。茎段的愈伤组织诱导优于叶片,光照有利于愈伤组织的诱导。茎、叶形成的愈伤组织产生异黄酮的能力相同。  相似文献   

3.
不同植物生长调节剂对益母草愈伤组织诱导的影响   总被引:3,自引:0,他引:3  
以益母草茎段、叶片两种外植体诱导产生愈伤组织.在MS培养基上,试验不同种类、不同浓度的植物生长调节剂及其组合对益母草茎段和叶片愈伤组织诱导的影响.结果表明:茎段愈伤组织诱导的较适培养基是MS附加6-BA3mg/L+NAA1mg/L,诱导率为65%;叶片愈伤组织诱导的较适培养基是MS附加6-BA2mg/L+NAA1mg/L,诱导率为80%.对益母草茎段和叶片外植体愈伤组织进行诱导,可为进一步研究益母草的组织培养和植株再生提供实验依据.  相似文献   

4.
仙客来叶片的组织培养体细胞胚诱导研究   总被引:4,自引:0,他引:4  
利用引种仙客来的叶片为外植体进行组织培养愈伤组织和体细胞胚诱导 ,在MS +BA 2 (mg L ,下同 ) +KT 0 2 +2 ,4_D 0 5或MS +BA 2 +KT 0 2 +NAA 0 5的培养基上 ,均能诱导愈伤组织、胚状体和丛生芽 ,其诱导率为 10 0 % .  相似文献   

5.
珙桐愈伤组织诱导和继代培养中的褐化研究   总被引:3,自引:0,他引:3  
以珙桐的叶片作外植体,对影响诱导愈伤组织的条件和基本培养基进行筛选,并对愈伤组织继代培养中的褐变问题进行研究。结果表明:WPM是珙桐叶片初代培养的最佳基本培养基;2,4-D与KT组合比NAA与6-BA组合对珙桐叶片愈伤组织诱导有更显著的影响,最佳诱导培养基组合为WPM+2,4-D1.0mg/L+KT2.0mg/L,继代培养中添加1.0—1.5mg/L抗坏血酸(VC)能有效抑制愈伤组织褐化。  相似文献   

6.
景天三七的组织培养——愈伤组织诱导   总被引:1,自引:0,他引:1  
以景天三七的茎段和叶片作为外植体,采用MS培养基为基本培养基,附加不同的植物激素组合进行实验,诱导其产生愈伤组织.结果表明:适合于离体茎段和叶片愈伤组织诱导的培养基是MS+6-BA1.0mg/L+2,4-D1.0 mg/L,诱导率分别为66.67%和76.19%.  相似文献   

7.
采用红掌(Anthurium andreanum Dakota)幼叶叶片中部(带主叶脉叶片),叶片基部(叶柄与叶片连接处),叶尖部位作为外植体,接种在1/4 MS附加不同浓度的6-BA+2,4-D+KT的培养基上.结果表明,红掌(Anthurium andreanum)愈伤组织的诱导率不仅与叶片不同部位有关,而且与添加外源激素的浓度及配比有关.经过60 d愈伤组织诱导培养,叶片基部作为外植体诱导愈伤组织效果最好,愈伤组织诱导率最高的培养基为1/4 MS+6-BA0.9 mg/L+2,4-D 0.9 mg/L+KT0.5 mg/L.  相似文献   

8.
红掌(Anthurium andreanum Dakota)诱导愈伤组织研究幼叶   总被引:1,自引:0,他引:1  
采用红掌(Anthurium andreanum Dakota)幼叶叶片中部(带主叶脉叶片),叶片基部(叶柄与叶片连接处),叶尖部位作为外植体,接种在1/4MS附加不同浓度的6-BA+2,4-D+KT的培养基上.结果表明,红掌(Anthurium andreanum)愈伤组织的诱导率不仅与叶片不同部位有关,而且与添加外源激素的浓度及配比有关.经过60d愈伤组织诱导培养,叶片基部作为外植体诱导愈伤组织效果最好,愈伤组织诱导率最高的培养基为1/4 MS+6-BA 0.9mg/L+2,4-D 0.9mg/L+KT 0.5mg/L.  相似文献   

9.
以绞股蓝茎尖、茎段、叶片为外植体,研究了不同种类、不同浓度配比的外源激素对绞股蓝愈伤组织诱导的影响。结果表明:茎尖在培养基MS+6- BA 1.0 mg·L-1+NAA 0.02 mg·L-1中的诱导效果最好,其诱导率为75.0%;茎段在培养基MS+6- BA 2.0 mg·L-1+NAA 0.2 mg·L-1中的诱导效果最好,其诱导率为81.8%;叶片在培养基MS+6- BA 1.0 mg·L-1+NAA 0.15 mg·L-1中的诱导效果较好,其诱导率为69.2%。  相似文献   

10.
朱缨花愈伤组织的诱导   总被引:1,自引:0,他引:1  
研究了不同外植体和不同生长调节剂对朱缨花愈伤组织诱导的影响.结果表明:不同外植体的愈伤组织诱导能力为茎段〉带节茎段〉叶柄〉叶片,茎段和带节茎段是愈伤组织诱导的最佳外植体;诱导朱缨花茎段愈伤组织的最佳生长调节剂组合为MS+NAA1mg/L和MS+BA0.5mg/L+NAA1mg/L;诱导朱缨花带节茎段愈伤组织的最佳生长调节剂组合为MS+BA2mg/L+NAA1.5mg/L.  相似文献   

11.
The communication of people partially is the communication of cultures. Culture has a direct effect on international commercial activities in all aspects. Different conceptions about time, space, equality, law and the like, lead people to deal with things in different ways. So to know cultures of the counterpart is to facil-itate our enterprises so as to have a smooth and successful communication in commercial activity.  相似文献   

12.
汉字倒说     
汉字的六书,《说文》对“转注”一类,语焉不详。后世学者提及转注,也仅限于许慎所举出的例字。《汉字例说》一文,作者从转注的角度综合考虑,对部分现代常用字作了分析,跟传统的解释有所不同。希望能抛砖引玉,互相切磋,以推进学术研究。  相似文献   

13.
风的曲线     
Rosco and I wait for the fishermen to return.I sit at a wooden bench near the store at Mt.Baker Resort and watch the clouds change shape. Rosco has my belt around his neck and an eight foot tow chain hooked to a tree. Dogs must be on a leash. Ducks and rabbits are loose.  相似文献   

14.
Given a graph G,a subgraph C is called a clique of G if C is a complete subgraph of G maximal under inclusion and |C|≥2. A clique-transversal set S of G is a set of vertices of G such that S meets all cliques of G. The clique-transversal number, denoted as TC (G), is the minimum cardinality of a clique-transversal set in G. The clique-graph of G, denoted as K (G), is the graph obtained by taking the cliques of G as vertices, and two vertices are adjacent if and only if the corresponding cliques in G have nonempty intersection. Let F be a class of graphs G such that F={G|K(G) is a tree}. In this paper the graphs in F having independent clique-transversal sets are shown and thus TC (G)/|G|≤1/2 for all G ∈ F.  相似文献   

15.
《海外英语》2007,(5):44-45
It is worthy of noting that, whilst Crookston Castle witnessed the earlier and happier portion of Mary's variegated life,  相似文献   

16.
一、吃和喝吃苹果 eat an apple, 吃药 take medicine,吃糖 have some sweets,吃饭 have one's meals,吃馆子 dine out,吃惊 be surprised/  相似文献   

17.
《海外英语》2007,(5):10-11
Many college freshmen arrive woefully unprepared to do college work, and as disadvantaged populations continue to grow, the share of the American work force that has made it through college is expected to plummet. Many experts blame that educational failure not just on high schools but also on colleges. School & College, a special report by The Chronicle, looks at efforts to fix the system. What reforms would better prepare students for college? What should schools and colleges be doing differently? How should state and federal officials help?  相似文献   

18.
Different nations have different social cultures, and the social culture of every nation is developing with the development of the nation, language is greatly influenced by social culture, which enables language to possess national and historical characteristics. Word is the smallest, independent, meaningful linguistic unit of language. It can well reflect the national and historical characteristics. From synchronic aspect, the social and cultural influence on word meaning is mainly embodied in conceptual and associative meanings of word. The incorrespondence of word meaning is a phenomenon that exists in both conceptual and associative meanings of word among different languages. From diachronic aspect, the development of social culture causes some changes of word meaning to some extent. Therefore, social culture plays a key role in vocabulary learning and mastery of a language.  相似文献   

19.
钱生钱!     
我们绝大部分人都同意一件事情——我们喜欢金钱。但是你对金钱的喜欢之情足够到让你创立一份事业么?.财富通常被人忽视,主要在于人们不能真正了  相似文献   

20.
The problem on the geometrc inequalities involving an n-dimensional simplex and its inscribed simplex is studied. Aninequality is established, which reveals that the difference between the squared circumradius of the n-dimensional simplex andthe squared distance between its circumcenter and barycenter times the squared circumradius of its inscribed simplex is not lessthan the 2(n-1)th power of n times its squared inradius, and is equal to when the simplex is regular and its inscribed siplex is atangent point one. Deduction from this inequality reaches a generalization of n-dimensional Euler inequality indicating that thecircumradius of the simplex is not less than the n-fold inradius. Another inequality is derived to present the relationship betweenthe circumradius of the n-dimensional simplex and the circumradius and inradius of its pedal simplex.  相似文献   

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