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1.
Cell migration is involved in physiological processes such as wound healing, host defense, and cancer metastasis. The movement of various cell types can be directed by chemical gradients (i.e., chemotaxis). In addition to chemotaxis, many cell types can respond to direct current electric fields (dcEF) by migrating to either the cathode or the anode of the field (i.e., electrotaxis). In tissues, physiological chemical gradients and dcEF can potentially co-exist and the two guiding mechanisms may direct cell migration in a coordinated manner. Recently, microfluidic devices that can precisely configure chemical gradients or dcEF have been increasingly developed and used for chemotaxis and electrotaxis studies. However, a microfluidic device that can configure controlled co-existing chemical gradients and dcEF that would allow quantitative cell migration analysis in complex electrochemical guiding environments is not available. In this study, we developed a polydimethylsiloxane-based microfluidic device that can generate better controlled single or co-existing chemical gradients and dcEF. Using this device, we showed chemotactic migration of T cells toward a chemokine CCL19 gradient or electrotactic migration toward the cathode of an applied dcEF. Furthermore, T cells migrated more strongly toward the cathode of a dcEF in the presence of a competing CCL19 gradient, suggesting the higher electrotactic attraction. Taken together, the developed microfluidic device offers a new experimental tool for studying chemical and electrical guidance for cell migration, and our current results with T cells provide interesting new insights of immune cell migration in complex guiding environments. 相似文献
2.
The wound-healing assay is an easy and economical way to quantify cell migration under diverse stimuli. Traditional assays such as scratch assays and barrier assays are widely and commonly used, but neither of them can represent the complicated condition when a wound occurs. It has been suggested that wound-healing is related to electric fields, which were found to regulate wound re-epithelialization. As a wound occurs, the disruption of epithelial barrier short-circuits the trans-epithelial potential and then a lateral endogenous electric field is created. This field has been proved invitro as an important cue for guiding the migration of fibroblasts, macrophages, and keratinocytes, a phenomenon termed electrotaxis or galvanotaxis. In this paper, we report a microfluidic electrical-stimulated wound-healing chip (ESWHC) integrating electric field with a modified barrier assay. This chip was used to study the migration of fibroblasts under different conditions such as serum, electric field, and wound-healing-promoting drugs. We successfully demonstrate the feasibility of ESWHC to effectively and quantitatively study cell migration during wound-healing process, and therefore this chip could be useful in drug discovery and drug safety tests. 相似文献
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4.
The discovery of new drugs is often propelled by the increasing resistance of parasites to existing drugs and the availability of better technology platforms. The area of microfluidics has provided devices for faster screening of compounds, controlled sampling/sorting of whole animals, and automated behavioral pattern recognition. In most microfluidic devices, drug effects on small animals (e.g., Caenorhabditis elegans) are quantified by an end-point, dose response curve representing a single parameter (such as worm velocity or stroke frequency). Here, we present a multi-parameter extraction method to characterize modes of paralysis in C. elegans over an extended time period. A microfluidic device with real-time imaging is used to expose C. elegans to four anthelmintic drugs (i.e., pyrantel, levamisole, tribendimidine, and methyridine). We quantified worm behavior with parameters such as curls per second, types of paralyzation, mode frequency, and number/duration of active/immobilization periods. Each drug was chosen at EC75 where 75% of the worm population is responsive to the drug. At equipotent concentrations, we observed differences in the manner with which worms paralyzed in drug environments. Our study highlights the need for assaying drug effects on small animal models with multiple parameters quantified at regular time points over an extended period to adequately capture the resistance and adaptability in chemical environments. 相似文献
5.
提出了一种改进的适合于ScanSAR成像的多普勒中心估计方法。算法首先在各个子带内求得解卷绕的多普勒基带中心估计;随后利用各子带间脉冲重复频率（PRF）的差异,以最小均方误差准则寻找合适的模糊数组合,从而解决多普勒模糊问题。与以往方法不同之处在于,多普勒模糊的解法基于整个回波数据集,而不依赖于相邻子带重叠区数据,且不需要关于信号或噪声分布的先验知识。算法假设子带间的方位向天线指向变化足够小,以致由此导致的多普勒中心的变化远小于PRF的差异,从而可以近似认为多普勒中心是随距离连续变化的。该算法容易实现,并且能有效保证多普勒中心估计的精度和效率。 相似文献
6.
Yu-Chiu Kao Meng-Hua Hsieh Chung-Chun Liu Huei-Jyuan Pan Wei-Yu Liao Ji-Yen Cheng Po-Ling Kuo Chau-Hwang Lee 《Biomicrofluidics》2014,8(2)
We employed direct-current electric fields (dcEFs) to modulate the chemotaxis of lung cancer cells in a microfluidic cell culture device that incorporates both stable concentration gradients and dcEFs. We found that the chemotaxis induced by a 0.5 μM/mm concentration gradient of epidermal growth factor can be nearly compensated by a 360 mV/mm dcEF. When the effect of chemical stimulation was balanced by the electrical drive, the cells migrated randomly, and the path lengths were largely reduced. We also demonstrated electrically modulated chemotaxis of two types of lung cancer cells with opposite directions of electrotaxis in this device. 相似文献
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设计了一种新型电动机.它是利用暂态电流通电、断电自感与互感形成大小变化的脉冲和一定相位差的多次这类脉冲,产生跳跃式的旋转磁场,使得铝芯转子转动. 相似文献
8.
We examined the performance of three microfluidic devices for stretching DNA. The first device is a microchannel with a contraction, and the remaining two are the modifications to the first. The modified designs were made with the help of computer simulations [C. C. Hsieh and T. H. Lin, Biomicrofluidics 5(4), 044106 (2011) and C. C. Hsieh, T. H. Lin, and C. D. Huang, Biomicrofluidics 6, 044105 (2012)] and they were optimized for operating with electric field. In our experiments, we first used DC electric field to stretch DNA. However, the experimental results were not even in qualitative agreement with our simulations. More detailed investigation revealed that DNA molecules adopt a globular conformation in high DC field and therefore become more difficult to stretch. Owing to the similarity between flow field and electric field, we turned to use flow field to stretch DNA with the same devices. The evolution patterns of DNA conformation in flow field were found qualitatively the same as our prediction based on electric field. We analyzed the maximum values, the evolution and the distributions of DNA extension at different Deborah number in each device. We found that the shear and the hydrodynamic interaction have significant influence on the performance of the devices. 相似文献
9.
In this paper, we report a new method to incorporate 3D scaffold with electrotaxis measurement in the microfluidic device. The electrotactic response of lung cancer cells in the 3D foam scaffolds which resemble the in vivo pulmonary alveoli may give more insight on cellular behaviors in vivo. The 3D scaffold consists of ordered arrays of uniform spherical pores in gelatin. We found that cell morphology in the 3D scaffold was different from that in 2D substrate. Next, we applied a direct current electric field (EF) of 338 mV/mm through the scaffold for the study of cells’ migration within. We measured the migration directedness and speed of different lung cancer cell lines, CL1-0, CL1-5, and A549, and compared with those examined in 2D gelatin-coated and bare substrates. The migration direction is the same for all conditions but there are clear differences in cell morphology, directedness, and migration speed under EF. Our results demonstrate cell migration under EF is different in 2D and 3D environments and possibly due to different cell morphology and/or substrate stiffness. 相似文献
10.
Shunbo Li Ming Li Kristelle Bougot-Robin Wenbin Cao Irene Yeung Yeung Chau Weihua Li Weijia Wen 《Biomicrofluidics》2013,7(2)
Integrating different steps on a chip for cell manipulations and sample preparation is of foremost importance to fully take advantage of microfluidic possibilities, and therefore make tests faster, cheaper and more accurate. We demonstrated particle manipulation in an integrated microfluidic device by applying hydrodynamic, electroosmotic (EO), electrophoretic (EP), and dielectrophoretic (DEP) forces. The process involves generation of fluid flow by pressure difference, particle trapping by DEP force, and particle redirect by EO and EP forces. Both DC and AC signals were applied, taking advantages of DC EP, EO and AC DEP for on-chip particle manipulation. Since different types of particles respond differently to these signals, variations of DC and AC signals are capable to handle complex and highly variable colloidal and biological samples. The proposed technique can operate in a high-throughput manner with thirteen independent channels in radial directions for enrichment and separation in microfluidic chip. We evaluated our approach by collecting Polystyrene particles, yeast cells, and E. coli bacteria, which respond differently to electric field gradient. Live and dead yeast cells were separated successfully, validating the capability of our device to separate highly similar cells. Our results showed that this technique could achieve fast pre-concentration of colloidal particles and cells and separation of cells depending on their vitality. Hydrodynamic, DC electrophoretic and DC electroosmotic forces were used together instead of syringe pump to achieve sufficient fluid flow and particle mobility for particle trapping and sorting. By eliminating bulky mechanical pumps, this new technique has wide applications for in situ detection and analysis. 相似文献
11.
Conventional direct torque control (DTC) suffers from large torque ripple and nonconstant switching frequency, which are caused by the hysteresis band amplitude and the motor speed. Many methods have been proposed to tackle these problems. However, these methods are usually complicated and parameter dependent. A novel DTC method for brushless DC motors based on duty ratio control is proposed to reduce torque ripple and maintain a constant switching frequency. During each switching period, an active voltage vector and a zero voltage vector are applied. A simple and effective method implemented to calculate the duty ratio relies only on the torque error, reducing the parameter dependence. The proposed method has the advantages of conventional DTC and effectively reduces torque ripple, which improves the performance of conventional DTC. Simulation and experimental results are given to confirm the method’s validity. 相似文献
12.
《Journal of The Franklin Institute》2002,339(6-7):499-520
In this paper we are concerned with the problems of (1) tracking or estimating the unknown, time-varying instantaneous frequency (IF) of a chirp signal from a multi-component signal (we assume our multi-component signal to be formed of additive chirp signals, disjoint in the time–frequency domain, and Gaussian noise) and (2) reconstructing a specific chirp signal based on the estimate of its IF found at (1). The algorithm we developed is based on a previously proposed method adapted now for the case of multi-component signals. It combines an adaptive smoothing procedure with a noise resistant Fourier filter to generate an algorithm with an extremely fine frequency resolution. The method is non-parametric, that is, we assume no prior knowledge about the form of the time-varying IF of the chirp or about the chirp itself. We demonstrate how the method works on simulated data and compare its performance to other presently used procedures. 相似文献
13.
The soil dwelling nematode Caenorhabditis elegans (C. elegans) is an excellent model organism for the study of numerous disease including neurodegenerative disease. In this study, a programmable microvalve-based microfluidic array for real-time and long-term monitoring of the neurotoxin-induced responses of the individual C. elegans was developed. The device consisted of a flow layer and a control layer, which were used for worm manipulation. By activating the programmable microvalves in the control layer, mutiple worms could be individually captured and intermittently immobilized in parallel channels. Thus the mobility behavior, together with the corresponding dopaminergic neuron features of the worms in response to neurotoxin, could be investigated simultaneously. It was found that the neurotoxin MPP+ enabled to induce mobility defects and dopaminergic neurons loss in worms. The established system is easy and fast to operate, which offers not only the controllable microenvironment for analyzing the individual worms in parallel, monitoring the same worm over time, but also the capability to characterize the mobility behavior and neuron features in response to stimuli simultaneously. In addition, the device enabled to sustain the worm culture over most of their adult lifespan without any harm to worm, providing a potential platform for lifespan and aging research. 相似文献
14.
非接触式人体探测雷达利用雷达回波中包含人体的体动和心跳呼吸等生命特征信息,来探测隐藏于固定物后人体目标的存在。本文介绍了一种宽带线性调频连续波体制的非接触式雷达的信号检测方法,基于相谱分析来实现对目标的定位和运动参数提取。 相似文献
15.
We report a new design of microfluidic chip (Multiple electric Field with Uniform Flow chip, MFUF chip) to create multiple electric field strengths (EFSs) while providing a uniform flow field simultaneously. MFUF chip was fabricated from poly-methyl methacrylates (PMMA) substrates by using CO2 laser micromachining. A microfluidic network with interconnecting segments was utilized to de-couple the flow field and the electric field (EF). Using our special design, different EFSs were obtained in channel segments that had an identical cross-section and therefore a uniform flow field. Four electric fields with EFS ratio of 7.9:2.8:1:0 were obtained with flow velocity variation of only 7.8% CV (coefficient of variation). Possible biological effect of shear force can therefore be avoided. Cell behavior under three EFSs and the control condition, where there is no EF, was observed in a single experiment. We validated MFUF chip performance using lung adenocarcinoma cell lines and then used the chip to study the electrotaxis of HSC-3, an oral squamous cell carcinoma cell line. The MFUF chip has high throughput capability for studying the EF-induced cell behavior under various EFSs, including the control condition (EFS = 0). 相似文献
16.
A pulse compressor is utilized in many aspects of signal processing. Compression is usually accomplished by using special matched-filter-signal pairs. An alternative method takes advantage of the fact that any linear dynamic system can be excited to produce a time-limited output. Accordingly for a given dynamic system a synthesis procedure is provided for stepwise-constant input pulses evoking a time-limited response. Pairing the signal and system carefully leads to pulse compression. The Doppler invariant pulse compression is an important special case. Many examples are given. 相似文献
17.
针对常见的雷达脉冲信号（常规信号（NS）,线性调频信号（LFM）,双线性调频信号（DLFM）运用时频工具对调制方式的识别进行研究。运用两种时频分析方法1.短时傅里叶变换2.相位展开算法.通过这两种算法对雷达信号的调制方式进行分析,并通过仿真得到雷达信号的时--频分布以及时—频,时—相曲线,对仿真结果进行研究。通过仿真实验表明,雷达信号调制方式识别算法是可行的,有效的,而且具有良好的性能。 相似文献
18.
为了监测某区域某频率段内声波信号的强弱,提出一种以C8051F060为控制核心的声波信号采集系统的设计方案。采用压力场声波传感器将可识别的声波信号转换为适当幅度的电信号,该信号经过由程控滤波器地处理后直接送至带高速ADC的单片机进行处理,最后以SYG2401281图形点阵液晶为显示器件实现声波信号的实时显示。 相似文献
19.
Design of detectors for strong mixing signals in strong mixing noise is considered, where a large degree of dependency may occur between the signal and noise. Under the criterion of asymptotic relative efficiency, it is shown that this design reduces to determining the solution of an integral equation, where only knowledge of the second order statistics of the randon processes involved is required. In particular, if the signal is independent of the noise and has nonzero mean, the optimal detector is the same as in the known constant signal case. It is also shown that it is possible to delete several regularity conditions which may be difficult to check in practice in the slightly more restrictive case where the maximal correlation coefficients of the signal and noise tend to zero. 相似文献
20.
A microfluidic device integrated with 3D thin film microelectrode arrays wrapped around serpentine-shaped microchannel walls has been designed, fabricated and tested for cell electrofusion. Each microelectrode array has 1015 discrete microelectrodes patterned on each side wall, and the adjacent microelectrodes are separated by coplanar dielectric channel wall. The device was tested to electrofuse K562 cells under a relatively low voltage. Under an AC electric field applied between the pair of the microelectrode arrays, cells are paired at the edge of each discrete microelectrode due to the induced positive dielectrophoresis. Subsequently, electric pulse signals are sequentially applied between the microelectrode arrays to induce electroporation and electrofusion. Compared to the design with thin film microelectrode arrays deposited at the bottom of the side walls, the 3D thin film microelectrode array could induce electroporation and electrofusion under a lower voltage. The staggered electrode arrays on opposing side walls induce inhomogeneous electric field distribution, which could avoid multi-cell fusion. The alignment and pairing efficiencies of K562 cells in this device were 99% and 70.7%, respectively. The electric pulse of low voltage (～9 V) could induce electrofusion of these cells, and the fusion efficiency was about 43.1% of total cells loaded into the device, which is much higher than that of the convectional and most existing microfluidics-based electrofusion devices. 相似文献