共查询到19条相似文献，搜索用时 218 毫秒
1.
目的：探讨再生障碍性贫血患者治疗前后外周血T淋巴细胞亚群检测的变化及其在不同治疗中的意义。为合理治疗提供依据。方法：采用流式细胞技术检测40例再生障碍性贫血患者治疗前后外周血T淋巴细胞亚群检测的变化及CD4＋／CD8＋,并根据其比值与正常对照组比较将患者分为比值正常型、比值倒置和比值超高型3个免疫亚型。同时按常规治疗和联合免疫抑制治疗进行疗效比较。结果：单独常规治疗时。3个亚型组之间疗效无明显差异;联合免疫抑制治疗时,比值正常型患者的总有效率与常规治疗组无显著差异,而免疫倒置型和免疫异常型（比值减低和增高）患者总有效率较同组常规治疗疗效均显著提高（P〈0．05）,也显著高于比值正常者（P〈0．05）。结论：大多数再生障碍性贫血患者存在着CD4＋／CD8＋的失调。再生障碍性贫血患者的发病机制与免疫异常有密切的相关性。T淋巴细胞亚群检测对临床了解病情和发病机制、合理选择治疗方案、提高诊断和治疗水平都具有重要价值。 相似文献
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参麦注射液对树突状细胞免疫活性的影响 总被引：2，自引：0，他引：2
目的 研究参麦注射液对树突状细胞(DC)免疫活性的影响，并初步探讨其作用机理．方法 从人外周血分离单核细胞，对照组用GM-CSF和IL-4诱导DC，参麦组另加参麦注射液，两组均自第5天起加TNFa促成熟，倒置显微镜观察细胞形态；收集培养10天的DC用流式细胞仪做表型检测；用MTT法检测DC诱导T细胞增殖的作用；用ELISA法测定DC上清中IL-12分泌量．结果 外周血单核细胞经细胞因子及参麦注射液干预诱导10天，表现出典型DC形态，对照组和参麦组DC形态无差异；两组DC的CDla、CD40、CD80等表面分子表达无显著差异；参麦组DC诱导T细胞增殖的能力增强；参麦组DC分泌IL-12的量高于对照组．结论 参麦注射液对DC表面分子的表达无明显影响，但可增加IL-12的表达，增强DC的免疫活性． 相似文献
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目的:检测肺癌患者外周血中CK19-mRNA和MUC1-mRNA的表达情况,以探讨用于诊断非小细胞肺癌(nonsmallcelllungcancer,NSCLC)患者外周血微转移的临床意义。方法:采用逆转录(RT-PCR)方法,分别检测有明确肿瘤病灶的25非小细胞肺癌患者,20例肺部良性疾病患者和20例健康对照者外周血中CK19-mRNA和MUC1-mRNA的表达。结果25例肺癌患者外周血中CK19-mRNA和MUC1-mRNA表达阳性率分别为44.00%和56.00%,肺部良性疾病患者中CK19-mRNA有1例表达阳性(5%),而MUC1-mRNA则全为阴性;20例健康对照者两者皆表达为阴性。肺癌组与肺部良性疾病组、健康对照组间阳性率均有显著性差异(P<0.01)。结论CK19-mRNAandMUC1-mRNA作为外周血中肺癌的两项分子检测指标,是检测肺癌患者外周血微转移的较好标志物,具有一定的临床应用价值,值得进一步扩大样本研究及随访。 相似文献
4.
目的观察分析慢性病毒性肝炎患者应用大黄利胆合剂治疗的临床疗效。方法回顾性分析86例慢性病毒性肝炎患者临床资料,按治疗方法分为研究组(43例)和对照组(43例),两组患者均应用常规方法治疗,研究组在此基础上加以大黄利胆合剂,对照组加以强力宁注射液,对比观察两组患者临床疗效。结果研究组痊愈率、SB复常时间、ALT复常时间、HbeAg阴转率均优于对照组,差异有统计学意义(P0.05)。结论在常规方法治疗基础上,给予慢性病毒性肝炎患者大黄利胆合剂治疗,可可取得显著的临床疗效,能有效改善患者临床症状,促进早日康复。 相似文献
5.
CD14是单核巨噬细胞的表面标志（surface marker）．存在于95％的被激活的单核巨噬细胞表面，在大多数细胞激活及炎症反应过程中起关键作用。我们于2004年8月测量了无角多塞特羊与蒙古羊体内CD14＋的表达率。以了解这两种羊单核巨噬细胞系统吞噬能力的差异。 相似文献
6.
目的:观察逍遥散治疗慢性乙型病毒性肝炎的临床疗效。方法:100例慢性乙型病毒性肝炎患者随机分为两组,观察组50例在对照组基础上口服逍遥散,150ml/次,3次/d;对照组50例口服恩替卡韦分散片,0.5mg/次,1次/d。两组患者均治疗3个疗程共12周,观察两组患者治疗前后的临床症状、体征、HBV-DNA、肝功能的变化程度。结果:观察组患者临床疗效总有效率显著高于对照组(98%vs 84%),两组间比较差异有显著性意义(P0.05)。结论:加服逍遥散治疗慢性乙型病毒性肝炎有较好的疗效,值得临床进一步研究应用。 相似文献
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TH17细胞分化及其调控机制研究进展 总被引：2，自引：0，他引：2
在过去二十年里,对TH1和TH2细胞的分类构成了人们理解CD4+T细胞免疫学功能及它们在天然免疫和适应性免疫调节中作用的基础.近年来,新的免疫学理论认为初始T细胞可以在不同细胞因子及环境因素作用下分化为TH1、TH2、TH17和Treg细胞,几种效应细胞分别受到不同转录因子的调控,在体内产生不同的生物学功能.TH17细胞的发现更多的是与自身免疫性疾病的研究相关,它是T细胞分化过程中一种特殊的细胞亚群.初始T细胞在TGF-β和IL-6共同作用下分化为TH17细胞.同时它的分化受体内多种因素的调控.本文将从效应T细胞TH1和TH2、Treg细胞及DC细胞等对TH17细胞分化影响的角度分析TH17分化及其调控机制,这将对深入理解TH17细胞形成、分化和体内调控,并为自身免疫疾病和炎症冶疗提供一定的理论依据. 相似文献
9.
目的:探讨原发性肾病综合症患儿T细胞亚群变化的临床意义。方法:运用SPA花环法检测30原发性肾病综合症患儿活动期和缓解期T细胞亚群的变化。结果:活动期CD3 、CD4 、CD4/CD8低于缓解期,差异显著(P<0.01)与对照组比有统计学差异(P<0.01),缓解期与对照组无统计学差异(P>0.05)。结论:原发性肾病综合症患儿免疫功能低下,T细胞亚群可作为检测其变化的指标之一。 相似文献
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慢性支气管炎急性发作期患者T淋巴细胞亚群的研究 总被引：5，自引：1，他引：5
对慢性支气管炎急性发作期和缓解期患者体内T淋巴细胞亚群进行研究，用荧光单抗CD4^ 、C8^ /CD28^-,CD8^ /CD28^ 对T淋巴细胞亚群进行标记和计数。检测结果显示慢性支气管炎急性发作期患者体内CD4^ 无明显变化；CD8^ /CD28^-增加;CD8^ /CD28^ 减少。我们认为慢性支气管炎急性期患者T淋巴细胞亚群变化造成免疫功能紊乱。因此在治疗疾病过程中，除对症处理外，另须配合使用提高免疫功能药物，改善免疫功能。 相似文献
12.
Khan S Bhargava A Pathak N Maudar KK Varshney S Mishra PK 《Indian journal of clinical biochemistry : IJCB》2011,26(2):161-168
The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis of chronic hepatitis considered
a priority in clinical hepatology. Total viral load of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) patients
was quantified and correlation studies were performed with circulating levels of Th1/Th2 cytokines; C reactive protein and
circulating nucleosomes; glutathione reductase (GR) and superoxide dismutase. To our knowledge, the study is first among its
kind that validates strong positive correlation of viral load with IL-4, IL-6, GR in HBV and IL-6, IL-10, GR in HCV infections.
Although, multi-centric studies including large cohorts are required for translating our findings to clinical practice, however,
role of these biomarkers with potential diagnostic or prognostic significance might be helpful in clinical assessment of high-risk
individuals, thereby, designing interventional strategies, towards development of personalized medicare. The results of our
study also offer valuable insights of immune signaling mediators engaged in development of hepatocellular carcinoma. 相似文献
13.
Prasad P. Torkadi I. C. Apte A. K. Bhute 《Indian journal of clinical biochemistry : IJCB》2014,29(1):79-83
Alcoholic liver disease (ALD) is due to excessive alcohol intake for long duration. Distinguishing ALD from non-ALD (non-alcoholic steatohepatitis, hepatitis of viral origin) is difficult as patient may deny alcohol abuse. Clinical examination, histology and serology may not differentiate these conditions. Accurate diagnosis is important as management of ALD differs from non-ALD patients. The aim of our study was (1) To evaluate the patients of ALD and non-ALD by biochemical parameters compared to controls, (2) To assess whether these parameters can differentiate ALD from non-ALD. Study was carried out on 50 patients of ALD in group I and 35 patients of NASH (non-alcoholic steatohepatitis) and acute viral hepatitis each in group II. Age matched healthy controls n = 50. Selection criteria—history of alcohol intake (amount and duration), clinical examination, sonography of abdomen, serum alanine transaminase (ALT) and bilirubin levels. Blood samples were analyzed for bilirubin, aspartate transaminase (AST), ALT, alkaline phosphatase (ALP), gamma glutamyl transferase (GGT) by kinetic method. Statistical analysis was done by Student unpaired ‘t’ test. Patients of ALD have raised AST/ALT ratio (De Ritis ratio) (>2), ALP and GGT compared to controls (P < 0.01).There is significant difference in AST/ALT ratio, serum GGT and ALP in ALD group compared to that in NASH and acute viral hepatitis (P < 0.05). This study suggests that De Ritis ratio >2 in ALD patients may be due to alcohol induced hepatic mitochondrial injury and pyridoxine deficiency. High GGT and ALP values may indicate enzyme induction by alcohol and mild cholestasis. Thus ALD patients have severe hepatic damage. De Ritis ratio <1 and normal to mild elevation in GGT level in NASH and acute viral hepatitis suggest mild hepatic injury of non-alcoholic origin. Our study concludes that ALD patients can be differentiated from NASH and acute viral hepatitis with certainty by measuring serum AST/ALT ratio, GGT and ALP. These biochemical parameters may help clinicians to support the diagnosis of ALD and non-ALD. 相似文献
14.
Max M. Gong Brendan D. MacDonald Trung Vu Nguyen Kinh Van Nguyen David Sinton 《Biomicrofluidics》2013,7(4)
In this paper, we present a low cost and equipment-free blood filtration device capable of producing plasma from blood samples with mL-scale capacity and demonstrate its clinical application for hepatitis B diagnosis. We report the results of in-field testing of the device with 0.8–1 ml of undiluted, anticoagulated human whole blood samples from patients at the National Hospital for Tropical Diseases in Hanoi, Vietnam. Blood cell counts demonstrate that the device is capable of filtering out 99.9% of red and 96.9% of white blood cells, and the plasma collected from the device contains lower red blood cell counts than plasma obtained from a centrifuge. Biochemistry and immunology testing establish the suitability of the device as a sample preparation unit for testing alanine transaminase (ALT), aspartate transaminase (AST), urea, hepatitis B “e” antigen (HBeAg), hepatitis B “e” antibody (HBe Ab), and hepatitis B surface antibody (HBs Ab). The device provides a simple and practical front-end sample processing method for point-of-care microfluidic diagnostics, enabling sufficient volumes for multiplexed downstream tests. 相似文献
15.
Simin Sohrabi Azim Akbarzadeh Dariush Norouzian Ali Farhangi Mehri Mortazavi Mohammad Reza Mehrabi Mohsen Chiani Zahra Saffari Soheil Ghassemi 《Indian journal of clinical biochemistry : IJCB》2011,26(4):354-359
The attempt is made to produce recombinant factor VIII but the first step in producing such product is production and purification
of rabbit’s polyclonal antibody against factor VIII. The second and third steps involve monoclonal antibody and recombinant
factor VIII production. Factor VIII is one of the most important coagulating factor where its deficiency leads to diseases
like hemophilia type A or classic. It is an inherited disease. Previously, it was obtained through fractionation of blood
plasma of blood donors. After processing, factor VIII could be used to manage such patients. Due to transfer of viral disease
like hepatitis and HIV through factor VIII obtained by fractionation, high cost of production, insufficiency of the donors
and the process of virus removal, thus production of factor VIII through recombinant technology can be useful and helpful.
The reaction between antibody and antigen is one the most specific reaction; therefore, such reaction can be employed to identify
factor VIII. Thereby, rabbits were injected several times with adjuvant-linked antigen to produce antibody. The antibody was
separated from the blood sample, purified and used to identify factor VIII in the research. 相似文献
16.
采用ＰＳＭＴＥ７作为质粒,进行ＨＩＶ－１ｅｎｖ基因对ＣＤ４阳性单核细胞Ｕ９３７－２的导入与表达,ｅｎｖ基因导入细胞在微量重金属镉的诱导下,激活妄动子表达ｇｐ１２０及ｇｐ１６０经Ｗｅｓｔｅｒｎｂｌｏｔ转移显色后,可得清晰的ｅｎｖ目的的条带,此外,充式细胞仪对表达的ｇｐ１２０与ＣＤ４位点的结合作了分析,验证了该表达蛋白的结构与活性,从实验系可以观察到的表达ｇｐ１６０的ＣＤ４细胞株由于表达ｇｐ１６０而引 相似文献
17.
Emad F. Eskander Ahmed A. Abd-Rabou Shaymaa M. M. Yahya Ashraf El Sherbini Mervat S. Mohamed Olfat G. Shaker 《Indian journal of clinical biochemistry : IJCB》2013,28(4):348-357
The prevalence of hepatitis C virus (HCV) infection varies across the world, with the highest percent of infections reported in Middle East, increasingly in Egypt. The current study aimed at examining the bio-statistical correlation and multiple regression analyses of pituitary growth hormone (GH) and liver activities among HCV genotype-4 patients treated with PEG-IFN-α plus RBV therapy. Herein, the current study was conducted on 100 HCV genotype-4 infected patients and 50 healthy controls. Patients received PEG-IFN-α/RBV for 24 weeks. Host RNA was isolated from patients’ sera for HCV genotyping and viral load determination. Moreover, the enzymatic activities of the liver, AFP, GH, PT, and CBC were performed in all volunteers. The present study resulted that the activities of the hepatic enzymes among HCV genotype-4 patients correlated together significantly. While, human GH showed a significant positive regression with pre-treatment ALT concentration in responders. Furthermore, multiple regression analysis for GH showed a significant positive correlation with pre-treatment ALT in HCV genotype-4 infected patients. We concluded that there were a putative significant relation between GH and pre-treatment ALT activity in HCV infection and response to IFN-based therapy. 相似文献
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