An electrostatic microwell–based biochip for phytoplanktonic cell trapping     

Panwong Kuntanawat  Jirapat Ruenin  Rungrueang Phatthanakun  Phongsakorn Kunhorm  Werasak Surareungchai  Sompong Sukprasong  Nimit Chomnawang 《Biomicrofluidics》2014,8(3)

A simple microwell-based microfluidic chip for microalgal cells trapping was fabricated. An electrostatic cell trapping mechanism, enabled by a positively charged glass surface, was used. The chip was capable of capturing multiple algal cell types. In the case of filamentous Spirulina platensis, we observed single filament occupancy of up to ∼30% available wells, as high as some previously proposed methods. Captured filaments were not of any preferential size, suggesting well randomized cell trapping. It was found that the electrostatic attraction did not affect the cell growth. Total replacement of liquid inside the wells could be achieved by pumping new solutions via the inlet, making single cell experiments in controlled chemical conditions possible. After the top layer of the chip was removed, cells in the wells could be simply transferred using a micropipette, turning the chip into a platform for strain selection.  相似文献   

Solution pH change in non-uniform alternating current electric fields at frequencies above the electrode charging frequency     

Ran An  Katherine Massa  David O. Wipf  Adrienne R. Minerick 《Biomicrofluidics》2014,8(6)

AC Faradaic reactions have been reported as a mechanism inducing non-ideal phenomena such as flow reversal and cell deformation in electrokinetic microfluidic systems. Prior published work described experiments in parallel electrode arrays below the electrode charging frequency (f_c), the frequency for electrical double layer charging at the electrode. However, 2D spatially non-uniform AC electric fields are required for applications such as in plane AC electroosmosis, AC electrothermal pumps, and dielectrophoresis. Many microscale experimental applications utilize AC frequencies around or above f_c. In this work, a pH sensitive fluorescein sodium salt dye was used to detect [H⁺] as an indicator of Faradaic reactions in aqueous solutions within non-uniform AC electric fields. Comparison experiments with (a) parallel (2D uniform fields) electrodes and (b) organic media were employed to deduce the electrode charging mechanism at 5 kHz (1.5f_c). Time dependency analysis illustrated that Faradaic reactions exist above the theoretically predicted electrode charging frequency. Spatial analysis showed [H⁺] varied spatially due to electric field non-uniformities and local pH changed at length scales greater than 50 μm away from the electrode surface. Thus, non-uniform AC fields yielded spatially varied pH gradients as a direct consequence of ion path length differences while uniform fields did not yield pH gradients; the latter is consistent with prior published data. Frequency dependence was examined from 5 kHz to 12 kHz at 5.5 V_pp potential, and voltage dependency was explored from 3.5 to 7.5 V_pp at 5 kHz. Results suggest that Faradaic reactions can still proceed within electrochemical systems in the absence of well-established electrical double layers. This work also illustrates that in microfluidic systems, spatial medium variations must be considered as a function of experiment time, initial medium conditions, electric signal potential, frequency, and spatial position.  相似文献   

Formation of lipid bilayer membrane in a poly(dimethylsiloxane) microchip integrated with a stacked polycarbonate membrane support and an on-site nanoinjector     

Wei Teng  Changill Ban  Jong Hoon Hahn 《Biomicrofluidics》2015,9(2)

This paper describes a new and facile approach for the formation of pore-spanning bilayer lipid membranes (BLMs) within a poly(dimethylsiloxane) (PDMS) microfluidic device. Commercially, readily available polycarbonate (PC) membranes are employed for the support of BLMs. PC sheets with 5 μm, 2 μm, and 0.4 μm pore diameters, respectively, are thermally bonded into a multilayer-stack, reducing the pore density of 0.4 μm-pore PC by a factor of 200. The BLMs on this support are considerably stable (a mean lifetime: 17 h). This multilayer-stack PC (MSPC) membrane is integrated into the PDMS chip by an epoxy bonding method developed to secure durable bonding under the use of organic solvents. The microchip has a special channel for guiding a micropipette in the proximity of the MSPC support. With this on-site injection technique, tens to hundreds of nanoliters of solutions can be directly dispensed to the support. Incorporating gramicidin ion channels into BLMs on the MSPC support has confirmed the formation of single BLMs, which is based on the observation from current signals of 20 pS conductance that is typical to single channel opening. Based on the bilayer capacitance (1.4 pF), about 15% of through pores across the MSPC membrane are estimated to be covered with BLMs.  相似文献   

Hierarchical structural complexity in atomically precise nanocluster frameworks     

Xiao Wei  Xi Kang  Zewen Zuo  Fengqi Song  Shuxin Wang  Manzhou Zhu 《国家科学评论(英文版)》2021,8(3)

The supramolecular chemistry of nanoclusters is a flourishing area of nano-research; however, the controllable assembly of cluster nano-building blocks in different arrays remains challenging. In this work, we report the hierarchical structural complexity of atomically precise nanoclusters in micrometric linear chains (1D array), grid networks (2D array) and superstructures (3D array). In the crystal lattice, the Ag₂₉(SSR)₁₂(PPh₃)₄ nanoclusters can be viewed as unassembled cluster dots (Ag₂₉–0D). In the presence of Cs⁺ cations, the Ag₂₉(SSR)₁₂ nano-building blocks are selectively assembled into distinct arrays with different oxygen-carrying solvent molecules―Cs@Ag₂₉(SSR)₁₂(DMF)_x as 1D linear chains (Ag₂₉–1D), Cs@Ag₂₉(SSR)₁₂(NMP)_x as 2D grid networks (Ag₂₉–2D), and Cs@Ag₂₉(SSR)₁₂(TMS)_x as 3D superstructures (Ag₂₉–3D). Such self-assemblies of these Ag₂₉(SSR)₁₂ units have not only been observed in their crystalline state, but also in their amorphous state. Due to the diverse surface structures and crystalline packing modes, these Ag₂₉-based assemblies manifest distinguishable optical absorptions and emissions in both solutions and crystallized films. Furthermore, the surface areas of the nanocluster crystals are evaluated, the maximum value of which occurs when the cluster nano-building blocks are assembled into 2D arrays (i.e. Ag₂₉–2D). Overall, this work presents an exciting example of the hierarchical assembly of atomically precise nanoclusters by simply controlling the adsorbed molecules on the cluster surface.  相似文献   

Couple-stress Effects Near an Interior Hole of an Infinite Elastic Plane Subjected to a Concentrated Force     

Y.C. Hsu  W.J. Wang 《Journal of The Franklin Institute》1973,295(5):411-421

Couple-stress solutions are obtained near the hole boundary of an infinite elastic plane subjected to a radial concentrated force at x = b, y = 0,under the restriction of r/b<-0.2 and b/l?;-10. Here r and l are the distance from the center of the hole to the point of interest and a characteristic length (couple-stress constant). At r = a, θ = ±π2, the stress ratio of the couple-stress solution to the classical one for the current problem is slightly lower than one of the Mindlin problem (1) for some values of v, except for v = 0.5 where they are equal. The dimensionless stress σ&#x0302;_θθ is found along the hole boundary.  相似文献   

Periodic solutions for a kind of Rayleigh equation with two deviating arguments   总被引：2，自引：0，他引：2  

Lequn Peng  Qiyuan Zhou 《Journal of The Franklin Institute》2006,343(7):676-687

In this paper, we use the coincidence degree theory to establish new results on the existence of T-periodic solutions for the Rayleigh equation with two deviating arguments of the form