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1.
本文对伤寒LPS-PHA诊断试剂盒进行现场应用评价.来自各医院和不同地区977份不同类型的血清标本作LPS-PHA检测的同时与Widal试验作比较,结果伤寒沙门氏菌血培养阳性46份血清标本前者检出率(86.96％)显著高于后者(67.39％),两者在统计学上有显著差异(X~2=4.999,P<0.025),95份Widal试验阳性血清LPS-PHA阳性率为97.89％,漏检2份系乙型副伤寒抗体阳性者.607份对照血清标本LPS-PHA的假阳性率1.48％,低于Widal试验(4.84％).伤寒LPS-PHA的敏感度和特异度(94.33％和98.52％)高于Widal试验(89. 36％和95.72％).其他5项评价指标(阳性和阴性预示值,粗一致性,调整一致性和约登指数)亦都较Widal试验好.因此,LPS-PHA诊断试剂盒能满足目前临床上早期快速诊断伤寒的要求.但不适用于追溯诊断和血清流行病学调查.  相似文献   

2.
通过对军事理论考试系统的需求分析,使用ASP．NET2．0技术对系统进行设计,数据库管理系统使用SQL Server2000。本系统包括用户管理、权限管理、题库录入、成绩导出等模块的设计和实现。在微软VS2005开发平台上调试,使用C#语言及Visual Studio．NET工具组件完成系统开发。  相似文献   

3.
在电离层风暴期,现存的电离层F2层临界频率预测方法不能满足实际应用的要求。根据磁层ap系数和太阳黑子月均值作为风暴期训练序列,本文提出了一种基于神经网络的电离层F2层临界频率预测新方法。模拟结果表明,这种新方法比现有的预测方法（STORM模型和Cander提出的神经网络方法）具有更好的预测性能。  相似文献   

4.
The optimal control problems for a generalized vibration system based on the Conjugate Gradient Method is examined in the present study in estimating instantaneously the optimal control forces for a damped system such that the desire (or design) system displacements can be satisfied.The numerical experiments are performed to test the validity of the present algorithm by using three different types of desire system displacements. Results show that excellent estimations on the optimal control forces can be obtained simultaneously with arbitrary initial guesses within a couple of second's CPU time at Pentium III- PC.  相似文献   

5.
Detection of lgE and lgG antibodies in Aspergillosis is of diagnostic significance. The serological methods, such as agglutination, gel diffusion and counter immuno electrophoresis that are commonly used in the laboratories for diagnosis of Aspergillus induced infections, are less sensitive and high cross reactivity is often encountered. We carried out work on characterization and identification of diagnostically relevant antigens ofA. fumigatus. Well characterized antigens were used to develop an ELISA with 92% sensitivity and 89% specificity for detection of specific lgE and lgG in the sera of patients of allergic bronchopulmonary aspergillosis (ABPA), aspergilloma and invasive aspergillosis. Subsequently, a sample kit having “ready to use type” of dry reagents (powder/tableted buffers and lyophilized antigen, conjugate and reference sera) was formulated. The kit was validated with sera from patients of ABPA, related allergic disorders, tuberculosis, post-Kochs cases and thalassemic children receiving repeated blood transfusions. The performance of the kit was found to be satisfactory with coded sera.  相似文献   

6.
This paper offers a novel contribution to an evidence-based assessment of the attractiveness features (or perceived qualities) of cities or urban neighbourhoods, based on a quantitative evaluation of such areas by introducing and applying what is called ‘city-love’ analysis. To put this new concept in context, we offer first a concise overview of related and complementary notions (e.g. happiness, satisfaction, well-being, quality of life, contentment). Then we propose a new departure for attractiveness research pertaining to micro-based information on residents or users of cities by introducing the notion of a ‘city-love production function’. This function expresses the ability of cities to enhance the love or appreciation for a city or its neighbourhoods through an appropriate combination of five specific ‘city capital’ constituents. We test the validity of this so-called ‘Pentagon’ approach to city love by means of the city-love production function using a multivariate econometric model based on extensive heterogeneous statistical data on municipalities in Sweden and complemented with cell phone data. Our results are confronted with empirical ‘big data’ on the appreciation of Swedish places – and their characteristics – taken from social media platforms. The study offers also interesting findings from an advanced spatial-econometric and multilevel modelling approach. Our estimations show that the concept of the city-love production function allows us to quantitatively uncover important determinants of citizens’ love for their local environment.  相似文献   

7.
IntroductionWe investigated the interference of haemolysis on ethanol testing carried out with the Synchron assay kit using an AU680 autoanalyser (Beckman Coulter, Brea, USA).Materials and methodsTwo tubes of plasma samples were collected from 20 volunteers. Mechanical haemolysis was performed in one tube, and no other intervention was performed in the other tube. After centrifugation, haemolysed and non-haemolysed samples were diluted to obtain samples with the desired free haemoglobin (Hb) values (0, 1, 2, 5, 10 g/L). A portion of these samples was then separated, and ethanol was added to the separated sample to obtain a concentration of 86.8 mmol/L ethanol. After that, these samples were diluted with ethanol-free samples with the same Hb concentration to obtain samples containing 43.4, 21.7, and 10.9 mmol/L. Each group was divided into 20 equal parts, and an ethanol test was carried out. The coefficient of variation (CV), bias, and total error (TE) values were calculated.ResultsThe TE values of haemolysis-free samples were approximately 2-5%, and the TE values of haemolysed samples were approximately 10-18%. The bias values of haemolysed samples ranged from nearly - 6.2 to - 15.7%.ConclusionsHaemolysis led to negative interference in all samples. However, based on the 25% allowable total error value specified for ethanol in the Clinical Laboratory Improvement Amendments (CLIA 88) criteria, the TE values did not exceed 25%. Consequently, ethanol concentration can be measured in samples containing free Hb up to 10 g/L.  相似文献   

8.
Thyroglobulin autoantibodies (TgAb) are estimated to detect potential interferences in thyroglobulin (Tg) immunoassays and also for the diagnosis of autoimmune thyroid disease. A user friendly and robust in-house solid-phase radioassay was standardized and parameters like sensitivity, reproducibility and stability were assessed. Further, it was validated and evaluated for the detection of autoantibodies in differentiated thyroid cancer (DTC) patients. Totally 301 samples received in our laboratory for routine serum Tg estimation were studied. The samples were analyzed for TgAb by the solid-phase radioassay developed in-house and compared with commercial anti-hTg IRMA kit (Immunotech, France). The control group comprised of 37 euthyroid males from our Centre. The intra- and inter-assay CVs for the two quality control samples (Control A = 104 ± 12.6 IU/mL and Control B = 1029 ± 114 IU/mL) were found less than or equal to 6.05 and 13.85 % respectively. Solid-phase radioassay showed a good agreement on comparison with Immunotech IRMA (r = 0.99). Using the proposed cut-off thresholds (in-house solid-phase radioassay 52 IU/mL and Immunotech IRMA 30 IU/mL), 5.4 % of the control subjects were positive for TgAb by both the methods. Prevalence of TgAb in DTC patients was 17.3 and 16.6 % using the Immunotech kit and in-house solid-phase radioassay respectively. The in-house solid-phase radioassay has the requisite sensitivity for the evaluation of TgAb comparable to commercial kit and also suitable for routine use as it is rapid, user friendly and economical.  相似文献   

9.
The comparative diagnostic efficacy of two cardiac markers: CK-MB and cTn-T, has scarcely been investigated in Indian patients of acute myocardial infarction. The present study was conducted for the same objective. The present study comprised of 59 patients. Males were 44 (75%) and females were 15 (25 %). The age of patients ranged from 32–84 years with mean age of 62.8 yrs. The mean age of males and females were 60 and 63 yrs respectively. All patients presented with history of chest pain with a 12 leads ECG proven MI (ST Elevation, discordant T-waves). CK-MB was estimated in peripheral blood samples at 0,24,48 and 72 hours by an autoanalyzer. Following 12 hours of admission bed side Troponin-T test was done employing cTn-T marker kit. Initially (0 hr), in 50% patients CK-MB was elevated. By end of 24 hours all the patients were CKMB positive and peak level was attained at 24 hrs. Then it tended to decline over next 48 hrs. There were no false positive or negative results. The cTn-T test was positive only in 22 % of ECG positive infarctions. However, the cTn-T positive cases were always accompanied by a higher CK-MB levels. A significantly lower cTn-T positive cases in Indian patients can only be attributed to some difference in amino acid sequence of Indian cTn-T and occidental cTn-T. A larger study from other Indian cardiac centers can either substantiate or contradict our results.  相似文献   

10.
The anonymization of query logs is an important process that needs to be performed prior to the publication of such sensitive data. This ensures the anonymity of the users in the logs, a problem that has been already found in released logs from well known companies. This paper presents the anonymization of query logs using microaggregation. Our proposal ensures the k-anonymity of the users in the query log, while preserving its utility. We provide the evaluation of our proposal in real query logs, showing the privacy and utility achieved, as well as providing estimations for the use of such data in data mining processes based on clustering.  相似文献   

11.

Introduction

Nowadays, on-a-chip capillary electrophoresis is a routine method for the detection of PCR fragments. The Agilent 2100 Bioanalyzer was one of the first commercial devices in this field. Our project was designed to study the characteristics of Agilent DNA 1000 kit in PCR fragment analysis as a part of circulating tumour cell (CTC) detection technique. Despite the common use of this kit a complex analysis of the results from a long-term project is still missing.

Materials and methods

A commercially available Agilent DNA 1000 kit was used as a final step in the CTC detection (AdnaTest) for the determination of the presence of PCR fragments generated by Multiplex PCR. Data from 30 prostate cancer patients obtained during two years of research were analyzed to determine the trueness and precision of the PCR fragment size determination. Additional experiments were performed to demonstrate the precision (repeatability, reproducibility) and robustness of PCR fragment concentration determination.

Results

The trueness and precision of the size determination was below 3% and 2% respectively. The repeatability of the concentration determination was below 15%. The difference in concentration determination increases when Multiplex-PCR/storage step is added between the two measurements of one sample.

Conclusions

The characteristics established in our study are in concordance with the manufacturer’s specifications established for a ladder as a sample. However, the concentration determination may vary depending on chip preparation, sample storage and concentration. The 15% variation of concentration determination repeatability was shown to be partly proportional and can be suppressed by proper normalization.Key words: lab-on-a-chip devices, capillary electrophoresis, multiplex PCR, circulating tumour cells, Agilent DNA 1000 kit  相似文献   

12.
In most cases, empirical analyses of environmental innovations based on firm-level data relied on surveys for one point in time. These surveys allow for the inclusion of many explanatory variables such as different policy instruments or the influence of pressure groups but it is not possible to address the dynamic character of environmental innovations. This paper uses two German panel databases, the establishment panel of the Institute for Employment Research (IAB) and the Mannheim innovation panel (MIP) of the Centre for European Economic Research (ZEW), to explore the determinants of environmental innovations. The econometric estimations show that the improvement of the technological capabilities (“knowledge capital”) by R&D triggers environmental innovations. Environmental regulation, environmental management tools and general organizational changes also encourage environmental innovation, a result that has also been postulated by the famous Porter-hypothesis.  相似文献   

13.
Document filtering (DF) and document classification (DC) are often integrated together to classify suitable documents into suitable categories. A popular way to achieve integrated DF and DC is to associate each category with a threshold. A document d may be classified into a category c only if its degree of acceptance (DOA) with respect to c is higher than the threshold of c. Therefore, tuning a proper threshold for each category is essential. A threshold that is too high (low) may mislead the classifier to reject (accept) too many documents. Unfortunately, thresholding is often based on the classifier's DOA estimations, which cannot always be reliable, due to two common phenomena: (1) the DOA estimations made by the classifier cannot always be correct, and (2) not all documents may be classified without any controversy. Unreliable estimations are actually noises that may mislead the thresholding process. In this paper, we present an adaptive and parameter-free technique AS4T to sample reliable DOA estimations for thresholding. AS4T operates by adapting to the classifier's status, without needing to define any parameters. Experimental results show that, by helping to derive more proper thresholds, AS4T may guide various classifiers to achieve significantly better and more stable performances under different circumstances. The contributions are of practical significance for real-world integrated DF and DC.  相似文献   

14.
高分辨率全帧CCD相机电路系统的设计   总被引：4，自引：0，他引：4  
基于Dalsa公司的面阵CCD评价板EKxxxx设计了一套高分辨率全帧CCD相机电路系统。系统采用专用集成芯片SAA8103和TDA9991构成时序发生器和驱动器，采用TDA9965进行前端信号处理。采用单片机对系统进行控制并应用Camera Link接口简化了体系结构。针对CCD多路输出时各通道存在不均匀性，提出采用两点校正法进行校正。该系统能以较高的帧频获取超高分辨率的图像，适用于航空拍摄、数字后背等场合。  相似文献   

15.
农业生态系统地下水氮面源污染数值模拟   总被引：1，自引：0，他引：1  
结合农业生态系统特征,以实例展示了在仅有短期有限野外监测数据及少量野外试验条件下,通过合理概化、参数分步识别与过程综合分析等,使模型识别的可靠性提高,成功地建立了农业生态系统地下水氮面源污染数值模型,并反演出表征农业氮面源污染特征的地下水入渗补给强度和氮补给量.本研究对定量评价农业生态系统地下水氮面源污染,特别是对野外基本调查工作部署与根据有限的基本数据较好地模拟识别特征参数,具有一定借鉴意义.  相似文献   

16.
We present an integrated microfluidic device capable of performing single-stranded DNA (ssDNA) preparation and magnetic bead-based microarray analysis with a white-light detection for detecting mutations that account for hereditary hearing loss. The entire operation process, which includes loading of streptavidin-coated magnetic beads (MBs) and biotin-labeled polymerase chain reaction products, active dispersion of the MBs with DNA for binding, alkaline denaturation of DNA, dynamic hybridization of the bead-labeled ssDNA to a tag array, and white-light detection, can all be automatically accomplished in a single chamber of the microchip, which was operated on a self-contained instrument with all the necessary components for thermal control, fluidic control, and detection. Two novel mixing valves with embedded polydimethylsiloxane membranes, which can alternately generate a 3-μl pulse flow at a peak rate of around 160 mm/s, were integrated into the chip for thoroughly dispersing magnetic beads in 2 min. The binding efficiency of biotinylated oligonucleotides to beads was measured to be 80.6% of that obtained in a tube with the conventional method. To critically test the performance of this automated microsystem, we employed a commercial microarray-based detection kit for detecting nine mutation loci that account for hereditary hearing loss. The limit of detection of the microsystem was determined as 2.5 ng of input K562 standard genomic DNA using this kit. In addition, four blood samples obtained from persons with mutations were all correctly typed by our system in less than 45 min per run. The fully automated, “amplicon-in-answer-out” operation, together with the white-light detection, makes our system an excellent platform for low-cost, rapid genotyping in clinical diagnosis.  相似文献   

17.
Metabolic syndrome (MetS) is a cluster of interrelated common clinical disorders. The role of resistin in insulin sensitivity and MetS is controversial till date. So, the aim of the present study was to investigate the relationship of plasma resistin levels with markers of the MetS in Indian subjects. In a case control study, total 528 subjects were selected for the study. 265 (194 male and 71 female) were cases (with MetS) and 263 (164 male and 99 female) were controls (without MetS). Required anthropometric measurements and calculations were carried out accordingly. All the Biochemical estimations were carried out according to standard protocol. Resistin level was measured by the standard protocol (By ELISA i.e. enzyme linked immunosorbent assay) as illustrated in the kit. Insulin level was also measured by the standard protocol as illustrated in the kit and insulin resistance was calculated by the standard procedures. Plasma resistin levels were significantly higher in cases compared with controls (male = 13.05 ± 4.31 vs. 7.04 ± 2.09 ng/ml; p ≤ 0.001 and female = 13.53 ± 4.14 vs. 7.42 ± 2.30 ng/ml; p ≤ 0.001). Plasma resistin levels were well correlated with waist circumference, glucose, triglycerides, waist/hip ratio, systolic and diastolic blood pressure, high density lipoprotein, total cholesterol, serum low density lipoprotein, serum very low density lipoprotein, insulin and insulin resistance. Plasma resistin levels were elevated in presence of the MetS and were associated with increased metabolic risk factors.  相似文献   

18.
The negative interference by bilirubin in serum creatinine estimation by Jaffe’s kinetic method is well known. Several approaches have been suggested to overcome this interference. In this article three different creatinine kits (Jaffe’s kinetic method) have been tested for bilirubin interference and its rectification using two simple approaches. The performance of three kits (A, B and C) supplied by three different manufacturers was tested using IQC and EQAS sera and pooled serum with added bilirubin. To overcome the bilirubin interference two approaches viz. NaOH preincubation and TCA precipitation were used. Bilirubin did interfere in creatinine estimation after a certain level (2.3 mg/dl). However, both NaOH preincubation and TCA precipitation approach rectified this interference. The performance of kit A was better than kit B and C. All the three kits showed bilirubin interference upon increasing the bilirubin concentration but kit A performed better than kit B and C. However, NaOH incubation and TCA precipitation methods overcame this interference to a great extent.  相似文献   

19.
Current recommendations of the Adult Treatment Panel and Adolescents Treatment Panel of National Cholesterol Education Program make the low-density lipoprotein cholesterol (LDL-C) levels in serum the basis of classification and management of hypercholesterolemia. A number of direct homogenous assays based on surfactant/solubility principles have evolved in the recent past. This has made LDL-C estimation less cumbersome than the earlier used methods. Here we compared one of the direct homogenous assays with the widely used Friedewald’s method of estimation of LDL-C to see the differences and correlation. We used direct homogenous assay kit to estimate serum LDL-C and high-density lipoprotein cholesterol (HDL-C). Serum Triglyceride (TG) and Total Cholesterol (TC) was estimated and using Friedewald’s formula LDL-C was calculated. The LDL-C level obtained by both methods in 893 fasting serum samples were compared. The statistical methods used were paired t-test and Pearson’s correlation. There was significant difference in the mean LDL-C levels obtained by the two methods at the TG levels <200 mg/dl (p<0.02) and TC levels >150 mg% (p<0.001). The correlation coefficient (r) between Friedewald’s and direct assay estimation was 0.88. Friedewald’s method classified 23.5 % of patients as high cardiac risk whereas there were 17.58% by direct assay. Both had good correlation even though the serum triglyceride and total cholesterol levels affect the difference in LDL-C estimated by both methods. Taking into account the cost and performance, Friedewald’s method is as good or even better for classifying and managing patients.  相似文献   

20.
Viterbo P 《Endeavour》2004,28(1):30-35
Gershwin's song 'I Got Rhythm' serves here as a backdrop representing the social context of the inter-war years. On center stage is a particular aspect of the history of birth control--the application of a new theory of ovulation to contraception. Starting in 1928, a series of experiments revealed a biochemical rhythm in the female reproductive cycle, which contradicted the widespread idea that ovulation and pregnancy could occur at any time. This discovery was applied to a new contraceptive method, the rhythm method, which enjoyed significant popularity during the 1930s, especially among Catholics. For a short period, women could join Ethel Merman in the refrain 'I got rhythm, I got my man, who could ask for anything more?' But the rhythm method has not lived to its promise, and the play goes on em leader  相似文献   

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