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淋巴细胞亚群的功能及其辐射效应 总被引：1，自引：0，他引：1
应用淋巴细胞单克隆抗体(McAb)的Panning法或亲和层析分纯 CD_4~+、CD_8~+、B细胞和非CD_4~+细胞,也应用PHA、ConA、PWM和LPS分别激活淋巴细胞,然后用~3H-TdR按入、集落形成及羟基磷灰石方法,探讨了各亚群淋巴细胞的调节功能及其辐射效应.实验结果表明PWM能激活CD_4~+、非CD_4~+和B细胞,PWM细胞能诱导LPS细胞转化,其中一种细胞受照即失去协同作用,PWM细胞受照影响更严重.ConA能激活CD_4~+和CD_8~+细胞,两者均可抑制LPS细胞转化,并能互相增强抑制效应,受照后消失.辐射敏感性比较:ConA细胞和PHA细胞大于PWM细胞,PWM细胞大于LPS细胞,CD_8~+细胞和B细胞大于CD_4~+细胞。 相似文献
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P. Subash 《Indian journal of clinical biochemistry : IJCB》2016,31(2):185-193
The objective of the present study was to investigate the antioxidant status and the extent of oxidative DNA damage in lymphocytes and their relation with essential hypertension (EHT). A total of 100 South Indian subjects aged 30–65 were included for the study. Of these 50 were normotensive controls (group-1) with blood pressure ≥120/80 mm Hg, 50 were newly diagnosed (group-2) and were not on any antihypertensive drugs, but had systolic blood pressure ranging between 140 and 160 mmHg and diastolic blood pressure 95–100 mmHg and 50 newly diagnosed essential hypertensive patients underwent drug therapy for 1 year was considered as group-3. Enzymatic and non-enzymatic antioxidants significantly decreased and lymphocyte DNA damage was significantly increased in newly diagnosed hypertensive patients compared with control group. The major decrease in DNA damage and significant improvement in enzymatic and non-enzymatic antioxidants were observed after 1 year of antihypertensive therapy in treated group compared with newly diagnosed hypertensive patients. Total antioxidant status and lymphocyte DNA damage showed a strong negative correlation in all the three groups. Essential hypertension associated with oxidative stress which in turn causes genotoxic susceptibility to variety of disease including cancer. In the absence of DNA repair process and DNA checkpoint mechanisms, the genomic integrity is susceptible to extensive damage. In our study, increased oxidative DNA damage and decreased antioxidant levels were frequently observed in the newly diagnosed essential hypertensive patients, suggesting that oxidative stress is important in the pathogenesis of EHT. Therefore, the present study has additional clinical implication. Further investigations with large number of patients along with antioxidant supplement are highly warranted. 相似文献
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重症肺炎患者免疫功能的初步研究 总被引：1，自引：0，他引：1
目的 对重症肺炎患者的免疫功能变化进行研究。方法 用荧光单抗CD3^ ,CD4^ ,CD25^ ,CD95^ ,CD3^ /CD25^ ,CD8^ /CD28^ ,CD8^ /CD28^-,CD19^ 对淋巴细胞标记和计数。结果 重症肺炎患者体内CD3^ ,CD95^ ,CD3^ /CD25^ ,CD8^ /CD28^ 减少，CD4^ ,CD25^ ,CD19^ 无明显变化，而CD8^ /CD28^-增加。结论 重症肺炎患者B淋巴细胞变化不大，T淋巴细胞因大量凋亡而总数减少，亚群的变化又造成免疫功能紊乱，在临床治疗过程中需改善患者免疫功能。 相似文献
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Ravichandran M. Regunathan J. Narayanan R. B. Kunthala Jayaraman Kaliraj P. 《Indian journal of clinical biochemistry : IJCB》1997,12(1):27-31
Human lymphatic filariasis is a chronic helminth infection caused byWuchereria bancrofti andBrugia malayi. Wide spectrum of clinical manifestations are seen in different clinical groups of filariasis patients which comprises asymptomatic patients with circulating microfilaria (MF), individuals with chronic lymphatic obstruction (CP), Endemic normals (EN) who are asymptomatic and amicrofilaraemic or the relatively rare tropical pulmonary eosinophilia (TPE). The cellular immune response to this infection varies in different clinical groups of filarial patients. ranging from normal lymphocyte proliferative response in EN individuals to lymphocyte hyporesponsiveness in MF to total parasite antigen [Brugia malayi antigen (BMA)]. But in response to recombinant filarial antigen (pRJ51) the lymphocyte proliferation is restored in MF patients. Interestingly the lymphocytes from MFs responded normally to parasite antigen when EN serum was added in the culture whereas sera from MFs failed to revert the lymphocyte hyporesponsiveness. In order to study the molecular mechanisms responsible for parasite antigen specific lymphocyte hyporesponsiveness, we analysed both the Th1 and Th2 type cytokine gene expression profile in different clinical groups of filarasis patients. MF individuals expressed elevated Th2 type cytokines like IL-4, IL-5 and IL-10 and decreased levels of IL-2 and IFN-γ in response to parasite antigen. Chronic patients have elevated levels of both Th1 and Th2 type cytokines in response to parasite antigen. The EN individuals had a purely Th1 type pattern with absence of IL-4 and IL-5 expression. These studies clearly demonstrate the role of Th2 cytokine like IL-10 in antigen specific hyporesponsiveness seen in MF patients. Any methods to arrest the progression of this disease should concentrate on the means to revert the Th2 type into Th1 type response in the MF patients either by Th1 type cytokine therapy or by using recombinant filarial antigen which stimulates the Th1 response. Further the recombinant filarial antigen which induces Th1 type cytokine response could be used for immunoprophylactic studies. 相似文献
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赵喜纯 《科技成果管理与研究》2011,(6):86-88
本文从六个方面探讨了欧盟国家的节能减排技术：欧盟国家的垃圾处理技术、欧盟国家污水处理厂及污泥无害化处理技术、城市水资源保护与再利用、天然水激活技术以及城市建筑的节能技术。认为应将欧盟的节能环保技术引进中国,以使中国在几年之内达到他们40多年的发展进度。 相似文献
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R. Kaleysa Raj C. G. Pradeep C. Maya Devi Linda John M. R. Rekha Sheeba K. Oommen 《Indian journal of clinical biochemistry : IJCB》1997,12(1):32-34
ES materials released along with the release of mf showed immunosuppression as evidenced by a decrease in T lymphocyte levels in the immunised animals. Lipid fractions of the mf associated ES materials and protein fractions of the detergent soluble materials were found to cause suppression of immune response in the host. Further study revealed that the suppression associated with the lipid fractions is actually the end result and that mf associated ES materials is actually a mixture of immunosuppressive and immunopotentiating components and the release of the components is most likely regulated. 相似文献
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Yasemin U Budak Ka?an Huysal Hakan Demirci 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(1):97-102
Background
In heart failure patients, mean platelet volume (MPV) may reflect increased platelet activation or increased numbers of large, hyper-aggregable platelets. B-type natriuretic peptide (BNP) concentration in blood is a sensitive and specific marker of heart failure, correlating with the severity and prognosis of illness, in patients presenting with acute dyspnea to the emergency department. This study evaluated the correlation between BNP concentration and MPV.Material and methods
Data were collected from 319 patients admitted to the emergency department of a cardiology hospital from January–July 2014. EDTA blood samples drawn at admission were analyzed using automated hematology system, and BNP concentration was measured using a fluorescence immunoassay.Results
The study included 190 patients with and 129 without acute heart failure (AHF). These groups had BNP concentration of 200-5000 ng/L and 5-98 ng/L, respectively. MPV levels were significantly higher in the AHF group (P < 0.001). BNP concentrations were positively correlated with MPV (r = 0.41, P < 0.001) and neutrophil / lymphocyte ratio (r = 0.38, P < 0.001).Conclusion
Increased MPV values correlate with BNP concentration, an indicator of HF severity and clinical status, in patients with AHF admitted to the emergency department.Key words: natriuretic peptide, brain; heart failure; platelet; mean platelet volume 相似文献10.
《Information processing & management》2005,41(5):1081-1099
Many segments of modern society, including marketing, politics, government, activism and public safety, desire the ability to find relationships, thus meaning, in public discourse. This can be accomplished by analyzing communication documents according to their content. The increasing use of the Internet for public dialog has made Internet communication a potentially rich source of data in this regard. This study explores the use of an interactive activation with competition (IAC) artificial neural network (ANN) to find relationships in email texts. A modified fully recurrent IAC network was used to process 69 email messages from two threads in the Open Library/Information Science Education Forum using two variations of the self-organizing phase of network formation. These variations were: (1) with and (2) without a linear decay function applied between sentences to the external activation of nodes. The use of the linear decay function, which could be considered a method for including context, produced three positive effects: the entire network was more differentiated from keywords; the keywords were more highly associated with each other, and; roughly half the number of noise strings were highly associated with keywords. 相似文献
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为了提高数学课堂教学效率,教师要不断更新教育观念,提高业务水平,掌握科学的教育规律,有效激活课堂教学,充分利用课堂资源,最大限度调动学生兴趣,积极参与、体验,以达到对新知识的理解和掌握,从而落实课堂教学的高效率。 相似文献
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《Journal of The Franklin Institute》2023,360(2):1344-1377
A novel finite-time complex-valued zeroing neural network (FTCVZNN) for solving time-varying Sylvester equation is proposed and investigated. Asymptotic stability analysis of this network is examined with any general activation function satisfying a condition or with an odd monotonically increasing activation function. So far, finite-time model studies have been investigated for the upper bound time of convergence using a linear activation function with design formula for the derivative of the error or with variations of sign-bi-power activation functions to zeroing neural networks. A function adaptive coefficient for sign-bi-power activation function (FA-CSBP) is introduced and examined for faster convergence. An upper bound on convergence time is derived with the two components in the function adaptive coefficients of sign-bi-power activation function. Numerical simulation results demonstrate that the FTCVZNN with function adaptive coefficient for sign-bi-power activation function is faster than applying a sign-bi-power activation function to the zeroing neural network (ZNN) and the other finite-time complex-valued models for the selected example problems. 相似文献
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A. Madhavi Reddy Y. N. Rao Yogendra Singh Alpana Saxena 《Indian journal of clinical biochemistry : IJCB》1995,10(2):66-71
Adenosine deaminase and protein tyrosine phosphatase activities in liver and peritoneal macrophages were estimated in control,
streptozotocin induced diabetes and insulin treated diabetic groups of Swiss albino mice. Both the enzyme levels were elevated
while glycogen content decreased in the liver of diabetic group of animals as compared to controls, while in insulin treated
group, they were comparable to control levels. In macrophages also, both adenosine deaminase which plays an important role
in their maturation and protein tyrosine phosphatase enzyme involved in their activation were high in diabetic group, whereas
in insulin treated diabetic group, the values were comparable to control levels. *** DIRECT SUPPORT *** A00FN011 00003 相似文献
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Ca++ was not found to be essential for the activation of the latent elastase in normal lenses. However, the activities increased 70–100% in human, bovine, sheep and goat lenses at a concentration of 5.0×10?3M of CaCl2. On the other hand, in cataractous human lenses, Ca++ inhibited the process of activation. The activation process however, seems to be a divalent metal ion dependent process since ethylene diamine tetra acetate (2.0×10?3M) blocked the activation of the elastase-like enzyme in both normal and cataractous lenses. p-Hydroxy mercuribenzoate (10?4M) and benzamidine (1.65×10?2M) also blocked the activation process, indicating that a trypsin-like enzyme requiring sulfhydryl groups, is involved in the activation of latent elastase. 相似文献
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Manzoor Ahmad Nida Suhail Tariq Mansoor Naheed Banu Shamshad Ahmad 《Indian journal of clinical biochemistry : IJCB》2012,27(4):385-388
In the present study, oxidative stress and lymphocytic DNA damage in both pre-op and post-op benign prostrate hyperplasia (BPH) patients with age >50 years was evaluated and compared with normal healthy subjects (controls- without any evidence of disease) of the same sex and age group. From December 2007 to November 2009, oxidative stress in 45 BPH patients were evaluated both before (pre-op patients) and after 7 days of surgery (post-op patients) in terms of measurements of plasma levels of (1) various anti-oxidative enzymes, (2) non-enzymatic antioxidants and (3) malondialdehyde which is a product of lipid peroxidation. The lymphocyte DNA damage was also evaluated by single cell alkaline gel electrophoresis in terms of tail length migration in these patients. These values were compared with their respective control subjects of similar sex and age group. The activities of antioxidant enzymes and the levels of antioxidant, reduced glutathione were found significantly decreased (p < 0.05) in serum samples of pre-operative group of BPH patients as compared to the controls. These altered parameters increased significantly (p < 0.05) and returned to their near normal control values, but not up to baseline values, in post operative patients i.e. after the cancer load was decreased by surgery. Lymphocytic DNA damage was found to be significantly increased in pre-op group as compared to controls and was reduced after surgery in post-op group. The present study therefore, shows significantly increased levels of oxidative stress and DNA damage in BPH patients which were reduced after removal of tumour load. Thus oxidative damage plays an important role in prostate tumourogenesis and timely management of oxidative stress can be of importance in preventing the occurrence of BPH. 相似文献
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以溶解或循环形式存在于机体血清及其它体液中的可溶性细胞间粘附分子-1(sICAM-1)的重要生物学功能是,与膜型ICAM-1竞争性地结合白细胞膜表面的LFA-1、Mac-1等分子,参与机体的免疫调节,从而与肿瘤的发生、发展和转归密切相关。本文综述了sICAM-1的来源、结构、功能及其与肺癌的相互关系。 相似文献
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Wei-Guang Li Yan-Jiao Wu Xue Gu Hui-Ran Fan Qi Wang Jia-Jie Zhu Xin Yi Qin Wang Qin Jiang Ying Li Ti-Fei Yuan Han Xu Jiangteng Lu Nan-Jie Xu Michael Xi Zhu Tian-Le Xu 《国家科学评论(英文版)》2021,8(9)
Synaptic associativity, a feature of Hebbian plasticity wherein coactivation of two inputs onto the same neuron produces synergistic actions on postsynaptic activity, is a primary cellular correlate of associative learning. However, whether and how synaptic associativity are implemented into context-dependent relapse of extinguished memory (i.e. fear renewal) is unknown. Here, using an auditory fear conditioning paradigm in mice, we show that fear renewal is determined by the associativity between convergent inputs from the auditory cortex (ACx) and ventral hippocampus (vHPC) onto the lateral amygdala (LA) that reactivate ensembles engaged during learning. Fear renewal enhances synaptic strengths of both ACx to LA and the previously unknown vHPC to LA monosynaptic inputs. While inactivating either of the afferents abolishes fear renewal, optogenetic activation of their input associativity in the LA recapitulates fear renewal. Thus, input associativity underlies fear memory renewal. 相似文献
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Aman Kumar Niti Kumari Vinny Gupta Rajendra Prasad 《Indian journal of clinical biochemistry : IJCB》2018,33(3):246-254
Renal cell carcinoma is the most common form of the kidney cancer accounting for more than 85% of the cases of which clear cell renal cell carcinoma (ccRCC) is the major histological subtype. The central molecular signature for ccRCC pathogenesis is the biallelic inactivation of VHL gene due to the presence of mutations/hyper-methylation/complete gene loss, which results in the downstream HIF activation. These events lead to increased tyrosine kinase receptor signalling pathways (RAS/MEK/ERK pathway, PI3K/AKT/mTOR pathway and NF-κB pathway), which through their downstream effector proteins causes the cell to proliferate and migrate. Recent studies have shown that VHL inactivation alone is not sufficient to induce the tumor. Mutations in numerous other genes that codes for chromatin modifiers (PBRM1, SETD2 and BAP1) and signalling proteins (PTEN and mTOR) have been identified along with activation of alternate signalling pathways like STAT and Sonic Hedgehog (SHH) pathway. It has also been shown that STAT pathway also works cooperatively with HIF to enhance the tumor progression. However, SHH pathway reactivation resulted in tumor regardless of the VHL status, indicating the complex nature of the tumor at the molecular level. Therefore, understanding the complete aetiology of ccRCC is important for future therapeutics. 相似文献
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In this paper we study stochastic stability of delayed recurrent neural networks with both Markovian jump parameters and nonlinear disturbances. Based on the Lyapunov stability theory, the properties of a Brownian motion, the generalized Itô's formula and linear matrix inequalities technique, some new delay-dependent conditions are derived to guarantee the stochastically asymptotic stability of the trivial solution or zero solution. In particular, the activation functions in this paper depend on Markovian jump parameters and they are more general than those usual Lipschitz conditions. Also, time delays proposed in this paper comprise both constant delays and time-varying delays. Moreover, the derivative of time delays is allowed to take any value. Therefore, the results obtained in this paper are less conservatism and generalize those given in the previous literature. Finally, two numerical examples and their simulations are used to show the effectiveness of the obtained results. 相似文献
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In a crystalline material, structural defects such as dislocations or twins are well defined and largely determine the mechanical and other properties of the material. For metallic glass (MG) with unique properties in the absence of a long-range lattice, intensive efforts have focused on the search for similar ‘defects’. The primary objective has been the elucidation of the flow mechanism of MGs. However, their atomistic mechanism of mechanical deformation and atomic flow response to stress, temperature, and failure, have proven to be challenging. In this paper, we briefly review the state-of-the-art studies on the dynamic defects in metallic glasses from the perspective of flow units. The characteristics, activation and evolution processes of flow units as well as their correlation with mechanical properties, including plasticity, strength, fracture, and dynamic relaxation, are introduced. We show that flow units that are similar to structural defects such as dislocations are crucial in the optimization and design of metallic glassy materials via the thermal, mechanical and high-pressure tailoring of these units. In this report, the relevant issues and open questions with regard to the flow unit model are also introduced and discussed. 相似文献