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1.
C. E. J. Udiong A. E. Udoh M. E. Etukudoh 《Indian journal of clinical biochemistry : IJCB》2007,22(2):74-78
The prevalence of abnormal thyroid hormone levels in diabetes mellitus in Nigeria is not well described. To determine the
incidence of abnormal thyroid hormone levels in diabetics in Calabar, Nigeria, fasting blood samples from 161 diabetic subjects
and 105 non-diabetic controls were analysed. Free thyroxine (FT), thyroid stimulating hormone (TSH), total triiodothyronine
(T3) and total thyroxine (T4) kits obtained from Biomerica Inc. of USA were used for the analysis. TSH levels (1.80±1.62) in diabetics were significantly
lower (p=0.016) than the level in non-diabetic controls (2.34±1.24). Male diabetics had lower (p<0.05) levels of TSH (1.192±0.68
miu/ml) than diabetic females (1.90±1.70 mlu/mt). The level of T3 in diabetic males (125±97ng/ml) was higher than the level in females (98±75ng/dl). TSH (F=2.74, p=0.049), T4(F=56.87, p=0.001), T3(F=56.44, P=0.001) in diabetics and FT4 (F=5.74, p=0.002) in controls showed significant variation with the ages of the subjects. Out of 161 diabetics subjects studies
26.6% had low plasma thyroid hormone levels (FT4>2.01 ng/dl). This study has shown a high incidence (46.5%) of abnormal thyroid hormone levels among the diabetics in Nigeria
(hypothyroidism 26.6%, hyperthyroidism, 19.9%). The prevalence of hypothyroidism was higher in women (16.8%) than in men (9.9%),
while hyperthyroidism was higher in males (11%) than in females (8%). This study has defined thyroid function status of diabetics
in Calabar, Nigeria probably the first of such work in Africa. 相似文献
2.
Bhupinder Kaur Gill Promila Jindal Raj Kumar Shweta Tiwari Namrata Sharma Anupama Goel 《Indian journal of clinical biochemistry : IJCB》2007,22(1):148-151
Study was undertaken to assess thyroid status in hyperemesis gravidarum. 150 women pregnant with <20 weeks of gestation were
selected randomly and out of these 100 women presenting with hyperemesis formed study group while 50 normal pregnant women
served as controls. 53% of hyperemetic pregnant women were primigravidae and 82% of pregnant women presented with vomiting
at less than 12 weeks of gestation. Statistically significant, 22% of hyperemetic women had increased serum T3 levels while T4 levels were increased in 67% of women in study group as compared to 8% and 16% respectively in control group. TSH levels
were decreased in 18% of hyperemetic women as compared to 8% in control group with decrease in mean TSH level statistically
significant. 22% of hyperemetic women had electrolyte disturbances and 7% were ketonuric. In clinically euthyroid women, biochemically
altered thyroid function can attribute to vomiting and its prolongation to second trimester 相似文献
3.
Sarika Arora Ranjna Chawla Devika Tayal Vinod K. Gupta Jagdeep S. Sohi V. Mallika 《Indian journal of clinical biochemistry : IJCB》2009,24(4):370-374
Thyroid hormones regulate the renal hemodynamics and basal metabolic rate of most cells. This hospital-based case-control
study was done to evaluate the changes in biochemical markers of liver and kidney function in hypothyroid subjects before
and after treatment. The study included 176 subjects randomly selected from Thyroid clinics. Serum T3, T4, TSH, Liver and Kidney Function tests were analysed using standard kits. Forty-six hypothyroid patients were re-evaluated
6 weeks after thyroxine substitution therapy. Hypothyroid subjects (n=80) showed significantly raised serum creatinine and
uric acid levels as compared to euthyroid subjects (n=96). After 6 weeks of thyroxine replacement, serum creatinine and uric
acid decreased significantly and were comparable to euthyroid group. A positive correlation of ALT, AST, uric acid, protein
and albumin with TSH levels (p<0.05) and negative correlation of serum T4 levels with ALT, AST, proteins (p<0.05) was observed in the hypothyroid group. Hypothyroidism results in reversible impairment
of hepatorenal function. 相似文献
4.
Santosh C. Das Uche P. Isichei Aminu Z. Mohammed Abraham A. Otokwula Abiodun Emokpae 《Indian journal of clinical biochemistry : IJCB》2005,20(2):35-42
The effect of iodine deficiency (ID) on thyroid function in African women during pregnancy and postnatal period was examined,
for which very limited information is available. Serum T4, T3, TSH, TBG, thyroxine binding capacity (TBK), free thyroxine index (FT4I=T4/TBK) and T4/TBG were determined by ELISA technique in 32 pregnant women (resident of Plateau state, Nigeria- an ID zone) through pregnancy
upto term delivery, and in 5 women up to 6 weeks post delivery. Urinary iodide level was measured at delivery to determine
the degree of iodine deficiency. Results were matched with a non pregnant control comprising 44 subjects. 5555 birth weights
(BW) of term babies in the region were analysed to determine the prevalence of ‘small for dates’ (SFD) babies in the population.
Results show that the level of serum FT4I was elevated very significantly at late gestation (P<.001) but the women were not hyperthyroid, suggesting a marked disturbance
in binding of T4 with TBG during pregnancy. Five women with SFD babies were found in ‘compensated hypothyroid state’ and showed a significant
depression (p<.01) in serum T4/TBG, T4, FT4I and T3 levels to a low normal range, with a concurrent significant rise (p.<.001) in TSH level (above normal range) throughout pregnancy.
Incidence of SFD babies was higher (p.<.001) in ID zone (Bassa 15.2%) compared to control zone (Jos 9.8%). It is concluded
that a state of maternal ‘subclinical (compensated) hypothyroidism’ during pregnancy possibly plays an important role in the
aetiopathogenesis of SFD babies in Africans. A normal reference range for thyroid parameters at various stages of pregnancy
in healthy African women is established for the first time. 相似文献
5.
D. H. Shah Ajay Kumar M. G. R. Rajan U. R. Thakare S. M. Sharma 《Indian journal of clinical biochemistry : IJCB》1989,4(1):14-22
An external quality assessment was conducted for RIA of thyroid related hormones. Thirtyfive laboratories (35 for T4, 34 for T3 and 23 for TSH) from different parts of country participated in the programme. Twentyfour samples (16 pools: 5 simple and 11 manipulated pools) in 8 batches, 3 per batch per month were sent for analysis of T4, T3 and TSH. Some of the samples were repeated 3 times at different occasions to assess the imprecision of the laboratory. The overall mean percent CV obtained for T4, T3 and TSH were 22.7, 36.32 and 52.38 respectively. The recovery for added T4 was 86.73% while that for T3 was 117.4%. A large variation was obtained for recovery of TSH. For T4 estimations, 13 laboratories had a desirable performance i. e. bias less than ±10.0% and variability of bias (VB) and imprecision (IP) less than 15.0%. None of the laboratories had a desirable performance for T3 or TSH. The number of laboratories with acceptable performance i. e. bias between ±10.0–15.0%, VB and IP between 20.0–25.0% for T4, T3 and TSH were 4, 3 and 0 respectively. The number of laboratories which required attention (bias between ±15.0–20.0%; VB and IP between 20.0–25.0%) were 5,7 and 1 respectively. The unacceptable results with larger bias, VB and IP for T4, T3 and TSH were 6, 18 and 17 respectively. Our results are in general agreement that the performance of T4 assay is better than T3 and both in turn are much better than TSH. Quantitation of circulating thyroid hormones (TH) viz. tri-iodothyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH), which form the largest percentage of hormones estimated in a clinical laboratory is mainly done by radio-immunoassay (RIA) procedures. The reliability and reproducibility of these assays are generally monitored by using internal quality control (IQC) samples in every assay batch. Thus, the IQC provides information whether the assay results are satisfactory and can be released. However, external quality assessment (EQA) is a procedure whereby an external agency undertakes evaluation of the quality of an analytical service by providing samples for analysis to individual laboratories performing the assay. The data gathered is analysed collectively. EQA therefore provides a means by which performance of a laboratory is assessed in relation to other laboratories and matching the assay unbiased by removing systematic error, if present. This is important since RIA procedure involves several steps (collection and storage of samples, quality of the reagents, procedure followed for performance of an assay, counting equipment used and the mode of the data analysis) and therefore prone to systematic errors. We therefore undertook the EQA programme for assessment of thyroid related hormones as a joint collaborative project of Bhabha Atomic Research Centre, India and International Atomic Energy Agency. 相似文献
6.
Nosratollah Zarghami Mohammad Rohbani-Noubar Ali Khosrowbeygi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):182-185
Pregnancy is associated with significant, but reversible changes in thyroid function studies, which are among the most profound
seen as a result of a normal physiologic state. The present study was carried out to find out alterations in thyroid function
tests in each trimester in normal pregnant women as compared to non-pregnant women in Tabriz-Iran. A case-control study designed
with 229 normal pregnant women that randomly selected from the first (64 samples), the second (92 samples), and the third
(73 samples) trimesters and 250 randomly selected non-pregnant healthy female controls. Age range in both groups was 16–40
years. Thyroid function tests carried out by measuring serum levels of thyroid stimulating hormone (TSH), free and total thyroxin
(FT4, TT4), and free and total triiodothyronine (FT3, TT3) by commercially available radio immunoassay kits. We found that mean TT4 increased progressively during pregnancy. Our study showed increasing in serum levels of TT3 in the second trimester and then declining during the third trimester compared with non-pregnant women. We showed that FT4 strongly decreased during the third trimester. Free T3 showed declining in the second and third trimesters. Mean TSH did not show significant difference in each trimester compared
with non-pregnant women. The thyroid function tests in pregnancy should be interpreted against gestational age-related reference
intervals to avoid mis-interpretation of thyroid function during pregnancy. 相似文献
7.
Mala Mahto Baidarbhi Chakraborthy Srinivas H. Gowda Harneet Kaur Gaurav Vishnoi Pramod Lali 《Indian journal of clinical biochemistry : IJCB》2012,27(3):284-289
The implications of subclinical hypothyroidism (SCH) are many amongst which the most important is progression to overt hypothyroidism. Other debatable aspects are its association with cardiovascular risk, neuromuscular and psychiatric dysfunction, increased predisposition to developing metabolic syndrome and an underlying pro-inflammatory state. We aimed to study the lipid profile, lipoprotein(a) [Lp(a)] and hsCRP levels and insulin resistance in a group of patients with SCH in a referral hospital and see if any significant differences exist between them and euthyroids. This is a case–control study where the selection of controls and cases was based on the thyroid profile. Subjects were selected on their visit to clinical biochemistry lab for thyroid function tests. 33 euthyroids were taken as controls (Group I) and 38 patients comprising of subclinical hypothyroids were grouped as cases (Group II). Serum thyroid stimulating hormone (TSH) was in the range of 0.5–5.0 mIU/L for euthyroids and for subclinical hypothyroids the concentration of TSH was more than 5 mIU/L. The concentration of tri-iodothyronine (T3) and thyroxine (T4) were in normal reference range in both the groups. Individual lipid profile parameters failed to show a significant p value between cases and controls. The LDL/HDL ratio was highly significant (p value < 0.0001) and hsCRP was also statistically between the two groups (p value = 0.0054). Lp(a) and insulin resistance did not differ significantly. SCH is a common disorder that frequently progresses to overt hypothyroidism. This study underlines the importance of LDL/HDL ratio rather than measurement of individual lipid profile parameters in bringing to light the dyslipidemic state associated with SCH. Moreover the use of hsCRP to detect an underlying pro-inflammatory state in SCH can also be emphasized. 相似文献
8.
Ajay Kumar D. H. Shah M. G. R. Rajan U. R. Thakare S. M. Sharma 《Indian journal of clinical biochemistry : IJCB》1992,7(2):159-164
An external quality assessment was conducted to assess the performance of various laboratories for RIA of thhyroid related
hormones in two phases. In the first phase thirty five laboratories participated. At the end of first phase a meeting cum
workshop was organised to discuss the results of first phase, difficultires faced by the participants and pinpoint the short
oming. A second phase was then initiated with an objective of improvement in the performance, if any, where twelve samples
from four pools were distributed to twenty four laboratories who participated for the second phase. The overall return of
the results increased significantly from 71.8% (1586/2208) for the first phase to 92.4% (732/792) for the second phase. The
inter laboratory %CV for T3, T4 and TSH were lower during the second phase (30.6%, 19.0% and 31.6% respectively) as compared to those observed during first
phase (36.3%, 22.7% and 52.8% respectively). Similarly, there was an improvement in reproducibility of ALTM as %CV for T3, T4 and TSH decreased from 6.0%, 9.8% and 13.4% respectively to 4.5%, 4.6% and 8.5% respectively. The individual performances
of the participating laboratories viz. bias, variability of bias and imprecision also showed a trend towards improvement as
percent laboratories having desirable or acceptable results for T3, T4 and TSH increased from 10.7%, 60.7% and 0.0% respectively to 20.8%, 66.7% and 22.2% respectively. External quality assessment
thus appears to be beneficial in assessing the performane of a laboratory in comfparison with other laboratories and indeed
helps in improving the performance. 相似文献
9.
Shruti Mohanty W. Amruthlal G. C. Reddy G. Kusumanjali A. S. Kanagasabapathy Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2008,23(3):279-282
The aim of this study is to delineate laboratory diagnostic strategies for subclinical hypothyroidism in patients who are
clinically symptomatic but may have a normal thyroid profile. Tri — iodothyronine (T3), thyroxine (T4), thyroid stimulating hormone (TSH) and anti thyroid peroxidase antibodies (anti-TPO) were estimated on fasting blood samples
from 99 patients using electrochemiluminescence methods on ELECSYS 1010 (Roche). 74% of study subjects had elevated anti-TPO
levels.61% patients had subclinical hypothyroidism. 45 of the 61 subclinical hypothyroid patients had elevated anti-TPO levels
(73%). This is an important finding suggesting an autoimmune etiology for subclinical thyroid dysfunction with a higher risk
of developing overt hypothyroidism. 相似文献
10.
Graves’ disease (GD) is an organ-specific heterogenous autoimmune disorder associated with T-lymphocyte abnormality affecting
the thyroid, eyes and skin. GD is a multifactorial disease that develops as a result of complex interaction between genetic
susceptibility genes and environmental factors. It has been suggested that the Cytotoxic T lymphocytes associated molecule-4
(CTLA-4) is a genetic susceptibility candidate for GD. The present study was focused on A/G polymorphism at position 49 in
exon-1 of the CTLA-4 gene in 80 GD patients (GP) and 80 sex and age matched healthy individuals among South Indian (Madurai)
population. Serum concentrations of thyroid hormone (T4, T3 and TSH) were determined by using automated analyzer. The genomic DNA was isolated from the patient and control groups and
genotyping was performed using the polymerase chain reaction followed by restriction enzyme analysis using Bbv1. Significant difference (P < 0.001) was observed in the level of T3, T4 and TSH in GD patients and healthy individuals. The results revealed the CTLA-4 gene G/G genotype to be 32 (40%) in patients
and 26 (32.50%) in healthy individuals, A/G genotype to be 37 (46.25%) in patients and 25 (31.25%) in healthy individuals
and A/A genotype to be 11 (13.75%) in patients and 29 (36.25%) in healthy individuals. The calculated odds ratio (OR) in individuals
with mutant genotype (GG/AG) reveal 3.6 fold risk for GD (95% confidence interval = 1.6–7.8). The mutant “G” allele frequency
was observed to be 0.63 in GD patients and 0.48 in healthy individuals. Thus the present study demonstrates an association
between the CTLA-4 gene polymorphism and Graves’ disease. 相似文献
11.
12.
Pankaj Abrol Neeraj Sharma Harbans Lal 《Indian journal of clinical biochemistry : IJCB》1997,12(2):125-127
Serum vitamin E and cholesterol concentrations were measured in 50 children with protein energy malnutrition (PEM) and results
were compared with a group of 50 normal controls. Mean serum vitamin E concentration as well as vitamin E/cholesterol ratio
were found to be reduced in children with PEM while their mean serum cholesterol level was not significantly different from
the control group. Data suggest that the reduced levels of serum vitamin E in PEM may be a result of malnutrition per se. 相似文献
13.
Rebecca Abraham V. Srinivasa Murugan P. Pukazhvanthen S. K. Sen 《Indian journal of clinical biochemistry : IJCB》2009,24(1):52-59
Thyroid stimulating hormone (TSH), Free Thyroxine (FT4) and Free Triiodothyronine (FT3) were assayed in 505 women of this region. 60 women had previous history of thyroid disease. The remaining 445 women formed
the “Disease free group”. A “Reference group” was obtained by excluding women with previous and present history of thyroid
dysfunction. Of the total 505 women examined 15.8% had thyroid dysfunction and 84.2% were euthyroid. 11.5% were hypothyroid
(9.5% sub-clinical) and 1.8% hyperthyroid (1.2% clinical). The geometric mean TSH for the total population was 2.65 μIU/ml.
It was significantly (p=0.025) lower in the reference population 2.17 μIU/ml. There was no significant difference in the FT3 and FT4 values between groups. 19% of women over 60 years had elevated TSH above 4.5 μIU/ml. The 2.5 and 97.5 percentiles of the
reference population was 1.1–5.2 μIU/ml. 6.1% of women in the reference group had TSH levels above the reference intervals.
Hypothyroidism particularly sub-clinical hypothyroidism is predominantly present amongst women in this iodine sufficient region.
Evaluation of thyroid status could help in early detection and treatment. 相似文献
14.
Suparna Roy Ushasi Banerjee Anindya Dasgupta 《Indian journal of clinical biochemistry : IJCB》2016,31(1):43-49
In conjunction with thyroxine, bilirubin may play an important role for regulation of hsCRP level and a consequent pro-inflammatory condition in hypothyroidism. In present study we evaluated the dependence of hsCRP changes on total bilirubin (BT) and fT4 level in thirty overt (OH) and thirty subclinical hypothyroidism (SH). Serum BT, hsCRP, thyroxine and TSH were measured in both groups and compared with forty control subjects. Serum values of TSH, hsCRP showed raised (P < 0.001 for both) values with lower levels for fT4 and BT (P < 0.001 and 0.03 respectively) in hypothyroid patients compared to the controls. ANOVA showed significant increments in TSH and hsCRP values with decreases in fT4 among the control, SH and OH groups respectively (P < 0.001). BT values showed decrease in OH group only in comparison to controls (P = 0.04). Regression analysis revealed that hsCRP was negatively dependent on fT4 (β = −0.35, P = 0.002) and serum bilirubin (β = −0.40 and P < 0.001 respectively). Univariate general linear model analysis showed this dependence persisted even when carried out distinctly in SH and OH groups separately (P < 0.001). TSH did not show any significant predictive value on the hsCRP level in either of these two tests. From these analyses we suggest that serum hsCRP is closely integrated to a lowered synthesis of bilirubin and fT4 in hypothyroid patients. Furthermore, this causal relationship is not only limited to overt but also extends to the SH. 相似文献
15.
Activities of human hepatic drug metabolizing enzymes N-acetyl transferase (NATS) had earlier been recognized as a cause of inter-individual variation in the metabolism of drugs.
Therefore acetylation of many drugs in human exhibit genetic polymorphism. The aim of the study was to investigate if acetylator
status predispose diabetic mellitus patients more to the complications of renal disease, One hundred and twenty (120) diabetics
consisting of (50) Type 1 (T1) and 70 Type 2 (T2) diabetes mellitus patients and 100 healthy individuals as controls were classified as slow or rapid acetylator using sulphamethazine
(SMZ) as an in vivo probe. The percentage acetylation, recovery of SMZ, creatinine clearance and presence of urinary albumin
were determined. A significant difference (P < 0.05) was observed in the percentage of SMZ acetylated between slow and rapid acetylators in control, T1 and T2 subjects. The ratios of slow to rapid acetylators for T1, T2 and control subjects were 1:4, 3:2 and 2:3 respectively. No significant differences were observed in the percentage of SMZ
recovered in the urine of slow and rapid acetylators that are diabetics. The difference in creatinine clearance of slow and
rapid acetylators in T1 and T2 were significant (P < 0.05). 29% out of 120 (24.2%) diabetics (T1 and T2) exhibited albuminuria out of which 25 (86.2%) had slow acetylator status. These findings suggest that slow acetylator status
in diabetes mellitus could be a predisposing factor in the development of renal complications. This underscores the need for
a rapid pharmacogenetic testing and therapeutic drug monitoring in such patients. However this inference could be further
validated with a larger sample size. 相似文献
16.
Yogita S. Raut Uma S. Bhartiya Purushottam Kand Rohini W. Hawaldar Ramesh V. Asopa Lebana J. Joseph MGR Rajan 《Indian journal of clinical biochemistry : IJCB》2016,31(1):99-103
The aim was to study the genotoxic effect of high concentration of thyroxine (T4) in vivo in peripheral blood lymphocytes (PBL) of the patients suffering from thyroid disorders. The effect was compared by performing in vitro experiments with addition of increasing concentration of T4 (0.125–1 µM) in whole blood samples from healthy donors. Cytokinesis-blocked micronuclei (CBMN) assay method was used to assess the DNA damage in the PBL. The study included 104 patients which were grouped as control (n = 49), hyperthyroid (n = 31) and hypothyroid (n = 24). A significant increase in micronuclei (MN) frequency was observed in hyperthyroid patients when compared with the hypothyroid and euthyroid group thereby suggesting increased genotoxicity in hyperthyroidism (p < 0.001). A significant increase in MN frequency was observed at T4 concentration of 0.5 µM and above when compared to lower T4 concentrations (0.125 and 0.25 µM) and basal in in vitro experiments (p = 0.000). The results indicate that the T4 in normal concentration does not exhibit the genotoxic effect, as observed in both the in vivo and in vitro experiments. The toxicity of T4 increases at and above 0.5 μM concentration in vitro. Therefore acute T4 overdose should be handled promptly and effectively so as to avoid the possible genotoxic effect of high concentration of T4 in vivo. 相似文献
17.
M. N. Sadananda Adiga Sunil Chandy Girija Ramaswamy L. Appaji B. S. Aruna Kumari Lakshmi Krishnamoorthy 《Indian journal of clinical biochemistry : IJCB》2009,24(3):257-261
Remethylation of homocysteine to methionine is dependent on an adequate supply of one or more of the B vitamins like folate,
vitamin B12 and vitamin B6. Plasma total homocysteine (tHcy) is also influenced by genetic factors such as polymorphisms in the methylenetetrahydrofolate
reductase (MTHFR) gene. MTHFR is a flavo enzyme and a key player in folate metabolism and changes in its activity could modify
the susceptibility to Acute Lymphoblastic Leukemia (ALL). In this case — control study we have examined the effect of riboflavin
status as measured by erythrocyte glutathione reductase activation coefficient (EGRAC) on homocysteine levels along with vitamin
B12 and folate in pediatric ALL. Folate and B12 levels were significantly lower among cases as compared to controls while EGRAC and tHcy did not differ significantly among
the groups. The multivariate regression analysis revealed that in the ALL group EGRAC significantly influences tHcy levels
suggesting that riboflavin availability may be a predictor of tHcy levels in patients with ALL. This finding may have implications
for tHcy lowering therapy. 相似文献
18.
Emad F. Eskander Ahmed A. Abd-Rabou Hanaa H. Ahmed 《Indian journal of clinical biochemistry : IJCB》2013,28(4):413-417
Hormones play an important role in the digestive system. The main hormones that control digestion are gastrin, secretin, and cholecystokinin. Herein, the current study is concerned with assessing the effect of spasmo canulase and librax drugs on the human hormones profile. Blood samples were withdrawn from adult patients to measure serum FSH, E2, LH, prolactin, progesterone, DHEAS, testosterone, TSH, T3, T4, fasting insulin, and cortisol. All hormone concentrations were determined quantitatively using ELISA procedure. Intriguingly, the present study showed putative changes including thyroid and sex hormonal profiles. Eventually, we concluded that the prospective study could be important in drug dose optimization and providing new medical guidelines to avoid side effects that could harm patients. 相似文献
19.
Pannuru Padmavathi Vaddi Damodara Reddy Kodidela Swarnalatha Reddyvari Hymavathi N. Ch. Varadacharyulu 《Indian journal of clinical biochemistry : IJCB》2015,30(2):204-209
The present study was designed to understand the cigarette smoking-induced alterations in hormones and the resulting changes in platelet serotonin (5-hydroxytryptamine, 5-HT) and monoamine oxidase (MAO-B) activity in chronic smokers. Human male volunteers aged 35 ± 8 years, were divided into two groups, namely controls and smokers (12 ± 2 cigarettes per day for 7–10 years). Results showed that cigarette smoking significantly (p < 0.05) elevated plasma triiodothyronine (T3), cortisol and testosterone levels with significant (p < 0.05) reduction in plasma tryptophan and thyroxin (T4). Moreover, smokers showed reduced platelet 5-HT levels and MAO-B activity. In smokers, plasma cortisol was negatively correlated with tryptophan (r = −0.386), platelet MAO-B (r = −0.264), and 5-HT (r = −0.671), and positively correlated with testosterone (r = 0.428). However, testosterone was negatively correlated with platelet MAO-B (r = −0.315), and 5-HT (r = −.419) in smokers. Further, smokers plasma T3 levels were negatively correlated with platelet MAO-B (r = −0.398), and 5-HT (r = −0.541), whereas T4 levels were positively correlated with platelet MAO-B (r = 0.369), and 5-HT (r = 0.454). In conclusion, our study showed that altered testosterone and cortisol levels may aggravate behavior, mood disturbances and symptoms of depression by decreasing platelet 5-HT and MAO-B activity in smokers. 相似文献
20.
Hiren R. Modi Surendra S. Katyare Samir P. Patel 《Indian journal of clinical biochemistry : IJCB》2008,23(3):272-278
Effects of thyroidectomy (Tx) and subsequent treatment with 3,5,3’-triiodothyronine (T3), and combined treatment (TR) with T3 + thyroxine (T4) on substrate kinetics properties of cytochrome oxidase of rat liver mitochondria were examined. Tx resulted in lowering of cytochromes content with decrease in the enzyme activity, and Km and Vmax. T3 and TR regimens restored the cytochromes contents and the Vmax values to normal. In control, T3 and TR groups the enzyme activity resolved in two kinetic components; in Tx group three kinetic components were evident. The Km values for all components decreased significantly in the experimental groups with concomitant increase in catalytic efficiency,
Kcat/Km. Significant alterations in the contents of total phospholipid and of cholesterol were noted while the changes in the phospholipids
composition were only of restricted nature. Regression analysis revealed that total phospholipid, cholesterol and phosphatidylcholine,
phosphatidylethanolamine play significant role in fine tuning the enzyme activity. 相似文献