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1.
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice bfeeding program for the improvement of bacterial blight resistance.  相似文献   

2.
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance.  相似文献   

3.
Novel bacterial blight (BB) resistance gene(s) for rice was (were) introduced into a cultivated japonica rice variety Oryza sativa (cv. 8411), via somatic hybridization using the wild rice Oryza meyeriana as the donor of the resistance gene(s). Twenty-nine progenies of somatically hybridized plants were obtained. Seven somatically hybridized plants and their parents were used for AFLP (amplified fragment length polymorphism) analysis using 8 primer pairs. Results confirmed that these plants were somatic hybrids containing the characteristic bands of both parents. The morphology of the regenerated rice showed characters of both O. sativa and O. meyeriana. Two somatic hybrids showed highest BB resistance and the other 8 plants showed moderate resistance. The new germplasms with highest resistance have been used in the rice breeding program for the improvement of bacterial blight resistance. Project (No. 98001) supported by Ningbo Agriculture Key Scientific Research Foundation, China  相似文献   

4.
玉米单倍体的细胞学研究   总被引:2,自引:0,他引:2  
用细胞学方法对10株玉米单倍体的体细胞和性细胞进行染色体行为观察和计数,并对体细胞和性细胞进行了比较.结果表明:玉米单倍体的体细胞和性细胞中均有一定比例的二倍体细胞,体细胞中出现二倍体细胞的百分率平均为0.46%;在花粉母细胞中二倍体细胞的百分率比在体细胞中大大提高了,增加幅度最大的达到了12.86%.体细胞中存在二倍体细胞是性细胞中出现二倍体细胞的前提.  相似文献   

5.
在液体培养基中诱导产生的苜蓿体细胞胚,诱导第12天时生活力较高。超过12天后,体细胞胚由绿色向褐色转变,体细胞胚肥大肿胀,最后死亡。若将誘导12天产生的体细胞胚转移到成分相同的固体培养基上继续培养,则体胚进一步发育和转化成小苗。在固体培养基中加入脱落酸(ABA),可以抑制体细胞胚萌发,促进体细胞胚健康度提高,获得高质量的成熟休细胞胚。在固体培养基诱导产生的体细胞胚健康度比液体培养基中誘导的高得多。  相似文献   

6.
综述了观赏植物体细胞胚发生和植株再生的研究进展,包括体细胞胚的发生途径、影响因素和再生植株的遗传稳定性等,并探讨了观赏植物体细胞胚发生及植株再生的研究现状和应用前景.  相似文献   

7.
8.
体细胞胚发生是指体细胞在特定条件下未经性细胞融合而发育成胚状体的形态发生过程。药用植物体细胞胚发生的来源有 5条途径 ,其中以愈伤组织为最常见。近 2 0多年来研究者已通过体细胞胚发生获得了多种药用植物的再生植株 ,目前已初步进入商品化阶段  相似文献   

9.
A new asymmetric watermarking scheme is proposed in this letter. In the proposed scheme, a secret real fractional DCT-I transform and a primitive watermark are employed to generate an asymmetric watermark. The secret watermark for embedding is derived from the primitive watermark, and is embedded in the large fractional DCT-I transformation coefficients of a cover signal. The asymmetric detection procedure is performed using a correlation test. Simulation results showed that the asymmetric detection is reliable, and that the scheme can provide minimum security.  相似文献   

10.
植物体细胞胚胎发生影响因素研究进展   总被引:1,自引:0,他引:1  
论述了内源激素、培养基、外源激素、外植体类型、温度、光照等内外因素对植物体细胞胚胎发生的影响,并展望了植物体细胞胚胎发生的研究前景。  相似文献   

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