共查询到19条相似文献，搜索用时 171 毫秒
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用成年雄性Wistar大鼠,经4％多聚甲醛灌注固定,取下丘脑组织,行再固定24h,常规石蜡包埋和切片,用地高辛标记的cRNA探针进行厚位杂交,结果显示在弓状核及其邻近区域见到阳性神经元,阳性信号为蓝色颗粒,胞质、核膜及核仁呈阳性 阴性对照均无阳性反应出现 本实验证实,用石蜡切片进行原位杂交组织化学研究,能检测下丘脑生长抑素神经元基因表达产物mRNA,方法稳定,重复性好,便于普通实验室开展工作。 相似文献
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通过对38例银屑病（Psoriasis）皮肤组织中HB—EGF的表达,比较了核酸原位杂交过程中包括组织固定、脱水、浸蜡、包埋及切片过程中,外源性RNase控制与原位杂交结果之间的关系。结果表明,外源性RNase控制组的阳性信号高于非控制组,其原位杂交结果反映了基因表达的水平。提示在石蜡切片核酸原位杂交实验中,切片制备及保存过程中防止外源性RNA酶污染,是提高结果可信度的关键。 相似文献
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目的:探讨脑挫裂伤后c-jun和c-fos基因表达的特点.方法:利用自由落体法复制大鼠脑挫裂伤动物模型.伤后30′、1h、2h、4h、8h、24h和72h杀死大鼠取脑组织,石蜡切片,用PV6000免疫组织化学染色.结果:正常对照组假手术组c-fos无阳性表达,而c-jun呈弱阳性.模型组c-fos阳性神经元分布在伤灶周围区皮质1-2mm范围和部分海马;c-jun阳性神经元在伤侧皮质和海马广泛分布.结论:c-jun基因表达较c-fos更强烈、更广泛和表达时间更长. 相似文献
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目的:探讨脑挫裂伤后c-jun和c-fos基因表达的特点.方法:利用自由落体法复制大鼠脑挫裂伤动物模型.伤后30′、1h、2h、4h、8h、24h和72h杀死大鼠取脑组织,石蜡切片,用PV6000免疫组织化学染色.结果:正常对照组假手术组c-fos无阳性表达,而c-jun呈弱阳性.模型组c-fos阳性神经元分布在伤灶周围区皮质1-2mm范围和部分海马;c-jun阳性神经元在伤侧皮质和海马广泛分布.结论:c-jun基因表达较c-fos更强烈、更广泛和表达时间更长. 相似文献
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对绿竹笋组织块进行取材、固定、浸蜡、脱水、包埋、切片、染色等一系列过程的研究，掌握了绿竹笋石蜡切片制作方法，为进一步从解剖学上观察和研究绿竹笋老化机制提供了基础． 相似文献
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于惠敏 《山东教育学院学报》1997,(6)
1 前言在进行植物组织切片实验中,笔者对传统的石蜡切片方法做了几方面的改进。其中有两点比较重要:(1)固定剂不再含醛类、有毒金属离子或冰醋酸;(2)切片在脱蜡以前用爱尔新蓝(Alcian BuLe)和苏木精铝(Aluminum Hematoxylin)先后染色,获得了理想的效果。另外在其他一些方面也做了适当改进。这些改进方法的优点是:安全、节约、效率高、效果好。 相似文献
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王皓翔 《宁德师专学报(自然科学版)》2010,22(2):183-185
对尖叶木犀榄叶片进行取材、固定、染色、脱水、透明、浸蜡、包埋、切片、展片、脱蜡、封片等一系列过程的研究,掌握了尖叶木犀榄石蜡切片制作方法,为进一步从解剖学上观察和研究尖叶木犀榄的叶片细胞结构和组成提供了基础. 相似文献
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在组织切片上进行原位RT-PCR扩增(in situ RT-PCR),并在扩增过程中掺入地高辛标记的尿嘧啶核苷酸(Dig-UTP),观察新基因SNC 66在胃癌组织与正常胃粘膜中的表达差异,以分析SNC 66与胃癌发生的相关性。结果发现,25个循环时,20例胃癌标本中有4例阴性不表达,16例呈强阳性表达;当循环数降至10时,则6例呈阴性,9例呈弱阳性表达,另有5例呈强阳性表达。表明SNC 66基因在胃癌组织中存在明显的表达降低或表达缺陷,SNC 66可能是一个消化道肿瘤的负相关基因。 相似文献
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通过石蜡切片,PA S染色,在光学显微镜下系统观察了川白芷胚胎发育过程中淀粉的动态变化。结果表明:在胚囊发育过程中,淀粉积累逐渐增多,且胚囊边缘淀粉量的增加明显快于中部;合点珠心细胞中淀粉不明显,合点端淀粉少于珠孔端;胚囊细胞中淀粉具有由中央向边缘积累的趋势,细胞核因淀粉粒的挤压而偏于细胞边缘。 相似文献
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MENG Pan-qing HOU Gang ZHOU Gui-ying PENG Jia-ping DONG Qi ZHENG Shu 《Journal of Zhejiang University. Science. B》2005,6(9)
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method's relatively cheap and simple ZM- 1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology. 相似文献
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Meng PQ Hou G Zhou GY Peng JP Dong Q Zheng S 《Journal of Zhejiang University. Science. B》2005,6(9):853-858
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features that make TMA （tissue microarray） paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer, biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need any precision location system, and so is much cheaper than the currently used instrument. Our method＇s relatively cheap and simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology. 相似文献
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Meng Pan-qing Hou Gang Zhou Gui-ying Peng Jia-ping Dong Qi Zheng Shu 《浙江大学学报(A卷英文版)》2005,6(9):853-858
The ZM-1 tissue microarrayer designed by our groups is manufactured in stainless steel and brass and contains many features
that make TMA (tissue microarray) paraffin blocks construction faster and more convenient. By means of ZM-1 tissue microarrayer,
biopsy needles are used to punch the donor tissue specimens respectively. All the needles with the punched specimen cylinders
are arrayed into the array-board, with an array of small holes dug to fit the needles. All the specimen cylinders arraying
and the TMA paraffin block shaping are finished in only one step so that the specimen cylinders and the paraffin of the TMA
block can very easily be incorporated and the recipient paraffin blocks need not be made in advance, and the paraffin used
is the same as that for conventional pathology purpose. ZM-1 tissue microarrayer is easy to be manufactured, does not need
any precision location system, and so is much cheaper than the currently used instrument. Our method's relatively cheap and
simple ZM-1 tissue microarrayer technique of constructing TMA paraffin block may facilitate popularization of the TMA technology.
Project (No. G1998051200) supported by the National Basic Research Program (973) of China 相似文献
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许杰 《孝感职业技术学院学报》2011,(3):106-108
目的：探讨有效肠减压的有效性和生长抑素在有效肠减压中的使用时限;方法：选取2009年4月至2010年12月收治的肠梗阻病例46例,在常规治疗的基础上,使用生长抑素和营养支持的有效的肠减压方法,比较患者治疗前后腹痛腹胀症状改善情况,手术中转率,术中所见肠管水肿情况及治疗前、使用生长抑素后的血象、生化指标、X—ray变化情况。结果：使用生长抑素加肠外营养支持的有效肠减压明显改善临床症状,总的有效率高,可明显降低手术探查率,使用有效肠减压的平均有效时间约为5．3天。 相似文献
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目的探讨肺和食道鳞状细胞癌中P27基因蛋白的表达状况.方法收集手术切除鳞癌标本63例,10%中性甲醛固定,石蜡切片,厚度5μm.超敏型S-P免疫组织化学染色.结果P27在鳞状细胞癌中总阳性率39.68%,在肺和食道中阳性率分别为36.00%和42.15%.在高、中分化组阳性率为36.00%,低分化组阳性率为53.85%.结论鳞癌组织中P27阳性表达与分化程度及是否转移的相关性有深入研究的必要. 相似文献
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石蜡催化氧化改性工艺 总被引：11，自引：0，他引：11
罗力 《荆门职业技术学院学报》2004,19(3):8-10,22
对石蜡催化氧化改性工艺进行了研究，考察了反应温度、空气流量、催化剂加入量和反应时间对石蜡氧化过程的影响．在适当的反应务件下，可以得到不同性能要求的氧化石蜡产品． 相似文献