共查询到20条相似文献，搜索用时 31 毫秒
1.
Huang He-feng Xia Ya-xian Shi Yi-fu Zhou Fu-zhen Yeung Shu-biao Ho Pak-chung 《浙江大学学报(A卷英文版)》2000,1(3):342-346
The present study was undertaken to evaluate the endometrial secretion and synthesis of proteins in hamsters that were subjected
to ovarian stimulation with or without GnRH agonist (Leuprolide Acetate, LA). The results of protein resolution revealed that
the molecular weight of the proteins increased from 31D to 66D, while the intensities of photographed protein bands in the
hamsters treated with LA and pregnant mare’ s serum gonadotropin (PMSG) were less than that in those treated with PMSG alone.
The amounts of low and medium weights (31kD and 45kD) proteins newly synthesized in LA+PMSG group and saline group (control)
were significantly higher than that in PMSG group. The synthesized and secreted proteins in groups LA and control were similar.
The data suggested that a physiologic endometrial protein secretory and synthetic change with GnRH agonist regarcded as a
possible cause of the higher pregnancy rate in in vitro fertilization. The degree of endometrial secretion and synthesis varied
considerably with the type of ovarian stimulation used.
Project supported by China Medical Board of New York Inc., USA 相似文献
2.
Qiong-yan Lin Yi-feng Wang Hui-nan Weng Xiu-jie Sheng Qing-ping Jiang Zhi-ying Yang 《Journal of Zhejiang University. Science. B》2012,13(11):894-903
Background and objective: Gonadotropin-releasing hormone (GnRH) plays an important role in the regulation of ovarian function and ovarian cancer cell growth. In this study, we determined whether administration of the GnRH agonist (GnRHa), triporelin, prior to cisplatin treatment affects cisplatin and/or prevents cisplatin-induced ovarian damage. Methods: nu/nu mice were injected with ovarian cancer OVCAR-3 cells intraperitoneally. After two weeks, the mice were treated with saline (control), cisplatin, GnRHa, or cisplatin plus GnRHa for four weeks. At the end of the experimental protocol, blood, tumor, ovary, and uterine tissues were resected for hematoxylin and eosin (H&E) staining, immunohistochemical analyses of Ki67, nuclear factor-κB (NF-κB), and caspase-3, transmission electron microscopy of apoptosis, or enzyme-linked immunosorbent assay (ELISA) analyses of anti-Mullerian hormone (AMH). Results: Cisplatin treatment effectively inhibited tumor growth in mice treated with human ovarian cancer cells; however the treatment also induced considerable toxicity. Immunohistochemical analyses showed that Ki67 expression was reduced in cisplatin-treated mice compared to control (P<0.05), but there was no statistically significant differences between cisplatin-treated mice and cisplatin plus GnRHa-treated mice (P>0.05), while expressions of NF-κB and caspase-3 were reduced and induced, respectively, in cisplatin-treated mice and cisplatin plus GnRHa-treated mice. Apoptosis occurred in the GnRHa, cisplatin, and cisplatin plus GnRHa-treated mice, but not in control mice. Ovaries exposed to GnRHa in both GnRHa mice and cisplatin-treated mice (combination group) had significantly more primordial and growth follicles and serum levels of AMH than those in the control mice and cisplatin-treated mice (P<0.05). Conclusions: Administration of GnRHa to mice significantly decreased the extent of ovarian damage induced by cisplatin, but did not affect the anti-tumor activity of cisplatin. 相似文献
3.
哺乳动物排卵的过程包括：脑下垂体前叶分泌LH到体循环后,下丘脑脉冲性释放GnRH到垂体门脉系统．而LH峰介导了卵泡成熟、卵泡破裂和排出。哺乳动物根据引起下丘脑分泌GnRH方式的不同分为自发性排卵（人、羊、牛、马和猪等）和诱发性排卵（兔、貂、猫和骆驼等）动物,这其中自发性排卵动物具有一定的发情周期。早期研究发现交配过程能促发羊驼和驼马的排卵．最新研究发现精液中存在一种促排卵因子（OIF）,这有助于了解自发性和诱发性排卵动物排卵的机制。OIF能刺激LH分泌、排卵和黄体腺的发育,并且通过体循环而非局部通路发挥作用。OIF是一种能耐热和抗蛋白酶K消化的蛋白质分子（分子团为14kDa）,它可能是一种蛋白复合体或者激素原的一部分。OIF发挥作用是和剂量相关的。OIF已被证实存在于骆驼、牛、马、猪和袋熊等动物中。OIF在自发性排卵动物中能介导初情期小鼠促排、能改变母牛卵泡渡的动态变化、能提升LH的释放（体外培养大鼠、小鼠、豚鼠、兔、驼马和牛的脑下垂体细胞）。 相似文献
4.
《天津大学学报(英文版)》2017,(5)
For the proteins that cannot be expressed exactly by cell expression technology(e.g., proteins with multiple posttranslational modifications or toxic proteins), chemical synthesis is an important substitute. Given the limited peptide length offered by solid-phase peptide synthesis invented by Professor Merrifield, peptide ligation plays a key role in long peptide or protein synthesis by ligating two small peptides to a long one. Moreover, high-molecularweight proteins must be synthesized using two or more peptide ligation steps, and sequential peptide ligation is such an efficient way. In this paper, we reviewed the development of chemical protein synthesis, including solid-phase peptide synthesis, chemical ligation, and sequential chemical ligation. 相似文献
5.
This study sought to examine distinctions in social competencies between children with learning disabilities (LD) and other children who also experience academic difficulties. Eighty-five children with LD (54 male, 31 female) in Grades 3 through 6 from a large urban school district were compared to a group of low achieving (LA) peers matched on achievement as well as sex, race, and grade. The samples were 42% black, 39% Hispanic, and 19% Anglo. Both groups completed two self-concept questionnaires, a loneliness scale, and a measure of their social relationships outside of school. In addition, their classmates completed a peer rating scale and their teachers completed two ratings of the child's social skills. The results indicated that children with LD and LA children were comparable on most measures, although children with LD reported themselves as being less lonely than LA children. In addition, regular-class children rated children with LD as more likable than LA children. The results highlight similarities in the social competencies of children with LD and LA children and suggest that special education classes may offer some social advantages to children with mild handicaps. 相似文献
6.
目的：探讨Tei指数原发性高血压患者心脏功能的临床应用价值。方法：原发性高血压患者176例．按Ganau分类法将原发性高血压分为4型；正常构型（A组，46例）、向心性重构（B组，53例）、向心性肥厚（C组，40例）、离心性肥厚（D组，37例）；正常对照组35例。以常规超声心动图检查测量舒张末期室间隔、左室后壁厚度及左室内径（IVS、LVPW、LVDed），二尖瓣血流频谱图舒张早期与舒张晚期血流峰值比（E／A），左室射血分数（EF）及Tei指数，进行对比分析。结果：1.一般资料的比较：各组同年龄、性别差异均无显著性意义，C、D组病程有显著性差异，C、D组收缩压最高（P〈0．05），B、C、D组脉压最高（P〈0．05）；2．各组超声心动图结构参数比较：C组、D组LA最大（P〈0．05）；B组LVDS最小（P〈0．05），D组LVDS最大（P〈0．05）；C组IVS、LVPW最厚（P〈0．05）；B组、D组IVS、LVPW轻度增厚（P〈0．05）；C组RWT明显厚于其它各组（P〈0．01）；C组、D组LVMI质量显著增加（P〈0.01），但C组、D组之间无统计学意义；3．各组超声心动图功能参数比较：高血压各组的E／A值均减低（P〈0．05），EF只有D组与对照组比较有显著性意义；高血压各组Tei指数均较对照组增加，呈递增趋势，各组间有显著性意义。结论：Tei指数可以客观、真实的评价高血压患者不同左室构型的心功能情况，对于评估疾病的治疗效果和预后具有很高的临床应用价值。 相似文献
7.
Hooded rats and golden hamsters were shocked by one of two prods in a chamber with a sawdust-covered floor. Rats buried the prod through which they had been shocked, but hamsters displayed no burying behavior. Hamsters may not have buried the prod because they could not perform the required motor pattern. However, hamsters can carry and pile food pellets. Therefore, in a second experiment, rats and hamsters were shocked in a chamber with wooden blocks on the floor. Rats piled blocks around the prod through which they had been shocked, but hamsters did not. The third experiment established that, like rats, hamsters can associate a prod with shock in one trial, since they showed differential avoidance of a prod through which they had been shocked. Since hamsters are nonsocial and rats are social, these results are consistent with suggestions that burying sources of aversive stimulation evolved as an altruistic behavior. 相似文献
8.
ＩＮＴＲＯＤＵＣＴＩＯＮＢｒｏａｄｂｅａｎｗｉｌｔｖｉｒｕｓ (ＢＢＷＶ)ｉｓｔｈｅｔｙｐｅｍｅｍｂｅｒｏｆｔｈｅｇｅｎｕｓＦａｂａｖｉｒｕｓ.Ｉｔｈａｓａｗｉｄｅｈｏｓｔｒａｎｇｅａｍｏｎｇｄｉｃｏｔｙｌｅｄｏｎｓａｎｄｓｏｍｅｆａｍｉｌｉｅｓｏｆｍｏｎｏｃｏｔｙｌｅｄｏｎｓ,ａｎｄｉｓａｎｅｃｏｎｏｍｉｃａｌｌｙｉｍ ｐｏｒｔａｎｔｖｉｒｕｓｉｎＣｈｉｎａ (Ｚｈｏｕｅｔａｌ.,1 994 ) .ＢＢＷＶｈａｓｉｓｏｍｅｔｒｉｃｐａｒｔｉｃｌｅｓ,ｈｅｘａｇｏｎａｌｉｎｏｕｔ ｌｉｎｅａｎｄ 3 0ｎｍｉｎｄｉａｍ… 相似文献
9.
采用还原胺化法将苏氨酸成功转变成了其氮二甲基衍生物,通过1H-NMR和13C-NMR证实了其结构,采用量子化学方法在DFT-B3LYP/6-31G*水平上计算了该化合物的反应热、电荷分布和前线轨道能级。 相似文献
10.
11.
A group of seventh- and eighth-grade Italian students with low achievement (LA) in learning English as a foreign language (FL) was selected and compared to a group with high achievement (HA) in FL learning. The two groups were matched for age and nonverbal intelligence. Two experiments were conducted to examine the participants' verbal and nonverbal learning skills, such as native language reading accuracy, speed and comprehension, calculation, and attention and self-regulation. Both experiments showed that the LA group seemed at risk for reading comprehension difficulties, but its reading speed and accuracy were within the average range according to Italian norms. The results also excluded the possibility that FL learning difficulties of LA participants could be associated with a deficit in calculation. Furthermore, according to teachers' ratings, children with LA appeared at risk for attention-deficit disorder (ADD). The pattern of learning difficulties of seventh- and eighth-grade participants with LA appeared to be not completely comparable with that of high school students at risk of FL learning difficulties as described in the literature. 相似文献
12.
腹腔镜阑尾切除术与传统阑尾切除术疗效对比 总被引：2，自引：0，他引：2
智慧 《河北北方学院学报(医学版)》2008,25(4)
目的:比较腹腔镜阑尾切除术(laparoscopic appendectomy,LA)与传统剖腹阑尾切除术(open appendectomy,OA)的临床疗效。方法:将156例阑尾炎病例随机分成两组,一组72例行LA,另一组84例行OA,比较两种术式的手术时间、平均住院费用、住院时间,切口长度、术后镇痛剂应用、术后6h开始下床活动、术后24h内肛门排气例数、放置引流管率、切口感染等指标。结果:LA组的术后24h内肛门排气时间、放置引流管率、切口感染、平均住院时间、切口长度、粘连性肠梗阻、镇痛剂应用例数、术后6h开始下床活动例数与OA组比较,差异均有统计学意义(P<0.05);而两组手术时间比较差异无统计学意义(P>0.05);LA组住院费用较OA组明显增高(P<0.05)。结论:LA与OA比较,有手术损伤小、疼痛轻、恢复快、住院时间短、并发症少等优点,可作为大多数阑尾炎的首选手术方式。 相似文献
13.
Sexually naive male hamsters were paired with ovariectomized females or with castrated males. On half of the trial days, the stimulus animals were scented with vaginal secretion from estrous females, and on half of the trials they were left unscented. Added vaginal secretion resulted in an increased frequency of mounting and decreased latency to mount, and an increase in the amount of time the males remained near the stimulus animals. These results demonstrate that the vaginal secretion has attractant and sexual excitant effects on naive males and thus is a source of sex pheromones. Presence of the secretion also resulted in a decrease in some measures of agonistic behavior, suggesting anti-aggressive functions as well.
相似文献14.
Endometrial stromal tumors(ESTs) include endometrial stromal nodule(ESN), low-grade endometrial stromal sarcoma(LG-ESS), high-grade endometrial stromal sarcoma(HG-ESS), and undifferentiated uterine sarcoma(UUS). Since these are rare tumor types, there is an unmet clinical need for the systematic therapy of advanced LG-ESS or HG-ESS. Cytogenetic and molecular advances in ESTs have shown that multiple recurrent gene fusions are present in a large proportion of LG-ESSs, and HG-ESSs are identified by the tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein epsilon(YWHAE)-family with sequence similarity 22(FAM22) fusion. Recently, a group of ESSs harboring both zinc finger CCCH domain-containing protein7 B(ZC3H7B)-B-cell lymphoma 6 corepressor(BCOR) fusion and internal tandem duplication(ITD) of the BCOR gene have been provisionally classified as HG-ESSs. In this review, we firstly describe current knowledge about the molecular characteristics of recurrent aberrant proteins and their roles in the tumorigenesis of LG-ESSs and HG-ESSs. Next, we summarize the possibly shared signal pathways in the tumorigenesis of LG-ESSs and HG-ESSs, and list potentially actionable targets. Finally, based on the above discussion, we propose a few promising therapeutic strategies for LG-ESSs and HG-ESSs with recurrent gene alterations. 相似文献
15.
目的研究乙醇对雄性大鼠下丘脑促性腺激素释放激素神经元（GnRH neurons）合成和释放激素功能的影响。方法健康Wistar雄性大鼠40只,随机分为4组：对照组、低浓度乙醇组、中浓度乙醇纽和高浓度乙醇组。在灌服不同浓度的乙醇后2h,采用酶法检测血中乙醇浓度（blood alcohol concentration,BAC）;免疫组织化学法观察下丘脑促性腺激素释放激素。（Gonadotropin-releasing hormone,GnRH）神经元密度和染色反应的变化。结果 与对照组相比,随着灌胃乙醇浓度的升高,各实验组大鼠BAC值逐渐升高,并且均已达到乙醇中毒的标准（500-1000mg／L）：高浓度乙醇组大鼠下丘脑内GnRH阳性神经元密度和染色强度较对照组明显减小（p〈0．01）,中、低浓度乙醇组下丘脑内GnRH阳性神经元密度和染色强度较对照组变化不显著（P〉0.05）。结论急性乙醇中毒雄性大鼠,下丘脑神经递质GnRH神经元合成和分泌功能受抑,这可能与乙醇对生殖行为的抑制有关。 相似文献
16.
An apparatus designed by Berlyne was used to dissociate locomotor activity and inspective exploratory responses with the aid of traditional manipulators. In this apparatus, novel and complex stimulation increased exploration but did not affect locomotor activity (LA), d-amphetamine (1.5 mg/kg) increased LA but decreased exploration. These findings provide a double dissociation of the behavioral components. In addition, low intrasubject correlations for the two behaviors were demonstrated. Results are discussed with reference to the need for simultaneous separate measures to obtain valid indices of exploratory behavior and LA. 相似文献
17.
18.
A Western blot-based investigation of the yeast secretory pathway designed for an intermediate-level undergraduate cell biology laboratory 
下载免费PDF全文
下载免费PDF全文 Hood-Degrenier JK 《CBE life sciences education》2008,7(1):107-117
The movement of newly synthesized proteins through the endomembrane system of eukaryotic cells, often referred to generally as the secretory pathway, is a topic covered in most intermediate-level undergraduate cell biology courses. An article previously published in this journal described a laboratory exercise in which yeast mutants defective in two distinct steps of protein secretion were differentiated using a genetic reporter designed specifically to identify defects in the first step of the pathway, the insertion of proteins into the endoplasmic reticulum (Vallen, 2002). We have developed two versions of a Western blotting assay that serves as a second way of distinguishing the two secretory mutants, which we pair with the genetic assay in a 3-wk laboratory module. A quiz administered before and after students participated in the lab activities revealed significant postlab gains in their understanding of the secretory pathway and experimental techniques used to study it. A second survey administered at the end of the lab module assessed student perceptions of the efficacy of the lab activities; the results of this survey indicated that the experiments were successful in meeting a set of educational goals defined by the instructor. 相似文献
19.
采用免疫组化SP法检测Livin和Fhit在30例正常子宫内膜和70例子宫内膜癌组织中的表达,研究凋亡抑制蛋白Livin和Fhit在子宫内膜癌中的表达和意义。结果显示Livin蛋白在正常子宫内膜组织中表达明显低于子宫内膜癌组织(P<0.05);Fhit蛋白在正常子宫内膜组织中表达明显高于子宫内膜癌组织(P<0.05);在子宫内膜癌组织中,随组织学分级和病理分期的增加,Livin蛋白表达阳性率逐渐上升,Fhit蛋白表达阳性率逐渐下降,而且Fhit蛋白表达与淋巴结转移相关(P<0.05);Livin蛋白和Fhit蛋白表达呈负相关(P<0.05)。结果提示Livin和Fhit异常表达可能与子宫内膜癌的发生、发展关系密切,联合检测Livin和Fhit有助于子宫内膜癌早期诊断、治疗及预后判断。 相似文献
20.
目的：探讨抑癌基因第10染色体同源丢失性磷酸酶一张力蛋白基因（PTEN）蛋白表达与子宫内膜癌的发生、发展的关系。方法：采用免疫组织化学S-P法检测PTEN蛋白在56例子宫内膜癌及30例正常子宫内膜组织的表达。结果：56例子宫内膜癌中PTEN蛋白表达缺失率为57．1％，明显高于正常子宫内膜中的完全表达（P〈0．05）。PTEN蛋白在低分化组的表达缺失率明显高于中高分化组（P〈0．05）。结论：抑癌基因PTEN蛋白表达缺失在子宫内膜癌的发生、发展、浸润等过程中有一定作用。 相似文献