排序方式： 共有17条查询结果，搜索用时 171 毫秒
11.
ＩＮＴＲＯＤＵＣＴＩＯＮＡｎｉｎｃｒｅａｓｉｎｇｄｅｍａｎｄｆｒｏｍｃｏｎｓｕｍｅｒｓｆｏｒｈｅａｌｔｈｙａｎｄｌｏｗｃｏｓｔｍｅｄｉｃａｌｓｈａｓｌｅｄｔｏｔｈｅｄｅ ｖｅｌｏｐｍｅｎｔｏｆａｌｔｅｒｎａｔｉｖｅｍｅｄｉｃａｌｔｈｅｒａｐｙａｇｅｎｔｓｕ 相似文献
12.
The production of elastase by Bacillus sp. EL31410 at various temperatures was investigated. In order to study the effect of temperature on elastase fermentation, different cultivation temperatures, ranging from 39 ℃ to 28 ℃, were evaluated in shake flask. The result indicated that 37 ℃ was best for cell growth at earlier stage; while maximum elastase activity was obtained when the cells were cultivated at 30 ℃. This result was verified by batch fermentation in 5-L bioreactor under 37 ℃ and 30 ℃ temperature, respectively. The specific cell growth rate at 37 ℃ was higher than that at 30 ℃ latory temperature cultivation mode were evaluated in the next study. When compared to single temperature of 37 ℃ or 30 ℃, both two-stage temperature shift strategy and oscillatory temperature strategy improved biomass but did not yield the same result as expected for elastase production. The maximum biomass (both 8.6 g/L) was achieved at 30 h at 37 ℃, but at 42 h using two-stage temperature cultivation strategy. The highest elastase production (652 U/ml) was observed at 30 ℃ in batch process. It was concluded that cultivation at constant temperature of 30 ℃ was appropriate for elastase production by Bacillus sp. EL31410. 相似文献
13.
A newly isolated strain EL31410, producing elastase (E.C3.4.4.7) with high elastolytic activity was identified as Bacillus sp. In the medium optimization, it was found that wheat bran and soybean flour hydrosate were the best crude carbon and nitrogen source for enzyme production, respectively. Addition of corn steep flour can affect the bacterium growth and elastase production. A fractional factorial design was applied to study the main factors that affect the enzyme production, and central composite experimental design and response surface methodology were adopted to derive a statistical model for the effect of wheat bran and soybean flour hydrosate on elastase production. The experimental results showed that wheat bran had positive effect but soybean flour hydrosate had negative effect, on enzyme production. An initial concentration of 3.4%(w/v) wheat bran and 9.4%(v/v) soybean flour hydrosate were found to be optimal for enzyme production in batch culture. The time course of elastase production in the optimized medium composition was also described. 相似文献
14.
15.
羽毛的生物降解及其酶学性质的研究 总被引：1，自引：0，他引：1
羽毛角蛋白是一种环境污染有机废弃物,通过微生物对角蛋的进行降解为合理利用,可以使它成为丰富的饲料蛋白来源。适当的降解方法对于羽毛资源的转化有着重要作用。采用正交设计优化地衣芽孢杆菌细胞转化羽毛的条件,得出角蛋白酶的合成和羽毛的分解指标,三因子最合适配比为:液料比20:1,反应pH 7.5,BC细胞加量0.5克,40℃下120 r/min处理72 h,其中细胞加量对羽毛分解有关键影响。粗酶液最适温度在37℃,在35℃~40℃有很好的热稳定性。不同pH下酶活变化明显,在pH 5-8范围内比较稳定。 相似文献
16.
17.
INTRODUCTION The hop plant (Humulus lupulus L., Cannabi- naceae) is a dioecious twining perennial widely cul- tivated throughout the temperate zones of the world. The inflorescences (hops or hop cones) are used in breweries to give beer its characteristics such as fla- vor and aroma. In this respect, the bitter acids and essential oils have received much attention. Carbon dioxide (CO2) is currently the most accepted solvent for the manufacture of hops extracts by breweries. Its extracts… 相似文献