Insulator-based dielectrophoresis of mitochondria     

Jinghui Luo  Bahige G. Abdallah  Gregory G. Wolken  Edgar A. Arriaga  Alexandra Ros 《Biomicrofluidics》2014,8(2)

Isolated mitochondria display a wide range of sizes plausibly resulting from the coexistence of subpopulations, some of which may be associated with disease or aging. Strategies to separate subpopulations are needed to study the importance of these organelles in cellular functions. Here, insulator-based dielectrophoresis (iDEP) was exploited to provide a new dimension of organelle separation. The dielectrophoretic properties of isolated Fischer 344 (F344) rat semimembranosus muscle mitochondria and C57BL/6 mouse hepatic mitochondria in low conductivity buffer (0.025–0.030 S/m) at physiological pH (7.2–7.4) were studied using polydimethylsiloxane (PDMS) microfluidic devices. First, direct current (DC) and alternating current (AC) of 0–50 kHz with potentials of 0–3000 V applied over a channel length of 1 cm were separately employed to generate inhomogeneous electric fields and establish that mitochondria exhibit negative DEP (nDEP). DEP trapping potential thresholds at 0–50 kHz were also determined to be weakly dependent on applied frequency and were generally above 200 V. Second, we demonstrated a separation scheme using DC potentials <100 V to perform the first size-based iDEP sorting of mitochondria. Samples of isolated mitochondria with heterogeneous sizes (150 nm–2 μm diameters) were successfully separated into sub-micron fractions, indicating the ability to isolate mitochondria into populations based on their size.  相似文献   

“The Enchanted Palace” at Kensington     

Alexandra Kim 《Curator: The Museum Journal》2011,54(4):403-412

Abstract Kensington Palace, London, has been home to kings and queens, dukes and princesses for 300 years. It was the focus for the tremendous public outpouring of grief following the death of Diana, Princess of Wales in 1997. Recently, the historic building was transformed into “The Enchanted Palace at Kensington,” which sent visitors on a quest through the sumptuous rooms to find seven of the palace’s princesses. On their journey, visitors discovered fantastical, fairy‐tale‐like installations, some of them by the best British fashion designers, and inspired by Kensington Palace’s incredible history.  相似文献   

How achievement emotions impact students' decisions for online learning,and what precedes those emotions     

Dirk T. Tempelaar  Alexandra Niculescu  Bart Rienties  Wim H. Gijselaers  Bas Giesbers 《The Internet and Higher Education》2012,15(3):161-169

This empirical study investigates students' learning choices for mathematics and statistics in a blended learning environment, composed of both online and face-to-face learning components. The students (N = 730) were university freshmen with a strong diversity in prior schooling and a wide range of proficiency in quantitative subjects. In this context, we investigated the impact that individual differences in achievement emotions (enjoyment, anxiety, boredom, hopelessness) had on students' learning choices, in terms of the intensity of using the online learning mode versus the face-to-face mode. Unlike the general level of learning activities, which is only minimally influenced by achievement emotions, these emotions appear to have a moderately strong effect on a student's preference for online learning. Following this, we explored the antecedents of achievement emotions. Through the use of path-modeling, we conclude that while goal setting behavior only marginally impacts achievement emotions, effort views—a crucial component of the social-cognitive model of implicit theories of intelligence—have a substantial impact on achievement emotions.  相似文献   

Rethinking our mobility: supporting our patrons where they live     

Gomes A  Abate L 《Medical reference services quarterly》2012,31(2):140-149

This article describes initiatives undertaken by one academic medical library to support patrons using handheld mobile devices. These initiatives included efforts to assist patrons with installation and use of mobile information resources, educate librarians regarding content and navigation of mobile apps and websites, and leverage tools to promote the library in a mobile environment.  相似文献   

明确自己的价值     

刘克 《英语沙龙》2010,(1)

不知道自己将来做什么?学校和家之间两点一线的生活让你感到厌倦?来自老师和家长的压力很想让你逃跑吗?不必那么急切。世界上不会有两片相同的树叶,不会有一摸一样的人,更不会有能担当相同任务的同样能力和价值的人。尽力展现你独特的一面吧,那就是你的价值。  相似文献   

Practice what you preach: Adoption of internal campus innovations at Dutch research-intensive universities     

Małgorzata Rymarzak  Alexandra den Heijer  Monique Arkesteijn  Mathilda Du Preez 《Higher Education Quarterly》2023,77(3):447-464

Campus decision makers are increasingly expected to adopt ‘campus innovations’ (affecting real estate and different facilities), not only from the market and demand-led (external campus innovations), but also developed by the university's own scientists (internal campus innovations). The adoption of the latter can be driven and hindered by many unique factors that campus decision makers have not dealt with before. To provide insight into them, qualitative data were collected from 13 out of 14 Dutch research-intensive universities. The results indicate that internal campus innovations are driven by co-creation stimulation, collaborative partnership, transparency and accountability, and local development contribution. Their adoption, however, may be obstructed by barriers embedded in the interaction between campus decision makers and scientists, organizational university context, funds unavailability and innovations' supply-pushed characteristics. An increased understanding of these barriers and the practices to overcome them is crucial for universities' campus decision makers to actively engage in the adoption of internal campus innovations.  相似文献   

Counselor educators’ views on cognitively complex trainees: A Q method study     

Alexandra Gantt-Howrey  Gülşah Kemer 《Counselor Education & Supervision》2023,62(4):297-310

Our Q method exploration of counselor educators’ perspectives of minimally acceptable cognitive complexity (CC) in trainees at the end of internship yielded two factors: Trainee's Conceptual Integration Ability and Trainee's Ability to Apply Integrated Knowledge. Implications for teaching, supervision, and research pertinent to assessment of and enhancement of CC conclude.  相似文献