Graduate and postgraduate state university education of laboratory specialists in Hungary: medical doctors, pharmacists and laboratory analysts     

Kovács GL  Ludány A  Koszegi T  Liszt F  Kellermayer M 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2011,21(1):22-29

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Pheromone synthesis in a biomicroreactor coated with anti-adsorption polyelectrolyte multilayer     

Dimov N  Muñoz L  Carot-Sans G  Verhoeven ML  Bula WP  Kocer G  Guerrero A  Gardeniers HJ 《Biomicrofluidics》2011,5(3):34102-3410212

To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His(6)-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).  相似文献   

The dynamics of the impact and coalescence of droplets on a solid surface   总被引：1，自引：0，他引：1  

Castrejón-Pita JR  Betton ES  Kubiak KJ  Wilson MC  Hutchings IM 《Biomicrofluidics》2011,5(1):14112

A simple experimental setup to study the impact and coalescence of deposited droplets is described. Droplet impact and coalescence have been investigated by high-speed particle image velocimetry. Velocity fields near the liquid-substrate interface have been observed for the impact and coalescence of 2.4 mm diameter droplets of glycerol∕water striking a flat transparent substrate in air. The experimental arrangement images the internal flow in the droplets from below the substrate with a high-speed camera and continuous laser illumination. Experimental results are in the form of digital images that are processed by particle image velocimetry and image processing algorithms to obtain velocity fields, droplet geometries, and contact line positions. Experimental results are compared with numerical simulations by the lattice Boltzmann method.  相似文献   

Adolescents’ future wishes and fears in their acculturation process     

Elena Briones  Carmen Tabernero  Alicia Arenas 《Int J Intercult Relat》2011,35(1):1-8

In this study we aim at learning about adolescents’ future fears and wishes from different cultural origins with a view to finding implications for intervention in the furthering of their satisfaction and social integration in their acculturation process. The study sample comprised 938 secondary school students—64.4% Spanish, 19.7% South American and 12.8% African. Wishes and fears were evaluated by means of two open questions and the answers were categorized following traditional content analysis methodology. Results show that the adolescents wish for a satisfactory job, family and economic situation, and that they are concerned about aspects such as their own health and that of their family, the possibility of having a precarious job, isolation from their family of origin, and not forming a family. The findings also allow us to give differential profiles according to the cultural origin of the adolescents.  相似文献   

Effects of cold water immersion on the recovery of physical performance and muscle damage following a one-off soccer match     

Ascensão A  Leite M  Rebelo AN  Magalhäes S  Magalhäes J 《Journal of sports sciences》2011,29(3):217-225

The aim of this study was to assess the effects of a single session of cold or thermoneutral water immersion after a one-off match on muscular dysfunction and damage in soccer players. Twenty-male soccer players completed one match and were randomly divided into cryotherapy (10 min cold water immersion, 10°C, n = 10) and thermoneutral (10 min thermoneutral water immersion, 35°C, n = 10) groups. Muscle damage (creatine kinase, myoglobin), inflammation (C-reactive protein), neuromuscular function (jump and sprint abilities and maximal isometric quadriceps strength), and delayed-onset muscle soreness were evaluated before, within 30 min of the end, and 24 and 48 h after the match. After the match, the players in both groups showed increased plasma creatine kinase activity (30 min, 24 h, 48 h), myoglobin (30 min) and C-reactive protein (30 min, 24 h) concentrations. Peak jump ability and maximal strength were decreased and delayed-onset muscle soreness increased in both groups. However, differential alterations were observed between thermoneutral water and cold water immersion groups in creatine kinase (30 min, 24 h, 48 h), myoglobin (30 min), C-reactive protein (30 min, 24 h, 48 h), quadriceps strength (24 h), and quadriceps (24 h), calf (24 h) and adductor (30 min) delayed-onset muscle soreness. The results suggest that cold water immersion immediately after a one-off soccer match reduces muscle damage and discomfort, possibly contributing to a faster recovery of neuromuscular function.  相似文献   

Differentiating experts' anticipatory skills in beach volleyball     

Cañal-Bruland R  Mooren M  Savelsbergh GJ 《Research quarterly for exercise and sport》2011,82(4):667-674

In this study, we examined how perceptual-motor expertise and watching experience contribute to anticipating the outcome of opponents' attacking actions in beach volleyball. To this end, we invited 8 expert beach volleyball players, 8 expert coaches, 8 expert referees, and 8 control participants with no beach volleyball experience to watch videos of attack sequences that were occluded at three different times and to predict the outcome of these situations. Results showed that expert players and coaches (who were both perceptual-motor experts) outperformed the expert referees (who were watching experts but did not have the same motor expertise) and the control group in the latest occlusion condition (i.e., at spiker-ball contact). This finding suggests that perceptual-motor expertise may contribute to successful action anticipation in beach volleyball.  相似文献   

Public libraries embrace citizen science: Strengths and challenges     

Anna Cigarini  Isabelle Bonhoure  Julián Vicens  Josep Perelló 《Library & information science research》2021,43(2):101090

Can public libraries become hubs for citizen science? In line with the principles of citizen science, this question was answered collaboratively with librarians from the Barcelona Network of Public Libraries who performed two hands-on activities. One activity was a training course taken by 30 librarians from 24 different libraries which enabled them to envisage citizen science implementation at each library. The other activity consisted of co-creating a citizen social science project and involved 40 library users, seven librarians from three different cities, and professional scientists. The analysis considers the perspectives of both librarians and users, through participant observation, surveys, and a focus group to identify strengths and challenges. The overall results suggest that public libraries can offer leadership in the promotion of citizen science and contribute to the mission of public libraries to act as local community hubs. The main challenges identified were related to the complexity of collaboration, uncertainty regarding research co-creation, and participant retention strategies.  相似文献   

Citizen reactions to municipalities’ Instagram communication     

David Perea  Enrique Bonsón  Michaela Bednárová 《Government Information Quarterly》2021,38(3):101579

In this paper, we explore how local governments are using Instagram as a communication tool to engage with their citizens, using data from the municipalities of Andalusia (Spain). We seek to identify the determinants of local government use of Instagram, the determinants of activity in this channel and the determinants of citizen reactions in order to understand the influence of media types (picture, video or album) used in municipality posts, and to understand content type (what the post is about). Instaloader, an open source intelligence (OSINT) tool for Instagram, was applied. It made it possible to automatically extract all posts of the analysed municipalities (14,742 posts). These were later automatically analysed using R, an open source software. It was determined that of the 29 Andalusian local governments with the highest populations, only those that maintain an account on Instagram, totalling 17 municipalities (58.62%), would be part of the final analysis. Our findings demonstrate that when local governments have a high level of debt, they do not maintain and actively use Instagram accounts. We also found that quality of posts’ content is more important than quantity of followers, since there is no significant relationship between citizen reactions and the number of inhabitants of a municipality or the number of followers (audience), while there is a significant negative relationship between the number of posts (activity) and reactions. Our results also highlight that the level of reactions can be stimulated by certain media and content types.  相似文献   

Drivers of teachers’ intentions to use mobile applications to teach science     

Ateş  Hüseyin  Garzón  Juan 《Education and Information Technologies》2022,27(2):2521-2542

Education and Information Technologies - Using mobile applications in science education has proven to be effective as it adds multiple benefits including learning gains, motivation to learn, and...  相似文献   

An evolving Learning Management System for new educational environments using 2.0 tools     

Miguel Á. Conde  Francisco J. García-Peñalvo  María J. Rodríguez-Conde  Marc Alier  María J. Casany  Jordi Piguillem 《Interactive Learning Environments》2014,22(2):188-204

The tools used in learning processes are in a continuous state of flux. One of the most significant changes is the application of Information and Communications technologies (ICTs) to educational contexts. This provides new possible ways to carry out learning activities, new learning services, the possibility to use new kinds of contents and activities, etc. However, ICTs have not provided as many advantages as they were supposed to, so changes are necessary. In this context, a new set of tools, Web 2.0, offers a new way to understand the Web, in which the user is the centre. Further, users can cooperate in order to define contents. This concept is also applied in technology-mediated learning but there are important problems when one tries to integrate such tools and concepts with existing systems. This paper explores the integration of these tools in traditional learning environments, the various possibilities and their advantages and drawbacks. After that, an interoperability scenario is described and two experiences are presented to show how 2.0 tools can be integrated in learning activities, and its effect in educational process.  相似文献