Bandpass sorting of heterogeneous cells using a single surface acoustic wave transducer pair     

Gergely Simon  Caroline Busch  Marco A. B. Andrade  Julien Reboud  Jonathan M. Cooper  Marc P. Y. Desmulliez  Mathis O. Riehle  Anne L. Bernassau 《Biomicrofluidics》2021,15(1)

Separation and sorting of biological entities (viruses, bacteria, and cells) is a critical step in any microfluidic lab-on-a-chip device. Acoustofluidics platforms have demonstrated their ability to use physical characteristics of cells to perform label-free separation. Bandpass-type sorting methods of medium-sized entities from a mixture have been presented using acoustic techniques; however, they require multiple transducers, lack support for various target populations, can be sensitive to flow variations, or have not been verified for continuous flow sorting of biological cells. To our knowledge, this paper presents the first acoustic bandpass method that overcomes all these limitations and presents an inherently reconfigurable technique with a single transducer pair for stable continuous flow sorting of blood cells. The sorting method is first demonstrated for polystyrene particles of sizes 6, 10, and 14.5 μm in diameter with measured purity and efficiency coefficients above 75 ± 6% and 85 ± 9%, respectively. The sorting strategy was further validated in the separation of red blood cells from white blood cells and 1 μm polystyrene particles with 78 ± 8% efficiency and 74 ± 6% purity, respectively, at a flow rate of at least 1 μl/min, enabling to process finger prick blood samples within minutes.  相似文献   

Jack Thomas,Our Third Baby,Enters the World!     

Anne Stebbins 《The Journal of perinatal education》2014,23(2):62-64

Without the confidence and support of the medical team, this mother’s first birth was difficult and unsatisfying. She was able to give birth naturally, in the care of a midwife, with her second baby. With confidence in her ability to give birth and the wisdom that comes with experience, she is able to give birth naturally with her third baby in spite of being induced.  相似文献   

Inquiry-Based Science: Turning Teachable Moments into Learnable Moments     

Berit S. Haug 《Journal of Science Teacher Education》2014,25(1):79-96

This study examines how an inquiry-based approach to teaching and learning creates teachable moments that can foster conceptual understanding in students, and how teachers capitalize upon these moments. Six elementary school teachers were videotaped as they implemented an integrated inquiry-based science and literacy curriculum in their classrooms. In this curriculum, science inquiry implies that students search for evidence in order to make and revise explanations based on the evidence found and through critical and logical thinking. Furthermore, the curriculum material is designed to address science key concepts multiple times through multiple modalities (do it, say it, read it, write it). Two types of teachable moments were identified: planned and spontaneous. Results suggest that the consolidation phases of inquiry, when students reinforce new knowledge and connect their empirical findings to theory, can be considered as planned teachable moments. These are phases of inquiry during which the teacher should expect, and be prepared for, student utterances that create opportunities to further student learning. Spontaneous teachable moments are instances when the teacher must choose to either follow the pace of the curriculum or adapt to the students’ need. One implication of the study is that more teacher support is required in terms of how to plan for and effectively utilize the consolidation phases of inquiry.  相似文献   

Exploring the Relations between In-service Training,Prior Contacts and Teachers’ Attitudes towards Persons with Intellectual Disability     

Rachel Sermier Dessemontet  Diane Morin  Anne G. Crocker 《International Journal of Disability, Development & Education》2014,61(1):16-26

This study investigates the relations between teachers’ attitudes towards persons with intellectual disability (ID), in-service training on ID, and prior contacts with persons with ID. A sample of Canadian elementary school teachers (N?=?118) completed the Attitudes Toward Intellectual Disability Questionnaire, which measures cognitive, affective and behavioural components of attitudes towards persons with ID. Correlational and multivariate regression analyses were performed. Frequent contacts with persons with ID and experience of including a child with ID increased the likelihood of reporting prior positive contacts with persons with ID. In turn, this was associated with less discomfort towards persons with ID and more willingness towards interactions. In-service training on ID was related to better knowledge of rights and capabilities of persons with ID. Experience of including a child with ID in general education classrooms increased the likelihood to report feeling competent in teaching children with ID, which predicted more willingness to include children with ID.  相似文献   

Supporting pre-service teachers to enhance the effectiveness of their classroom practice through engaging with the ‘voice’ of their pupils     

Elizabeth Anne Hopkins 《Teacher Development》2014,18(1):15-28

The research reported here was focussed on accessing the perceptions of pre-service teachers regarding the critical factors which support highly effective learning in schools in the United Kingdom. Their perceptions were arranged into a taxonomy so that they can be used as a starting point for their reflections on the observations they make of teaching and learning in schools with the aim of enhancing the effectiveness of their own teaching. The choice of technique used to access the perceptions was driven by the desire to provide the pre-service teachers with a method that they could use themselves to engage with the ‘voice’ of their own pupils regarding the factors which support effective learning. The engagement of pupil voice has become increasingly significant in understanding effective pedagogy and in securing more personalised approaches to learning in United Kingdom school contexts.  相似文献   

‘It gets narrower’: creative strategies for re-broadening queer peer education     

Anne Harris  David Farrington 《Sex education》2014,14(2):144-158

Using collaborative performance ethnography in community- and school-based settings, sex education has the potential to challenge at-risk narratives for lesbian, gay, bisexual, transgender, intersex and queer (LGBTIQ) youth. This paper problematises the youth-led drama project Epic Queer to test the ‘queer’ potential of youth-driven initiatives at the school and community level, and to reject the singularity of victimised and ‘at-risk’ narratives so pervasive in sex education internationally about queer youth. By drawing on the It Gets Better Project as an example of widespread but narrowing social media texts encouraging normativity, deferred pleasure and a happiness narrative, this paper argues for the potential of performance-based arts engagement for re-expanding queer youth subjectivities.  相似文献   

Elite und Exzellenz aus der Perspektive von Jugendlichen und ihren Peers an exklusiven Schulen     

Heinz-Hermann Krüger  Catharina Keßler  Ariane Otto  Anne Schippling 《Zeitschrift für Erziehungswissenschaft》2014,17(3):221-241

Against the backdrop of the recent political discourse on elite and excellence in the German education system, this paper examines how such constructions manifest themselves in programmatic concepts of schools and in the orientations of their pupils and their peers. They are reconstructed on the basis of a qualitative research project on educational careers of young people. This paper focuses on pupils and their peers at an elite school of sports and an international school. Firstly, the state of research on those types of school as well as on educational careers of their pupils will be presented. Secondly, the theoretical reference points and the methodological design of the study will be introduced. At the core of the paper is the analysis of the education related orientations of selected cases of young people and their peers: How do they position themselves towards elite and excellence in their individual and collective orientations and how is this relevant for processes of distinction and coherence building in the groups? In the resuming part of the paper, the results will be summarised and related to the state of research and the scientific discourse on elite and excellence.  相似文献   

Anatomy in occupational therapy program curriculum: Practitioners' perspectives          下载免费PDF全文

Katherine Anne Schofield 《Anatomical sciences education》2014,7(2):97-106

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Determination of clinically relevant content for a musculoskeletal anatomy curriculum for physical medicine and rehabilitation residents          下载免费PDF全文

Kristina Lisk  John F. Flannery  Eldon Y. Loh  Denyse Richardson  Anne M.R. Agur  Nicole N. Woods 《Anatomical sciences education》2014,7(2):135-143

To address the need for more clinical anatomy training in residency education, many postgraduate programs have implemented structured anatomy courses into their curriculum. Consensus often does not exist on specific content and level of detail of the content that should be included in such curricula. This article describes the use of the Delphi method to identify clinically relevant content to incorporate in a musculoskeletal anatomy curriculum for Physical Medicine and Rehabilitation (PM&R) residents. A two round modified Delphi involving PM&R experts was used to establish the curricular content. The anatomical structures and clinical conditions presented to the expert group were compiled using multiple sources: clinical musculoskeletal anatomy cases from the PM&R residency program at the University of Toronto; consultation with PM&R experts; and textbooks. In each round, experts rated the importance of each curricular item to PM&R residency education using a five‐point Likert scale. Internal consistency (Cronbach's alpha) was used to determine consensus at the end of each round and agreement scores were used as an outcome measure to determine the content to include in the curriculum. The overall internal consistency in both rounds was 0.99. A total of 37 physiatrists from across Canada participated and the overall response rate over two rounds was 97%. The initial curricular list consisted of 361 items. After the second iteration, the list was reduced by 44%. By using a national consensus method we were able to objectively determine the relevant anatomical structures and clinical musculoskeletal conditions important in daily PM&R practice. Anat Sci Educ 7: 135–143. © 2013 American Association of Anatomists.  相似文献   

Combining fiber dissection,plastination, and tractography for neuroanatomical education: Revealing the cerebellar nuclei and their white matter connections          下载免费PDF全文

Hisse Arnts  Michiel Kleinnijenhuis  Jan G.M. Kooloos  Annelieke N. Schepens‐Franke  Anne‐Marie van Cappellen van Walsum 《Anatomical sciences education》2014,7(1):47-55

In recent years, there has been a growing interest in white matter anatomy of the human brain. With advances in brain imaging techniques, the significance of white matter integrity for brain function has been demonstrated in various neurological and psychiatric disorders. As the demand for interpretation of clinical and imaging data on white matter increases, the needs for white matter anatomy education are changing. Because cross‐sectional images and formalin‐fixed brain specimens are often insufficient in visualizing the complexity of three‐dimensional (3D) white matter anatomy, obtaining a comprehensible conception of fiber tract morphology can be difficult. Fiber dissection is a technique that allows isolation of whole fiber pathways, revealing 3D structural and functional relationships of white matter in the human brain. In this study, we describe the use of fiber dissection in combination with plastination to obtain durable and easy to use 3D white matter specimens that do not require special care or conditions. The specimens can be used as a tool in teaching white matter anatomy and structural connectivity. We included four human brains and show a series of white matter specimens of both cerebrum and cerebellum focusing on the cerebellar nuclei and associated white matter tracts, as these are especially difficult to visualize in two‐dimensional specimens and demonstrate preservation of detailed human anatomy. Finally, we describe how the integration of white matter specimens with radiological information of new brain imaging techniques such as diffusion tensor imaging tractography can be used in teaching modern neuroanatomy with emphasis on structural connectivity. Anat Sci Educ. 7: 47–55. © 2013 American Association of Anatomists.  相似文献