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Little is known about the reliability, validity and smallest detectable differences of selected kinetic and temporal variables recorded by the Zebris FDM-THQ instrumented treadmill especially during running. Twenty male participants (age = 31.9 years (±5.6), height = 1.81 m (±0.08), mass = 80.2 kg (±9.5), body mass index = 24.53 kg/m2 (±2.53)) walked (5 km/h) and ran (10 and 15 km/h) on an instrumented treadmill, wearing running shoes fitted with Pedar-X insoles. A test-double retest protocol was conducted over two consecutive days. Maximal vertical force (Fmax), contact time (CT) and flight time (FT) data from 10 consecutive steps were collected. Within- and between-day reliability, smallest detectable differences (SDD) and validity (95% limits of agreement (LOA)) were calculated. ICC values for the Zebris for Fmax were acceptable (ICC ≥ 0.7) while CT and FT reliability indices were predominantly good (ICC ≥ 0.8) to excellent (ICC ≥ 0.9). The Zebris significantly underestimated Fmax when compared with the Pedar-X. The 95% LOA increased with speed. SDD ranged between 96 N and 169 N for Fmax, 0.017s and 0.055s for CT and 0.021s and 0.026s for FT. In conclusion, Zebris reliability was acceptable to excellent for the variables examined, but inferior in comparison with Pedar-X. With increased running speeds, a bias effect (underestimation) existed for the Zebris compared with Pedar-X.  相似文献   
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The mechanical behavior of cells offers insight into many aspects of their properties. We propose an approach to the mechanical analysis of cells that uses a combination of electromanipulation for stimulus and capacitance for sensing. To demonstrate this approach, polystyrene spheres and yeast cells flowing in a 25 μm×100 μm microfluidic channel were detected by a perpendicular pair of gold thin film electrodes in the channel, spaced 25 μm apart. The presence of cells was detected by capacitance changes between the gold electrodes. The capacitance sensor was a resonant coaxial radio frequency cavity (2.3 GHz) coupled to the electrodes. The presence of yeast cells (Saccharomyces cerevisiae) and polystyrene spheres resulted in capacitance changes of approximately 10 and 100 attoFarad (aF), respectively, with an achieved capacitance resolution of less than 2 aF in a 30 Hz bandwidth. The resolution is better than previously reported in the literature, and the capacitance changes are in agreement with values estimated by finite element simulations. Yeast cells were trapped using dielectrophoretic forces by applying a 3 V signal at 1 MHz between the electrodes. After trapping, the cells were displaced using amplitude and frequency modulated voltages to produce modulated dielectrophoretic forces. Repetitive displacement and relaxation of these cells was observed using both capacitance and video microscopy.  相似文献   
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