Thermally multiplexed polymerase chain reaction     

Christopher R. Phaneuf  Nikita Pak  D. Curtis Saunders  Gregory L. Holst  Joav Birjiniuk  Nikita Nagpal  Stephen Culpepper  Emily Popler  Andi L. Shane  Robert Jerris  Craig R. Forest 《Biomicrofluidics》2015,9(4)

Amplification of multiple unique genetic targets using the polymerase chain reaction (PCR) is commonly required in molecular biology laboratories. Such reactions are typically performed either serially or by multiplex PCR. Serial reactions are time consuming, and multiplex PCR, while powerful and widely used, can be prone to amplification bias, PCR drift, and primer-primer interactions. We present a new thermocycling method, termed thermal multiplexing, in which a single heat source is uniformly distributed and selectively modulated for independent temperature control of an array of PCR reactions. Thermal multiplexing allows amplification of multiple targets simultaneously—each reaction segregated and performed at optimal conditions. We demonstrate the method using a microfluidic system consisting of an infrared laser thermocycler, a polymer microchip featuring 1 μl, oil-encapsulated reactions, and closed-loop pulse-width modulation control. Heat transfer modeling is used to characterize thermal performance limitations of the system. We validate the model and perform two reactions simultaneously with widely varying annealing temperatures (48 °C and 68 °C), demonstrating excellent amplification. In addition, to demonstrate microfluidic infrared PCR using clinical specimens, we successfully amplified and detected both influenza A and B from human nasopharyngeal swabs. Thermal multiplexing is scalable and applicable to challenges such as pathogen detection where patients presenting non-specific symptoms need to be efficiently screened across a viral or bacterial panel.  相似文献   

Undisclosed Flexibility in Computing and Reporting Structural Equation Models in Communication Science     

Christopher Scott Seaman  Rene Weber 《Communication methods and measures》2015,9(4):208-232

The use of structural equation modeling (SEM) in communication research has become increasingly popular in recent years, with SEM being commonly used as the primary method of data analysis in studies published within the field’s journals. However, despite its widespread use, there are persistent misapplications of SEM in published empirical studies in the communication sciences. Of these types of misuse, the most troubling are those related to the exploitation of flexibilities in data collection and analysis. Given the number of decisions that must be made when analyzing and reporting structural equation models, decisions which often remain undisclosed, the potential for researchers to exploit flexibilities in SEM research is high. To assess the extent of this problem, as well as the more longstanding types of misuse (i.e., omission of information and disclosed “bad practice”) identified by prior studies, we undertake a methodical review of studies employing structural equation modeling within major journals in the field of communication between the years 2007 and 2011. Articles were coded on the characteristics of the sample, characteristics of the model tested, as well as for various potential problems that we defined a priori. The most serious issues are identified, and recommendations for future standards in SEM research are suggested.  相似文献   

The evolving reference desk: A case study     

Christopher Ross Bowron  Joseph E. Weber 《The Journal of Academic Librarianship》2019,45(5):102057

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¡No lucro! … from protest catch cry to presidential policy: is this the beginning of the end of the Chilean neoliberal model of education?     

Christopher Tome 《Journal of educational administration and history》2015,47(2):193-208

This paper surveys the delivery of school education in Chile over the last half-century. It focuses on evidence provided by recent academic studies on the impact of neoliberal education policies introduced by the military regime in the 1980s, and continued by successive democratic governments. It offers insights into recent popular critiques of the neoliberal model, as reported in the Chilean press, and reflected by significant popular support for student protest movements demanding the end of for-profit schools, and the revitalisation of the public school system – encapsulated in the protesters’ catch cry of ¡No lucro! It considers possible consequences of the adoption, as policy, of that catch cry, by the recently elected Bachelet government.  相似文献   

Children's literacy interest and its relation to parents’ literacy‐promoting practices          下载免费PDF全文

Laura E. Hume  Christopher J. Lonigan  Jessica D. McQueen 《Journal of Research in Reading》2015,38(2):172-193

This study examined how children's literacy interests related to parent literacy‐promoting practices across time. Using a sample of 909 preschool‐age children and the newly developed Child Activities Preference Checklist, literacy interest appeared to be a complex construct, not easily captured by a single measure. In a subsample of 230 children with longitudinal data, parent literacy practices and child literacy interests related concurrently and across time. Parent literacy practices were more stable than child literacy interests, with children's literacy interest continuing to develop over the preschool year. Parent practices of exposing children to literacy and teaching them literacy concepts appeared to be distinct constructs. Exposure to literacy was especially important in the growth of literacy interests and the hypothesis that exposure has a negative effect on children with little initial interest was not fully supported.  相似文献   

Genetic and Environmental Etiologies of the Longitudinal Relations Between Prereading Skills and Reading          下载免费PDF全文

Micaela E. Christopher  Jacqueline Hulslander  Brian Byrne  Stefan Samuelsson  Janice M. Keenan  Bruce Pennington  John C. DeFries  Sally J. Wadsworth  Erik Willcutt  Richard K. Olson 《Child development》2015,86(2):342-361

The present study explored the environmental and genetic etiologies of the longitudinal relations between prereading skills and reading and spelling. Twin pairs (n = 489) were assessed before kindergarten (M = 4.9 years), post‐first grade (M = 7.4 years), and post‐fourth grade (M = 10.4 years). Genetic influences on five prereading skills (print knowledge, rapid naming, phonological awareness, vocabulary, and verbal memory) were primarily responsible for relations with word reading and spelling. However, relations with post‐fourth‐grade reading comprehension were due to both genetic and shared environmental influences. Genetic and shared environmental influences that were common among the prereading variables covaried with reading and spelling, as did genetic influences unique to verbal memory (only post‐fourth‐grade comprehension), print knowledge, and rapid naming.  相似文献   

Exploring Shifts in Middle School Learners’ Modeling Activity While Generating Drawings,Animations, and Computational Simulations of Molecular Diffusion   总被引：1，自引：1，他引：0  

Michelle H. Wilkerson-Jerde  Brian E. Gravel  Christopher A. Macrander 《Journal of Science Education and Technology》2015,24(2-3):396-415

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Technology integration coursework and finding meaning in pre-service teachers’ reflective practice     

Royce Kimmons  Brant G. Miller  Julie Amador  Christopher David Desjardins  Cassidy Hall 《Educational technology research and development : ETR & D》2015,63(6):809-829

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Teaching anti-racist counseling theories: Black liberation narrative therapy     

Natoya Hill Haskins  Janee’ Advent Harris  Janise Parker  Aishwarya Nambiar  Philippa Chin 《Counselor Education & Supervision》2023,62(4):355-367

Counseling theories created by White theorists have traditionally failed to consider the religious or spiritual experiences of Black clients. Integration of Black liberation theology and narrative therapy provides a novel approach to support counseling trainees in meeting the needs of Black clients. Decolonizing therapeutic strategies are presented along with counselor educator recommendations.  相似文献   

‘Motivate’: the effect of a Football in the Community delivered weight loss programme on over 35-year old men and women’s cardiovascular risk factors     

Zoe Rutherford  Brendan Gough  Sarah Seymour-Smith  Christopher R Matthews  John Wilcox  Dan Parnell 《Soccer & Society》2014,15(6):951-969

The purpose of this study was to examine whether an innovative, inclusive and integrated 12-week exercise, behaviour change and nutrition advice-based weight management programme could significantly improve the cardiovascular risk factors of overweight and obese men and women over the age of 35. One hundred and ninety-four men and 98 women (mean age?=?52.28?±?9.74 and 51.19?±?9.04) attending a community-based intervention delivered by Notts County Football in the Community over one year, took part in the study. Height (m), weight (kg), fitness (meters covered during a 6?min walk) and waist circumference (cm) were measured at weeks 1 and 12 as part of the intervention. Changes in body weight, waist circumference and fitness for men and women were measured by a 2-way repeated measures ANOVA, with significance set to p?<?0.05.Weight, waist circumference and fitness significantly improved over time in both men (4.96?kg, 6.29?cm, 70.22?m; p?<?0.05) and women (4.26?kg, 5.90?cm, 35.29?m; p?<?0.05). The results demonstrated that the FITC lead weight loss intervention was successful in significantly improving cardiovascular risk factors in both men and women. In particular, the weight loss reductions achieved were comparable to those seen in similar, more costly men-only programmes. This is the first study to demonstrate the efficacy of such an intervention in an inclusive, mixed gender programme and more specifically, in women.  相似文献