Microfluidic size separation of cells and particles using a swinging bucket centrifuge     

Joo Chuan Yeo  Zhiping Wang  Chwee Teck Lim 《Biomicrofluidics》2015,9(5)

Biomolecular separation is crucial for downstream analysis. Separation technique mainly relies on centrifugal sedimentation. However, minuscule sample volume separation and extraction is difficult with conventional centrifuge. Furthermore, conventional centrifuge requires density gradient centrifugation which is laborious and time-consuming. To overcome this challenge, we present a novel size-selective bioparticles separation microfluidic chip on a swinging bucket minifuge. Size separation is achieved using passive pressure driven centrifugal fluid flows coupled with centrifugal force acting on the particles within the microfluidic chip. By adopting centrifugal microfluidics on a swinging bucket rotor, we achieved over 95% efficiency in separating mixed 20 μm and 2 μm colloidal dispersions from its liquid medium. Furthermore, by manipulating the hydrodynamic resistance, we performed size separation of mixed microbeads, achieving size efficiency of up to 90%. To further validate our device utility, we loaded spiked whole blood with MCF-7 cells into our microfluidic device and subjected it to centrifugal force for a mere duration of 10 s, thereby achieving a separation efficiency of over 75%. Overall, our centrifugal microfluidic device enables extremely rapid and label-free enrichment of different sized cells and particles with high efficiency.  相似文献   

Ideology,class and rationality: a critique of Cambridge International Examinations’ Thinking Skills curriculum     

Leonel Lim 《Cambridge Journal of Education》2012,42(4):481-495

This article undertakes a critique of the aims and objectives of Thinking Skills, one of the most widely and internationally used curricula in the teaching of thinking, offered by the University of Cambridge International Examinations. By engaging in a critical discourse analysis of how political and class biases are (re-)produced in the forms of thinking that are valued in the classroom, issues of power and ideology latent in curricular discourses of rationality are foregrounded. Specifically, it will be demonstrated that the text engages in the ideological project of shaping our commonsense understandings of what thinking and rationality is and should be, not only in instrumental forms that both connect to neoliberal commitments and privilege a particular fraction of the middle class, but also in ways that evacuate substantive notions of morality, social justice, and the common good from individuals’ deliberations. The article concludes by pointing to alternative conceptualizations of thinking curricula.  相似文献   

Body,authority and history - a photo essay     

Lim Ok-Sang  Park Bul-Dong 《Inter-Asia Cultural Studies》2013,14(3):457-460

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Singaporean Parents’ Curriculum Priorities for Their Children with Disabilities     

Levan Lim  Tan Ai Girl  Marilyn M. Quah 《International Journal of Disability, Development & Education》2000,47(1):77-87

This study examined Singaporean parents’ perspectives on how much they valued major curriculum skill areas for their children with disabilities. Parents were also asked to indicate whether they expected priority skill items within the curriculum areas to be performed with assistance or independently. The results showed that the parents of children with moderate and severe disabilities indicated the highest priority for self-help functional life skills, followed by community-based functional life skills, social relationship skills, and functional academics. Parents of children with mild disabilities indicated the highest priority for self-help functional life skills, followed by community-based functional skills, functional academics, and social relationship skills. The results also showed that parents’ relative ratings of the other skill areas besides self-help skills were influenced by the level of disability of their children. The milder the disability, the higher the relative parental ratings and expectations for independent performance of social relationship skills, functional academics, and community-based life skills. Conversely, the more severe the disability, the lower the relative ratings of these skills and expectations for independent performance compared with self-help functional life skills.  相似文献   

Quality Assurance in Higher Education in Developing Countries     

David Lim 《Assessment & Evaluation in Higher Education》1999,24(4):379-390

In recent years universities in developing countries have followed their counterparts in developed countries in adopting quality assurance to improve the quality of their teaching, research and direct community service programmes. While many of the conditions required for the successful implementation of quality assurance programmes are not present in most universities in developing countries, their adoption will still be useful. Such programmes show how a university's seemingly disparate activities are related to one another to serve a common cause and how the quality of these can best be improved by adopting an integrated approach. In the process, they provide more focus and direction to the work of the traditional academic committee system. However, the quality assurance programmes must be modified to suit the conditions prevailing in developing countries, by being simple in design, modest in expectations, and realistic in requirements.  相似文献   

Educational Assessment in Singapore     

Elaine P. Y. Lim  Annie Tan 《Assessment in Education: Principles, Policy & Practice》1999,6(3):391-404

Educational assessment in the Singapore context is shaped, to a large extent, by the country's educational policies and programmes, as well as the general principles and practices of assessment. This paper presents a profile of the assessment system in Singapore and how it complements the education system to realise the fullest potential of its precious human resources. National examinations and school-based assessments at each milestone in the education system are described so as to highlight the distinctive features of educational assessment in Singapore.  相似文献   

The Diffusion and Transmission of Football in the Straits Settlements and Malay States, 1874–1899: Early Inter-Settlement Games and Inter-State Competition Among European Clubs and Teams     

Lim Peng Han  Mohd Salleh Aman 《国际体育史杂志》2018,35(12-13):1335-1355

Abstract

Since 1786 the British occupied Penang, Malacca, and Singapore known as the Straits Settlements. They began indirect rule in Selangor, Perak, Negri Sembilan, and Pahang to form the Federated Malay States in 1896. Cricket and later, football was played by the military and European clubs like the Singapore Cricket Club, the Penang Cricket Club, Selangor Club Lake Club, and Perak Club. The Chinese and Malay in the Straits formed their clubs along ethnic lines. The outstation Malay States clubs of the Kajang Recreation Club, Klang Club, and Malay States Guides had a mixed team of European and Natives. The football tournaments in Singapore were exclusively for Europeans. While the Rodger Football Cup in Selangor was for various non-European teams located in the many districts around Kuala Lumpur. After the railway lines began linking in land towns of Kuala Lumpur, Taiping to Seremban to their respective ports, football teams travelled to other settlements to play matches, facilitating the growth of inter-settlement matches. It enabled the Federated Malay States to organized the first inter-state league in 1899. The diffusion and transmission of football in the Malay States should be seen in context social and political changes in the Malaya Peninsula and its economic growth.  相似文献   

Impacting the Positioning of Teaching as Life-long Career     

Lim Lee Hean 《Educational Research for Policy and Practice》2003,2(2):87-92

This article attempts to provide an overview of relevant recent initiates undertaken in Singapore to attract and retain school teachers. A teaching track in the new career structure seeks to recognise and provide opportunities for teachers who prefer to serve life-long as teachers instead of being promoted to administrative positions. Coupled with this is the draw of financial incentive in the new recognition structure to reward the good ones who have the stamina to stay, and a competitive gross starting salary to entice new entrants into the profession. The author presents the thrust in education of the country as a means to an economic end and surfaces inherent contradictions. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Use of NSE/PS2m-transgenic mice in the study of the protective effect of exercise on Alzheimer's disease     

Cho JY  Hwang DY  Kang TS  Shin DH  Hwang JH  Lim CH  Lee SH  Lim HJ  Min SH  Seo SJ  Song YS  Nam KT  Lee KS  Cho JS  Kim YK 《Journal of sports sciences》2003,21(11):943-951

In its late stage, Alzheimer's disease results in progressive muscle weakness in the arms and legs. The aim of this study was to determine whether mice expressing the skeletal muscle-specific mutant PS2 gene (a model of Alzheimer's disease) are a useful experimental system to study the protective effect of exercise on A beta-42 reduction, improvement of behavioural function and changes in metabolic parameters. With this aim in mind, the transgenic mice were subjected to treadmill exercise for 3 months. The results showed that in transgenic mice, but not in normal mice, treadmill exercise resulted in a reduction of A beta-42 deposits and an improvement in behavioural function, thereby restoring normal concentrations of total cholesterol, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol and triglyceride. Thus, exercise may represent a practical therapeutic strategy for use with human patients with Alzheimer's disease.  相似文献   

Inclusive Education     

Levan Lim  Rebecca Ireland 《International Journal of Disability, Development & Education》2001,48(3):313-316

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