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Dietmar Enko Gernot Kriegsh?user Robert Stolba Elfriede Worf Gabriele Halwachs-Baumann 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2015,25(2):203-212
Introduction
Recently several diagnostic manufacturers have launched new 25-hydroxy-vitamin D (25[OH]D) assays, which are aligned to the National Institute of Standards and Technology (NIST) Standard Reference Materials (SRM) (NIST, Gaithersburg, Maryland). The aim of this study was to compare the performance of one liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, one enzyme linked immunosorbent assay (ELISA), and one recalibrated and previous version of a chemiluminescence immunoassay (CLIA).Material and methods
Serum-aliquots of 198 patient samples from routine 25(OH)D analysis were measured by the ClinMass® LC-MS/MS Complete Kit (RECIPE Chemicals + Instruments GmbH, Munich, Germany), the ORGENTEC 25(OH)D3/D2 ELISA (ORGENTEC Diagnostika GmbH, Mainz, Germany), the recalibrated Immunodiagnostic Systems (IDS)-iSYS 25(OH)DS and the previous used IDS-iSYS 25(OH)D CLIA (Immunodiagnostic Systems Ltd, Boldon, United Kingdom). Bland-Altman and Deming regression analyses were calculated for methods comparison of all tested 25(OH)D assays. The LC-MS/MS method was defined as the reference method. Within-run and between-run precision measurements were performed for all methods with three different concentration levels.Results
Compared to the LC-MS/MS method, the new IDS-iSYS 25(OH)DS and ORGENTEC 25(OH)D3/D2 assay demonstrated mean relative biases of 16.3% and 17.8%. The IDS-iSYS 25(OH)D assay showed the lowest mean bias of 1.5%. Deming regression analyses of the recalibrated IDS-iSYS 25(OH)DS and the ORGENTEC 25(OH)D3/D2 assay showed proportional differences, when compared to the reference method. All assays showed a within-run and between-run imprecision of ≤ 20% at each of the evaluated concentration levels.Conclusions
The evaluated standardized immunoassays and LC-MS/MS are useful methods for measuring 25(OH)D serum-levels in clinical laboratories.Key words: vitamin D, immunoassays, liquid chromatography-tandem mass spectrometry, reference standards, quality improvement 相似文献244.
Educational Studies in Mathematics - Modelling competencies are currently included in numerous curricula worldwide and are generally accepted as a complex, process-oriented construct. Therefore,... 相似文献
245.
Expert and preservice secondary teachers’ competencies for noticing student thinking about modelling
Cai Jinfa LaRochelle Raymond Hwang Stephen Kaiser Gabriele 《Educational Studies in Mathematics》2022,109(2):431-453
Educational Studies in Mathematics - This study examined how expert and novice (preservice) teachers solved mathematical modelling tasks as well as how they noticed written artifacts of student... 相似文献
246.
Tekin-Sitrava Reyhan Kaiser Gabriele Işıksal-Bostan Mine 《International Journal of Science and Mathematics Education》2022,20(7):1611-1634
International Journal of Science and Mathematics Education - Although the effects of professional development programs on teachers’ noticing skills have attracted considerable interest among... 相似文献
247.
Cevikbas Mustafa Kaiser Gabriele 《International Journal of Science and Mathematics Education》2022,20(7):1455-1480
International Journal of Science and Mathematics Education - Many students are becoming increasingly disengaged in school, particularly in mathematics. This is an important problem as lack of... 相似文献
248.
Interpretation is defined as an activity that “facilitates an intellectual and emotional connection between the interests of the visitor and the meanings of the resource.” The concept of helping visitors connect to meanings is a constant theme in the development of philosophies and practices in interpretation over the last century. Parks and museums have long shared in the effort to put this concept into practice. This article documents this history and the implications for contemporary interpretation. 相似文献