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A novel microfluidic device which consists of two stages for particle focusing and separation using a viscoelastic fluid has been developed. A circular capillary tube was used for three-dimensional particle pre-alignment before the separation process, which was inserted in a polydimethylsiloxane microchannel. Particles with diameters of 5 and 10 μm were focused at the centerline in the capillary tube, and the location of particles was initialized at the first bifurcation. Then, 5 and 10 μm particles were successfully separated in the expansion region based on size-dependent lateral migration, with ∼99% separation efficiency. The proposed device was further applied to separation of MCF-7 cells from leukocytes. Based on the cell size distribution, an approximate size cutoff for separation was determined to be 16 μm. At 200 μl/min, 94% of MCF-7 cells were separated with the purity of ∼97%. According to the trypan blue exclusion assay, high viability (∼90%) could be achieved for the separated MCF-7 cells. The use of a commercially available capillary tube enables the device to be highly versatile in dealing with particles in a wide size range by using capillary tubes with different inner diameters.  相似文献   
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We analyse the role of creative workers in the region as a source and foundational element of regional innovation in the European Union. We show the empirical relevance of this factor – which we label inspiration – within the structure of a recursive model of regional innovation for a set of 83 European regions. We show that, when differentiated from the presence of regional intelligence – as measured by the availability of human capital – and from technological infrastructure, inspiration, along with the degree of development of national and regional institutions, has the strongest direct and indirect effects on regional patenting activity.  相似文献   
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Major channels of communication are examined for two major functions: providing news of the ethnic community, and linking ethnics to the “mother country.” The four major channels are: interpersonal channels (friends and neighbors), radio, local metropolitan daily newspapers, and ethnic-language newspapers. Size of ethnic group is linked to channel preference, and greater ethnicity is related to a preference for ethnic media for both functions. Lower SES ethnics lend to rely more on interpersonal communication and ethnic media, while higher SES ethnics turn more to metro media and magazines. Channel preference as indicated by respondents is also linked to actual usage patterns.  相似文献   
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Newborns' face recognition: role of inner and outer facial features   总被引:1,自引:0,他引:1  
Existing data indicate that newborns are able to recognize individual faces, but little is known about what perceptual cues drive this ability. The current study showed that either the inner or outer features of the face can act as sufficient cues for newborns' face recognition (Experiment 1), but the outer part of the face enjoys an advantage over the inner part (Experiment 2). Inversion of the face stimuli disrupted recognition when only the inner portion of the face was shown, but not when the whole face was fully visible or only the outer features were presented (Experiment 3). The results enhance our picture of what information newborns actually process and encode when they discriminate, learn, and recognize faces.  相似文献   
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The continued growth of online programs in higher education has resulted in concerns about how institutions monitor the quality of their online programs. These concerns indicate a need for a process by which online programs may be evaluated and compared. They provided the impetus for this study, the goals of which were to identify quality indicators specific to community college online programs and to determine stakeholders' perceived importance of those indicators.

Categories of indicators were identified through a literature review, and these items were used to guide the development of a Delphi study to identify potential indicators. The results of the Delphi study were then used to create a three-part Stakeholder Survey designed to collect input on perceived levels of importance for each potential indicator using the magnitude estimation technique. The Stakeholder Survey was also used to validate the results of the Delphi. To refine the results of the Delphi study and Stakeholder Survey, a final survey was distributed to a group of distance learning experts, identified through their scholarly research and professional activity. The participants were asked to review the earlier results and classify each item as a factor, indicator, or other according to definitions provided.

The three research stages produced a set of potential data sources that a community college may want to pursue when assessing the effectiveness of its online programs and services. Each of the potential sources of data will be reviewed with a goal towards identifying specific indicators that may be used in developing future instruments.  相似文献   
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