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To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His(6)-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).  相似文献   
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In this study, we examined how perceptual-motor expertise and watching experience contribute to anticipating the outcome of opponents' attacking actions in beach volleyball. To this end, we invited 8 expert beach volleyball players, 8 expert coaches, 8 expert referees, and 8 control participants with no beach volleyball experience to watch videos of attack sequences that were occluded at three different times and to predict the outcome of these situations. Results showed that expert players and coaches (who were both perceptual-motor experts) outperformed the expert referees (who were watching experts but did not have the same motor expertise) and the control group in the latest occlusion condition (i.e., at spiker-ball contact). This finding suggests that perceptual-motor expertise may contribute to successful action anticipation in beach volleyball.  相似文献   
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The tools used in learning processes are in a continuous state of flux. One of the most significant changes is the application of Information and Communications technologies (ICTs) to educational contexts. This provides new possible ways to carry out learning activities, new learning services, the possibility to use new kinds of contents and activities, etc. However, ICTs have not provided as many advantages as they were supposed to, so changes are necessary. In this context, a new set of tools, Web 2.0, offers a new way to understand the Web, in which the user is the centre. Further, users can cooperate in order to define contents. This concept is also applied in technology-mediated learning but there are important problems when one tries to integrate such tools and concepts with existing systems. This paper explores the integration of these tools in traditional learning environments, the various possibilities and their advantages and drawbacks. After that, an interoperability scenario is described and two experiences are presented to show how 2.0 tools can be integrated in learning activities, and its effect in educational process.  相似文献   
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This paper focuses on institutionally powered personal learning environments (iPLEs). The concept of the iPLE can be seen as a way universities can incorporate learner-centred approach into the architecture of their technology-enhanced learning environments. The aim of this paper is to pose that there are other ways to learn complementary to virtual learning environments, such as using distributed Web 2.0 tools from personal learning environments (PLEs). With that aim in mind, this paper presents an exploratory study with two different iPLE configurations, analyses how they modify the learning–teaching process, and reports their perceived usefulness from the students' point of view. These two case studies were carried out by a research group of educational technology from a Spanish university in graduate and postgraduate courses. As reported by other case studies, the results revealed that students use the environment basically in the academic context and this usage is conceived as another time and effort requiring task. This fact can be observed in the low level iPLEs customization. The overall conclusions we can extract are, on the one hand, that the new environment to integrate informal and formal learning should be flexible and adaptable to the student's needs and preferences and, on the other hand, that academic tools might someday become personal tools.  相似文献   
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Videos can be used as didactic tools for self‐learning under several circumstances, including those cases in which students are responsible for the development of this resource as an audiovisual notebook. We compared students' and teachers' perceptions regarding the main features that an audiovisual notebook should include. Four questionnaires with items about information, images, text and music, and filmmaking were used to investigate students' (n = 115) and teachers' perceptions (n = 28) regarding the development of a video focused on a histological technique. The results show that both students and teachers significantly prioritize informative components, images and filmmaking more than text and music. The scores were significantly higher for teachers than for students for all four components analyzed. The highest scores were given to items related to practical and medically oriented elements, and the lowest values were given to theoretical and complementary elements. For most items, there were no differences between genders. A strong positive correlation was found between the scores given to each item by teachers and students. These results show that both students' and teachers' perceptions tend to coincide for most items, and suggest that audiovisual notebooks developed by students would emphasize the same items as those perceived by teachers to be the most relevant. Further, these findings suggest that the use of video as an audiovisual learning notebook would not only preserve the curricular objectives but would also offer the advantages of self‐learning processes. Anat Sci Educ 7: 209–218. © 2013 American Association of Anatomists.  相似文献   
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Different techniques and tools currently exist to generate three-dimensional models of small elements, buildings and cities. Apart from being easier to interpret than two-dimensional drawings, these models facilitate data necessary for reconstruction projects, preservation or rehabilitation of the architectural or archaeological heritage. Traditional surveying only enables us to obtain discrete information of the characteristic lines that define each surface, edge, slope change etc. whereas work with massive capture techniques allows us to obtain continuous points of the surfaces. Photogrammetry or laser scanning combined with conventional photography provides information of the building in addition to its geometry. We carried out a review of the different techniques showing the advantages and disadvantages of each technique as well as the information that can be obtained by applying these techniques to the survey of the great Gate of Antioch in the town of Aleppo, declared a World Heritage Site by UNESCO in 1986, in Syria.  相似文献   
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This study aims to show how the integration of passive and active learning approaches contributes towards the significant understanding of problems associated with sustainable development. To do this, a case study from the municipality of San Miguel de Achiutla in Oaxaca, Mexico, is used as a general method, with the specific procedure including an instrument based on secondary sources representing the passive learning phase, as well as an instrument created on the basis of primary sources, designed in relation to the active learning component. The passive learning phase enabled us to identify variables pertaining to the highly vulnerable dimensions of sustainable development, and to also detect areas in which there is a deficit of information, since not all data are available, while the active learning phase revealed the causes of vulnerability from the perspective of different agents, including those who experienced the issues directly. In view of the above, we conclude that rather than being exclusive, these two different approaches should in fact be complementary.  相似文献   
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