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Ramona C. Dolscheid-Pommerich Ute Klarmann-Schulz Rupert Conrad Birgit Stoffel-Wagner Berndt Zur 《Biochemia medica : ?asopis Hrvatskoga dru?tva medicinskih biokemi?ara / HDMB》2016,26(1):82-89
Introduction
Preanalytical specifications for urinalysis must be strictly adhered to avoid false interpretations. Aim of the present study is to examine whether the preanalytical factor ‘time point of analysis’ significantly influences stability of urine samples for urine particle and dipstick analysis.Materials and methods
In 321 pathological spontaneous urine samples, urine dipstick (Urisys™2400, Combur-10-Test™strips, Roche Diagnostics, Mannheim, Germany) and particle analysis (UF-1000 i™, Sysmex, Norderstedt, Germany) were performed within 90 min, 120 min and 240 min after urine collection.Results
For urine particle analysis, a significant increase in conductivity (120 vs. 90 min: P < 0.001, 240 vs. 90 min: P < 0.001) and a significant decrease in WBC (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), RBC (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), casts (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001) and epithelial cells (120 vs. 90 min P = 0.610, 240 vs. 90 min P = 0.041) were found. There were no significant changes for bacteria. Regarding urine dipstick analysis, misclassification rates between measurements were significant for pH (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), leukocytes (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), nitrite (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), protein (120 vs. 90 min P < 0.001, 240 vs. 90 min P<0.001), ketone (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), blood (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001), specific gravity (120 vs. 90 min P < 0.001, 240 vs. 90 min P < 0.001) and urobilinogen (120 vs. 90 min, P = 0.031). Misclassification rates were not significant for glucose and bilirubin.Conclusion
Most parameters critically depend on the time window between sampling and analysis. Our study stresses the importance of adherence to early time points in urinalysis (within 90 min).Key words: urinalysis, automation, analytical sample preparation methods, flow cytometry, specimen handling 相似文献32.
Kathleen HaleAuthor Vitae Ramona McNealAuthor Vitae 《Government Information Quarterly》2011,28(2):262-270
Since the advent of the internet, state governments have been challenged to balance the forces of technological change against fundamental decisions about governance and administration. This research examines that balance in the case of electronic commerce and its relationship to state budgets and revenue raised by sales tax. Under the voluntary Streamlined Sales and Use Tax Agreement of 2002, states can cooperate with one another and with sellers to collect sales tax on internet sales, yet only about half the states that levy sales tax have initiated this policy change. This research tests rival explanations for state membership in this interstate administrative agreement and implementation of its provisions from 2003 through 2007. State participation is enhanced by existing technological capacity and frustrated by requirements for shared governance and shared administrative responsibilities. Participation is fostered by more extensive implementation of e-commerce and certain political factors. State fiscal factors and e-Government implementation are not significant. Moreover, state legislative professionalism inhibits state participation in this agreement. These findings have implications for understanding the influence of future technological change on government practices and for intergovernmental relationships weighted toward shared governance. 相似文献
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In order to determine primary health care providers' (PCPs) knowledge gaps on Parkinson's disease, data were collected before and after a one-hour continuing medical education (CME) lecture on early Parkinson's disease recognition and treatment from a sample of 104 PCPs participating at an annual meeting. The main outcome measure was the proportion of questions answered correctly by each PCP before the lecture. We measured the change in proportion of correct answers before and after the lecture (delta). Ninety-nine percent of the PCPs who attended the lecture returned the questionnaire. The level of knowledge on Parkinson's disease before the lecture was relatively low, particularly in management (61.4%) and diagnosis (34.4%). PCPs' perceived knowledge was not associated with the number of correct responses on management at baseline. Test scores significantly improved after the CME lecture. Our results show that PCPs' baseline knowledge of diagnosis and management of Parkinson's disease and self-perceived knowledge on this topic are relatively limited. Appropriately, United States reaccreditation programs do not only rely on self-perception. Longitudinal studies are needed to determine the impact of CME in knowledge retention and patient care in Parkinson's disease. 相似文献
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Abstract As early childhood teacher educators, we are thoroughly immersed in conversations about developmentally appropriate practice. Whether teachers should incorporate the use of food as learning materials is a case in point. As with many other concerns we ponder in our field, our task must be to define, clarify, and consider multiple perspectives in the pursuit of a rational justification of our position. In this paper we report the results of our careful review of theory and research that leads us to propose that using food as art material violates principles of best practice for three main reasons, because it: (1) teaches young children that it is acceptable to eat play materials and to play with food, (2) teaches young children that it is acceptable practice to waste limited resources, (3) violates many premises of multicultural education, or teaching children about diverse ethnic groups. 相似文献
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Silvio Lorenzetti Fabian Ammann Sabrina Windmüller Ramona Häberle Sören Müller Micah Gross 《Sports biomechanics / International Society of Biomechanics in Sports》2019,18(1):63-74
As hill jumps are very time-consuming, ski jumping athletes often perform various imitation jumps during training. The performed jumps should be similar to hill jumps, but a direct comparison of the kinetic and kinematic parameters has not been performed yet. Therefore, this study aimed to correlate 11 common parameters during hill jumps (Oberstdorf Germany), squat jumps (wearing indoor shoes), and various imitation jumps (rolling 4°, rolling flat, static; jumping equipment or indoor shoes) on a custom-built instrumented vehicle with a catch by the coach. During the performed jumps, force and video data of the take-off of 10 athletes were measured. The imitation and squat jumps were then ranked. The main difference between the hill jumps and the imitation and squat jumps is the higher maximal force loading rate during the hill jumps. Imitation jumps performed on a rolling platform, on flat ground were the most similar to hill jumps in terms of the force–time, and leg joint kinematic properties. Thus, non-hill jumps with a technical focus should be performed from a rolling platform with a flat inrun with normal indoor shoes or jumping equipment, and high normal force loading rates should be the main focus of imitation training. 相似文献