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31.
M. Prabha V. Ravi N. Ramachandra Swamy 《Indian journal of clinical biochemistry : IJCB》2013,28(3):283-291
The activity of six hydrolytic enzymes-carboxyl esterase, acid phosphatase, alkaline phosphatase, β-galactosidase, β-glucosidase and β-hexosaminidase, were studied in different regions of the normal human brain tissue obtained at autopsy. Protein estimation and activities of the hydrolytic enzymes with respective substrates were assayed by spectrophotometric and spectroflourometric methods. Amongst the eight regions of the brain-frontal, parietal, occipital, temporal, thalamus, cerebellum and hippocampus, the pineal gland showed highest activity for all hydrolytic enzymes studied except for carboxyl esterase. Among six hydrolases studied, hexosaminidase exhibited highest activity in all regions of the human brain while alkaline phosphatase activity was the least amongst all regions studied. A majority of the enzymes studied showed higher activity in gray matter as compared to the white matter except acid phosphatase and β-glucosidase which exhibited higher activity in the white matter. The most significant finding in the present study was the high activity of all hydrolytic enzymes noted in the pineal gland as compared to all other regions of the human brain. Such a finding has not been hitherto reported earlier in human brain tissue samples. If the specific activities of these enzymes are to be considered as any functional index, then pineal gland may be more metabolically active tissue with respect to the hydrolytic function as compared to the other regions of the brain. 相似文献
32.
Phenotypic Heterogeneity of Asian Indian Inversion Deletions Gγ(Aγδβ)0 Breakpoint A and Breakpoint B
Sanjay Pandey Sweta Pandey Ravi Ranjan Rahasyamani Mishra Monica Sharma Renu Saxena 《Indian journal of clinical biochemistry : IJCB》2013,28(1):98-101
Asian Indian inversion deletion Gγ (Aγδβ)0-thalassemia is a rare entities characterized by high HbF. Due to interaction with various genetic factors, patients with Gγ (Aγδβ)0-thalassemia showed clinical variability. Here we are presenting the phenotypic expression of Gγ(Aγδβ)0 thalassemia under influence of various co-inherited factors. Patient with α-globin gene deletion had mild phenotype than the patient with β-globin mutations. Patient with alpha gene deletion were presenting clinical character like thalassemia intermedia while Gγ (Aγδβ)0-thalassemia patients with co- presence of beta thalssemia mutation clinically behaved like thalassemia major. 相似文献
33.
Anitha Devanath Somdattaa Ray Ravi Kumar B. S. Prarthana 《Indian journal of clinical biochemistry : IJCB》2014,29(1):45-50
HIV infection is associated with lipid abnormalities in treatment naïve patients. CD4 count is used for monitoring the HIV infection. Primary objective was to evaluate and correlate lipid profile and CD4 counts in HIV infection. Secondary objective was to evaluate the feasibility of using Lipid profile to monitor the HIV infected treatment naïve patients instead of CD4 counts. 112 patients were selected based on a criteria from ART center in tertiary care center. CD4 counts were assessed and Lipid profile was evaluated enzymatically. A correlation study was done between the lipid profile and the CD4 count and clinical stages of infection. Cholesterol showed no significant correlation in any stage. HDL-C showed significant correlation (p < 0.05) with stage 2 and 4 disease. LDL-C showed no significant correlation in any stage. TGL showed significant correlation (p < 0.05) at stage 4 disease. Hence, HDL-C and TGL can be used as indicators of lipid status and for infection progression in treatment naive HIV patients, while Cholesterol and LDL-C has no role to play. 相似文献
34.
Mridula Mahajan Sukhraj Kaur Shruti Mahajan Ravi Kant 《Indian journal of clinical biochemistry : IJCB》2009,24(2):205-207
Uric acid an endogenous aqueous antioxidant in normal humans is present in much higher concentrations than vitamin C and has
been known to cover 2/3rd of the free radical scavenging capacity in plasma. In the present study average uric acid levels of patients of rheumatoid
arthritis were found to be close to the normal individuals. A unique feature was observed after classifying the patients on
the basis of the duration of suffering, the patients having longer duration of disease had least uric acid levels as compared
to those suffering from relatively lesser period, similar trend was observed in the ascorbic acid estimations. The decline
in uric acid values with progression of the disease was much more than what was observed in case of ascorbic acid suggesting
the significant role of uric acid in scavenging of free radicals. Too much lowering of Uric Acid should be checked and vitamin
C should be supplemented in diet for maintaining the balance between pro oxidant and antioxidant forces to check pro oxidant
insult in rheumatoid arthritis. 相似文献
35.
Despite the accepted need for strategic alignment in the manufacturing strategy literature, there has been relatively little research aimed at simultaneously aligning decisions in the structural and infrastructural areas with the competitive priorities of an organization. This article attempts to bridge the gap by examining how work force management practices, an infrastructural decision, should be aligned with both the process technology of a manufacturing plant, a structural decision, and its competitive priorities. A conceptual model for linking competitive priorities, process technologies, and work force management practices is presented, and specific and testable propositions are developed for future empirical research. The proposed conceptual model is based on the premise that the process of matching work force management practices to competitive priorities involves identification of the key managerial tasks underlying various competitive priorities. These tasks, in turn, are matched with the process technology characteristics and work force management practices to seek a good fit, which is expected to improve manufacturing performance. 相似文献
36.
A new method is demonstrated to transport particles, cells, and other microorganisms using rectified ac electro-osmotic flows in open microchannels. The rectified flow is obtained by synchronous zeta potential modulation with the driving potential in the microchannel. Experiments were conducted to transport both neutral, charged particles, and microorganisms of various sizes. A maximum speed of 50 μm∕s was obtained for 8 μm polystyrene beads, without any electrolysis, using a symmetrical square waveform driving electric field of 5 V∕mm at 10 Hz and a 360 V gate potential with its polarity synchronized with the driving potential (phase lag=0°). 相似文献
37.
The nematodes (worms) Caenorhabditiselegans and Caenorhabditisbriggsae are well-known model organisms to study the basis of animal development and behaviour. Their sinusoidal pattern of movement is highly stereotypic and serves as a tool to monitor defects in neurons and muscles that control movement. Until recently, a simple yet robust method to initiate movement response on-demand did not exist. We have found that the electrical stimulation in a microfluidic channel, using constant DC electric field, induces movement (termed electrotaxis) that is instantaneous, precise, sensitive, and fully penetrant. We have further characterized this behaviour and, in this paper, demonstrate that electrotaxis can also be induced using a pulse DC electric signal. Worms responded to pulse DC signals with as low as 30% duty cycle by moving towards the negative electrode at the same speed as constant DC fields (average speed of C. elegans?=?296?±?43?μm/s and C. briggsae?=?356?±?20?μm/s, for both constant and pulse DC electric fields with various frequencies). C. briggsae was found to be more sensitive to electric signals compared to C. elegans. We also investigated the turning response of worms to a change in the direction of constant and pulse DC signals. The response for constant DC signal was found to be instantaneous and similar for most worms. However, in the case of pulse DC signal, alterations in duty cycle affected the turning response time as well as the number of responding worms. Our findings show that pulse DC method allows quantitative measurement of response behaviour of worms and suggest that it could be used as a tool to study the neuronal basis of such a behaviour that is not observed under constant DC conditions. 相似文献
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Alan Chalmers Lloyd Ackert Pnina Abir-Am Maria Trochatos Carolyn R. Miller Nicolas Rasmussen Roy Macleod Ingo Brigandt Lorna Barrow Ian Maclean Elof Axel Carlson Susan E. Cozzens Cindy Klestinec Patricia O'Grady Alison Bashford Jim Endersby Jonathan Simon David Mercer David Oldroyd Suzanne Verderber Peter Slezak Rachel Cooper Patricia Fara Ravi Arvind Palat Peder Anker Bernhard Fritscher David Branagan 《Metascience》2002,11(3):329-429
40.
Ravi Shankar Prasad Yadav Vishal Khatri Nitin Amdare Kalyan Goswami V. B. Shivkumar Nitin Gangane Maryada Venkata Rami Reddy 《Indian journal of clinical biochemistry : IJCB》2016,31(2):203-208
Helminths are known to modulate host’s immune system and understanding this modulation can help in identification of novel therapeutic agents for autoimmune diseases. In this study, we have assessed the immune-modulatory activity and the therapeutic effect of Brugia malayi recombinant cystatin (rBmCys) in methylated BSA (mBSA) induced arthritis using rodent model. Administration of rBmCys has suppressed the severity of mBSA-arthritis in mastomys by reducing paw swelling and other clinical disease parameters as evident from significantly decreased arthritic index. The anti-arthritic effect of rBmCys was also confirmed by decreased histopathological score for synovitis, bone erosion and fibrosis in the tissue sections of paws. Further, this therapeutic effect of cystatin was found to be associated with significantly decreased production of IFN-γ and TNF-α and increased release of IL-4 and IL-10 cytokines. These results implied that rBmCys treatment has alleviated mBSA-induced arthritis and thus can be a promising alternative agent for the treatment of arthritis. 相似文献