Development and validation of a low cost blood filtration element separating plasma from undiluted whole blood     

Homsy A  van der Wal PD  Doll W  Schaller R  Korsatko S  Ratzer M  Ellmerer M  Pieber TR  Nicol A  de Rooij NF 《Biomicrofluidics》2012,6(1):12804-128049

Clinical point of care testing often needs plasma instead of whole blood. As centrifugation is labor intensive and not always accessible, filtration is a more appropriate separation technique. The complexity of whole blood is such that there is still no commercially available filtration system capable of separating small sample volumes (10-100 μl) at the point of care. The microfluidics research in blood filtration is very active but to date nobody has validated a low cost device that simultaneously filtrates small samples of whole blood and reproducibly recovers clinically relevant biomarkers, and all this in a limited amount of time with undiluted raw samples. In this paper, we show first that plasma filtration from undiluted whole blood is feasible and reproducible in a low-cost microfluidic device. This novel microfluidic blood filtration element (BFE) extracts 12 μl of plasma from 100 μl of whole blood in less than 10 min. Then, we demonstrate that our device is valid for clinical studies by measuring the adsorption of interleukins through our system. This adsorption is reproducible for interleukins IL6, IL8, and IL10 but not for TNFα. Hence, our BFE is valid for clinical diagnostics with simple calibration prior to performing any measurement.  相似文献   

Recruiting and counseling people of color at Western universities     

Robbie W. Christler Tourse 《International journal for the advancement of counseling》1987,10(1):45-58

This article focuses on the recruitment and retention of students of color in Western universities. It highlights concepts and strategies which have been effective in recruiting and counseling students who are racial minorities. The content is based on the author's experience in practice over the past six years and is supported by literature, empirical data, and interviews. Vignettes related to work done with students of color are incorporated within the body of the paper.Although the article is derived from work the author has performed at an American university, the implications and conclusions of that work should be of interest to all Western institutions of higher learning that recruit and want to retain students of color in their respective academic environments.  相似文献   

Psychotherapy and chronic hemodialysis     

D. Robbie Campbell  Birendra K. Sinha 《International journal for the advancement of counseling》1983,6(1):47-60

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Exploring Curriculum in the Field: An Alternative to Institutionalised Learning     

Alison Elliott  >Robbie Lawson 《Asia-Pacific Journal of Teacher Education》1985,13(1):63-71

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HPT in the classroom: Practical applications     

Richard F. Gerson  Robbie G. Gerson 《Performance Improvement》2008,47(2):41-45

Human performance technology (HPT) is an area of professional practice most often found in the business world rather than in education. As adjunct professors at several universities, we have incorporated the principles of HPT into our classes. This article describes some of the courses where we have used aspects and models of HPT to teach students about subject areas in business. In addition, an entire graduate program based on HPT has been started at ITSON University in Mexico.  相似文献   

The learning organization: Concepts,processes, and questions     

Tracy Greenwood  Autar Wasson  Robbie Giles 《Performance Improvement》1993,32(4):7-11

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Association of Gestational Diabetes Mellitus and Perinatal Maternal Depression with Early Childhood Behavioral Problems: An Environmental Influences on Child Health Outcomes (ECHO) Study     

Lauren C. Shuffrey  Santiago Morales  Melanie H. Jacobson  Michelle Bosquet Enlow  Akhgar Ghassabian  Amy E. Margolis  Maristella Lucchini  Kecia N. Carroll  Rosa M. Crum  Dana Dabelea  Arielle Deutsch  William P. Fifer  Brandon Goldson  Christine W. Hockett  W. Alex Mason  Lisette T. Jacobson  Thomas G. O'Connor  Nicolò Pini  Yael Rayport  Ayesha Sania  Leonardo Trasande  Rosalind J. Wright  Seonjoo Lee  Catherine Monk  program collaborators for Environmental influences on Child Health Outcomes 《Child development》2023,94(6):1595-1609

This study examined the association of gestational diabetes mellitus (GDM), prenatal, and postnatal maternal depressive symptoms with externalizing, internalizing, and autism spectrum problems on the Preschool Child Behavior Checklist in 2379 children aged 4.12 ± 0.60 (48% female; 47% White, 32% Black, 15% Mixed Race, 4% Asian, <2% American Indian/Alaskan Native, <2% Native Hawaiian; 23% Hispanic). Data were collected from the NIH Environmental influences on Child Health Outcomes (ECHO) Program from 2009-2021. GDM, prenatal, and postnatal maternal depressive symptoms were each associated with increased child externalizing and internalizing problems. GDM was associated with increased autism behaviors only among children exposed to perinatal maternal depressive symptoms above the median level. Stratified analyses revealed a relation between GDM and child outcomes in males only.  相似文献