High-throughput study of alpha-synuclein expression in yeast using microfluidics for control of local cellular microenvironment   总被引：1，自引：0，他引：1  

Rosa P  Tenreiro S  Chu V  Outeiro TF  Conde JP 《Biomicrofluidics》2012,6(1):14109-141099

Microfluidics is an emerging technology which allows the miniaturization, integration, and automation of fluid handling processes. Microfluidic systems offer low sample consumption, significantly reduced processing time, and the prospect of massive parallelization. A microfluidic platform was developed for the control of the soluble cellular microenvironment of Saccharomyces cerevisiae cells, which enabled high-throughput monitoring of the controlled expression of alpha-synuclein (aSyn), a protein involved in Parkinson's disease. Y-shaped structures were fabricated using particle desorption mass spectrometry-based soft-lithography techniques to generate biomolecular gradients along a microchannel. Cell traps integrated along the microchannel allowed the positioning and monitoring of cells in precise locations, where different, well-controlled chemical environments were established. S. cerevisiae cells genetically engineered to encode the fusion protein aSyn-GFP (green fluorescent protein) under the control of GAL1, a galactose inducible promoter, were loaded in the microfluidic structure. A galactose concentration gradient was established in the channel and a time-dependent aSyn-GFP expression was obtained as a function of the positioning of cells along the galactose gradient. Our results demonstrate the applicability of this microfluidic platform to the spatiotemporal control of cellular microenvironment and open a range of possibilities for the study of cellular processes based on single-cell analysis.  相似文献   

Acculturation, well-being and classroom behaviour among white British and British Asian primary-school children in the south–east of England: Validating a child-friendly measure of acculturation attitudes     

Dennis Nigbur  Rupert Brown  Lindsey Cameron  Rosa Hossain  Anick Landau  Dominique Le Touze  Adam Rutland  Charles Watters 《Int J Intercult Relat》2008,32(6):493-504

Using a sample of white British and British Asian primary-school children (N = 386, aged 5–11 years), we measured acculturation attitudes (own and perceived outgroup), correlated constructs (ingroup and outgroup affect and identification) and relevant outcomes (self-esteem, classroom demeanour) in a structured interview to validate a customised, child-friendly measure of acculturation attitudes based on Berry's framework. Scale items measuring desire for culture maintenance and intergroup contact loaded onto the predicted factors, were internally reliable and showed concurrent validity with affect and identification. The predictive utility of measures was demonstrated in associations between children's acculturation attitudes (or perceived discrepancies with those of the outgroup) and outcomes such as self-esteem and teacher ratings of emotional symptoms.  相似文献   

The communication flow of research projects results     

Daniela Luzi  Maria Castriotta  Rosa Di Cesare  Luciana Libutti  Mariaosaria Manco 《Publishing Research Quarterly》2004,20(1):13-24

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Theory,construction, and use of the photometric integrating sphere     

E.B. Rosa  A.H. Taylor 《Journal of The Franklin Institute》1923,195(1):107-109

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Homework and academic achievement across Spanish Compulsory Education     

José Carlos Núñez  Natalia Suárez  Rebeca Cerezo  Julio González-Pienda  Pedro Rosário  Rosa Mourão 《教育心理学》2015,35(6):726-746

Homework (HW) is one of the most common school-related activities among parents, students and teachers. However, the need to assign HW to students has been extensively debated. The present investigation examines the relationship between specific HW variables (i.e. amount of HW completed, time spent on HW and perceived quality of HW time management) and academic achievement, while controlling for students’ gender and grade level. Participants included 454 students (ranging from 10 to 16?years of age) from three schools in northern Spain. A multivariate analyses of variance and path analyses showed that the amount of HW completed decreased with increased schooling, as did students’ perceived quality of HW time management. Data from hierarchical regression analyses completed by path analyses provided evidence that time spent on HW conjointly with perceived quality of HW time management explains how academic achievement is mediated by the amount of HW completed. These findings deepen the understanding of the complex impact of time spent on HW and on academic achievement.  相似文献   

Social presence and virtual collaborative learning processes in higher education / Presencia social y procesos de aprendizaje colaborativo virtual en educación superior     

Rosa Colomina  Ana Remesal 《Infancia y Aprendizaje》2015,38(3):647-680

The concept of social presence proposed in studies on communities of inquiry has highlighted both the importance and the challenges of integrating aspects such as affective and relational expressions among team members while solving collaborative learning tasks, in order to advance in a global comprehension of learning processes. This paper presents a theoretical and methodological proposal for tackling this issue from a socio-cultural perspective by analysing the participants’ discourse in relation to expressions of self-competence (at an individual and group level) concerning the task and group cohesion (task cohesion and social cohesion). This proposal’s potential is illustrated by the empirical results of a case study that analyses social presence during a collaborative work task by university students using the Moodle learning platform.  相似文献   

Development of a three-dimensional cell culture system based on microfluidics for nuclear magnetic resonance and optical monitoring     

Vicent Esteve  Javier Berganzo  Rosa Monge  M. Carmen Martínez-Bisbal  Rosa Villa  Bernardo Celda  Luis Fernandez 《Biomicrofluidics》2014,8(6)

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Complements or substitutes? The role of universities and local context in supporting the creation of academic spin-offs     

Riccardo Fini  Rosa Grimaldi  Simone Santoni  Maurizio Sobrero 《Research Policy》2011,40(8):1113-1127

In this paper, we analyze the extent to which University-Level Support Mechanisms (ULSMs) and Local-Context Support Mechanisms (LCSMs) complement or substitute for each other in fostering the creation of academic spin-offs. Using a sample of 404 companies spun off from the 64 Italian Science, Technology, Engineering, and Mathematics universities (STEM universities) over the 2000–2007 period, we show that the ULSMs’ marginal effect on universities’ spin-off productivity may be positive or negative depending on the contribution offered by different LCSMs. Specifically, in any given region, ULSMs complement the legislative support offered to high-tech entrepreneurship whereas they have a substitution effect with regard to the amount of regional social capital, regional financial development, the presence of a regional business incubator, regional public R&D expenses as well as the level of innovative performance in the region. Results support the idea that regional settings’ idiosyncrasies should be considered for universities to develop effective spin-off support policies. This paper contributes to the debate on the evaluation of economic policies supporting entrepreneurship.  相似文献   

Stimulating perceptions of kingship: royal imagery in the Cathedral of Monreale and in the Church of Santa Maria dell'Ammiraglio in Palermo1     

Rosa Bacile 《Al-Masaq: Islam & the Medieval Mediterranean》2004,16(1):17-52

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Perspectives of specific language disability     

Hagin  Rosa A. 《Annals of dyslexia》1971,21(1):22-34

Annals of Dyslexia -  相似文献