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111.
Focusing suspended particles in a fluid into a single file is often necessary prior to continuous-flow detection, analysis, and separation. Electrokinetic particle focusing has been demonstrated in constricted microchannels by the use of the constriction-induced dielectrophoresis. However, previous studies on this subject have been limited to Newtonian fluids only. We report in this paper an experimental investigation of the viscoelastic effects on electrokinetic particle focusing in non-Newtonian polyethylene oxide solutions through a constricted microchannel. The width of the focused particle stream is found NOT to decrease with the increase in DC electric field, which is different from that in Newtonian fluids. Moreover, particle aggregations are observed at relatively high electric fields to first form inside the constriction. They can then either move forward and exit the constriction in an explosive mode or roll back to the constriction entrance for further accumulations. These unexpected phenomena are distinct from the findings in our earlier paper [Lu et al., Biomicrofluidics 8, 021802 (2014)], where particles are observed to oscillate inside the constriction and not to pass through until a chain of sufficient length is formed. They are speculated to be a consequence of the fluid viscoelasticity effects. 相似文献
112.
To analyze the jamming and unjamming transition of oil-in-water emulsions under continuous temperature change, we simulated an emulsion system whose critical volume fraction was 0.3, which was validated with experimental results under oscillatory shear stress. In addition, we calculated the elastic modulus using the phase lag between strain and stress. Through heating and cooling, the emulsion experienced unjamming and jamming. A phenomenon—which is when the elastic modulus does not reach the expected value at the isothermal system—occurred when the emulsion system was cooled. We determined that this phenomenon was caused by the frequency being faster than the relaxation of the deformed droplets. We justified the relation between the frequency and relaxation by simulating the frequency dependency of the difference between the elastic modulus when cooled and the expected value at the same temperature. 相似文献
113.
This study compared views on homosexuality among US adults who varied in their consumption of pornography. Nationally representative cross-sectional data generated by the General Social Survey (GSS) between 2000 and 2012 were employed. Pornography consumers expressed more positive attitudes toward gay individuals' civil liberties, more moral acceptance of homosexuality, and more support for same-sex marriage. Moderation analyses indicated the importance of consumers' views on personal freedom and morality. Associations between pornography consumption and positive views on homosexuality were strongest when consumers placed a premium on personal freedom and adopted a relativistic perspective on morality. Mediation analyses indicated that pornography consumption indirectly predicted more positive views on homosexuality through a nontraditional attitude toward sex. Supplementary analysis of nationally representative three-wave GSS panel data confirmed the temporal sequencing of these links. Prior pornography consumption predicted a more positive subsequent attitude toward nontraditional sex, which in turn predicted more positive subsequent views on homosexuality. 相似文献
114.
桑琰云 《图书馆工作与研究》2014,(10)
本文将网络嵌入性理论运用于咨询联盟网络中.从联盟网络的社会性、联盟资源的异质性和联盟网络的互惠学习性三个方面分析了嵌入性时咨询联盟研究的启示,指出咨询联盟网络中不同社会资本的嵌入、认同的嵌入、关系的嵌入、文化的嵌入会带来网络的平衡发展,认为鼓励馆员开通自己的虚拟社区、建立连锁董事关系网络、发展边界人、避免文化的“过嵌入”这些措施会全面发挥网络嵌入所带来连锁效应,指引咨询联盟网络服务创新发展. 相似文献
115.
John DuBose Xinyu Lu Saurin Patel Shizhi Qian Sang Woo Joo Xiangchun Xuan 《Biomicrofluidics》2014,8(1)
Shape is an intrinsic marker of cell cycle, an important factor for identifying a bioparticle, and also a useful indicator of cell state for disease diagnostics. Therefore, shape can be a specific marker in label-free particle and cell separation for various chemical and biological applications. We demonstrate in this work a continuous-flow electrical sorting of spherical and peanut-shaped particles of similar volumes in an asymmetric double-spiral microchannel. It exploits curvature-induced dielectrophoresis to focus particles to a tight stream in the first spiral without any sheath flow and subsequently displace them to shape-dependent flow paths in the second spiral without any external force. We also develop a numerical model to simulate and understand this shape-based particle sorting in spiral microchannels. The predicted particle trajectories agree qualitatively with the experimental observation. 相似文献
116.
117.
The aim of this study was to quantify the physiological loads of programmed "pre-season" and "in-season" training in professional soccer players. Data for players during each period were included for analysis (pre-season, n = 12; in-season, n = 10). We monitored physiological loading of training by measuring heart rate and rating of perceived exertion (RPE). Training loads were calculated by multiplying RPE score by the duration of training sessions. Each session was sub-categorized as physical, technical/tactical, physical and technical/tactical training. Average physiological loads in pre-season (heart rate 124 ± 7 beats · min(-1); training load 4343 ± 329 Borg scale · min) were higher compared with in-season (heart rate 112 ± 7 beats · min(-1); training load 1703 ± 173 Borg scale · min) (P < 0.05) and there was a greater proportion of time spent in 80-100% maximum heart rate zones (18 ± 2 vs. 5 ± 2%; P < 0.05). Such differences appear attributable to the higher intensities in technical/tactical sessions during pre-season (pre-season: heart rate 137 ± 8 beats · min(-1); training load 321 ± 23 Borg scale · min; in-season: heart rate 114 ± 9 beats · min(-1); training load 174 ± 27 Borg scale · min; P < 0.05). These findings demonstrate that pre-season training is more intense than in-season training. Such data indicate that these adjustments in load are a direct attempt to deliver training to promote specific training adaptations. 相似文献
118.
A microfluidic device integrated with 3D thin film microelectrode arrays wrapped around serpentine-shaped microchannel walls has been designed, fabricated and tested for cell electrofusion. Each microelectrode array has 1015 discrete microelectrodes patterned on each side wall, and the adjacent microelectrodes are separated by coplanar dielectric channel wall. The device was tested to electrofuse K562 cells under a relatively low voltage. Under an AC electric field applied between the pair of the microelectrode arrays, cells are paired at the edge of each discrete microelectrode due to the induced positive dielectrophoresis. Subsequently, electric pulse signals are sequentially applied between the microelectrode arrays to induce electroporation and electrofusion. Compared to the design with thin film microelectrode arrays deposited at the bottom of the side walls, the 3D thin film microelectrode array could induce electroporation and electrofusion under a lower voltage. The staggered electrode arrays on opposing side walls induce inhomogeneous electric field distribution, which could avoid multi-cell fusion. The alignment and pairing efficiencies of K562 cells in this device were 99% and 70.7%, respectively. The electric pulse of low voltage (~9 V) could induce electrofusion of these cells, and the fusion efficiency was about 43.1% of total cells loaded into the device, which is much higher than that of the convectional and most existing microfluidics-based electrofusion devices. 相似文献
119.
120.
我国民航强国目标的实现必须依靠飞行技术人才素质的提高,民航高校是培养民用航空初始飞行人才的重要基地。本文试就民航高校飞行学员思想政治与职业道德素养、信息素质与教育理念培育、思维品质与思维能力养成、身体与心理素养、民航英语教学以及专业教师地位与素质提高等方面提出一些粗浅的看法,以促进未来民航飞行学员综合素质的提升。 相似文献