Development of SMEs and heterogeneity of trajectories: the case of biotechnology in France   总被引：1，自引：0，他引：1  

Vincent Mangematin  Stéphane LemariéJean-Pierre Boissin  David CatherineFrédéric Corolleur  Roger CoroniniMichel Trommetter 《Research Policy》2003,32(4):621-638

Biotechnology is an emergent sector based on the creation of research-intensive Small and Medium Enterprises (SMEs). While some SMEs are growing, most of them remain small, even those set-up several years ago. What is the pattern of development of the biotech sector? What are the patterns of development of firms? Studies on the development of high-tech SMEs have focused on a business model, in which entrepreneurs rely on growth forecasts to persuade capital investors (business angels and venture capitalists) to invest in a radical innovation project. Firms aim for a world market to industrialise their innovation, and initial public offering (IPO) enables initial investors to make profits that offset risky initial investment. While this model is appealing, it is simply one of the possible models of biotechnology development. Some firms are not designed to experience exponential growth, and choose to target local markets. Moreover, not all firms have the ambition of being listed on the stock exchange. Based on an in-depth analysis of the business and development of 60 French biotech SMEs, this article identifies two business models. By defining the development trajectories of each of these models, it highlights the temporary nature of the emergent model.  相似文献   

DOES THE SCHOOL COMPOSITION EFFECT MATTER? EVIDENCE FROM BELGIAN DATA   总被引：2，自引：1，他引：1  

Xavier  Dumay  Vincent  Dupriez 《British Journal of Educational Studies》2008,56(4):440-477

ABSTRACT:  Even if the literature on the effects of pupil composition has been extensive, no clear consensus has been reached concerning the significance and magnitude of this effect. The first objective of this article is to estimate the magnitude of the school composition effect in primary schools (6th grade) in French-speaking Belgium. Different indicators of school composition are used: academic, socio-cultural, 'language' and sex composition. Except for sex composition, the results show that the school composition effect explains significant amount of between schools variance even after controlling for pupils' initial performance, socio-cultural background, and non-cognitive dispositions. The second objective is to examine covariance between school composition and several organisational variables and their joint effect on school performance. The second set of analyses is intended to question the conceptual nature of the school composition effect, establishing whether it is direct or indirect.  相似文献   

Regional inequality in reading performance: an exploration in Belgium     

Bo Ning  Jan Van Damme  Wim Van Den Noortgate  Sarah Gielen  Kim Bellens  Vincent Dupriez 《School Effectiveness & School Improvement》2016,27(4):642-668

In the 2009 Programme for International Student Assessment, the Flemish community of Belgium outscored its French community in reading, with low achievers accounting for a large proportion of the score gaps. In this study, between-community comparisons based on the Blinder-Oaxaca decomposition method showed that the Flemish community benefits largely from its policies and practices of giving schools relative autonomy in resource allocation, promoting good student conduct, and decreasing grade retention. Between-community comparisons using the Juhn-Murphy-Pierce decomposition method showed that compared to their Flemish peers in the same percentile, low achievers in the French community are hindered more by their relatively low grade level; disadvantageous school economic, social, and cultural composition; and negative school supportive climate while medium and high achievers benefit more from their advantageous school economic, social, and cultural composition; positive supportive climate; and high proportion of qualified teachers in schools.  相似文献   

Antiviral activities of extracts from Hong Kong seaweeds     

Engchoon Vincent OOI  Put O. ANG Jr. 《Journal of Zhejiang University. Science. B》2008,9(12):969-976

We extracted six Hong Kong brown seaweed species with hot water for their antiviral properties. The cytotoxicity and antiviral activity of these extracts were tested by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenlytetrezolium bromide] method, cytopathic effect reduction assay, and plaque reduction assay. The antiviral effect was further determined by flow cytometric analysis. The results showed that most of these extracts inhibited the propagation of herpes simplex virus types 1 and 2 (HSV-1 and HSV-2) standard strains with very low cytotoxicity to the host cells. The extracts ofHydroclathrus clathratus and Lobophora variegata showed more potential anti-HSV activities than the extracts of the other four seaweeds. They also had moderate anti-respiratory syncytial virus (RSV) activities but could not inhibit influenza A virus. Hydroclathrus clathratus was further extracted by diluted acid and alkali and the antiviral effects of the extracts were also detected. The result showed that the hot water extract contained the main carbohydrate components that exhibited the antiviral activities against various strains of HSV, including the acyclovir-resistant strain. HI-3, a compound fractionated from this hot water extract, showed a dose-dependent anti-HSV activity in flow cytometric analysis and plaque reduction assay.  相似文献   

生命安全教育：体育教育的本源回归探索     

刘德  王华倬 《北京体育大学学报》2015,38(7):112-116+127

摘要：生命安全教育是现代教育“以人为本”的核心价值体现和素质教育的重要内容。采用文献资料法、逻辑分析法,以体育的本源为逻辑起点和研究对象,从生命安全教育的视角审视体育产生过程和体育起源学说。旨在探索原始体育的价值取向和隐性功能,进而分析生命安全教育在体育教育中的本源地位和作用,阐释体育教育中的生命安全教育品性,体现个体生命发展的安全需求,以期回归体育教育的本真。其研究价值在于为学校体育中生命安全教育的课程建设提供理论依据。结论：体育的产生、形成和发展过程带有明显的生存技能及安全教育特征;生命安全教育是体育的本源要素;进入21世纪的现代文明社会,生命安全教育在学校体育教育中具有重要的价值和功能。  相似文献   

Differential effects of a long teacher training internship on students’ learning-to-teach patterns     

Vincent Donche  Maaike D. Endedijk  Tine van Daal 《欧洲师范教育杂志》2015,38(4):484-495

To become a lifelong learner as a teacher, student teachers already have to learn how to direct their own learning during initial teacher education programmes. Previous empirical research has shown that student teachers differ in their patterns of learning-to-teach, but few is known about the changeability of these learning patterns throughout teacher education and the role of teacher training internships in this. In this study, the changes in student teachers’ patterns of learning-to-teach amongst pre-service teachers were investigated using a longitudinal design. 253 student teachers were asked to complete the ‘Inventory Learning To Teach Process’ questionnaire at two points during the last semester of a three-year teaching programme: immediately prior to and immediately following a long teacher training internship. The results of this study demonstrate that learning-to-teach patterns are subject to a relative degree of change. In particular, survival-oriented student teachers appear to have undergone a greater degree of change after the long teacher training internship.  相似文献   

Visualisation to support children with attention-deficit/hyperactivity disorder learning to solve mathematical word problems: A randomised controlled trial     

Fatemah Almuwaiziri  Natthapoj Vincent Trakulphadetkrai  Timothy Williams 《British Journal of Special Education》2023,50(2):314-324

This randomised controlled trial experimental study compared the effectiveness of using two different types of visualisation – self-constructed visualisation (SCV) and passively received visualisation (PRV) – to help children with attention-deficit/hyperactivity disorder (ADHD) solve mathematical word problems. While SCV refers to drawings that children create to help solve given word problems, PRV refers to pre-made images that accompany word problems. Twenty children with ADHD in Kuwait, aged nine to 11 years, were randomly assigned to either the SCV or PRV group, where they were taught to use either SCV or PRV to solve word problems across 20 daily one-to-one sessions. The results showed that regardless of the visualisation type, children's word problem-solving ability significantly improved. Children with ADHD should thus be encouraged to use visualisation to help make the word problem-solving process more accessible to them.  相似文献   

Learning pattern development throughout higher education: A longitudinal study     

Vincent Donche  Liesje Coertjens  Peter Van Petegem 《Learning and individual differences》2010,20(3):256-259

Research on learning pattern development in higher education contexts is scarce. This longitudinal study seeks to address this issue by analysing the development of students' learning patterns throughout University College. Vermunts' Inventory of Learning Styles was used to assess individual differences in learning conceptions and learning strategies. By examining intra-individual changes in learning patterns we expected to find developmental trends within learning patterns. Results show that meaning oriented learning increases over time and undirected learning decreases. Some learning patterns are however more subject to change than others. The development of learning patterns was found to be relative and dependent on the learning pattern which students have already acquired in the first-year of University College.  相似文献   

Power,performance, and expectations in the dismissal of NBA coaches: A survival analysis study     

David B. Wangrow  Donald J. Schepker  Vincent L. Barker 《Sport Management Review》2018,21(4):333-346

Researchers focusing on coach dismissal often examine organizational performance as an antecedent; however, there has been minimal attention to the ways in which countervailing dimensions of power associated with coaches and ownership influence dismissal decisions, while still considering performance as well as expectations. To understand how power, performance and expectations affect coach dismissals, the authors examine the tenures of coaches in the North American-based National Basketball Association and use survival analysis to test a model of coach dismissals using 31 years of observations. The authors find that coach dismissal is a process influenced not only by team performance, but also expectations and a coach’s power that accrues from both structure and prestige. Additional findings suggest that expert power influences the likelihood of coach dismissal, but only after the coach has been in the job for several years. Finally, the authors found a non-linear relationship between performance and the probability of coach dismissal, suggesting that single events (i.e., wins or losses) have less influence on the probability of dismissal when performance is very poor or above average.  相似文献   

Enhancement of biosensing performance in a droplet-based bioreactor by in situ microstreaming     

Olivier Ducloux  Elisabeth Galopin  Farzam Zoueshtiagh  Alain Merlen    Vincent Thomy 《Biomicrofluidics》2010,4(1)

A droplet-based micro-total-analysis system involving biosensor performance enhancement by integrated surface-acoustic-wave (SAW) microstreaming is shown. The bioreactor consists of an encapsulated droplet with a biosensor on its periphery, with in situ streaming induced by SAW. This paper highlights the characterization by particle image tracking of the speed distribution inside the droplet. The analyte-biosensor interaction is then evaluated by finite element simulation with different streaming conditions. Calculation of the biosensing enhancement shows an optimum in the biosensor response. These results confirm that the evaluation of the Damköhler and Peclet numbers is of primary importance when designing biosensors enhanced by streaming.It has been pointed out that biosensing performances can be limited by the diffusion of the analytes near the sensing surface.¹ In the case of low Peclet number hydrodynamic flows, typical of microfluidic systems, molecule displacements are mainly governed by diffusive effects that affect time scales and sensitivity. To overcome this problem, the enhancement of biosensor performance by electrothermal stirring within microchannels was first reported by Meinhart et al.² Other authors^{3, 4} numerically studied the analyte transport as a function of the position of a nanowire-based sensor inside a microchannel, stressing on the fact that the challenge for nanobiosensors is not the sensor itself but the fluidic system that delivers the sample. Addressing this problem, Squires et al.⁵ developed a simple model applicable to biosensors embedded in microchannels. However, the presented model is limited to the case of a steady flow. The use of surface-acoustic waves (SAWs) for stirring in biomicrofluidic and chemical systems is becoming a popular investigation field,^{6, 7, 8, 9} especially to overcome problems linked to steady flows by enhancing the liquid∕surface interaction.^{1, 10, 11} The main challenges that need to be addressed when using SAW-induced stirring are the complexity of the flow and its poor reproducibility. However, some technical solutions were proposed to yield a simplified microstreaming. Yeo et al. presented a centrifugation system based on SAW that produces the rotation of the liquid in a droplet in a reproducible way by playing on the configuration of the transducers and reflectors,¹² and presented a comprehensive experimental study of the three-dimensional (3D) flow that causes particle concentration in SAW-stirred droplets,¹³ revealing the presence of an azimuthal secondary flow in addition to the main vortexlike circular flow present in acoustically stirred droplets. The efficiency of SAW stirring in microdroplets to favorably cope with mass transport issues was finally shown by Galopin et al.,¹⁴ but the effect of the stirring on the analyte∕biosensor interaction was not studied. It is expected to overcome mass transport limitations by bringing fresh analytes from the bulk solution to the sensing surface.The studied system, described in Fig. ​Fig.1,1, consists of a microliter droplet microchamber squeezed between a hydrophobic piezoelectric substrate and a hydrophobic glass cover. Rayleigh SAWs are generated using interdigitated transducers (interdigital spacing of 50 μm) laid on an X-cut LiNbO₃ substrate.^{1, 15, 16} The hydrophobicity of the substrate and the cover are obtained by grafting octadecyltrichlorosilane (OTS) self-assembled monolayers (contact angle of 108° and hysteresis of 9°). To do so, the surface is first hydroxylized using oxygen plasma (150 W, 100 mT, and 30 sccm³ O₂) during 1 min and then immersed for 3 h into a 1 mM OTS solution with n-hexane as a solvent.Open in a separate windowFigure 1(a) General view of the considered system. (b) Mean value of the measured speeds within the droplet as a function of the inlet power before amplification.When Rayleigh waves are radiated toward one-half of the microchamber, a vortex is created in the liquid around an axis orthogonal to the substrate due to the momentum transfer between the solid and the liquid. This wave is generated under the Rayleigh angle into the liquid.Speed cartographies of the flow induced in the droplet are realized using the particle image tracking technique for different SAW generation powers. To do so, instantaneous images of the flow are taken with a high-speed video camera at 200 frames∕s and an aperture time of 500 μs on a 0.25 μl droplet containing 1 μm diameter fluorescent particles. Figure ​Figure11 shows the mean speed measured in the droplet as a function of the inlet power. The great dependence of the induced mean speed with the SAW power enables a large range of flow speeds in the stirred droplet. Moreover, the flow was visualized with a low depth of field objective. It was found to be circular and two dimensional (2D) in a large thickness range of the droplet.The binding of analytes to immobilized ligands on a biosensor is a two step process, including the mass transport of the analyte to the surface, followed by a complexation step,Abulk↔kmAsurface+B↔ka,kdAB(1)with k_m as the constant rate for mass transport from and to the sensor, and k_a and k_d as the constant rates of association and dissociation of the complex.At the biosensor surface, the reaction kinetics consumes analytes but their transport is limited by diffusive effects. In this case, the Damköhler number brings valuable information by comparing these two effects. Calling the characteristic time of reaction and diffusion, respectively, τ_C and τ_M, the mixing time in diffusion regime can be approximated by τ_M≈h²∕D with D as the diffusion coefficient and h a characteristic length of the microchannel. Calling R_T the ligand concentration on the surface in mole∕m², the Damköhler number (D_a) can be written asDa=τMτC=kaRThD.(2)Depending on the type of reaction, the calculation of D_a helps determine if a specific biointeraction will benefit from a mass SAW-based microstreaming. If the Damköhler number is low, the reaction is slow compared to mass transport and the reaction will not significantly benefit from microstirring. For example, the hybridization of 19 base single stranded DNA in a microfluidic system with a characteristic length of 500 μm is characterized by a Damköhler number of 0.07 and is therefore not significantly influenced by mass transport. On the contrary, the binding of biotin to immobilized streptavidin is characterized by a D_a number of approximately 10⁴. In this case, the stirring solution will significantly improve the reaction rate.COMSOL numerical simulations were carried out to study the efficiency of the SAW stirring in the case of a droplet-based microbioreactor with a diameter of 1 mm. Assuming a 2D flow, the simulated model takes into account the convective and diffusive effects in the analyte-carrying fluid and the binding kinetics on the biosensor surface. This approach was thoroughly developed by Meinhart et al.²On the biosensor surface, the following equations are solved:∂B∂t=kacs(RT−B)−kdB,(3)∂B∂t=D|∂c∂y|y=0(4)with c as the local concentration of analytes in the droplet and B as the surface concentration of bound analytes on the biosensor surface. Simulation results show that a depleted zone is formed near the biosensor in the case of an interaction without stirring. This zone is characterized by a low concentration of analytes and results from the trapping of analytes on the biosensor surface, thus creating a concentration gradient on the vicinity of the biosensor. When stirring is applied, the geometry of the depleted zone is modified, as it is pushed in the direction of the flow. The geometry of the depleted zone then depends on many parameters, among which the diffusion coefficient D, the speed distribution of the flow (not only near the biosensor but also in the whole microfluidic system), and the reaction kinetics on the biosensor. In our case, which is assimilated to a simple circular flow, the depleted zone reaches a permanent state consisting of an analyte-poor layer situated in the exterior perimeter of the stirred droplet. The diffusion of analytes is then limited again by diffusion from the inner part of the droplet toward its exterior perimeter (see Fig. ​Fig.22).Open in a separate windowFigure 2(a) Mean concentration of bound analytes vs time for different mean flow speeds. (b) The obtained concentration profiles with and without circular stirring, t=10 000 s.The initial analyte and receptor concentrations are, respectively, 0.1 nM in the solution and 3.3×10⁻³ nM m on the biosensor surface, the diffusion coefficient is D=10⁻¹¹ m² s⁻¹, and the reaction constants are k_a=10⁶ M⁻¹ s⁻¹ and k_d=10⁻³ s⁻¹. Simulations show that the mean concentration of bound analytes highly increases with the flow speed, improving the efficiency of the biosensing device. To evaluate the benefits of in situ microstreaming with SAW, the same simulations were conducted for D_a numbers ranging from 10⁴ to 10⁸ M⁻¹∕s, by ranging the diffusion coefficient from 4×10⁻¹² to 4×10⁻⁹ m²∕s, and the association coefficient k_a from 10⁴ to 10⁸ M⁻¹∕s. The enhancement factor of analyte capture, defined as the ratio of the binding rate with streaming B and the binding rate without streaming B0, is plotted in Fig. ​Fig.33 for different values of D_a. Calculations are done in the case of a mean flow speed of 0.5 mm∕s.Open in a separate windowFigure 3(a) Enhancement factor (defined as the ratio between binding rate with streaming B and binding rate without streaming B0) for different Damkhöler numbers and (b) normalized enhancement factor for different Peclet numbers.One can notice the saturation of the enhancement factor curve for large value of D_a to the value of 3.5 for high D_a. This can be explained by the fact that for large k_a∕D_a ratios, the analytes, which normally require penetration in the depleted zone by diffusion, do not have time to interact with the biosensor when they pass in the vicinity of its surface. The efficiency of the streaming is then reduced for large values of D_a. In the case of our specific flow configuration, the enhancement factor reaches 3.2 for the interaction of streptavidin on immobilized biotin (D_a=10³).The reported simulation results can be compared to an experimental value obtained using the droplet-based surface plasmon resonance sensor streamed in situ using SAW reported by Yeo et al.¹² By monitoring the streptavidin∕biotin binding interaction on an activated gold slide, they showed that SAW stirring brings an improvement factor of more than 2. This difference can be accounted to the high complexity of the induced 3D flow, which was modeled in a simple manner in our calculations.Other factors must be taken into account when optimizing the improvement factor, such as the flow velocity and the characteristic length of the mixing. To do so, the Peclet number allows the comparison of the convective and diffusive effects.¹⁷ For δC a typical variation in concentration on the distance h, the Peclet number is given byPe=UhD.(5)A significantly high Peclet number causes a decrease in biosensing efficiency as the analytes do not have enough time to interact with the biosensing surface by diffusion through the analyte-poor layer. On the contrary, the case of a low Peclet number corresponds to the diffusion-limited problem. Therefore, for each Damköhler number, there is a Peclet number optimizing this factor. To illustrate this fact, Fig. ​Fig.3b3b shows the calculation of the enhancement factor as a function of the Peclet number for a given D_a.In this paper, we showed that surface loading of typical analytes on a droplet-based biosensor can be highly increased by SAW microstirring. The system permits the enhancement of the biosensing performances by the continuous renewal of the analyte-carrying fluid near the sensing surface. Thanks to mean flow speeds measured up to 1800 μm∕s, the SAW microstreaming can be beneficial to the biosensing of a large range of analyte∕ligand interactions. In addition to the biosensing performance improvement, such a method can be easily integrated in micro-micro-total-analysis systems, which makes it a convenient tool for liquid handling in future biochips.  相似文献