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11.
Vitamin B12 being water soluble is excreted in the urine when administered in excess. The probability of finding an abnormally excess
serum concentration would be almost surreal. We report a peculiar clinical situation that may impact the vitamin B12 immunoassay on the Roche Elecsys 2010 due to excess analyte concentration. In separate episodes (Feb and June 2010), the
Biochemistry laboratory of a tertiary-care hospital, Kolkata, India, encountered two critically ill patients with background
chronic kidney disease (CKD), low urine output, and on cyanocoabalamin supplementation, who had serum vitamin B12 concentrations far exceeding expected values; even post dialysis. The B12 assays (pmol/l) were performed using electrochemiluminiscence immunoassay on Roche Elecsys 2010, the assay validity confirmed
by concomitant quality control runs. The immunoassays failed to deliver results, flagged with “signal level below limit”. Biotin therapy was ruled out as a possible interferent. In the first episode, re-assay of a repeat draw yielded same outcome;
outsourcing on Immulite provided concentration of >738 pmol/l. Serial dilution gave result of >29520 pmol/l on Elecsys 2010.
In the second, we gained from past experience. Vitamin B12 concentration >59040 pmol/l was conveyed to the treating nephrologist the very day. The B12 immunoassay on the Elecsys 2010 employs sequential incubation steps for competitive binding that is compromised in the event
of abnormally excess B12 concentration in patient sera akin to the prozone effect. This knowledge may be beneficial while assaying sera of CKD patients
to avoid financial loss due unnecessary repeats and delay in turnaround time. 相似文献
12.
In this work, we have investigated text readability in Bangla language. Text readability is an indicator of the suitability of a given document with respect to a target reader group. Therefore, text readability has huge impact on educational content preparation. The advances in the field of natural language processing have enabled the automatic identification of reading difficulty of texts and contributed in the design and development of suitable educational materials. In spite of the fact that, Bangla is one of the major languages in India and the official language of Bangladesh, the research of text readability in Bangla is still in its nascent stage. In this paper, we have presented computational models to determine the readability of Bangla text documents based on syntactic properties. Since Bangla is a digital resource poor language, therefore, we were required to develop a novel dataset suitable for automatic identification of text properties. Our initial experiments have shown that existing English readability metrics are inapplicable for Bangla. Accordingly, we have proceeded towards new models for analyzing text readability in Bangla. We have considered language specific syntactic features of Bangla text in this work. We have identified major structural contributors responsible for text comprehensibility and subsequently developed readability models for Bangla texts. We have used different machine-learning methods such as regression, support vector machines (SVM) and support vector regression (SVR) to achieve our aim. The performance of the individual models has been compared against one another. We have conducted detailed user survey for data preparation, identification of important structural parameters of texts and validation of our proposed models. The work posses further implications in the field of educational research and in matching text to readers. 相似文献
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14.
Qualitative analysis of unknown organic compounds is an essential part of the core curriculum for BSc and MSc students in chemistry. The functional group detection is a significant component of qualitative analysis. The present work describes the reason for the failure of Mulliken-Barker test for nitro compounds having carboxylic acid functionality and suggests a possible remedy. 相似文献
15.
Surupa Basu S. Chaudhuri M. Bhattacharyya T. K. Chatterjee S. Todi A. Majumdar 《Indian journal of clinical biochemistry : IJCB》2010,25(2):146-152
This study was conducted to evaluate whether microalbuminuria on admission and after 24 hrs of admission to intensive care
unit (ICU) predicts outcome as well as the Acute Physiology and Chronic Health Evaluation (APACHE) II severity illness score,
the current accepted method of doing so. The study was carried out in a 20 bed mixed medical-surgical ICU of a tertiary care
hospital. Of 525 consecutive adult patients with ICU stay of more than 24 hrs, 238 were included for the study. Patients with
pregnancy, menstruation, anuria, macroscopic hematuria, urinary tract infection, marked proteinuria due to renal and post-renal
structural diseases, were excluded. Spot urine samples were collected on admission to ICU and 24 hrs thereafter. Urine albumincreatinine
ratio (ACR) was measured on ICU admission (ACR1) and after 24 hrs (ACR2) and expressed in mg/g. Patient demographics were
noted on admission. For disease severity scoring, APACHE II scores were calculated. Each patient was followed up throughout
their ICU stay for a maximum of 28 days and the following outcome data were obtained: ICU length of stay and ICU mortality.
Of the 238 patients, 196 survived while 42 patients died in the ICU. Non-survivors had a significantly higher median ACR2
[162.7 mg/g (IQR 69.5–344.3)] in comparison to the survivors who had a median ACR2 = 54.4 mg/g (IQR 19.0–129.1) (P< 0.0001).
The median ACR1 [161.0 mg/g (IQR 29.0–369.3)] of non-survivors was higher than the median ACR1 [80.4 mg/g (IQR 35.1–167.6)]
of survivors but failed to reach statistical significance (P= 0.0948). In a receiver operating characteristic curve (ROC)
analysis, ACR2 emerged as the best indicator of mortality [(area under curve (AUC) of ACR2 = 0.71 > AUC (ACR1) =0.58 > AUC
(ΔACR) =0.55] similar to the currently used APACHE II scores (AUC = 0.78) (P=0.3). At a cutoff of 101 mg/g, ACR2 had a sensitivity
of 69%, specificity of 67%, positive predictive value of 31% and a negative predictive value of 91% for predicting mortality
in the critically ill patients. Absence of significant microalbuminuria at 24 hrs of ICU admission may help to predict survival
in the ICU. 相似文献
16.
Shedid SM Saada HN Eltahawy NA Hammad AS 《Indian journal of clinical biochemistry : IJCB》2018,33(3):314-321
Radiation induced brain damage is associated with impairment of mitochondrial functions, variations in the level of neurotransmitters, and oxidative stress. Mitochondrial function is closely linked to the level of neurotransmitters since the precursors are supplied by the Kreb’s cycle intermediates. The objective of this study was to evaluate the influence of pantothenic acid, an essential component in the synthesis of Coenzyme A (CoA), on the activity of the Krebs cycle enzymes, isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), and succinate dehydrogenase (SDH); the level of aspartic, glutamic and GABA; the activity of transaminases, and oxidative stress, in the cerebrum of γ-irradiated rats. Pantothenic acid (26 mg/Kg) was orally administered to the rats, 2 h after irradiation and during the following 5 days. Animals were sacrificed the 7th day post-irradiation. The exposure of male albino rats to γ-rays (5 Gy) has triggered oxidative stress notified by a significant elevation in the level of malondialdehyde (MDA), an end product of lipid peroxidation, associated to a significant decrease in the content of phospholipids, and the antioxidant compound glutathione (GSH). The activity of IDH, α-KGDH, and SDH, has significantly decreased, while the level of aspartic, glutamic and GABA has significantly increased. In parallel to these changes, the activity of alanine and aspartate transaminase has significantly increased, compared to their values in the control rats. Pantothenic acid treatment, has significantly attenuated oxidative stress; enhanced the activity of IDH, α-KGDH, and SDH; minimized the increase in the level of amino acids and the activity of transaminases, compared to their values in the cerebrum of irradiated rats. In conclusion, pantothenic acid could improve the level of neurotransmitters amino acids, which depends on the enzymatic activities of Krebs cycle and linked to oxidative stress. 相似文献
17.
P. K. Basu 《Resonance》2007,12(10):37-46
The increased use of optoelectronic devicessuch as TV screens and computer monitors in everyday life, calls for a material
on which integration of all circuits may be performed. Silicon, the most widely used material in electronics, has limited
application in optoelectronics due to its poor light emission property. In this article, the need for silicon photonics in
present day ICs, and communication, the reason for its inefficient light emission, and recent breakthrough in the form of
Si Raman laser are discussed. 相似文献
18.
Pranab S. Basu Ramdhan Majhi Sudip Chatterjee Sandip K Batabyal 《Indian journal of clinical biochemistry : IJCB》2000,15(2):119-123
A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody
(TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The
protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially
purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid
autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column.
The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of
both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic
representation of peak height along with their estimation in autoimmune thyroid disorders. 相似文献
19.
Pallab Basu Sabyasachi Som Nabendu Choudhuri Harendranath Das 《Indian journal of clinical biochemistry : IJCB》2008,23(4):361-364
A randomized case control hospital based study was conducted over 12 months time on 31 asphyxiated and 31 normal newborn to
see whether urinary uric acid can be used as a marker of perinatal asphyxia and can be correlated with the clinical diagnosis
by Apgar score. Uric acid and creatinine were estimated in spot urine within 24 hours after birth in both cases and controls.
A ratio between concentrations of uric acid to creatinine was estimated and compared between cases and controls. It was found
that the ratios were significantly higher in cases than controls (3.1± 1.3 vs 0.96± 0.54; P < 0.001) and among asphyxia patients,
a significant negative linear correlation was found between the uric acid to creatinine ratio and the Apgar score (r = −0.857,
P < 0.001). So urinary uric acid to creatinine ratio can be used as an additional non-invasive dispace, easy and at the same
time early biochemical marker of birth asphyxia which biochemically supports the clinical diagnosis and severity grading of
asphyxia by Apgar score. 相似文献
20.
Vibha Uppal Chittranjan Vij Gurdeep Kaur Bedi Anil Vij Basu Dev Banerjee 《Indian journal of clinical biochemistry : IJCB》2013,28(4):336-341
The study was planned to assess the prevalence of thyroid disorders in type 2 diabetes in North Indian population and to correlate the serum insulin and glycosylated haemoglobin levels with thyroid hormones. It is a case control study. One hundred and twenty patients of type 2 diabetes were included in the study along with 117 adults of the same age group and normal glucose levels as controls. All blood samples were taken from subjects who fasted for at least 12 h before the blood collection. Glycosylated hemoglobin was determined by ion exchange chromatography and serum insulin and thyroid hormones were assessed through enzyme linked immunosorbent assay. Fasting blood glucose and glycosylated haemoglobin levels were significantly higher in diabetics showing a poor glucose control. Serum tri-iodothyronine values were significantly lower in diabetics. There was a significant correlation between glycosylated haemoglobin and thyroid hormones. There was no correlation between serum insulin and thyroid hormones. 相似文献