Microfluidic three-dimensional hydrodynamic flow focusing for the rapid protein concentration analysis     

Sungmin Hong  Pei-Hsiang Tsou  Chao-Kai Chou  Hirohito Yamaguchi  Chin B. Su  Mien-Chie Hung  Jun Kameoka 《Biomicrofluidics》2012,6(2)

A simple microfluidic 3D hydrodynamic flow focusing device has been developed and demonstrated quantitative determinations of quantum dot 525 with antibody (QD525-antibody) and hemagglutinin epitope tagged MAX (HA-MAX) protein concentrations. This device had a step depth cross junction structure at a hydrodynamic flow focusing point at which the analyte stream was flowed into a main detection channel and pinched not only horizontally but also vertically by two sheath streams. As a result, a triangular cross-sectional flow profile of the analyte stream was formed and the laser was focused on the top of the triangular shaped analyte stream. Since the detection volume was smaller than the radius of laser spot, a photon burst histogram showed Gaussian distribution, which was necessary for the quantitative analysis of protein concentration. By using this approach, a linear concentration curve of QD525-antibody down to 10 pM was demonstrated. In addition, the concentration of HA-MAX protein in HEK293 cell lysate was determined as 0.283 ± 0.015 nM. This approach requires for only 1 min determining protein concentration. As the best of our knowledge, this is the first time to determinate protein concentration by using single molecule detection techniques.  相似文献   

DSP-based synchronous control of dual linear motors via Sugeno type fuzzy neural network compensator     

Po-Huan Chou  Chin-Sheng Chen  Faa-Jeng Lin 《Journal of The Franklin Institute》2012,349(3):792-812

A digital signal processor (DSP)-based complementary sliding mode control (CSMC) with Sugeno type fuzzy neural network (SFNN) compensator is proposed in this study for the synchronous control of a dual linear motors servo system installed in a gantry position stage. The dual linear motors servo system comprises two parallel permanent magnet linear synchronous motors (PMLSMs). The dynamics of the single-axis motion system with a lumped uncertainty which contains parameter variations, external disturbances and nonlinear friction force is briefly introduced first. Then, a CSMC is designed to guarantee the precision position tracking requirement in single-axis control for the dual linear motors. Moreover, to enhance the robustness to uncertainties and to eliminate the synchronous error of dual linear motors, the CSMC with a SFNN compensator is proposed where the SFNN compensator is designed mainly to compensate the synchronous error. Furthermore, to increase the control performance of the proposed intelligent control approach, a 32-bit floating-point DSP, TMS320VC33, is adopted for the implementation of the proposed CSMC and SFNN. In addition, some experimental results are illustrated to show the validity of the proposed control approach.  相似文献   

影响教育虚拟社区中学习动机激发因素的实证分析   总被引：1，自引：0，他引：1  

胡凡刚  仇秀娟  刘玮  张春霞 《电大教学》2013,(4):52-59

教育虚拟社区中的学习动机是学习者通过自我调节,使自身的内在要求与学习行为的外在诱因相协调,引起和维持学习者在教育虚拟社区中的学习活动,并激励、指引学习者的行为朝向一定学习目标的内部心理状态。在基于课程＂学习科学与技术＂的教育虚拟社区学习实践的基础上,实证分析了教育虚拟社区中学习动机激发的影响因素,通过描述性统计、回归分析、因子分析和聚类分析发现：教师因素、学习者因素、环境因素和文化因素是影响学习者在教育虚拟社区中学习动机激发的四大要素,学习者在教育虚拟社区中学习动机的激发有赖于以上各因素协同作用的发挥,由此提出了影响教育虚拟社区学习动机激发因素的分析模型。  相似文献   

图书馆自动化集成系统的评价标准     

丑楚 《高校图书馆工作》2005,25(2):45-47

本文从系统性能、系统的运行模式、数据库管理系统、数据的标准化等六个方面分析了图书馆自动化集成系统软件的评价标准。参考文献10。  相似文献   

中国语文教学的两难境地     

卢筹 《教育学术月刊》2002,(5):37-38

中国语文近半个世纪的怪事不少，有时是一分为二，变成文学和语文，有时又合二为一，变成单一的语文；有时强调阶级性，为政治服务，课本里充斥伟人语录，有时又强调与生产劳动相结合，教材基本不讲不学；有时重视工具性，把语文课上成大学的语法课，有时又重视人文性，把语文课上成美学欣赏课。局外人对此百思不得其解，莫名其妙，局内人其实也不明就里，一再听风是雨，左右摇摆。中国的语文到底怎么啦？确实令人费解。按理说，有五千年的文化史，十几亿人的语文实践，加上现代科学思维的理性分析，区区语文学科应该不至于如此折腾。但事实…  相似文献   

An implementation and outcome evaluation of a multicomponent sexuality education programme for high school students     

Nancy F. Berglas  Petra Jerman  Louise A. Rohrbach  Francisca Angulo-Olaiz  Chih-Ping Chou  Norman A. Constantine 《Sex education》2016,16(5):549-567

Numerous classroom-based interventions have aimed to improve sexual health outcomes for young people, yet few have shown strong, lasting effects. Ecological approaches that address multiple levels of a young person’s environment offer largely untapped potential to positively change sexual behaviour. This paper presents results of a cluster-randomised trial of a multicomponent sexuality education intervention that integrated a classroom curriculum, parent education workshops and materials, peer advocate programme, and sexual health services at 10 urban high schools. An implementation evaluation, employing quantitative and qualitative instruments, was conducted to examine whether the individual components of the intervention were delivered as planned and how they were received by the target populations. Multilevel modelling was used to evaluate the effect of the multicomponent intervention on outcomes at one-year follow-up. Results showed that the intervention components were successfully implemented with and well received by target populations. Students receiving the multicomponent intervention reported greater increases in the use of sexual health services (odds ratio [OR] = 1.73, 95% CI = 1.09–2.75) and the likelihood of carrying a condom (OR = 2.71, 95% CI = 1.44–5.09) relative to those receiving a control condition. No effects were found for other behaviours, possibly due to low prevalence of sexual activity in the sample and the small number of schools randomised.  相似文献   

Developing a web-based two-tier test for internet literacy     

Chien Chou  Chin-Chung Tsai  Pei-Shan Chan 《British journal of educational technology : journal of the Council for Educational Technology》2007,38(2):369-372

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Co-sleeping and the Importation of Picture Books About Bedtime     

Wan-Hsiang Chou 《Children‘s Literature in Education》2009,40(1):19-32

In mainstream Western cultures where bedtime means isolation and separation from adults, picture books about bedtime are cultural artifacts created especially for this transitional process of separation. In a culture such as Taiwan where children often sleep with their parents, siblings, or other caregivers until elementary school, the need for picture books about bedtime is an imported concept. The large number of translated Western bedtime books found in Taiwan becomes a rich area in which to explore the impact of imported ideas of childhood on Taiwanese picture book creation. This article takes a close look at the books created by Taiwanese authors with a bedtime theme and notes their culturally hybrid qualities.

Wan-Hsiang ChouEmail:

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Leveraging Enterprise System Technology for Curricular Innovation and Redesign     

Special Issue Guest Editors  
John R. Grandzol David Chou 《Decision Sciences Journal of Innovative Education》2010,8(1):1-3

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康德论道德教育方法     

任丑 《教育学报》2007,3(4):77-82

康德认为，纯粹德性是人类的本性，它要求出于义务来遵守，而且是趋向于善的唯一动机，因此是可教的。这就是对道德教育的方法如何可能的问题的回答。在此基础上，康德提出了他的道德教育方法：道德判断力的训练、敬重义务的训练、合道德性的道德教育程序的建构、伦理共同体的联合等。康德的这一思想，给我们进行道德教育以如下几个方面的启迪：注重道德教育方式本身的道德性，进行以德性为目的的榜样教育，发挥感性实践的重要作用，以自由和谐丰富集体主义道德原则的内涵，培育自由的道德人。  相似文献